Jian Fan's research while affiliated with Tongji Hospital and other places

... In recent years, aberrant expression of PAFAH1B3 has been detected as a metabolic oncogene in different kinds of tumors, including gastric cancer, 48 hypopharyngeal squamous cell carcinoma, 49 and osteosarcoma. 50 When analyzed using pan-cancer TCGA data, upregulated PAFAH1B3 was strongly associated with poor prognosis in a variety of tumors, including HCC. 51 Consistent with previous studies, we found that PAFAH1B3 expression levels were significantly higher in HCC tissues compared to normal tissues and were independently associated with patient prognosis, and that reducing PAFAH1B3 with siRNA significantly inhibited the proliferative and migratory properties of Hep3B cells. All these results suggested that PAFAH1B3 could be utilized as a biomarker for HCC prognosis. ...

... A previous report indicated that miR-204 inhibits the osteogenesis and promotes adipogenesis of mesenchymal stem cells by targeting Runx2 [48]. In the regulatory aspect of osteogenesis and chondrogenesis, RUNX2 has been regarded as a pivotal transcription factor [49]. Circ_2929 and circ_ARHGAP35-miR-204-5p-Runx2 may be potential axial regulators of osteoblast differentiation ( Figure 2C). ...

... Dex is a synthetic GC that has been widely used to treat inflammatory and autoimmune diseases. However, clinical evidence shows that the prolonged treatment with Dex causes growth retardation in treated children by triggering chondrocyte apoptosis within the growth plate [19,20]. In this study, we found that KLF2 expression was increased in chondrocytes of the tibial growth plate in Dex-treated rats. ...

... Real-Time qPCR remains the gold standard test for COVID-19 molecular diagnosis by detecting SARS-CoV-2 RNA and the NPS is the recommended sample [24][25][26]. However, our current finding showed higher detection and positivity rate in saliva for SARS-CoV-2 detection compared to NPS and this implies that saliva sample could serve as a good alternative for SARS-CoV-2 molecular diagnosis. ...

... А В С who had found that chemotherapy with methotrexate inhibited bone formation by inhibiting the activation of Wnt/β-catenin signaling pathway. As a result, the differentiation of osteoblastic cells providing mineralization of the bone matrix [13,14,15] was inhibited. King (2012) also found that methotrexate treatment promoted the formation of osteoclasts in the long bones of rats by increasing the level of proinflammatory cytokines and enhancing the activation of NF-kβ transcription factor. ...

... The measurement processes were conducted with an automatic analyzer Caris 200 (Xiamen UMIC Medical Instrument Co. Ltd., Xiamen, China). A double-antigen sandwich immunoassay was used to detect total antibody and a m-chain capture immunoassay was used to detect IgM antibody as previously reported (22). The total antibody and IgM antibody assays were established using recombinant antigens containing the RBD of the SARS-CoV-2 S protein (22). ...

... The outbreak of COVID-19 in December 2019 created disasters in almost every country and compelled countries to undertake drastic measures ranging from complete lockdowns, identification of effective therapies, development of vaccinesto the identification of risk factors for increased susceptibility and outcome of diseases in COVID-19 patients [23]. Many independent risk factors have been identified for the grave prognosis of COVID-19, which includes old age, cardiovascular diseases, diabetes mellitus, hypertension and chronic lung diseases [24]. ...

... However, there are currently few criteria for the 3D standard morphology of the distal radius. To date, studies on the 3D morphology of the distal radius have been performed using local morphological and morphometric evaluations based on CT images [10,11]. Although the methods used in a 3D analysis of distal radius fractures have undergone extensive development, the reference points that serve as criteria for reductions in the treatment of fractures have not yet been defined. ...

... Another point is the rating of people who had an asymptomatic infection-28.4%. Determining baseline antibodies prior to vaccination and then allocating patients to one of the groups is optimal [3,15]. An additional positive finding from the study is that no side effects are to be expected after vaccination, as none of the subjects had serious adverse events, which would not be expected in patients with COVID-19. ...

... Another evidence suggests that vertical transmission in the uterus is responsible for COVID-19 in neonates that makes neonatal infection through the umbilical cord unlikely. [18][19][20] Further studies are needed to determine whether COVID-19 antibodies protect neonates from COVID-19 infection; the concentration of IgG that can provide prevention; and whether the transplacental kinetics of vaccine-derived antibodies are similar to those obtained naturally. In addition, the results of this study were expected to be useful in determining the management of neonates from mothers infected with COVID-19. ...