Jehan Mansour’s research while affiliated with Zagazig University and other places

What is this page?


This page lists works of an author who doesn't have a ResearchGate profile or hasn't added the works to their profile yet. It is automatically generated from public (personal) data to further our legitimate goal of comprehensive and accurate scientific recordkeeping. If you are this author and want this page removed, please let us know.

Publications (1)


Production, Isolation, and Purification of L-Asparaginase from Pseudomonas Aeruginosa 50071 Using Solid-state Fermentation
  • Article

August 2004

·

411 Reads

·

268 Citations

Journal of Biochemistry and molecular biology

·

Mohamed Sarhan

·

Jehan Mansour

The L-asparaginase (E. C. 3. 5. 1. 1) enzyme was purified to homogeneity from Pseudomonas aeruginosa 50071 cells that were grown on solid-state fermentation. Different purification steps (including ammonium sulfate fractionation followed by separation on Sephadex G-100 gel filtration and CM-Sephadex C50) were applied to the crude culture filtrate to obtain a pure enzyme preparation. The enzyme was purified 106-fold and showed a final specific activity of 1900 IU/mg with a 43% yield. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the purified enzyme revealed it was one peptide chain with M(r) of 160 kDa. A Lineweaver-Burk analysis showed a K(m) value of 0.147 mM and V(max) of 35.7 IU. The enzyme showed maximum activity at pH 9 when incubated at 37 degrees C for 30 min. The amino acid composition of the purified enzyme was also determined.

Citations (1)


... ASP4, the method of solid state fermentation was carried out in the present study. Our study is in agreement with the contemporary studies which advocate the solid state fermentation as it comprises of slow growth of the mold and stable production of the enzyme (Dharmsthiti & Luechai, 2010;Basha et al., 2009;El-Naggar & El-Shweihy, 2020;El-Bessoumy et al., 2004;Siddalingeshwara & Lingappa, 2011;da Silva Menezes et al., 2017;Mohan Kumar & Manonmani, 2013). In contrast, various other studies have produced the enzyme by submerged fermentation (Sarquis et al., 2004;Basha et al., 2009;Amena et al., 2010;Vimal & Kumar, 2022;Chua et al., 2023;Baskar & Renganathan, 2009;Moharam et al., 2010;Moorthy et al., 2010). ...

Reference:

Production of L-Asparaginase using Soil Isolate of Cladosporium sp. and Evaluation of its Antitumor and Antioxidant Activities
Production, Isolation, and Purification of L-Asparaginase from Pseudomonas Aeruginosa 50071 Using Solid-state Fermentation
  • Citing Article
  • August 2004

Journal of Biochemistry and molecular biology