Jingyan Han's research while affiliated with Peking University Health Science Center and other places

Publications (26)

Article
Ethnopharmacological relevance: Marsdenia tenacissima (Roxb.) Wight & Arn is a well-known traditional Chinese medicine for treating cancer. The anti-tumor effects of the water soluble component of M. tenacissima (MTE, M. Tenacissima Extract) have been intensely studied. However, the roles of microenvironmental cells in mediating the anti-tumor act...
Article
Ethnopharmacological relevance: Marsdenia tenacissima is a traditional Chinese medicine that is known to be effective in combating cancer as well as reducing blood pressure. The efficacy and mechanisms of Marsdenia tenacissima in treating cancer have been well described. However, the potential vasoactivities of Marsdenia tenacissima remain poorly...
Article
Objective: Chemokine-mediated neutrophil recruitment contributes to the pathogenesis of abdominal aortic aneurysm (AAA) and may serve as a promising therapeutic target. FAM3D (family with sequence similarity 3, member D) is a recently identified novel chemokine. Here, we aimed to explore the role of FAM3D in neutrophil recruitment and AAA developm...
Article
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The prevalence of non-alcoholic fatty liver disease (NAFLD), which is developed from hepatic steatosis, is increasing worldwide. However, no specific drugs for NAFLD have been approved yet. To observe the effects of Rho, a fraction from Rhodiola crenulate, on non-alcoholic hepatic steatosis, three mouse models with characteristics of NAFLD were use...
Article
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Background: Although drug-eluting stents have dramatically reduced the rates of restenosis and target lesion revascularization, they are associated with stent thrombosis (ST), a catastrophic event likely due to delayed endothelialization and chronic inflammation caused by the polymer and the metal scaffolds. To increase the safety and efficacy of...
Article
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Hemorrhagic stroke accounts for 10-15% of all strokes and is strongly associated with mortality and morbidity worldwide, but its prevention and therapeutic interventions remain a major challenge. Here, we report the identification of miconazole as a hemorrhagic suppressor by a small-molecule screen in zebrafish. We found that a hypomorphic mutant f...
Patent
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The present invention discloses a traditional Chinese drug consisting of Danshen extract and Sanqi extract, the pharmaceutical composition comprising the above traditional Chinese drug as an active component and the use thereof. The traditional Chinese drug consists of Danshen extract and Sanqi extract in a weight ratio of (2˜6):(0.5˜2). The invent...
Article
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Ischemic stroke and ischemia/reperfusion (I/R) injury induced by thrombolytic therapy are conditions with high mortality and serious long-term physical and cognitive disabilities. They have a major impact on global public health. These disorders are associated with multiple insults to the cerebral microcirculation, including reactive oxygen species...
Article
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Ischemia-reperfusion (I/R) injury model has been widely applied to the study of microcirculation disturbance. In this work, we used laser oblique scanning optical microscopy (LOSOM) to observe the microcirculation system in the mesentery of rat model. Utilizing a localized point-scanning detection scheme, high-contrast images of leukocytes were obt...
Article
Myoepithelial cells (MECs) mainly surround acini and intercalated ducts in the human salivary glands. The contraction of MECs provides the expulsive force to promote salivation. We previously found functional transient receptor potential vanilloid subtype 1 (TRPV1) was expressed in rabbit and human submandibular glands and increased saliva secretio...
Article
Transmembrane protein 166 (TMEM166) is a lysosomal/endoplasmic reticulum-associated protein found in various species where it acts as a regulator of programmed cell death, mediating both autophagy and apoptosis. In the present study, we investigated the role of TMEM166 following MCAO injury in rats to determine whether the structural damages follow...
Article
The blood-brain barrier (BBB) plays a vital role as both a physiologic and physical barrier in regulating the movement of water from the vasculature to the brain. During a subarachnoid hemorrhage (SAH), the BBB is disrupted by a variety of mediators, one of which can result in endothelial cell death. As a result, in the present study, we investigat...
Article
The initial mechanical tissue disruption of spinal cord injury (SCI) is followed by a period of secondary injury that increases the size of the lesion. Secondary injuries are associated with edema, inflammation, excessive cytokine release, excitotoxicity and cell apoptosis. 3,4-dihydroxyphenyl lactic acid (DLA) is one of the major water-soluble com...
Article
Various studies on focal cerebral ischemic models have implicated the direct activation and expression of matrix metalloproteinases (MMPs), especially MMP-9, as a key orchestrator of blood-brain barrier (BBB) disruption. Moreover, studies have shown that MMP-9 siRNA can protect the BBB from ischemia/reperfusion injury. In the present study, we inve...
Article
Hypoxia-inducible factor-1 (HIF-1) plays an essential role in cerebral ischemia as a proapoptotic factor. We hypothesized that HIF-1alpha siRNA can protect the brain from ischemic damage by inhibiting HIF-1alpha induced apoptotic pathway at the RNA level in a rat focal ischemic model. Results showed that treatment with HIF-1alpha siRNA reduced the...
Article
Full-text available
Determination of erythrocyte flow velocity is an important parameter for microcirculatory research. We established an off-line method for determining erythrocyte velocity in microcirculation by computer- ized dynamic grey scale analysis. In principle, the method is based on the dual-slit photometric technique (1), and updated by software image anal...
Article
RNA interference appears to have a great potential not only as an in vitro target validation, but also as a novel therapeutic strategy based on the highly specific and efficient silencing of a target gene. We hypothesize that MMP-9 siRNA can be effective as an MMP-9 protein inhibitor in a rat focal ischemia model. Male Sprague-Dawley rats (156) wer...
Article
Despite 2-methoxyestradiol (2ME2) and tricyclodecan-9-yl-xanthogenate (D609) having multiple effects on cancer cells, mechanistically, both of them down-regulate hypoxia-inducible factor-1alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF). We hypothesize HIF-1alpha plays an essential role in cerebral ischemia as a pro-apoptosis regula...

