J.J. Pasantes's research while affiliated with University of Vigo and other places

Publications (85)

Poster
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DNA methylation is the best studied epigenetic marker, being essential in regulating gene expression and gene silencing in the eukaryotic genome. Different cell types have specific and pre-established DNA methylation profiles according to their organic function. Alterations in this profile may lead to cell malfunction, illness and, ultimately, tumo...
Article
The chromosomes of 346 specimens of Mytilus galloprovincialis, collected at four NW Iberian peninsula sites, were studied using a C-banding technique. Polymorphism for C-banding was detected. According to the banding patterns, three classes of mussels could be distinguished: 1) individuals showing two pairs of submeta/subtelocentric chromosomes bea...
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A de novo aberrant karyotype with 47 chromosomes including 2 different-sized markers was identified during prenatal diagnosis. Fluorescence in situ hybridization (FISH) with a Y painting probe tagged both marker chromosomes which were supposed to be isochromosomes of the short and the long arm, respectively. A normal boy was born in time who shows...
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Pericentric inversions of the human Y chromosome (inv(Y)) are the result of breakpoints in Yp and Yq. Whether these breakpoints occur recurrently on specific hotspots or appear at different locations along the repeat structure of the human Y chromosome is an open question. Employing FISH for a better definition and refinement of the inversion break...
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We characterized the DTF2 satellite DNA family of the clam Donaxtrunculus and compared its chromosomal localization with cytogenetic data revealed by fluorochrome banding, C-banding, and 28S rDNA FISH. In contrast to the other satellites detected previously in this species, DTF2 is an abundant (2%) GC-rich satellite that exhibits CpG methylation. S...
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ABSTRACT: Correction to Kirsch S, Pasantes J, Wolf A, Bogdanova N, Munch C, Pennekamp P, Krawczak M, Dworniczak B, Schempp W: Chromosomal evolution of the PKD1 gene family in primates. BMC Evolutionary Biology 2008, 8:263 (doi:10.1186/1471-2148-8-263).
Data
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Pairwise sequence identities among all gene/pseudogene intron 30 sequences. Intron 30 sequences of all gene/pseudogene copies are aligned by CLUSTALW, gap-containing positions removed and pairwise nucleotide sequence identities directly calculated from the CLUSTALW alignments.
Data
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GENECONV analysis of human and chimpanzee PKD1 intron 30 sequences. The first column lists the two sequences in which a significant fragment in the alignment was identified. Both p-values are multiple-comparison corrected for all sequence pairs, as well as for the length of the alignment. The SIM p-value indicates the likelihood to which such a sim...
Data
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Phylogenetic tree of PKD1 intron 30 sequences. Phylogenetic tree using sequences orthologous and paralogous to human intron 30 from human, chimpanzee, orangutan, and rhesus macaque. Human branch termini are labeled either by the functional gene or its pseudogene copies according to the nomenclature of NCBI Build 36. Chimpanzee branch termini are ei...
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The autosomal dominant polycystic kidney disease (ADPKD) is mostly caused by mutations in the PKD1 (polycystic kidney disease 1) gene located in 16p13.3. Moreover, there are six pseudogenes of PKD1 that are located proximal to the master gene in 16p13.1. In contrast, no pseudogene could be detected in the mouse genome, only a single copy gene on ch...
Article
There are only a few reports on the chromosomal location of DNA sequences in bivalve species, none of them using meiotic chromosomes. Mitotic chromosomes of the clam Dosinia exoleta were analysed by means of Giemsa, silver and fluorochrome staining and fluorescent in situ hybridization (FISH) with 18S + 28S rDNA and telomeric probes. A technique fo...
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Applying fluorescence in situ hybridisation (FISH), six cosmid clones of rhesus macaque origin containing the genes SACM2L, RING1, BAT1 and MIC2, MIC3, MICD, and MOG of the major histocompatibility complex (MHC) were localised to the long arm of the rhesus macaque chromosome 6 in 6q24, the orthologous region to human 6p21.3. Furthermore, centromere...
