Jingsheng Huang's research while affiliated with Nanyang Technological University and other places

Publications (15)

Article
Full-text available
Checkpoint immunotherapies have been combined with other therapeutic modalities to increase patient response rate and improve therapeutic outcome, which however exacerbates immune-related adverse events and requires to be carefully implemented in a narrowed therapeutic window. Strategies for precisely controlled combinational cancer immunotherapy c...
Article
Real‐time optical imaging of immune cells can contribute to understanding their pathophysiological roles, which remains challenging. Current chemiluminophores have issues of short half‐lives and low brightness, limiting their ability for longitudinal monitoring of immunological processes. To tackle these issues, we report benzoazole‐phenoxyl‐dioxet...
Article
Real‐time optical imaging of immune cells can contribute to understanding their pathophysiological roles, which remains challenging. Current chemiluminophores have issues of short half‐lives and low brightness, limiting their ability for longitudinal monitoring of immunological processes. To tackle these issues, we report benzoazole‐phenoxyl‐dioxet...
Article
Activatable phototheranostics holds promise for precision cancer treatment owing to the “turn‐on” signals and therapeutic effects. However, most activatable phototheranostic probes only possess photodynamic therapy (PDT) or photothermal therapy (PTT), which suffer from poor therapeutic efficacy due to deficient cellular oxygen and complex tumor mic...
Article
Activatable phototheranostics holds promise for precision cancer treatment owing to the “turn‐on” signals and therapeutic effects. However, most activatable phototheranostic probes only possess photodynamic therapy (PDT) or photothermal therapy (PTT), which suffer from poor therapeutic efficacy due to deficient cellular oxygen and complex tumor mic...
Article
Full-text available
Chemiluminescence (CL) is an emission phenomenon induced by chemical reaction. Different from the photoluminescence, CL is free from external excitation source, which is expected to show great advantages such as higher signal‐to‐background ratio (SBR) in bioimaging, and deeper tissue penetration in photodynamic therapy (PDT). This review introduces...
Article
Full-text available
Immunometabolic modulation offers new opportunities to treat cancers as it is highly associated with cancer progression and immunosuppressive microenvironment. However, traditional regimens using nonselective small-molecule immunomodulators lead to the off-target adverse effects and insufficient therapeutic outcomes. Herein a second near-infrared (...
Article
Chemiluminescence imaging is imperative for diagnostics and imaging due to its intrinsically high sensitivity. To improve in vivo detection of biomarkers, chemiluminophores that simultaneously possess near-infrared (NIR) emission and modular structures amenable to construction of activatable probes are highly desired, which however are rare. We her...
Article
Near‐infrared chemiluminophores with modular structures amenable to the construction of activatable molecular probes are synthesized, which turn on their chemiluminescence in the presence of the biomarker of interest, with a very long emission wavelength of above 750 nm. Abstract Chemiluminescence imaging is imperative for diagnostics and imaging...
Article
Full-text available
Despite the superior sensitivity of chemiluminescence over fluorescence, most chemiluminescence reporters only emit visible light, and have low water solubility, making them poorly equipped for in vivo imaging applications. Herein two near‐infrared (NIR) chemiluminescent reporters (NCRs) with high renal clearance for real‐time imaging of reactive o...
Article
A macromolecular reporter whose near‐infrared fluorescence is activated by a bladder cancer biomarker is reported by K. Pu and co‐workers in their Research Article (DOI: 10.1002/anie.201911859). The reporter undergoes efficient renal clearance and accumulates in the bladder, enabling both the real‐time in vivo imaging of bladder cancer and in vitro...
Article
Ein makromolekularer Reporter, dessen Nahinfrarot‐Fluoreszenz durch einen Blasenkrebs‐Biomarker aktiviert wird, wird von K. Pu et al. in ihrem Forschungsartikel (DOI: 10.1002/ange.201911859) beschrieben. Der Reporter wird effizient über die Nieren ausgeschieden, sammelt sich in der Blase und wurde zur Untersuchung von Blasenkrebs in lebenden Mäusen...
Article
Bladder cancer (BC) is a prevalent disease with high morbidity and mortality; however, in vivo optical imaging of BC remains challenging because of the lack of cancer‐specific optical agents with high renal clearance. Herein, a macromolecular reporter (CyP1) is synthesized for real‐time near‐infrared fluorescence (NIRF) imaging and urinalysis of BC...
Article
Bladder cancer (BC) is a prevalent disease with high morbidity and mortality; however, in vivo optical imaging of BC remains challenging because of the lack of cancer‐specific optical agents with high renal clearance. Herein, a macromolecular reporter (CyP1) is synthesized for real‐time near‐infrared fluorescence (NIRF) imaging and urinalysis of BC...
Article
Therapeutic enzymes hold great promise for cancer therapy; however, in vivo remote control of enzymatic ac-tivity to improve their therapeutic specificity remains challenging. This study reports the development of an organic semiconducting pro-nanoenzyme (OSPE) with a photoactivatable feature for metastasis-inhibited can-cer therapy. Upon near-infr...

