Jan M. van der Wolf's research while affiliated with Wageningen University & Research and other places

Publications (33)

Article
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Irrigation with surface water carrying plant pathogens poses a risk for agriculture. Managed aquifer recharge enhances fresh water availability while simultaneously it may reduce the risk of plant diseases by removal of pathogens during aquifer passage. We compared the transport of three plant pathogenic bacteria with Escherichia coli WR1 as refer...
Article
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The wide host range phytopathogen D. dianthicola, first described in ornamentals in the1950s, rapidly became a threat for potato production in Europe and, more recently, worldwide. Previous genomic analyses, mainly of strains isolated from potato, revealed little sequence diversity. To further analyse D. dianthicola genomic diversity, we used a lar...
Article
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Glasshouse experiments were conducted to study infection and disease development in rockwool‐grown rose plants inoculated with Ralstonia pseudosolanacearum. A R. pseudosolanacearum strain isolated from rose plants was more aggressive than strains from anthurium or curcuma. The three rose cultivars tested, Avalanche, Red Naomi and Armando, differed...
Article
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In total 58 Xanthomona s strains isolated from Araceae worldwide, together with 13 other phylogenetically-related Xanthomonas strains, were characterized using multilocus sequence analysis based on concatenated sequences of seven single copy orthologous genes, extracted from whole genome sequences. The analysis revealed a monophyletic clade of 48 s...
Article
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Ralstonia pseudosolanacearum (Rps), previously known as R. solanacearum phylotypes I and III is one of the causal agents of bacterial wilt, a devastating disease that afects more than 250 plant species. Emerging Rps strains were identifed infecting new hosts. P824 Rps strain was isolated from blueberry in Florida. Rps strains including PD7123 were...
Article
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Peat use in horticulture is associated with a large ecological footprint. Peat is the predominant growing media in Europe. Modern cropping systems rely heavily on dynamic interactions of the crop with the microorganisms in the growing media and yet, in the search for sustainable peat-alternatives, the microbiome of the growing media has often been...
Article
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Experiments were carried out in 2012 and 2013 to answer two basic questions in the testing of potato blackleg causing agents before and after harvest. Firstly, what is the spatial distribution of symptomatic plants in the feld. Secondly, what is the distribution of infected tubers over the crates and the resulting detection probability using the st...
Article
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Plants have evolved complex defence mechanisms to avoid invasion of potential pathogens. Despite this, adapted pathogens deploy effector proteins to manipulate host susceptibility (S) genes, rendering plant defences ineffective. The identification and mutation of plant S genes exploited by bacterial pathogens are important for the generation of cro...
Article
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Managed aquifer recharge (MAR) can provide irrigation water and overcome water scarcity in agriculture. Removal of potentially present plant pathogens during MAR is essential to prevent crop diseases. We studied the die-off of three plant pathogenic bacteria in water microcosms with natural or filtered tile drainage water (TDW) at 10 and 25°C and w...
Article
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Background Bacterial plant pathogens of the Pectobacterium genus are responsible for a wide spectrum of diseases in plants, including important crops such as potato, tomato, lettuce, and banana. Investigation of the genetic diversity underlying virulence and host specificity can be performed at genome level by using a comprehensive comparative appr...
Article
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Shifts in the soil microbiome during continuous monoculture cropping coincide with increased suppressiveness against soil-borne diseases, as in the take-all decline of wheat. Here we report a similar phenomenon for bacterial blotch of mushrooms, caused by Pseudomonas ‘gingeri’, where ginger blotch incidence decreases during consecutive cycles of mu...
Chapter
Bacterial soft rot diseases devastate a wide range of crops, vegetables and ornamental plants worldwide. Amongst the most damaging agents of these diseases are members of the Pectobacterium and Dickeya genera belonging to the family Pectobacteriaceae in the order Enterobacterales. As an introduction to the topics of this book, this chapter presents...
Chapter
This chapter outlines isolation, detection and characterization methods for soft rot Pectobacteriaceae (SRP) and finishes with recommendations for diagnostics of SRP and perspectives for improved detection using metagenomic and pan-genomic approaches. For dilution plating and isolation of SRP, crystal violet pectate is still the medium of preferenc...
Chapter
The soft rot Pectobacteriaceae (SRP) cause diseases of significant economic impact on a wide range of crop and ornamental plants. By their nature these pathogens have a broad host range, a wide climatic tolerance and can be found in a variety of environmental niches (with knowledge of the latter likely to expand greatly in the coming years). While...
Chapter
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Management of soft rot Pectobacteriaceae (SRP) is a challenge as there are no control agents available and no effective resistance present in commercial cultivars. In addition, many species of SRP have a broad host range and spread via rotten plant material takes place readily. In this chapter, the possibilities for disease management are outlined....