Citations

... Furthermore, Marsdenia tenacissima is a traditional Dai medicine antitumor drug. Thus, many scholars have studied its antitumor effects in depth [49][50][51][52]. Hu Y et al. found that the extract of Dai ethnomedicine Marsdenia tenacissima could promote lung cancer apoptosis by regulating the Ca 2+ /CaM/CaMK signaling pathway [49]. ...
... Calcium ions (Ca 2+ ), as a second messenger, play an important role in different types of cells involved in various CVD conditions, such as hypertension, arrhythmias, and myocardial infarction (Firth et al., 2013;Xia et al., 2016;Mustroph et al., 2017;Wilson et al., 2019). Under physiological conditions, regulation of intracellular Ca 2+ concentration ([Ca 2+ ]i) can effectively change the activity of vasodilation/contraction in vascular smooth muscle cells (VSMCs) (Marchand et al., 2012;Hao et al., 2019). Endothelial cells participate in the modulation of vascular tone by releasing vasoactive agents, such as nitric oxide (NO), which in turn alters [Ca 2+ ] i and maintains blood vessel homeostasis (Takeuchi et al., 2004;Filippini et al., 2019). ...
... These data, combined with earlier work in cerebral ischemia [65], suggest the crucial role of Sp1 in the regulation of Abcc8 expression across species [81,82,86]. Pharmacological inhibition and gene suppression of Hif is protective in cerebral ischemia/hypoxia, which corresponds with the role of Hif1 in upregulation of SUR1 in focal ischemia [87][88][89]. Similar results were found in the Rice-Vannucci model of neonatal hypoxia-ischemia, confirming neuroprotection [90,91]. ...
... The expression level of FAM3D is affected by nutritional status, with postprandial elevation and a reduction after fasting, and the secretion timing of FAM3D is contrary to that of glucagon (Cao et al., 2017). Some studies found that FAMSD can induce Mac-1-mediated neutrophil recruitment and strongly chemoattract human peripheral blood neutrophils and monocytes (Peng et al., 2016;He et al., 2018), which indicates that FAM3D may be regarded as a proinflammatory factor. Ting Cao et al. found that FAM3D inhibits glucagon secretion via MKP1-dependent suppression of ERK1/2 signaling (Cao et al., 2017). ...
... GLP-1 has been well established in reducing body weight and lowering blood sugar through inhibiting gastrointestinal peristalsis and gastric juice secretion and enhancing insulin secretion [24]. A variety of studies have confirmed that impaired glucose homeostasis was reflected by higher AUC of GTT and ITT [25,26]. Furthermore, the concentration of TG and TC in the feces of all mice was detected to confirm the regulation of metabolitin on lipid metabolism. ...
... This DDES was found to be as safe as the commercial BMS and SES, but did not show any enhancement of re-endothelialization in porcine arteries. 140 Nano-thin-films and their combination with drugs/ biologics Apart from the nanostructured topography generated on metallic surfaces, deposition of thin films on stents/substrates has also been widely examined. The concept of utilizing stent coatings was initially introduced as a means to mask the underlying stent surface, to prevent ion leaching from bare-metal stent surface into the bloodstream. ...
... Owing to their small size, rapid development and high fecundity, zebrafish larvae offer an attractive pre-clinical system for drug screening (Margiotta-Casaluci et al., 2019;Patton et al., 2021). Zebrafish brain haemorrhage models have previously been used for investigation to identify compounds that can inhibit cerebral bleeding (Yang et al., 2017). However, in this study, larvae were treated after ICH and screened for a reduction in brain injury, which offers a more translationally relevant approach to emergency treatment compared to bleed prevention. ...
... Lower IL-10 levels are related to one of increased risks in stroke. Guizhi Fuling Capsule significantly upregulate the level of IL-10 mRNA and protein expression and its receptor in rats with focal cerebral ischemia and reperfusion (I/R) (Sun et al., 2015). ...
... In studies of cardiomyopathy mechanisms, LSFM has been used to associate neural crest cell-derived cardiomyocytes with trabeculation and cardiac contractility (69)(70)(71)(72)(73)(74)(75), with minimal photobleaching and phototoxicity during time-lapse imaging. In addition, this method has been used to quantify the velocity of circulating blood cells (76) in a transgenic zebrafish model of tail injury, allowing for correlation of microcirculatory shear rates with vascular regeneration and providing clinical relevance to the use of selective-plane illumination (77). Due to the cyclical rhythm of myocardial contraction and relaxation, LSFM has marked potential to deliver 4-D reconstruction of dynamic AV valve leaflet movements and, after applying spatial registration between images captured at different time points (20), can provide further insights into the mechanosignal transduction of valvulogenesis. ...
... It is highly conserved in various vertebrates, indicating that it probably has significant functions in vertebrates. Studies have demonstrated that TMEM166 is mainly located to endoplasmic reticulum and its overexpression can induce cell autophagy and apoptosis [9][10][11][12]. Immunohistochemical or chip analysis has revealed that TMEM166 is predominantly expressed in endocrine tissues, especially in liver, stomach, esophagus, adrenal cortex and pituitary gland, indicating its important roles in these organs [13]. ...