Jean Guilaine’s research while affiliated with Collège de France and other places

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Publications (458)


A ritual murder shaped the Early and Middle Neolithic across Central and Southern Europe
  • Article
  • Full-text available

April 2024

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273 Reads

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1 Citation

Science Advances

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Irina Tupikova

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[...]

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Eric Crubézy

In the Rhône Valley’s Middle Neolithic gathering site of Saint-Paul-Trois-Châteaux (France), the positioning of two females within a structure aligned with the solstices is atypical. Their placement (back and prone) under the overhang of a silo in front of a third in a central position suggests a ritualized form of homicidal ligature strangulation. The first occurrence dates back to the Mesolithic, and it is from the Early Neolithic of Central Europe that the practice expands, becoming a sacrificial rite associated with an agricultural context in the Middle Neolithic. Examining 20 cases from 14 sites spanning nearly two millennia from Eastern Europe to Catalonia reveals the evolution of this ritual murder practice.

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Genetic structure of the 317 herein-reported ancient genomes
a–d, PCA of 3,316 modern and ancient individuals from Eurasia, Oceania and the Americas (a,b), as well as restricted to 2,126 individuals from western Eurasia (west of the Urals) (c,d). Shown are analyses with principal components inferred either using both modern and imputed ancient genomes passing all filters, and projecting low coverage ancient genomes (a,c); or only modern genomes and projecting all ancient genomes (b,d). Ancient genomes sequenced in this study are indicated either with black circles (imputed genomes) or grey diamonds (projected genomes). e, Model-based clustering results using ADMIXTURE for 284 newly reported genomes (excluding close relatives and individuals flagged for possible contamination). Results shown are based on ADMIXTURE runs from K = 2 to K = 15 on 1,593 ancient individuals, corresponding to the full set of 1,492 imputed genomes passing filters as well as 101 low coverage genomes represented by pseudo-haploid genotypes (flags “lowcov” or “lowGpAvg”, Supplementary Data 7; indicated with alpha transparency in plot).
Imputation accuracy of ancient DNA
a, Imputation accuracy across 42 high-coverage ancient genomes when downsampled to lower depth of coverage values (see Supplementary Note 2 and Supplementary Table 2.1). b, Imputation accuracy for 1× depth of coverage across 9 prehistoric European genomes; c, across 5 Viking age genomes; and d, across 7 ancient genomes from Early Medieval Hungary. In all panels, imputation accuracy is shown as the squared Pearson correlation between imputed and true genotype dosages as a function of MAF of the target variant sites.
Genetic clustering of ancient individuals
Characterization of genetic clusters for 1,401 imputed ancient individuals from Eurasia (that is, excluding 91 individuals from Africa and Americas), inferred from pairwise IBD sharing (indicated using coloured symbols throughout), a, Temporal distribution of clustered individuals, grouped by broad ancestry cluster. b,c, Geographical distribution of clustered individuals, shown for individuals predating 3,000 bp (b) and after 3,000 bp (c). d, Network graph of pairwise IBD sharing between 596 ancient Eurasians predating 3,000 bp, highlighting within- and between-cluster relationships. Each node represents an individual, and the width of edges connecting nodes indicates the fraction of the genome shared IBD between the respective pair of individuals. Network edges were restricted to the 10 highest sharing connections for each individual, and the layout was computed using the force-directed Fruchterman-Reingold algorithm. e, Neighbour-joining tree showing relationships between genetic clusters, inferred using total variation distance (TVD) of IBD painting palettes. f,g, PCA of 3,119 Eurasian (f) or 2,126 west Eurasian (g) ancient and modern individuals (“HO” dataset).
Genetic structure of European HGs after the LGM
a, Supervised ancestry modelling using non-negative least squares on IBD sharing profiles. Panels show estimated ancestry proportions for target individuals from genetic clusters representing European HGs, using different sets of increasingly proximal source groups. Individuals used as sources in a particular set are indicated with black crosses and coloured bars with 100% ancestry proportion. Black lines indicate 1 standard error for the respective ancestry component. b, Residuals for model fit of target individuals from selected genetic clusters across different source sets. c, Moon charts showing spatial distribution of ancestry proportions in European HGs deriving from four European source groups (set “hgEur2”; source origins shown with coloured symbol). Estimated ancestry proportions are indicated by both size and amount of fill of moon symbols. Note that ‘Italy_15000BP_9000BP’ and ‘RussiaNW_11000BP_8000BP’ correspond to ‘WHG’ and ‘EHG’ labels used in previous studies. d, Maps showing networks of highest between-cluster IBD sharing (top 10 highest sharing per individual) for individuals from two genetic clusters representing Scandinavian HGs. See Supplementary Data 1 and 7 for details of individual sample IDs presented here.
Ancestry modelling for HG and Neolithic farmer-associated genetic clusters
Supervised ancestry modelling using non-negative least squares on IBD sharing profiles. Panels show estimated ancestry proportions of two global Eurasian clusters, corresponding to European HGs before 4,000 bp and individuals from Europe and western Asia from around 10,000 bp until historical times, including Anatolian-associated (Neolithic) farmers, Caucasus HGs and recent individuals with genetic affinity to the Levant. Columns show results of modelling target individuals using three panels of increasingly distal source groups: “postBA”: Bronze Age and Neolithic source groups; “postNeol”, Bronze Age and later targets using Late Neolithic/early Bronze Age and earlier source groups; “deep”, Mesolithic and later targets using deep ancestry source groups. Individuals used as sources in a particular set are indicated with black crosses and coloured bars with 100% ancestry proportion. Black lines indicate 1 standard error for the respective ancestry component.