Chapter
The soft rot Pectobacteriaceae (SRP) infect a wide range of plants worldwide and cause economic damage to crops and ornamentals but can also colonize other plants as part of their natural life cycle. They are found in a variety of environmental niches, including water, soil and insects, where they may spread to susceptible plants and cause disease....
Chapter
Soft rot Pectobacteriaceae (SRP) are ubiquitous on earth as there are records of findings from all continents where host plants are grown. This chapter describes information on soft rot diseases on these continents. For some countries, detailed information is provided by local experts on the SRP present, their economic damage, and the management st...
Article
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Identification and classification of members of the Ralstonia solanacearum species complex (RSSC) is challenging due to the heterogeneity of this complex. Whole genome sequence data of 225 strains were used to classify strains based on average nucleotide identity (ANI) and multilocus sequence analysis (MLSA). Based on the ANI score (>95%), 191 out...
Book
This book provides a detailed review of many different aspects of pathogens, from the effects of single base pair mutations to large-scale control options, bringing into a single volume over 100 years of findings from thousands of researchers worldwide. Diseases caused by soft rot Pectobacteriaceae (SRP) are a major cause of loss to crop, vegetable...
Article
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Xanthomonas fragariae is a quarantine bacterial pathogen that causes angular leaf spot on strawberry. The aim of our study was to analyse the mechanism of interaction of this bacterium with its host plant at the transcriptome level. For this purpose, mRNAs of X. fragariae growing in Wilbrink’s medium and from infected strawberry cv. Elsanta plants...
Article
Introduction: Bacterial blotch is one of the most economically important diseases of button mushrooms. Knowledge on mechanisms of disease expression, inoculum thresholds and disease management is limited to the most-well known pathogen, Pseudomonas tolaasii. Recent outbreaks in Western Europe have been attributed to 'P. gingeri' and P. salomonii f...
Article
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Background: Bacterial blotch is a group of economically important diseases affecting the cultivation of common button mushroom, Agaricus bisporus. Despite being studied for more than a century, the identity and nomenclature of blotch-causing Pseudomonas species is still unclear. This study aims to molecularly characterize the phylogenetic and phen...
Article
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Bacterial wilt and brown rot disease caused by Ralstonia solanacearum species complex (RSSC) is one of the major constraints of potato (Solanum tuberosum) production around the globe. During 2017-2018 an extensive field survey was conducted in six potato-growing provinces of Iran to monitor the status of bacterial wilt disease. Pathogenicity and ho...
Article
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Bacterial blotch is a group of economically important diseases of the common button mushroom (Agaricus bisporus). Once the pathogens are introduced to a farm, mesophilic growing conditions (that are optimum for mushroom production) result in severe and widespread secondary infections. Efficient, timely and quantitative detection of the pathogens is...
Article
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Ralstonia solanacearum the causal agent of bacterial wilt and brown rot disease is one of the major pathogens of solanaceous crops including potato around the globe. Biovar 2T (phylotype II/sequevar 25) of R. solanacearum is adapted to tropical lowlands and only reported from South America and Iran. So far, no genome resource of the biovar 2T of th...
Article
Potato soft rot Pectobacteriaceae (SRP) cause large yield losses and are persistent in seed lots once established. In Norway, different Pectobacterium species are the predominant cause of soft rot and blackleg disease. This work aimed to evaluate the potential of real-time PCR for quantification of SRP in seed tubers, as well as investigating the s...
Article
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Two TaqMan assays were developed for generic detection of Dickeya species in plant material. The assays enabled detection of all strains of D. chrysanthemi, D. dadantii, D. dianthicola, D. solani and D. zeae tested, but only weak reactions were found with strains of D. paradisiaca. The assays enabled detection of as low as 100 fg of target DNA in a...
Article
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Virus diseases are of high concern in the cultivation of seed potatoes. Once found in the field, virus diseased plants lead to declassification or even rejection of the seed lots resulting in a financial loss. Farmers put in a lot of effort to detect diseased plants and remove virus-diseased plants from the field. Nevertheless, dependent on the cul...
Article
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Glasshouse experiments were conducted to study the colonization of seedpods (siliques) and seeds of rapid cycling Brassica oleracea plants after spraying inoculum on clusters of recently opened flowers with Xanthomonas campestris pv. campestris (Xcc) at densities of 107–108 cfu ml−1. A green fluorescent protein (GFP) tagged Xcc strain was used to a...
Article
Potato blackleg is a tuber-borne bacterial disease caused by species within the genera Dickeya and Pectobacterium that can cause decay of plant tissue and wilting through the action of cell wall degrading enzymes released by the pathogen. In case of serious infections, tubers may rot before emergence. Management is largely based on the use of patho...
