Jih-Heng Chen’s research while affiliated with National Kaohsiung University of Science and Technology (NKUST) and other places

... [10,11] Traditional wastewater treatment methods while effective to some degree, often fall short in completely removing these nutrients, especially in the context of industrialscale contamination, additionally conventional treatments energy-intensive and costly prompting the search for more sustainable alternatives. [12][13][14] Phytoremediation has emerged as promising eco-Phytoremediation of Fertilizer Manufacturing Effluent Contaminated with Nitrogen and Phosphorus Fertilizers Using Common Reed friendly approach to address the challenges of nutrientrich wastewater. [15] This biological treatment method harnesses the natural ability of plants to absorb, transform, and store contaminants from their environment, [16,17] phytoremediation offers several advantages over traditional physicochemical treatments, including lower operational costs, reduced energy consumption and the potential for resource recovery through biomass utilization. ...

Reference:

Phytoremediation of Fertilizer Manufacturing Effluent Contaminated with Nitrogen and Phosphorus Fertilizers Using Common Reed

... In this context, the United Nations (UNs) established 17 Sustainable Development Goals (SDGs) targeting their implementation by 2030. Microalgae can assist in fulfilling this objective by providing a wide portfolio of bioproducts with promising applications in the biofuel, food, and nutraceutical industries [1]. A microalgae-based biorefinery concept can contribute to a circular economy scenario, highlighting the significance of these microorganisms in creating a greener future, leveraging their potential as main resources for the production of thirdgeneration biofuels [2]. ...

Reference:

Strategic bioprocessing of A. protothecoides and C. sorokiniana using renewable feedstocks for targeted bioproduct and biodiesel generation

... Fucoxanthin content in microalgae varies with nutrient availability. Chen et al. (2024) reported that fucoxanthin content in Pavlova sp. peaked at 36.47 mg/g with 400-mg/L NaNO 3 , compared to 33.13 mg/g at 200 mg/L and 34.76 mg/g at 800 mg/L. ...

Reference:

Nutrient-mediated changes in growth, biochemical composition, and biosilica deposition in Cyclotella cryptica

... In a sewage treatment plant, an optimized microalgae-bacteria symbiosis system could achieve impressive removal rates of contaminants such as chemical oxygen demand and ammonia nitrogen, reaching up to 95%, while having a removal efficiency of total nitrogen and total phosphorus as high as 65% (Qv et al., 2023). Similar contaminant removal efficiency was also achieved in a Chlorella sorokiniana AK −1 -mixed bacterial consortia system in treating dairy manure wastewater (Chang et al., 2023). This symbiosis consortium was able to self-supply nutrients for growth even in inferior environments, such as nutrient starvation, extreme pH levels, high osmotic stress, and the presence of toxic compounds. ...

Reference:

KEIO knockout collection reveals metabolomic crosstalk in Chlorella spp.‐ Escherichia coli co‐cultures

... The fermentation process in SSF requires sufficient oxygen supply in the liquid phase, achieved through aeration and intermittent medium stirring, alongside maintaining optimal conditions for temperature, pH, ionic strength, and nutrients to ensure maximum yield 79 . This kind of fermentation offers advantages due to its relatively low capital investment and minimal waste generation, delivering economic and environmental benefits 80 contemporary fermentation-based research is increasingly favoring the SSF approach to enhance techno-economic and sustainability benefits 81 . However, SSF encounters substantial challenges requiring significant innovations to unlock its full commercial potential 82 . ...

Reference:

Recent advances and challenges in single cell protein (SCP) technologies for food and feed production

... This fact suggests that (in dark conditions) this growth phase is a potential source of this xanthophyll. Also, according to our results, [42] showed that with distinct culture media, such as cultures in heterotrophic [43] or mixotrophic systems [44,45], the production of lutein is substantially significant and may be considered as a potential source of commercial output of this pigment. ...

Reference:

Trade-Off Between Growth Regimes in Chlorella vulgaris: Impact on Carotenoid Production

... In a semi-continuous operation of swine WW, the microalga Chlorella sorokiniana, within an algal-bacterial consortium, exhibited a growth rate of 0.12 d − 1 in 50% WW. When exposed to 90% WW, the same species demonstrated an increased growth rate of 0.37 d − 1 , attributed to the growth-promoting effects of bacteria, as highlighted by Chen et al. in 2022. Notably, although not sterilised, the synthetic WW in this study was likely not as heavily contaminated as normal WW, potentially reducing the presence of the stimulatory bacteria mentioned. ...

Reference:

Removal of antibiotics and their impact on growth, nutrient uptake, and biomass productivity in semi-continuous cultivation of Auxenochlorella protothecoides

... A produtividade de etanol foi de 7,387 g/L.h, e a produtividade total, após 8 horas de fermentação foi de 43,9 g/L, dados que, segundo os autores, sugerem elevado potencial para produção do bioetanol de forma economicamente viável. A aeração do cultivo com CO2 a uma concentração de 2 % elevou a concentração de biomassa em até 20 vezes, e sua influência foi considerada crucial, pois aumenta a biodisponibilidade de carbono para fixação fotossintética (Condor et al., 2022). Megawati et al. (2022) desenvolveram um processo de hidrólise enzimática com glucoamilase atingindo um rendimento de 80 % de glicose em condições de 3 % em volume de enzima adicionada em pH 5 e temperatura de 80 °C por 5 horas, fermentada posteriormente por Saccharomyces cerevisiae. ...

Reference:

Microalgas e a produção de energia de baixo carbono: uma alternativa sustentável aos combustíveis fósseisMicroalgae and low carbon energy production: a sustainable alternative to fossil fuelsMicroalgas y producción de energía baja en carbono: una alternativa sostenible a los combustibles fósiles

... This demonstrates the effectiveness of continuous hydrothermal liquefaction (HTL) processes in the production of biofuels. In a similar study, Kumar et al. [111] investigated the direct hydrothermal liquefaction (HTL) of microalgae to produce bio-oil using a high-pressure batch reactor under subcritical water conditions. Three different microalgae samples, namely Chlorella vulgaris, Botryococcus braunii, and Scenedesmus quadricauda, were examined under hydrothermal liquefaction with different water concentrations (1:6, 1:7, 1:8, 1:9 & 1:10 ratio) at a temperature range of 200-320 °C, pressure of 60 bars, and a reaction time of 30 minutes. ...

Reference:

Sustainable Biotechnology Approaches Using Chlorella vulgaris's Biomass and Lipids as Biofuel Feedstock

... As a result, the expense of the growth medium's constituents is unnecessary. In addition to the elements we mentioned, external factors such as light, temperature, and pH may also be necessary for the growth of microalgae [4]. Like all living things, microalgae die when they cannot adapt to environmental conditions. ...

Reference:

Investigation of The Bioethanol and Antioxidant Potential of Borodinellopsis texensis Grown in Wastewater under Various Copper Concentrations