Citations

... 微流控芯⽚(Microfluidic Chip) [1] ,⼜称芯⽚实验室(Lab-on-a Chip)或者⽣物芯⽚。微流控技术 [2] 由微 机电加⼯系统(Micro-Electro-Mechanical System,MEMS)发展⽽来,它是⼀种在微⽶级微管 [3] 中精确操纵 [4] 微量流体的技术⼿段,具有将⽣物、化学等实验室微缩到⼀个⼏平⽅厘⽶芯⽚中的基本功能(样品的制备、 分离、反应、检测等)。随着材料科学的发展,作为微流控芯⽚载体的材料也层出不穷,从硅、玻璃到纸基 [5] 、 ⽔凝胶 [6] 以及各类聚合物和纳⽶材料 [7,8] 。与此同时,微流控芯⽚的制备技术也蓬勃发展如丝⽹印刷,喷墨打 印、3D 打印 [9], [10] 等,⼀些⾼精尖的加⼯技术如⻜秒激光加⼯技术、双光⼦ 3D 打印技术也为⾼精密度的微流 控芯⽚的制作提供了更多可能性。 微流控芯⽚有着微型化、⾼灵敏度、⾼集成、⾼通量、反应快、检测时间短等技术优势,在⽣物医学研 究,合成分析 [11] ,司法鉴定等众多领域有着⼴泛的应⽤(可穿戴微流控设备 [12] 、体外医疗诊断 [13] 、仿⽣⽪肤 [14] 组织器官 [15] ,⽣化 [16] 与环境分析、单细胞分析 [17] 、核酸分析、药物筛选递送 [18] )。迄今为⽌,微型化、集成 化和智能化已经成为现代科技⼿段的⼀个重要趋势,在这样的⼤环境下微流控芯⽚的发展⼗分迅速,它的分 类⽅法丰富多样,可根据不同的分析检测⽅法可以将微流控芯⽚分为电化学检测法 [19] 、光谱分析法(核磁共 振 [20] 、化学发光分析法 [21] )、⾮标记检测法 [22] ,分离富集 [8] 等。 微流控芯⽚是医学领域新⼀代床旁诊断(Point of care testing,POCT) [23,24] 主流技术。POCT 可直接在被检 者身边提供快捷有效的⽣化指标,现场指导⽤药,使检测、诊断、治疗成为⼀个连续过程,对于疾病的早期 发现和治疗具有突破性的意义。基于微流控芯⽚技术的 POCT 仪器发展趋势是⼩型化、操作便捷,直接输⼊ 体液样本 [25] ,即可迅速得到诊断结果 [26] ,由医⽣指导⽤药。⽬前,市场上有多种即时诊断⽅法,仅有简单地 流动测试⼯作没有流体管理技术,⽽当测试复杂性增加时,微流控技术是⼀⻔不可或缺的技术。在治疗肿瘤 癌症的医疗领域⽅⾯,微流控技术同样⼤放异彩,如何使药物特异性杀死肿瘤细胞⽽不伤害正常细胞,这⼀ 直是个业界难题,代尔夫特理⼯⼤学 Jan H. van Esch 的课题组 [27] 将微流控设备中搭载⼀种具有双重功能的新 ⽉形微凝胶,研究发现这些微凝胶对肺癌细胞有⾼亲和、⾼选择性,可以达到肺癌化疗的效果并且减轻副作 ⽤对⼈体的伤害。微流控细胞/组织/器官操作芯⽚是哺乳动物细胞以及微环境操作最重要的技术平台,渴望 应⽤于代替各类动物模型,模拟⼈体内环境(⼈体、组织芯⽚ [28] ),⽤于药物筛查递送,研究药物毒理和药 理作⽤,⼈体耐药性 [29] 的产⽣机制甚⾄肿瘤、癌症的早期诊断治疗等。 总览了近两年来微流控芯⽚应⽤于医学领域的相关研究报道,我们发现很多新兴的研究进展还缺乏全⾯ 的综述。本⽂对这⼀在蓬勃发展的领域做出了⼀个较为全⾯的概括,不可置否的是微流控芯⽚技术给基因、 免疫、微⽣物和临床等医学领域带来了颠覆性的突破,也是以⼈⼯智能为核⼼的第四次⼯业⾰命下必然⾼速 推进现代科技发展的⼀⻔技术。随着柔性膜的迅速发展,基于多功能性复杂材料制作的微流控芯⽚技术使诸 多疾病的早期预防诊断治疗成为可能,并推动临床医学⾛向了个性化、智能化的诊疗。 图 1 医⽤微流控芯⽚的制备⽅法、检测⼿段和医学领域的多元应⽤ 意图 [30] . (b)基于激光打印技术制备 3D 纳⽶等离⼦体的示意图 [31] .(c)利⽤喷墨打印技术进⾏微电极集成的示意图 ...
... Different from NIF imaging, chemiluminescence imaging directly emit light through chemical reactions with little tissue scattering and no interference from self-luminescence. Therefore, chemiluminescence imaging is more sensitive and deeper tissue penetration depth than NIF [102][103][104][105]. For example, Pu et al. developed a dual-channel molecular optical imaging probe (MRPD) with chemiluminescence and NIF groups for O2 .− ...
... In DTZ and cisplatin-induced AKI, MRPD detected AKI 16 hours and 60 hours earlier than the SCR method, respectively. Recently, Huang et al. further developed a near-infrared-based chemiluminescence imaging (NCR1-2) for the diagnosis of early AKI [106]. NCR1-2 contained two functional groups, namely HPβCD to improve renal clearance efficiency and dicyanomethylene-4-hydro-pyran modified with sap dioxane as the near-infrared chemiluminescence moiety. ...
... Enzymatic therapy has been utilized to afford more durable response than small-molecule drugs for a broad range of diseases [43][44][45] . Our nano-immunocomplex represents a remotecontrolled enzymatic nanomedicine to reprogram the immunosuppressive TME precisely and persistently for cancer immunotherapy. ...