Article
Xylella fastidiosa is a heterogenous gram-negative bacterial plant pathogen with a wide host range covering over 300 plant species. Since 2013, in Europe, the presence of the pathogen is increasing in a part of the Mediterranean area, but it causes in particular severe disease problems in olive orchards in the Southern part of Italy. Various subspe...

Citations

... Families Helotiaceae, Piskurozymaceae, Exidiaceae or Myxotrichaceae were present in an important percentage at D00 and almost disappeared at D16. Other studies found abundance of genus Agaricus, Apiotrichum, Meliniomyces, Mycothermus, Candida and Pseudeurotium [28]. At species level (Supplementary material Fig. 12) we found the presence of Agaricus, Apiotrichum, Meliniomyces and Candida, among others, but not Meliniomyces or Mycothermus. ...
... Like other plant pathogens, Xam has a repertoire of effectors that can alter the structure or function of a host cell, create a more ideal environment for pathogen colonization, and overcome plant defense mechanisms (Boch [36], Hogenhout [37]). In the Xanthomonas and Ralstonia bacterial genera, this repertoire includes specialized transcription activator-like (TAL) effectors (Bodnar [38], Van Schie and Takken [39], Koseoglou [40]). TAL effectors are secreted into the plant cell and induce expression of plant susceptibility (S) genes that enhance disease. ...
... Porosity θ and longitudinal dispersivity α L [L] (α L = D /υ) were both determined from salt tracer experiments and used as input values to model bacterial BTC. Growth or die-off of the bacteria was not considered within the time span of the experiments (about 9 h) as we have shown in batch experiments that die-off in the water phase took only place after more than 24 h (Eisfeld et al., 2021). Hydrus-1D allows to compare the fit of a 1-or 2-site kinetic model by evaluating the coefficient of determination R 2 and the Akaike Information Criterion (AIC) as output data from Hydrus-1D. ...
... The high degree of openness of the Arthrobacter pangenome as compared to those of other genera (e.g., Janthinobacterium [83], Pectobacterium [84], Yersinia [85]), as well as the genes present in genomic islands and plasmids, suggests differences in adaptability among different taxa. As some microorganisms compete in communities strategically by leveraging catalysis kinetics or substrate affinities, it has been suggested that others may avoid direct competition via substrate specialization [26]. ...
... Portions of the cropping process were investigated regarding microbial communities, and their ecological niche, e.g. Vieira and Pecchia [10] and Cao et al. [15], investigated the bacterial community dynamics during the two-phase composting process, McGee et al. [16,17] investigated the microbial dynamics in compost during a commercial cropping process, Taparia et al. [18] evaluated the microbial community dynamics in different casing materials during an experimental cropping process, Martins et al. [19] investigated the bacterial community associated with blotch disease using asymptomatic and symptomatic mushroom caps collect from an organic farm and Carrasco et al. [20] examined the microbial community dynamics in compost and casing in a commercial cropping setting. These previous studies will be discussed along with the current results when appropriate. ...
... Pba belongs to a group of devastating soft rot-causing phytopathogenic bacteria (Soft Rot Pectobacteriaceae, SRP). These bacteria produce multiple PCW-degrading enzymes (PCWDEs), predominantly pectin-degrading, causing tissue maceration [11]. The plant polysaccharide degradation products are used by Pba as a growth substrate as well as "building blocks" for the assemblage of the specific "multicellular" biofilm-like structures-bacterial emboli [10,12]. ...
... D. dianthicola is one of the broad host range species within the group of soft rot Pectobacteriaceae (SRP) [3]. It was first described in a carnation (Dianthus) outbreak in the UK, the Netherlands and Denmark [4], and thereafter in several other European countries [5][6][7][8]. ...
... The worldwide spread of plant pathogens is mainly due to the trade of plants for planting as well as jumps of pathogens between plant species. This phenomenon has been observed for decades for species of the genera Pectobacterium and Dickeya [1]. These genera, belonging to the Pectobacteriaceae, cause soft rot, wilts, stunting and cankers on several crops, vegetables, ornamental plants and trees [1]). ...
... There are no potato cultivars available which are resistant to SRP, and no control agents are registered for treatment of plant material. Disease management in the European Union is predominantly based on the obligatory use of seed potatoes in which the disease is absent or only present in a low incidence (Van der Wolf et al. 2021b). Currently, seed testing and certification is solely based on field inspections. ...
... However, an increase in available whole-genome sequence data has improved species-level identification based on pairwise average nucleotide identity (ANI), in silico DNA-DNA hybridization (isDDH), and core genome multilocus sequence analysis (MLSA) (Zhang et al., 2016). There is little known about host-specific traits, as these species generally have broad host ranges (Van Gijsegem et al., 2021). In addition, there are no known resistance genes for potato soft rot, and it is therefore impossible to predict cultivar resistance without testing (Lyon, 1989;Czajkowski et al., 2011;Chung et al., 2013). ...