J. Liñán's scientific contributions

... Six different culture media were used to establish in vitro cultures of shoot tips: MS-0, Murashige and Skoog (1962) basal medium containing no growth regulators; MS-1, the same basal medium supplemented with 2 mg/l 6-benzyladenine (BA); C 2 D as described by Chée et al. (1984), also enriched with 2 mg/l BA; MS*/2, as used by Spiegel-Roy et al. (1985) containing 1.76 mg/l of 3-indolacetic acid (IAA) and 0.346 mg/l of gibberelic acid (GA 3 ), AND*, as proposed by Troncoso et al. (1988) with 0.1 mg/l naphtalenacetic acid (NAA) and 1 mg/l BA added; and, fi nally, C1*, also used by Troncoso et al. (1990), supplemented with 0.5 mg/l BA. To establish in vitro cultures of nodal segments MS-1 medium was used only. ...

... Due to differences between growth and development phases within cultivars, the germination responses of embryos could be different (Linan et al., 1999). However, in this experiment cultivars showed similar response of seed germination percent and seedling formation percent in each harvesting time, therefore it can be concluded that embryo growth phases of these three cultivars were approximately similar. ...

... Previous works testing single cultivars grafted on a range of potential interesting rootstocks indicate that the intrinsic vigor of some rootstock is transferred to the grafted cultivar. However, opposite results were also found in some cases, although it should be noted that explicit evaluation of vigor of the rootstock used was not carried out but based on previous literature (Troncoso et al., 1990;Romero et al., 2014). Similarly, different vigor control ability has been obtained for some rootstocks depending on the cultivar grafted, while a general dwarfing effect was observed for some others (Pannelli et al., 2002). ...

... Hence, In vitro propagation of endangered plants can offer considerable benefits for the rapid cultivation of species that are at risk, that have limited reproductive capacity and exist in threatened habitats (Fay, 1992). In vitro propagation methods are essential components of plant genetic resource management and they are becoming increasingly important for conservation of rare and endangered plant species (Sudha et al.,1998;Benson et al., 2000;Iankova et al., 2001;Bhatia et al., 2002).The Dendrobium fimbriatum is the second largest genus of Orchidaceae, which is composed of approximately 1500 species scattered in the world, and there is the most popular and highly valued orchids in the market (Chen and Ji, 1998). In vitro propagation of Dendrobium fimbriatum. ...

... Apart from the influence of AMF on nutrient uptake, other positive aspects of mycorrhization include raise of plant growth (Pasqualini et al., 2007;Sheng et al., 2009;Shokri and Maadi, 2009), increase of the rate of photosynthesis and transpiration, greater stomatal conductance (Caravaca et al., 2003;Wu and Zou, 2010), enhancement of nursery and post-trans planted response and decrease of transplanting stress (Carretero et al., 2009;, increase of plant tolerance to drought (Marulanda et al., 2007), salt stress (Sheng et al., 2009) and serpentine edaphic stress (Doubkova et al., 2012), as well as protection from nematodes attack , improvement of water use efficiency (Bolandnazar et al., 2007) and amelioration of fruit quality (Nzanza et al., 2012). ...

... Biotechnological tools like in vitro tissue culture provides an alternate way of screening of plants under in vitro conditions by exposing seeds, embryos or other explants to mediums provided with salts at different concentrations, without any interference with soil or climate [42]. In this study, preconditioned plumular apices of Halisbey cv. of peanut were exposed to different concentrations of NaCl and KCl under in vitro conditions with the objective to screen the explants that could tolerate with minimum loss in their activity and in vitro regeneration. ...

... Plants were obtained by micropropagation of axillary buds from individuals of the three study plants described above, according to López et al. (2004). The resulting plants were adapted to outdoor conditions following Cantos et al. (1993), transferred to individual plastic pots (diameter 11 cm) filled with perlite and placed in a glasshouse with minimum-maximum temperatures of 21-25°C, relative humidity of 40-60 % and natural daylight (minimum and maximum light flux: 200 and 1000 μmol m −2 s −1 , respectively). Pots were carefully irrigated with 20 % Hoagland's solution (Hoagland and Arnon 1938) as required. ...

... In particular a specific medium for recalcitrant olive varieties has been defined by undertaking an analysis of mineral elements in embryos or young shoots (Rugini, 1984). In spite of the extensive studies, the In vitro propagation of Olea europea species is still limited due to poor growth, slow lateral bud outgrowth and variable rooting ability of the explants (Rugini & Fedeli, 1990;Sarmiento et al., 2001;Saida et al., 2005). The problem is compounded by intraspecific variation in tissue culture responses between different cultivars. ...

... Wild relatives of the domesticated olive tree (Olea europaea L.) are evergreen, drought tolerant, usually multi-stemmed small trees or large shrubs with very good adaptability to different environmental conditions (Médail et al., 2001;Green, 2002;Kassa et al., 2019). Wild olives grow in arid and semiarid regions at different altitudes and soil types, including those exposed to severe water deficit, salinity and low temperatures (Cantos et al., 2002;Baldoni et al., 2006;Klepo et al., 2013;Belaj et al., 2016;Chiappetta et al., 2017). This adaptability to adverse environmental conditions makes wild olive trees suitable to grow in marginal soils (e.g., at risk of desertification), to colonize deforested habitats or to rehabilitate devastated regions (Bekele, 2005;Kassa et al., 2019). ...

... Our results related to plant growth are in accordance with many publications on olive that showed that plant growth (i.e., shoot length, total leaf area, dry weight, root length, and rooting ability) is inhibited by moderate and high salinity (Marin et al. 1995;Chartzoulakis 2005, Kchaou et al. 2010, 2013. Nitrogen is the most important mineral element in fertilization program because plants usually need N in greater amounts than other mineral nutrients (Hagag, Shahin, and El-Migeed 2011) with beneficial effects on vegetative growth which presumably reduces the non-productive phase (Garcia et al. 1999;Centeno et al. 2018). Several studies have been conducted to determine the appropriate rate and source for nitrogen fertilization for young olive trees. ...