Jan W. P. Kuiper's research while affiliated with Radboud University and other places

Publications (24)

Article
Full-text available
Directional cell migration is an essential requirement for efficient neutrophil translocation to sites of infection and requires the establishment of a polarized cell characterized by an actin-rich leading edge facing the chemoattractant gradient. The asymmetrical accumulation of phosphatidylinositol(3,4,5)-trisphosphate [PtdIns(3,4,5)P(3)] in the...
Article
Neutrophils play a critical role as a first line of defense against invading pathogens. Recently, a new defense strategy of neutrophils was described, in which pathogens are trapped and killed by NETs. However, the exact underlying mechanisms leading to the formation of NETs remain elusive. Here, we explored the role of the Rac small GTPases in the...
Article
Full-text available
It is generally accepted that hydrogenosomes (hydrogen-producing organelles) evolved from a mitochondrial ancestor. However, until recently, only indirect evidence for this hypothesis was available. Here, we present the almost complete genome of the hydrogen-producing mitochondrion of the anaerobic ciliate Nyctotherus ovalis and show that, except f...
Article
Full-text available
Hydrogenosomes are organelles that produce molecular hydrogen and ATP. The broad phylogenetic distribution of their hosts suggests that the hydrogenosomes of these organisms evolved several times independently from the mitochondria of aerobic progenitors. Morphology and 18S rRNA phylogeny suggest that the microaerophilic amoeboflagellate Psalteriom...
Article
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There are thousands of very diverse ciliate species from which only a handful mitochondrial genomes have been studied so far. These genomes are rather similar because the ciliates analysed (Tetrahymena spp. and Paramecium aurelia) are closely related. Here we study the mitochondrial genomes of the hypotrichous ciliates Euplotes minuta and Euplotes...
Data
Morphology measurements of migrating MEFs. In the top panel, the analysis of migration morphology is illustrated. The number of lamellipodia per cell, the lamellipodium dimensions (width and length measured with cell body as reference, lamellipodium includes lamella-region) and tail length was measured. Lower panels show high magnification images o...
Data
CK-B mediates astrocyte migration. WT (upper panels) and CK-B−/− (lower panels) astrocytes migrating along laminin for 48 h. Migration assays were performed without (left panels) and with (right panels) cyclocreatine treatment. Both CK-B knockout and inhibition strongly decreased migratory behavior of astrocytes. Video S1 corresponds with Figure 4A...
Data
Cyclocreatine does not alter proliferation and apoptosis. A) Proliferation of MEF-CK-B (left) and MEF-MYR-CK-B (right) cells with (red) and without (black) cCr (5 mM) treatment. Relative proliferation rates, taking non-treated cells as control, are shown of three independent experiments. (B) Apoptosis of MEF-CK-B and MEF-MYR-CK-B cells cultured wit...
Data
Repositioning of CK-B to cellular membranes induces migration. MEF-MYR-FKBP cells transfected with FRB-CK-B (upper panels) or FRB-CK-BC283s (lower panels) were allowed to migrate along FN without (left panels) and with Rapalog (right panels). Rapalog-mediated targeting of CK-B to membranes, but not CK-BC283s membrane targeting, stimulated migration...
Data
Rapalog-induced membrane localization of CK-B and CK-BC238S in MEFs. (A) MEF-BAK−/− cells stably expressing MYR-FKBP were retrovirally transduced with FRB-CK-B (upper panels) or CK-BC283S (lower panels) and stained for CK-B. Rapalog treatment (100 nM, 1 h, left panels) resulted in translocation of (a fraction of) CK-B and CK-BC283S to cellular memb...
Data
MEF-MYR-CK-B cells spread out faster than MEF-CK-B cells. Quantification of MEF spreading on FN for 30 min, showing that expression of MYR-CK-B facilitates cell spreading. * p<0.05. (0.68 MB TIF)
Data
Cyclocreatine selectively inhibits CK-B-induced migration. MEF-YFP, MEF-CK-B, MEF-MYR-CK-B and MEK-AK1 cells were followed during migration along FN. In contrast to YFP and AK1 complemented MEFs, MEF-CK-B and MEF-MYR-CK-B migration was inhibited by cCr and a strong morphological effect was observed. Video S3 corresponds with Figure 6C. Time-lapse p...
