Jie Zhang’s research while affiliated with Southwest Hospital and other places

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Publications (390)


Enrichment of Nitrosocosmicus-AOA in situ and their vertical distribution characteristics in aerated biofilters
  • Article

April 2025

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4 Reads

Environmental Research

Kehuan Guo

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Luyao Teng

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Jie Zhang

Symptoms of naturally infected fish (left) and colonies on LB plate (right). (A) The external symptoms of diseased fish. (B,C) Symptoms of internal organs. (D) Colonies on LB plate. Black arrow shows hemorrhaging along the body surface, and red arrow shows the flatulence in intestine.
Detection of virulence genes in A. hydrophila (A), A. veronii (B), P. shigelloides (C), and C. freundii (D). M: Maker.
Symptoms on the epithelial surface and internal organs of experimentally infected fish. (A1,A2), A. veronii infection, (B1,B2), A. hydrophila infection, (C1,C2), P. shigelloides infection, (D1,D2), C. freundii infection, (E1,E2), mixed infection.
Susceptibility of bacterial isolates to antibiotics.
Mixed Infection in Common Carp (Cyprinus carpio) Caused by Aeromonas veronii, Aeromonas hydrophila, Plesiomonas shigelloides, and Citrobacter freundii
  • Article
  • Full-text available

March 2025

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45 Reads

Aeromonas veronii, Aeromonas hydrophila, Plesiomonas shigelloides, and Citrobacter freundii are opportunistic pathogens that are widely distributed in aquatic environments and fish populations, causing fish diseases under stressful conditions. In this study, bacteria were isolated from a group of diseased common carp, Cyprinus carpio, with symptoms of hemorrhaging along the body surface, abdominal distention, and flatulence in the intestine. According to the results of biochemical characteristics and 16S rRNA sequencing analyses, the isolates were identified as A. veronii, A. hydrophila, P. shigelloides, and C. freundii. The virulence genes from these four representative bacterial strains were amplified. The experimentally co-infected fish showed similar symptoms to those observed in the naturally infected common carp. The LD50 of C. freundii, P. shigelloides, A. veronii, A. hydrophila, and the mixture group were 1.95 × 10⁴, 4.74 × 10⁴, 5.12 × 10⁴, 1.53 × 10⁵, and 5.41 × 10⁴ CFU, respectively. The antibiotic resistance results showed that P. shigelloides, A. veronii, and A. hydrophila were most sensitive to ceftizoxime, florfenicol, enrofloxacin, and norfloxacin. C. freundii was most sensitive to ceftizoxime and gentamicin.

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Identification, Characterization, and Pathogenic Investigation of Aeromonas veronii, Aeromonas hydrophila, Plesiomonas shigelloides, and Citrobacter freundii from a Case of Natural Mixed Infection in Common Carp (Cyprinus carpio)

January 2025

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11 Reads

Aeromonas veronii, Aeromonas hydrophila, Plesiomonas shigelloides, and Citrobacter freundii were opportunistic pathogen widely distributed in water environment and fish population, causing fish diseases under stressful conditions. In this study, bacteria were isolated from diseased common carp Cyprinus carpio with symptoms of hemorrhage on body surface, abdominal distention, and flatulence in the intestine. According to the results of biochemical characteristics, 16S rRNA sequencing analyses, the isolates were identified as A. veronii, A. hydrophila, P. shigelloides, and C. freundii. The experimentally infected fish showed identical symptoms as observed in the naturally infected common carp. The LD50 of C. freundii, P. shigelloides, A. veronii, A. hydrophila, and mixture group were 1.95×104, 4.74×104, 5.12×104, 1.53×105, and 5.41×104 respectively. Antibiotic resistance results showed that P. shigelloides, A. veronii, and A. hydrophila were sensitive to streptomycin, enrofloxacin, florfenicol, gentamicin, kanamycin, neomycin, norfloxacin, co-trimoxazole, ceftizoxime, and resistant to ampicillin. C. freundii was sensitive to streptomycin, gentamicin, and ceftizoxime, moderately susceptible to kanamycin, neomycin, norfloxacin, and ampicillin, and resistant to enrofloxacin, florfenicol, tetracycline, and co-trimoxazole.


Citations (39)


... Through the integration of proteomic, metabolomic, and lipidomic data, the reference spectrum of HCC subtypes has been successfully established, which may facilitate more precise and personalized treatment strategies for HCC patients [16]. In addition, certain studies have elucidated the molecular typing, prognostic features, and therapeutic targets of HCC patients from the perspective of proteomics, phosphoproteomics, and ubiquitomics, providing a significant theoretical foundation for the prognosis and assessment of HCC [17]. Although molecular typing presents novel opportunities for precision treatment of HCC, several challenges persist, including the accurate typing of HCC, the appropriateness of perioperative immunological application, the differential benefits of immunotherapy for HCC patients with varying etiologies, and the paucity of data from large-scale clinical trials. ...

