Jin-Wu He's research while affiliated with Northwestern Polytechnical University and other places

Publications (9)

Article
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Papilio machaon was assigned as the type species for all butterflies by Linnaeus and P. bianor is a congener but exhibits a great difference in morphology (especially larva and adult color pattern) and larval host plants from P. machaon. Thus, they are the ideal models to investigate genetic mechanisms underlying morphology and plasticity between c...
Article
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Swallowtail butterflies (Papilionidae) are a historically significant butterfly group due to their colorful wing patterns, extensive morphological diversity, and phylogenetically important position as a sister group to all other butterflies and have been widely studied regarding ecological adaption, phylogeny, genetics, and evolution. Notably, they...
Article
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Pupal color polyphenism in Papilio butterflies, including green, intermediate, or brown, is an excellent study system for understanding phenotypic plasticity. Previous studies suggested that development of brown pupae may be controlled by a hormone called pupal-cuticle-melanizing-hormone (PCMH) which is synthesized and secreted from brain-suboesoph...
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Butterflies are diverse in virtually all aspects of their ontogeny, including morphology, life history, and behavior. However, the developmental regulatory mechanisms underlying the important phenotypic traits of butterflies at different developmental stages remain unknown. Here, we investigated the developmental regulatory profiles of butterflies...
Article
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Firefly adult bioluminescence functions as signal communication between sexes. How sympatric sibling species with similar glow pattern recognize their conspecific mates remains largely unknown. To better understand the role of the luciferases of sympatric fireflies in recognizing mates, we cloned the luciferase genes of three sympatric forest dwell...
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The pH‐insensitive beetle luciferases cloned from Rhagophthalmidae, Phengodidae, and Elateridae exhibit great potential application as reporter assays for monitoring gene expression. At present, however, only one luciferase has been reported from the enigmatic and predominantly Asian distributed luminous family Rhagophthalmidae. Here, we cloned the...
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The new subfamily Sinopyrophorinae within Elateridae is proposed to accommodate a bioluminescent species, Sinopyrophorus schimmeli Bi & Li, gen. et sp. nov., recently discovered in Yunnan, China. This lineage is morphologically distinguished from other click-beetle subfamilies by the strongly protruding frontoclypeal region, which is longitudinally...
Article
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Lamprigera (Lampyridae) is a small genus with only 17 species distributing in Asian countries. Its larviform females and alate males can produce continuously strong yellow‐green light at night. However, no luciferase gene was reported for this genus and its subfamily‐level phylogenetic position still remains uncertain. Here, we cloned the luciferas...
Article
Full-text available
Eukaryotic genome size data are important both as the basis for comparative research into genome evolution and as estimators of the cost and difficulty of genome sequencing programs for non-model organisms. In this study, the genome size of 14 species of fireflies (Lampyridae) (two genera in Lampyrinae, three genera in Luciolinae, and one genus in...

Citations

... Developmental studies have been done on several butterfly species, including Vanessa cardui [10], Pieris rapae [11], Bicyclus anynana [12], and many others. The genome of the nearest species of P. bremeri, P. apollo, Papilio xutus, Papilio machaon and P. bianor have been studied recently [13][14][15]. In addition, in recent times we have identified antimicrobial peptide candidates in P. bremeri against the causative agent of periodontitis, Porphyromonas gingivalis [16]. ...
... Developmental studies have been done on several butterfly species, including Vanessa cardui [10], Pieris rapae [11], Bicyclus anynana [12], and many others. The genome of the nearest species of P. bremeri, P. apollo, Papilio xutus, Papilio machaon and P. bianor have been studied recently [13][14][15]. In addition, in recent times we have identified antimicrobial peptide candidates in P. bremeri against the causative agent of periodontitis, Porphyromonas gingivalis [16]. ...
... The typical representatives have a compact body and a pro-mesothoracic clicking mechanism (Costa et al., 2010); however, this group also includes several soft-bodied lineages (Kundrata and Bocak, 2019). Despite recent progress in understanding the composition, phylogeny, and classification of Elateridae, all these aspects remain open to further study (Kundrata et al., 2018a;Bi et al., 2019;Kundrata et al., 2019b;Kusy et al., 2021;Douglas et al., 2021). The most recent study on the phylogeny of Elateridae (Douglas et al., 2021) showed that the Lampyridae and related bioluminescent families may be in fact derived click beetles, which means that the widely delimited Elateridae clade comprises more than 13 500 extant species worldwide (Costa et al., 2010;Douglas et al., 2021). ...
... Second, the luciferase fragments were recovered by digesting the three plasmids with NdeI and BamHI and then cloned into pColdI expression vector (Takara, Japan) to yield plasmids (named as pColdI-Dnu_Luc, pColdI-Dpe_Luc, and pColdI-DL9_Luc). Next, the expression, purification, and measurement profiles of three recombinant proteins were referenced to Liu et al. [18]. Briefly, plasmids (pColdI-Dnu_Luc, pColdI-Dpe_Luc, and pColdI-DL9_Luc) were transformed into Escherichia coli BL21 (DE3, Tiangen, China) and cultured in LB medium (Sangon Biotech, China). ...
... Genome analysis has been used to understand the evolutionary processes that drive the diversification and the evolution of the bioluminescence in Elateroidea (Braham and Wenzel, 2001;Day et al., 2004;Amaral et al., 2019a;2019b). The genome size estimation in Elateroidea based on flow cytometry was also performed for all bioluminescent families (Hanrahan and Johnston, 2011;Liu et al., 2017;Lower et al., 2017). These studies suggested that the genome sizes among species of this superfamily range from 0.4 Gb to 2.2 Gb, and identified a positive relationship between the genome size and the amount of repetitive DNA. ...