March 2025
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3 Reads
Ultrasonics Sonochemistry
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March 2025
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3 Reads
Ultrasonics Sonochemistry
February 2025
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4 Reads
LWT
January 2025
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2 Reads
January 2025
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6 Reads
Tiger nuts were enzymatically hydrolyzed by Alcalase and then separated and purified by ultrafiltration classification and Sephadex G-15 fractionation to obtain tiger nut peptides. Their chemical antioxidant activities and cytoprotective functions on HepG2 and Caco-2 cells were systematically evaluated in this study. The tiger nut peptides (TNP) were found to perform excellent antioxidant activity supported by their chemical and cell antioxidant behaviors, amino acid composition, and morphological observation. Higher 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity (DPPH• RSA, 64.05–124.07%) and ferric ion-reducing antioxidant power (FRAP, 0.17–1.78 μmol/mL) were observed in the TNP with more hydrophobic amino acids (41.77 ± 1.36 g/100 g) compared with traditional soybean and peanut peptides. Furthermore, the peptides from tiger nut (TNP, TNP-4, T1, T2, T3) could effectively protect H2O2-induced HepG2 and Caco-2 cells from oxidative damage by enhancing endogenous antioxidant enzyme activities and reducing oxidative stress levels, especially the T3 peptides purified from the fraction less than 1 kDa molecular weight. The catalase, superoxide dismutase, and glutathione peroxidase activities significantly increased, and the contents of intracellular reactive oxygen species and malondialdehyde decreased. This study highlights the potential of the peptides from tiger nuts as antioxidant ingredients for food applications.
January 2025
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10 Reads
LWT
December 2024
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16 Reads
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3 Citations
Ultrasonics Sonochemistry
Biofilms can persist in food industry environments leading to repeated cross-contamination, thus threatening human health. Slightly acid electrolyzed water (SAEW) and ultrasound have emerged as environmentally-friendly antimicrobial agents. However, their bactericidal efficacy is not high when used alone. In the present study, the effect of ultrasound combined with SAEW on removing Listeria monocytogenes biofilms from a glass surface was evaluated. Listeria monocytogenes biofilms were treated by immersion in sterilized deionized water (control treatment), immersion in sterilized deionized water combined with ultrasound (US treatment), immersion in SAEW (SAEW treatment), immersion in SAEW combined with ultrasound treatment (SAEW + US treatment), immersion in SAEW followed by immersion in sterilized deionized water combined with ultrasound (SAEW − US treatment), and immersion in sterilized deionized water combined with ultrasound followed by immersion in SAEW (US − SAEW treatment). The results showed that treating biofilms by US − SAEW treatment led to the lowest number of Listeria monocytogenes, the lowest amount of biofilm remaining on the glass slide were visualized by crystal violet staining and scanning electron microscope, and a minimal content of extracellular polymeric substances. Overall, the highest biofilm removal efficacy was observed for the US − SAEW treatment.
December 2024
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2 Reads
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1 Citation
Talanta
December 2024
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5 Reads
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1 Citation
Food Bioscience
November 2024
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11 Reads
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1 Citation
It is inevitable for polyphenols and polysaccharides to interact during food preparation. Modifications in microstructure can lead to changes in the physical and chemical properties of food systems, which in turn may influence the nutritional characteristics and functional activities of the food. Recent studies have shown that, in addition to traditional Chinese medicine compounds, certain natural polysaccharides and polyphenols exhibit significant anti-inflammatory and antioxidant properties. These compounds are also associated with beneficial therapeutic effects for the prevention and treatment of acute lung injury. The objective of this study was to examine the synergistic antioxidant effects of chlorogenic acid (CA) and Lycium barbarum polysaccharide (LBP) in various ratios, along with their combined antioxidant and anti-inflammatory effects on LPS-induced inflammation in rat alveolar macrophages. Using the Combination Index (CI), which quantifies the synergistic or antagonistic effect of two substances, all four combinations showed synergistic antioxidant properties over a range of concentrations by in vitro antioxidant property experiments. However, based on comparing them, the four group ratios exhibited the highest antioxidant activity of the infusion at CA:LBP = 1:7, indicating synergistic interactions (CI < 1). In addition, the antioxidant and anti-inflammatory effects of the CA-LBP complex were observed to alleviate cellular inflammatory injury by reducing LPS-induced nitric oxide and reactive oxygen species production and inhibiting the release of inflammatory factors such as TNF-α and IL-6.
November 2024
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4 Reads
Analytical Methods
Long-term consumption of enrofloxacin (ENRO) excessive residual food, may cause the accumulation of ENRO in the human body, further damaging human health. In this study, quantum dot-based biomimetic fluorescence immunoassays...
