Janet A.W. Elliott’s research while affiliated with University of Alberta and other places

... Among these, the GelRed was one of the most sensitive and safer dyes, which is more and more widely used in gel electrophoresis for visualizing DNA [27,28]. Recently, it has been found that GelRed could selectively permeate into dead cells and has the potential to be employed for bacteria and mammalian cell viability assays [29][30][31]. In the present study, we optimized the protocols of GelRed staining for mammalian cells. ...

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Cell viability assessment by using GelRed/SYTO 9-based double staining

... All living cells, including the spermatozoa, are in an isotonic state at room temperature in order to maintain its physiological volume constant [9], but when the temperature drops below − 10 • C, the first ice nuclei nascent predominantly in the extracellular water (due to the protective role of cell membrane), altering the solution-to-solute ratio on both sides of the cell membrane [10]. From this point on, two oppositely dependent on the cooling rate processes determine the low temperature response of the biological system [11,5,[12][13][14]10,15,16]. If the freezing proceeds sufficiently slow, the extracellular solutes (salts) relocate in the dwindling unfrozen liquid and trigger osmotic elimination of the intracellular water, because the chemical equilibrium has to be restored [10]. ...

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Probing the impact of commercial cryoprotectants and freezing technique on the motility of human spermatozoa cryopreserved onto extremely water-repellent soot-coated surfaces

... Recent studies in the field of regenerative medicine have demonstrated the great potential of MSCs in treating various diseases [23][24][25] . MSCs are already being developed as cell products for immunotherapy and regenerative medicine 26,27 . ...

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Long-term cryopreserved umbilical cord derived mesenchymal stem cells: A study on their safety

... We note that, however, we have applied an extension of this model to dissociating solutes in another paper. 28 There, the predictions of the present model for freezing points of 11 ternary inorganic salt solutions of water were found to be in excellent agreement with independent experimental measurements (root-mean square error of 0.45 K and close-to-zero mean bias for a total of 371 data points). ...

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Multicomponent solutions: Combining rules for multisolute osmotic virial coefficients

... Recently, 31 we further explored the theoretical aspects of the MSOVE and developed generalized combining rules for coefficients of arbitrary order in a specific solution theory framework. All newly derived combining rules take the form of an arithmetic-average of suitable pure-term coefficients, which contrasts with the geometricaverage combining rule used for the cubic terms in the E-MSOVE implementation. ...

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Predicting freezing points of ternary salt solutions with the multisolute osmotic virial equation

... Among these, the GelRed was one of the most sensitive and safer dyes, which is more and more widely used in gel electrophoresis for visualizing DNA [27,28]. Recently, it has been found that GelRed could selectively permeate into dead cells and has the potential to be employed for bacteria and mammalian cell viability assays [29][30][31]. In the present study, we optimized the protocols of GelRed staining for mammalian cells. ...

Reference:

Cell viability assessment by using GelRed/SYTO 9-based double staining

... A fundamental thermodynamic question is addressed in Ref. 5. They address the relation between vapor of a liquid, a finite contact angle, and a possible wetting transition at saturation vapor pressure. ...

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Chemical physics of controlled wettability and super surfaces

... Hindering the gas solubility in the liquid phase [88] and the time-dependent gas diffusion [68,107] in the nanopores has proven effective in enhancing material reusability. Moreover, researchers [104,108,109] have extensively studied the interplay between the liquid-solid interaction and the liquid-gas interaction to better regulate liquid outflow behavior and improve the [91]. (d) Stress-strain curves of water-ZIF LN at three different strain rates using SHPB [73]. ...

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Recent Experimental Advances in Solid–Liquid Composites for Impact and Blast Mitigation

... The accommodation coefficient, which is believed to be a temperature-dependent quantity [36,37] and highly sensitive to impurities at the liquid-vapor interface and the presence of non-condensable gases in the vapor [34], is used to calculate the evaporation and condensation mass fluxes by the Schrage equation [38]. Determining the accommodation coefficients and understanding the interfacial phenomena during phase change processes remain active areas of research [39][40][41][42][43][44][45]. Consequently, the accommodation coefficient is often treated as a fitting parameter dependent on the working fluid and the operating conditions [46][47][48]. ...

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Advancing thermohydraulic performance of micro-grooved flat heat pipes through a combined numerical-experimental approach

... Examples of such cocktails include improved VS41A, Natural Deep Eutectic Systems (NADES), and others used for vitrification of biological samples (88)(89)(90)(91). Notably, mathematical models have been used in the development of low-toxicity vitrification solutions (66,92,93), a form similar to computer simulation of drug development or a trend in the development of new CPAs. And the method of hypothermic machine perfusion with multiple steps of loading/unloading CPAs can reduce the toxicity and osmotic damage to organs (94). ...

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Cryopreservation of tissues and organs: present, bottlenecks, and future