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Population genomics of post-glacial western Eurasia

January 2024

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2,010 Reads

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53 Citations

Nature

Western Eurasia witnessed several large-scale human migrations during the Holocene1–5. Here, to investigate the cross-continental effects of these migrations, we shotgun-sequenced 317 genomes—mainly from the Mesolithic and Neolithic periods—from across northern and western Eurasia. These were imputed alongside published data to obtain diploid genotypes from more than 1,600 ancient humans. Our analyses revealed a ‘great divide’ genomic boundary extending from the Black Sea to the Baltic. Mesolithic hunter-gatherers were highly genetically differentiated east and west of this zone, and the effect of the neolithization was equally disparate. Large-scale ancestry shifts occurred in the west as farming was introduced, including near-total replacement of hunter-gatherers in many areas, whereas no substantial ancestry shifts happened east of the zone during the same period. Similarly, relatedness decreased in the west from the Neolithic transition onwards, whereas, east of the Urals, relatedness remained high until around 4,000 bp, consistent with the persistence of localized groups of hunter-gatherers. The boundary dissolved when Yamnaya-related ancestry spread across western Eurasia around 5,000 bp, resulting in a second major turnover that reached most parts of Europe within a 1,000-year span. The genetic origin and fate of the Yamnaya have remained elusive, but we show that hunter-gatherers from the Middle Don region contributed ancestry to them. Yamnaya groups later admixed with individuals associated with the Globular Amphora culture before expanding into Europe. Similar turnovers occurred in western Siberia, where we report new genomic data from a ‘Neolithic steppe’ cline spanning the Siberian forest steppe to Lake Baikal. These prehistoric migrations had profound and lasting effects on the genetic diversity of Eurasian populations.


The southeast area of the middle terrace (Central and B Sectors)

January 2024

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1 Read

Klimonas is the oldest Mediterranean island village. Occupied ca. 8 800 cal BC, it postpones by several centuries the Neolithic presence in Cyprus, at that time located more than 80 km offshore. The village extended over more than 5,500 m2, facing the sea, 2 km from the famous pre-pottery site of Shillourokambos and near rich flint outcrops. Excavations (2009-2016) revealed that it was composed of circular or oval earthen buildings 3-6 m in diameter, notched into the slope, modestly fitted out and organised around a semi-buried 10 m communal building. The construction techniques, the abundance of either knapped or polished stone material, together with ornaments, symbolic objects, and plants and animal remains, as well as the 52 radiometric dates, point to the end of the Levantine Pre-Pottery Neolithic A (PPNA). The presence of a communal building, rebuilt numerous times over the course of several decades, also points to the same conclusion. The villagers gathered seeds and fruits and cultivated wild starch and einkorn, recently imported from the continent. They primarily hunted small endemic wild boar, the only large mammal species attested on the island at that time and, secondarily, birds. They did not eat fish or marine shellfish. Domestic dogs, mice and cats brought from the continent also lived in the village. The remains of this cultivator-hunter community testify to the early extension of the Near Eastern Neolithic and to unsuspected seafaring skills, substantially improving our knowledge of the Neolithic transition in the Mediterranean.