Data
CK-B expression and localization affects MEF migration. YFP/MYR-YFP complemented (upper panels) and CK-B/MYR-CK-B complemented MEFs migration along FN for 24 h. Migration assays of non-targeted (left panels) and membrane-targeted (right panels) cells showed that CK-B induced migration and additionally that MEF-MYR-CK-B were the most motile. Video S...
Article
Full-text available
Creatine Kinases (CK) catalyze the reversible transfer of high-energy phosphate groups between ATP and phosphocreatine, thereby playing a storage and distribution role in cellular energetics. Brain-type CK (CK-B) deficiency is coupled to loss of function in neural cell circuits, altered bone-remodeling by osteoclasts and complement-mediated phagocy...
Article
Full-text available
Author Summary To do work, cells need energy in the form of ATP. High and sudden energy demand is seen during cell-shape change, a process in which ATP fuels the cytoskeletal machinery that drives cell-morphology alteration. How a cell organizes high-energy surges without disrupting global ATP homeostasis remains an important research question. On...
Data
Movie of a EYFP-CK-B–Transfected Cell Phagocytozing Zymosan RAW 264.7 cells were transiently transfected with pEYFP-CK-B. Texas Red–labeled zymosan particles were added to the cells, and time-lapse microscopy was performed with an interval of 3 s between subsequent frames. (1.2 MB MOV)
Data
Determination of the Mobility of CK-B and Actin in the Phagocytic Cup (A) Average (n = 14) of normalized bleach and recovery curves of RAW264.7 cells stably expressing ECFP-CK-B and EYFP-actin. Fluorescence of CFP and YFP in the phagocytic cup was bleached by scanning a square region covering approximately half the cup area 20 times at high laser p...
Data
Phagocytosis of Complement- and IgG-Coated Polystyrene Beads Uptake capacity for fluorescent polystyrene beads in RAW 264.7 cells preincubated with 5 mM creatine, 5 mM cyclocreatine, or normal growth medium was quantified by FACS. (A) Complement-coated 3-μm polystyrene beads. (B) IgG-coated 3-μm polystyrene beads. Bars represent averages of Fl-1–po...
Data
Detailed Description of All Procedures (55 lB DOC)
Data
Determination of Receptors Used in Uptake of Phagocytic Particles The receptors on RAW 264.7 cells involved in binding of differentially opsonized zymosan particles were determined in the presence of a CR3 blocking monoclonal antibody (M1/70, anti-CD11b) (A and B) or human IgG (3 mg/ml) (C and D). Bar diagrams represent the percentage particles (in...
Article
Full-text available
Neurons require an elaborate system of intracellular transport to distribute cargo throughout axonal and dendritic projections. Active anterograde and retrograde transport of mitochondria serves in local energy distribution, but at the same time also requires input of ATP. Here we studied whether brain-type creatine kinase (CK-B), a key enzyme for...
Article
Maintenance of a tight coupling between energy production and consumption of energy-metabolites in different cellular locales, i.e., energy homeostasis regulation, is crucial for proper functioning and viability control of every cell. Balance in global and local concentrations of ATP, the key energy metabolite of life, is hereto essential. A better...
Article
Full-text available
Adenylate kinases (AK, EC 2.7.4.3) have been considered important enzymes for energy homeostasis and metabolic signaling. To gain a better understanding of their cell-specific significance we studied the structural and functional aspects of products of one adenylate kinase gene, AK1, in mouse tissues. By combined computer database comparison and No...

Citations

... The generation of CK-B knockout mice and the study of genotype-phenotype relationships of these animals in comparison to wildtype controls has been described in detail elsewhere (also [17,57]). Primary cultures of mouse hippocampal neurons were established using a modified protocol [58]. ...