Reference:

Integrated multi-omics analysis and machine learning refine molecular subtypes and clinical outcome for hepatocellular carcinoma
Integrated ubiquitomics characterization of hepatocellular carcinomas
  • Citing Article
  • September 2024

Hepatology

... NO 3 showed a lower removal rate than nitrite even though it achieved the same maximum efficiency of 80%. Findings from Zhang et al. (2024) suggest that the rate at which NO2 is reduced can be 1.63 times higher in comparison to the rate of nitrate reduction.. Using wetland system achieves better efficiency than shell powder, namely 82 -99%. However, the efficiency of shell powder can be better than the use of several types of adsorbents (<80%), namely polymer hydrogel (50%) (Kioussis et al., 2000), rice husk (6.13%) (Abdou et al., 2018), microalgae Picochlorum maculatum (46.4%) (Kumar et al., 2016) or a combination of wetland systems, shellfish and fish (18.55%) (Saiya and Katoppo, 2015). ...

Nitrate and nitrite utilization during denitrifying phosphorus removal: Electron acceptor preference and feasible process combinations
  • Citing Article
  • July 2024

Bioresource Technology

... The enteric defense mechanism plays an integral role in preserving gut homeostasis and protecting it from notorious pathogens, as evidenced during cryptosporidiosis [47][48][49][50][51]. Cryptosporidium spp. ...

Cryptosporidium parvum disrupts intestinal epithelial barrier in neonatal mice through downregulation of cell junction molecules

... Total RNA was prepared and used for the RNA-seq library construction, and subsequently sequenced on an Illumina Novaseq platform and 150 bp pairedend reads were generated [33]. The human genome version of hg38 was used as reference. ...

A cholangiocyte organoid system for Cryptosporidium parvum cultivation and transcriptomic studies of biliary cryptosporidiosis
  • Citing Article
  • May 2024

hLife

... We then detail procedures for the recovery and propagation of frozen parasites using enteroids. For complete details on the use and execution of this protocol, please refer to Deng et al. 1 ...

Cultivation, cryopreservation, and transcriptomic studies of host-adapted Cryptosporidium parvum and Cryptosporidium hominis using enteroids

iScience

... Starting from chitosan/iron sludge beads and using a pyrolytic procedure, magnetic chitosan carbon (MCC) was fabricated and used in the adsorption of Sb(III) from synthetic aqueous solutions [28]. Using this adsorbent, the variation of the pH from 3 to 10 had a negligible effect on the removal of this element. ...

Facile Preparation of Magnetic Chitosan Carbon Based on Recycling of Iron Sludge for Sb(III) Removal

... Both bare and starch-coated magnetic nanoparticles were synthesized using the co-precipitation method and employed for As(V) adsorption. Starch was used to coat the magnetite nanoparticles, enhancing the stability and dispersion of the adsorbent while also boosting its adsorption performance (Zeng, 2024). A study on the one-step co-pyrolysis of DWTR and corn stalk to produce magnetic adsorbents at varying temperatures revealed that increasing the pyrolysis temperature from 400 to 1000 • C enhanced both the saturation magnetization and SSA. ...

A comprehensive study of As(V) removal by starch-coated magnetite nano-adsorbent based on waste iron sludge
  • Citing Article
  • March 2024

Reactive and Functional Polymers

... Excellent phosphorus adsorption of LDH/biochar composites has been reported (Yang et al., 2019;Zhang et al., 2022a). Co-precipitation is the most widely used method for preparing LDH/biochar composite (Zeng et al., 2024;Zubair et al., 2021). The co-precipitation method usually requires strict pH regulation, and the rapid and uncontrollable co-precipitation leads to low crystallinity LDH with uneven particle size (Keyikoglu et al., 2022). ...

Activation of peroxymonosulfate by sludge-derived magnetic biochar for pollutant removal: Performance, applicability, and synergetic mechanism of iron species and carboxylated biochar
  • Citing Article
  • February 2024

Chemical Engineering Journal

... Studies have shown that persulfate [27], percarbonate [28], H 2 O 2 [29], periodate (PI, IO 4 -) [30] and peracetic acid [31] can effectively participate in Fe(VI)-based processes, enhancing pollutant degradation through different reaction mechanisms. PI stands out among the other oxidants used in water treatment owing to its high oxidation potential of +1.60 V [32] and ease of storage and transportation [33]. It can be activated to produce reactive oxygen species through various methods, such as photolysis [34], homogeneous activation (e.g., by reducing agents [35]), and heterogeneous activation (e.g., by carbon nanotubes [36] and iron-based materials [33]). ...

Iron-based materials for activation of periodate in water and wastewater treatment processes: The important role of Fe species
  • Citing Article
  • February 2024

Chemical Engineering Journal

... In our study, SKP2 inhibited p53 acetylation by competing with p53 for binding to p300, thereby leading to p53 inactivation. Moreover, OC2 knockout or SKP2 cell apoptosis [26][27][28]. Our studies involving OC2 knockdown and knockout revealed a significant decrease in the expression of the antiapoptotic proteins PARP and Bcl2, alongside an upregulation of the proapoptotic protein Bax. ...

Elevated expression of WSB2 degrades p53 and activates the IGFBP3-AKT-mTOR-dependent pathway to drive hepatocellular carcinoma

Experimental and Molecular Medicine