Klimonas et le PPNA continental : éléments d’une problématique

January 2024

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2 Reads

Klimonas is the oldest Mediterranean island village. Occupied ca. 8 800 cal BC, it postpones by several centuries the Neolithic presence in Cyprus, at that time located more than 80 km offshore. The village extended over more than 5,500 m2, facing the sea, 2 km from the famous pre-pottery site of Shillourokambos and near rich flint outcrops. Excavations (2009-2016) revealed that it was composed of circular or oval earthen buildings 3-6 m in diameter, notched into the slope, modestly fitted out and organised around a semi-buried 10 m communal building. The construction techniques, the abundance of either knapped or polished stone material, together with ornaments, symbolic objects, and plants and animal remains, as well as the 52 radiometric dates, point to the end of the Levantine Pre-Pottery Neolithic A (PPNA). The presence of a communal building, rebuilt numerous times over the course of several decades, also points to the same conclusion. The villagers gathered seeds and fruits and cultivated wild starch and einkorn, recently imported from the continent. They primarily hunted small endemic wild boar, the only large mammal species attested on the island at that time and, secondarily, birds. They did not eat fish or marine shellfish. Domestic dogs, mice and cats brought from the continent also lived in the village. The remains of this cultivator-hunter community testify to the early extension of the Near Eastern Neolithic and to unsuspected seafaring skills, substantially improving our knowledge of the Neolithic transition in the Mediterranean.


Location, excavation, nomenclature

January 2024

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9 Reads

Klimonas is the oldest Mediterranean island village. Occupied ca. 8 800 cal BC, it postpones by several centuries the Neolithic presence in Cyprus, at that time located more than 80 km offshore. The village extended over more than 5,500 m2, facing the sea, 2 km from the famous pre-pottery site of Shillourokambos and near rich flint outcrops. Excavations (2009-2016) revealed that it was composed of circular or oval earthen buildings 3-6 m in diameter, notched into the slope, modestly fitted out and organised around a semi-buried 10 m communal building. The construction techniques, the abundance of either knapped or polished stone material, together with ornaments, symbolic objects, and plants and animal remains, as well as the 52 radiometric dates, point to the end of the Levantine Pre-Pottery Neolithic A (PPNA). The presence of a communal building, rebuilt numerous times over the course of several decades, also points to the same conclusion. The villagers gathered seeds and fruits and cultivated wild starch and einkorn, recently imported from the continent. They primarily hunted small endemic wild boar, the only large mammal species attested on the island at that time and, secondarily, birds. They did not eat fish or marine shellfish. Domestic dogs, mice and cats brought from the continent also lived in the village. The remains of this cultivator-hunter community testify to the early extension of the Near Eastern Neolithic and to unsuspected seafaring skills, substantially improving our knowledge of the Neolithic transition in the Mediterranean.



Klimonas and the continental PPNA: small pieces of a larger issue

January 2024

Klimonas is the oldest Mediterranean island village. Occupied ca. 8 800 cal BC, it postpones by several centuries the Neolithic presence in Cyprus, at that time located more than 80 km offshore. The village extended over more than 5,500 m2, facing the sea, 2 km from the famous pre-pottery site of Shillourokambos and near rich flint outcrops. Excavations (2009-2016) revealed that it was composed of circular or oval earthen buildings 3-6 m in diameter, notched into the slope, modestly fitted out and organised around a semi-buried 10 m communal building. The construction techniques, the abundance of either knapped or polished stone material, together with ornaments, symbolic objects, and plants and animal remains, as well as the 52 radiometric dates, point to the end of the Levantine Pre-Pottery Neolithic A (PPNA). The presence of a communal building, rebuilt numerous times over the course of several decades, also points to the same conclusion. The villagers gathered seeds and fruits and cultivated wild starch and einkorn, recently imported from the continent. They primarily hunted small endemic wild boar, the only large mammal species attested on the island at that time and, secondarily, birds. They did not eat fish or marine shellfish. Domestic dogs, mice and cats brought from the continent also lived in the village. The remains of this cultivator-hunter community testify to the early extension of the Near Eastern Neolithic and to unsuspected seafaring skills, substantially improving our knowledge of the Neolithic transition in the Mediterranean.