... Cell morphology is based on the dynamics of the cytoskeleton, including polymerization and depolymerization of stress fibers regulated by the balance between RhoA-and Rac-1-mediated signaling. 9,10 Reactive oxygen species (ROS) produced by mitochondrial activation disrupts the balance between stress fiber polymerization and depolymerization, 11 yielding spindle-shaped cells due to inhibition of stress fiber disassembly. This provides a rationale for measuring the balance of the redox system and mitochondrial activity as the PPs that underlie the formation of pseudopods in late passages. ...
... Rac proteins are expressed in neutrophils as three highly homologous proteins (Rac1, Rac2, and Rac3) (Dinauer, 2003). Our results aligned with Rac2 in NETs (Lim et al., 2011), indicating that Rac1 and Rac2 performed overlapping functions in NETosis. ...
... Nyctotherus ovalis has a hydrogen-producing mitochondrion [5]. The amoebozoan Entamoeba istolytica contains mitosomes, which have lost the ability to produce ATP [6]. ...
... It seems that anaerobic lineages arose at least three times in Heterolobosea. Psalteriomonadidae, Creneis and Dactylomonas represent distinct lineages that possess mitochondrion-related organelles that lack cristae and are known or suspected to function anaerobically, for example as hydrogenosomes (Park et al. 2007, de Graaf et al. 2009, Barberà et al. 2010, Hanousková et al. 2019. It is suggested that the lack of cristae in anaerobic protists such as psalteriomonads is related to a loss of the mitochondrial contact site and cristae organizing system (MICOS) (see fig. 5 in Muñoz-Gómez et al. 2015b), localized in the cristae junctions. ...
... www.nature.com/scientificreports/ and brown algae (Heteroconta), cox2 is split by insertions that are thought to be translated in-frame with flanking cox2 fragments into a single enlarged COXII polypeptide (e.g., Ref. [23][24][25]. ...
... CK comprises two cytosolic isoenzymes (CK-brain type and CKmuscle type) and two mitochondrial isoenzymes (Wyss and Wallimann 1994). Since CK is responsible for energy homeostasis, it participates in many biological and physiological events (Shin et al. 2007;Salin-Cantegrel et al. 2008;Kuiper et al. 2009). The brain type of creatine kinase (CKB) is upregulated in several cancer cells, including breast cancer cells. ...
... Any restricted ADP rephosphorylation will lower the free energy of the ATP/ ADP pool, ΔG ATP , potentially restricting multiple ATP-dependent processes and activating AMP-dependent protein kinase 47 . This is apparent not only in muscle, but also in brain, where the pathway is important for the function of the actin cytoskeleton and cell and mitochondrial motility 48 . Thus, germline deletion of the cytosolic brain-type creatine kinase isoform results in defective thermoregulation and severe hypothermia owing to impaired neuronal function rather than any defect in BAT 49 . ...
... Two A ↔ T-transformed swinger RNAs detected in the colon and head-neck cancer lines mapped on the adenylate kinase 1 (AK1) coding gene ( Table 2). AK1 plays an important role in tumor suppression and it is often downregulated in cancer cells (Collavin et al., 1999;Janssen et al., 2004;Vasseur et al., 2005;Jan et al., 2019). Three swinger RNAs detected in breast (A ! ...
... Microglial phagocytosis is associated with inflammatory responses of microglia and neuron or photoreceptors loss under chronic neurodegenerative conditions, such as retinitis pigmentosa (Zhao et al., 2015). Phagocytosis requires locally coordinated complex cytoskeletal rearrangements (Kuiper et al., 2008); we, therefore, evaluated the phagocytotic activities of BV-2 cells with or without ES treatment by incubating the cultures with fluorescent zymosan particles. Quantification at 24 h post-ES revealed a significant reduction in the total number of fluorescent zymosan particles and zymosan particles per cell ( Figure 3C Frontiers in Cell and Developmental Biology frontiersin.org ...