Article
Conflicting data has recently appeared concerning altered methylation patterns in interspecific mammalian hybrids and the potential this may hold for driving karyotypic evolution. We report no detectable methylation difference in the genomic DNA of different interspecific F1 antelope hybrids (family Bovidae) and their parent species using the methy...
Article
Molecular cytogenetic investigation of a male proband showing oligozoospermia (OAT I-II degrees ) has led to the detection of a Y-chromosome mosaicism. This mosaicism consists of a deleted Y chromosome with deletion of most of the long-arm heterochromatin, including the PAR2, del(Y), and a Y chromosome, which, in addition to that deletion, shows a...
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The chromosomes of Brachidontes rodriguezi were analysed by means of direct Giemsa staining, silver staining, fluorescent in-situ hybridization (FISH) with 18S + 28S rDNA probes, replication banding and chromomycin A3 (CMA) and DAPI fluorescence banding techniques. The diploid chromosome number in this species is 32 and the karyotype is composed of...
Article
During the last years it became obvious that a lot of families of long-range repetitive DNA elements are located within the genomes of mammals. The principles underlying the evolution of such families, therefore, may have a greater impact than anticipated on the evolution of the mammalian genome as a whole. One of these families, called chAB4, is r...
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Normal human Y and inverted Y chromosomes were chosen for physical fluorescence in situ hybridization (FISH) mapping of RBM and DAZ probes for the relative positioning of the RBM and DAZ gene clusters in interval 6 of the human Y chromosome. The inversion breakpoint in Yq11.23 turned out to be distal to the DAZ gene cluster, as the entire DAZ signa...
Article
The chromosomes of the mussel Mytilus galloprovincialis were analysed by means of chromomycin A3 (CMA), distamycin A/DAPI (DA/DAPI), DAPI/actinomycin D (DAPI/AMD) and chromomycin A3/distamycin A/DAPI (CDD) fluorescence banding techniques, C-banding, silver staining, N-banding and in situ hybridization with 18S+28S rDNA and telomere probes. 18S+28S...
Article
Several genes located within or proximal to the human PAR in Xp22 have homologues on the Y chromosome and escape, or partly escape, inactivation. To study the evolution of Xp22 genes and their Y homologues, we applied multicolour fluorescence in situ hybridization (FISH) to comparatively map DNA probes for the genes ANT3, XG, ARSD, ARSE (CDPX), PRK...
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The sister chromatid exchange (SCE) test is a widely used method to detect chemically induced genetic damage. This method has been applied in some aquatic species to monitor genetic pollutants in estuarine and marine environments. Dose response to 5-bromodeoxyuridine (BrdU) was investigated in the mussel Mytilus galloprovincialis Lmk. Experimental...
Article
A technique of sister chromatid differentiation (SCD) using bromodeoxyuridine (BrdU) incorporation and a modification of the fluorescence plus Giemsa (FPG) method was employed to determine cell-proliferation kinetics in gill tissue of the mussel Mytilus galloprovincialis. Dose-dependent proliferation inhibition was examined. In vivo administration...
Article
The silver staining patterns of the nucleolus organizer regions (NORs), an indication of rDNA transcriptional activity, were studied in metaphase chromosomes from larvae and juveniles of a bivalve mussel, Mytilus galloprovincialis. Two submetacentric/subtelocentric pairs of chromosomes, out of the the total complement of 14 pairs, were characterize...
Article
Human metaphase chromosomes 1 and 2 were examined by electron microscopy. Volumetric determinations were calculated on non-treated isolated chromosomes and spread metaphases treated with ammoniacal silver carbonate (ASC). Chromatid volume increased from early-metaphase to late-metaphase and this increase was statistically significant. A similar rel...
Article
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Mytilus galloprovincialis were collected from an intertidal population in NW Spain in 1988, and chromosomes from the gill tissue of 37 individuals were studied. The present paper describes the banding pattern of aM. galloprovincialis population which enabled us to identify all pairs of chromosomes. Banding was induced by means of a 2 SSC (0.3M sodi...