The Neolithic village of Klimonas: technical, social and symbolic aspects; insularity and connectivity

January 2024

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86 Reads

Klimonas is the oldest Mediterranean island village. Occupied ca. 8 800 cal BC, it postpones by several centuries the Neolithic presence in Cyprus, at that time located more than 80 km offshore. The village extended over more than 5,500 m2, facing the sea, 2 km from the famous pre-pottery site of Shillourokambos and near rich flint outcrops. Excavations (2009-2016) revealed that it was composed of circular or oval earthen buildings 3-6 m in diameter, notched into the slope, modestly fitted out and organised around a semi-buried 10 m communal building. The construction techniques, the abundance of either knapped or polished stone material, together with ornaments, symbolic objects, and plants and animal remains, as well as the 52 radiometric dates, point to the end of the Levantine Pre-Pottery Neolithic A (PPNA). The presence of a communal building, rebuilt numerous times over the course of several decades, also points to the same conclusion. The villagers gathered seeds and fruits and cultivated wild starch and einkorn, recently imported from the continent. They primarily hunted small endemic wild boar, the only large mammal species attested on the island at that time and, secondarily, birds. They did not eat fish or marine shellfish. Domestic dogs, mice and cats brought from the continent also lived in the village. The remains of this cultivator-hunter community testify to the early extension of the Near Eastern Neolithic and to unsuspected seafaring skills, substantially improving our knowledge of the Neolithic transition in the Mediterranean.


Central Sector: Communal building and its immediate surroundings

January 2024

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3 Reads

Klimonas is the oldest Mediterranean island village. Occupied ca. 8 800 cal BC, it postpones by several centuries the Neolithic presence in Cyprus, at that time located more than 80 km offshore. The village extended over more than 5,500 m2, facing the sea, 2 km from the famous pre-pottery site of Shillourokambos and near rich flint outcrops. Excavations (2009-2016) revealed that it was composed of circular or oval earthen buildings 3-6 m in diameter, notched into the slope, modestly fitted out and organised around a semi-buried 10 m communal building. The construction techniques, the abundance of either knapped or polished stone material, together with ornaments, symbolic objects, and plants and animal remains, as well as the 52 radiometric dates, point to the end of the Levantine Pre-Pottery Neolithic A (PPNA). The presence of a communal building, rebuilt numerous times over the course of several decades, also points to the same conclusion. The villagers gathered seeds and fruits and cultivated wild starch and einkorn, recently imported from the continent. They primarily hunted small endemic wild boar, the only large mammal species attested on the island at that time and, secondarily, birds. They did not eat fish or marine shellfish. Domestic dogs, mice and cats brought from the continent also lived in the village. The remains of this cultivator-hunter community testify to the early extension of the Near Eastern Neolithic and to unsuspected seafaring skills, substantially improving our knowledge of the Neolithic transition in the Mediterranean.


Citations (23)


... As at W-80, primary and perpendicular axes are integral to the spatial organization of this reconstruction, with a substantial hearth lyinglike feature [136] directly upon the primary axis in the open space to the front. Building 800 at the early-9th-millennium, PPNA-related site of Klimonas on the island of Cyprus is another credible early comparandum (Vigne et al. 2023b). ...

Reference:

Not a place for respectable people, but the ends of the earth converge there: insights from Wisad Pools into the nature and context of Jordan’s Black Desert Neolithic
Sector F, building 800 and other evidence of occupation on the upper terrace
  • Citing Chapter
  • January 2024

... As a material, earth can be extremely technical to excavate; it tends to produce shapeless-seeming ruins unless it has been subject to the right taphonomical conditions (Claasz Coockson 2010;Friesem et al. 2011). PPNA sites can be reduced to very scarce remains when exposed to a more humid Mediterranean climate (see figure 2c) or when they are not occupied long enough to generate a protective layer of debris (Vigne et al. 2023). But while this bias, and the way it affects the representativity of remains, is now increasingly recognized, another more theoretical dimension of it is still prevalent in our general way of appreciating the site formation process. ...

The Neolithic village of Klimonas: technical, social and symbolic aspects; insularity and connectivity/Le village néolithique de Klimonas. Aspects techniques, sociaux et symboliques ; insularité et connectivité
  • Citing Chapter
  • January 2023

... There is always an amount of tension between functionalist and ritual interpretations, but it is notable that the criteria for making these determinations sometimes vary greatly between human and faunal studies. The criteria for recognising sacrifice for instance: in human remains there is a focus around the idea of "overkill" or excessive brutality and violence at time of death (Aldhouse-Green 2001; Schwarz, 2018;Ludes et al. 2024), while in faunal material articulation (Morris 2010, Grant 1984, demographic patterns (Grimm andWorley 2011, Groot 2012), and the presence of human remains within the same feature (Morris 2010, Sykes 2015, Wait 1985 have all been used to identify probable sacrificed animals and violence is rarely considered. ...