... TNP was prepared from tiger nuts according to the method described by Alemán et al. [26] with modification and we used the previous laboratory results [16]. Briefly, the defatted tiger nut meal was filtered through a 150 µm standard sieve, combined in a ratio of 1:6 (powder/solution, w/v) with deionized water, and the protein solubility was determined using the Coomassie Brilliant Blue between pH 7.0 and 11.0. ...
December 2024
Food Bioscience
... LBP, as a distinctive bioactive metabolite in L. barbarum L., has a complex chemical structure comprising various monosaccharides, including mannose, xylose, galacturonic acid, glucose, galactose, and arabinose (Yin et al., 2024). These sugar chain structures endow LBP with unique biological activities, including immune regulation, antioxidant properties, hypoglycemic effects, and lipid-lowering capabilities, which are essential to the pharmacological effects of L. barbarum L. Wu D. et al., 2024). ...
November 2024
... Listeria monocytogenes (LM), one of the four major foodborne pathogens, is widely found in diversified contaminated foods, such as seafood, meat, raw milk, fruits, and vegetables [1]. LM can form biofilms to enhance its viability in adverse environments, for example, low temperature, nutrient deficiency, and pH change, etc. [2]. ...
October 2024
... The microstructural changes during processing significantly affect the quality attributes of dried foods, including rehydration, color, texture, flavor, and nutrition (Niamnuy et al., 2014). The variations in the microstructure of the fresh and dried peels are demonstrated in Fig. 4. The microstructure variation may be attributed to pretreatment on the material structure and water migration during drying (Zhao et al., 2024). The microstructure of the HAD peels without pretreatment was tight, and the voids were not evenly distributed. ...
July 2024
Journal of Food Process Engineering
... Methods for extracting components from natural sources include drying, maceration [9], ultrasound-assisted extraction [10], microwave-assisted extraction [11], and superficial fluid extraction [12]. Drying is a simple, low-cost, and scalable extraction method that enables the efficient obtention of secondary metabolites by applying mild (20-60 • C) or elevated (>70 • C) temperatures [13,14], and freezing [15], oven [16], or vacuum drying [17]. Even though the variability in such experimental settings can influence the bioactivity and functional properties of the extracted substances, the importance of drying extraction has been reflected over the last decade in the obtention of compounds of highly valuable antitumoral [18], antidiabetic [19], antioxidant, and anti-inflammatory compounds [20]. ...
May 2024
LWT
... The steps involved are Fluorescent immunoassay (FIA) FIA involves the use of fluorescently labeled antibodies or antigens. The fluorescence intensity correlates with the concentration of melamine in the sample (Hu et al., 2024). The key steps involved are • Sample Preparation: Similar to other immunoassays, involving the use of antibodies or antigens labeled with fluorescent dyes. ...
April 2024
Food Bioscience
... g for MC and M6, respectively). This weight increase may be due to the higher fibre content of almond-skinenriched muffins, which improved moisture retention during baking [29]. ...
March 2024
Food Chemistry X
... This phenomenon may be attributed to the increased membrane rigidity resulting from surface modification, which led to a more ordered arrangement of the Lips bilayer and an elevation in the phase transition temperature. This observation aligns with previous findings regarding Lips membrane fluidity [44]. In conclusion, the co-modification of CS and LF significantly enhanced the phase transition temperature of the Lips system. ...
January 2024
International Journal of Biological Macromolecules
... It is known that ECAS fractions are capable of intensifying biocatalytic processes in food technologies [15]. ECAS intensify the germination of seeds and grains of plants, including during hydroponic cultivation, due to the activation of redox, hydrolytic and other classes of enzymes involved in this process [16][17][18]. The treatment of green buckwheat grains during germination with slightly acidic electrolysis water (pH 5.83, ACC 20.3 mg / l) contributed to a more intensive accumulation of gamma-aminobutyric acid (GABA) and rutin in the seedlings.It is believed that the accumulation of GABA and rutin in sprouted buckwheat treated with ECAS is due to an increase in the activity of glutamic acid decarboxylase and phenylalanine monialiase, that is, the effect on the secondary metabolic pathway of phenylpropanoids [16]. ...
November 2023
... To address these challenges, various pretreatment methods combined with drying processes have been investigated to improve efficiency and product quality. For instance, microwave pretreatment (350 W) followed by HA (50 °C) achieved the highest PhGs retention [9], while ultrasound-assisted vacuum far-infrared drying significantly increased PhGs content compared to natural air drying [10]. Additionally, freezing pretreatment (−20 °C for 7 days) combined with hot air impingement drying (60 °C, 6 m/s) increased PhGs content by 13.9% [11]. ...
August 2023
Industrial Crops and Products