Jin Feng's research while affiliated with Leibniz Universität Hannover and other places

... The genetic engineering of fungal natural product biosynthesis genes and gene clusters constitutes the last focus of our SI. Feng et al. engineered Aspergillus oryzae for the heterologous expression of bacterial polyketide synthase genes, having a modular architecture [57]. ...

... The in vitro cloning of a large size of DNA has remained a key challenge of this technique. A recent CRISPR/Cas9 system was reported, for the first time, for capturing entire groups of BGCs in filamentous fungi [84]. In this system, the genomic DNA extracted from fungi was cleaved by RNA-guided Cas9 endonuclease in vitro, and in combination with in vivo yeast assembly, the entire BGCs were inserted into vectors for heterogeneous expression. ...

... Compounds 1-7 were evaluated for their inhibition of two wheat-pathogenic Fusarium graminearum ACCC39334 and Gaeumannomyces graminis ACCC38864. T sults showed only compounds 1, 2, and 6 were active against one of the two path The two known compounds, brasilane A (6) and harzianone A (7), were identified by comparison of their spectroscopic data with those reported in the literature [28][29][30]. Compound 6 features a brasilane skeleton that has been rarely discovered from Trichoderma [31,32]. It is the first brasilane aminoglycoside from Trichoderma. ...

... To address this issue, we utilized AmAT7-3 to acetylate eight medicinally important saponins, including astragaloside IV (1), astragaloside VII (2), ziyuglycoside II (3), bufalin 3-O-β-Dglucoside (4), cinobufagin 3-O-β-D-glucoside (5), 20(R)-ginsenoside Rh1 (6), glycyrrhetinic acid 3-O-β-D-glucuronide (7), and chikusetsu saponin IVa (8). The bioactivities of these compounds were shown in Fig. 5a [35][36][37][38][39][40][41] , where some of these original compounds or their derivatives, such as 1, 4, 5, 6, and 7, are currently undergoing clinical trials as drug candidates (NCT: 01553643, 00837239, 03843229, 00815763, and 05570110). The reaction mixtures were analyzed by UHPLC/MS, revealing that AmAT7-3 could effectively catalyze the regioselective acetylation of triterpenoid and steroidal saponins but not their aglycones ( Supplementary Figs. ...

... Feng and co-workers identified phenolic glycosyltransferase MhGT1 from the filamentous fungus Mucor hiemalis which exhibited promising capability for regio-and stereospecific Oglycosylation of different phenolic compounds [19] . By examining 93 compound library of phenols from Traditional Chinese Medicinal herbs as substrates, they revealed that MhGT1 exhibited very broad substrate specificity and catalyzed glycosylation of 72 structurally diverse drug-like scaffolds and sterols using uridine diphosphate (UDP) glucose as a sugar donor. ...

... (Fig. 1a) 10 . Among them, liquiritin apioside (liquiritigenin 4′-O-apiosyl(1→2)-glucoside) shows potent antitussive activities 11 .Currently, flavonoid apiosides are mainly obtained through extraction and purification from plants. ...

... First, we obtained a total of 33 compounds from SWFs ( Fig. 1), mainly PIFs and GPIFs, which constituted the major components in SWFs. In addition to 10 known PIFs, 6,8-diprenylorobol(24) 20 , lupalbigenin (25) 21 , isolupalbigenin (26) 22 , auriculasin (27) 23 , 4′-Omethylerythrinin C (28) 24 , lupiwighteone (29) 25 , erysenegalensein E (30) 26 , millewanins H (31) 27 , isoerysenegalensein E (32) 28 and millewanins G (33) 27 , regarded as the main secondary metabolites in CTLs, 23 GPIFs were also obtained (1-23), including 21 GPIFs not described before to the best of our knowledge, silexcrins A-U (1-21), and two known ones, lupiwighteone 7-β-D-glucoside (22) 29 and genisteone (23) 30 , which are O-glycosylated derivatives mainly introducing one or two glucoses into 7,3′,4′-hydroxyls at the prenylated isoflavone skeleton. In addition, two trivial pairs of epimers 13-16 were also obtained and identified with 2″R, 2″S, 3″′S, and 3'″′R carbon stereo-centres by calculated ECD (Fig. 2). ...