A ritual murder shaped the Early and Middle Neolithic across Central and Southern Europe

Science Advances

... If initially we were talking about two such components, the so-called "Eastern Hunter-Gatherers", EHG (that is, ultimately ANE, "Ancient North Eurasians") and "Caucasian Hunter-Gatherers", CHG ("population mixture, specifically EHG and CHG/ Iranian ancestry, a combination that forms the so-called 'steppe-ancestry'" 81 ), now a third one is added to them, and added in even decisive roles -"Western Hunter-Gatherers", WHG. 82 This fact, by the way, has not yet been clearly recognized by either Lazaridis et al. 2024 or their competitors Allentoft et al. 2024. Meanwhile, it is fundamentally important. ...

Population genomics of post-glacial western Eurasia

Nature

... This is particularly noteworthy, considering that even anciently introduced anthropochorous species are considered of conservation value as part of our "cultural heritage" (Masseti 2009). The success of M. cypriacus in persisting despite the range of habitat modifications (e.g., urbanization and the introduction of non-native species like cats and rats, to name only a few) that led to the extinction of all other endemic rodents on Mediterranean islands might be attributed to its evolution in a context that involved terrestrial predation by a local, endemic predator, the Cypriot genet Genetta plesictoides (Masseti 2009;Vigne et al. 2023). As a result, M. cypriacus was not naïve towards introduced terrestrial predators, showcasing a resilience that sets it apart. ...

2023-Chypre-JASR: Historical dynamics of the human-environment interactions in Cyprus during the 12th-10th millennia cal. BP: The last 30 years contributions of the Amathous area (Limassol district)
  • Citing Article
  • June 2023

Journal of Archaeological Science Reports

... Pigs have a long history of association with humans and were domesticated between 9500 and 8000 BCE in the Near-East (Price & Hongo, 2019). These pigs arrived in Western Europe around 5400 BCE and hybridized with European wild boars (see overview in Cucchi et al., 2023). In mammals, changes in feeding behavior in captivity often induce functional responses resulting in shape differences in the skull and mandible (Hartstone-Rose et al., 2014). ...

4500 years of morphological diversification in Western Europe wild boars (Sus scrofa) and the consequences of the Neolithic transition
  • Citing Article
  • May 2023

Quaternary Science Reviews

... In recent years, however, the long-standing debates about culture historical entities have been revived and given a new significance by the results of the whole genome aDNA studies of prehistoric populations that have appeared, many of them focused on populations of the last ten thousand years in Europe that have been central to culture historical studies (e.g. Allentoft et al. 2015;Haak et al. 2015;Hofmanová et al. 2016;Lazaridis et al. 2016). The reason for the controversy is that the findings seem to support some of the classic culture history claims that archaeological cultures correspond to biological populations (Frieman & Hofmann 2019;Furholt 2018;Kristiansen 2022). ...

Population Genomics of Stone Age Eurasia

... For example, the dichotomy between megalithic monuments and cavities gave rise to debates surrounding the reasons for these distinct choices in funerary locations. Since the natural settings of certain regions within southern France permit both types of structures to coexist, the choice of location calls for arguments other than environmental opportunism and could be connected to cultural preferences and traditions [1][2][3][4]. Frequently used over several generations, collective burials are generally seen to include individuals from the same ...

Mégalithes et grottes funéraires: Cohabitation ? Complémentarité ? Exclusion ? Une histoire complexe
  • Citing Article
  • December 2021

Préhistoires méditerranéennes

... For each target, in the legend, we show the inferred average dates of admixture (±1 SE) in generations before the individual lived, in BCE accounting for the average age of all the individuals and the mean human generation time, and the normalized root-mean-square deviation (NRMSD) values to assess the fit of the exponential curve (Materials and methods). The bottom left shows the ancestry covariance decay curve for early Anatolian Earlier analysis has suggested that farming spread along two main routes in Europe, from southeast to central Europe ('continental route') and along the Mediterranean coastline to Iberia ('coastal route') (Gronenborn, 2014;Guilaine, 2003;Rivollat et al., 2020). Consistent with this, we inferred one of the earliest timings of gene flow was in the Balkans around 6400 BCE. ...

Du Levant à l’Espagne : aspects de la Néolithisation en Méditerranée
  • Citing Article
  • January 2020

... Binder et Perlès, 1990 ;Pétrequin et al., 2012), rôle de support ou de vecteur des expressions graphiques (par ex. Larocca, 2005 ;Martí Oliver et al., 2018 ;Lopez-Montalvo et al., 2021). ...

Figurative graphic expressions and the first Western Mediterranean farmers: A new zoomorphic contribution from Southern France
  • Citing Article
  • September 2021

Bulletin de la Société préhistorique française