J. Denduangboripant’s research while affiliated with Chulalongkorn University and other places

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Publications (106)


Fig. 1. Inflorescences and fruits of the twelve Mucuna species collected in Thailand (a) M. pruriens var. pruriens (L.) DC.; (b) M. pruriens var. utilis (Wall. ex Wight) Baker ex Burck; (c) M. bracteata DC. ex Kurz; (d) M. gigantea (Willd.) DC.; (e) M. gracilipes Craib; (f) M. hainanensis Hayata; (g) M. interrupta Gagnep.; (h) M. macrocarpa Wall.; (i) M. monosperma DC. ex Wight; (j) M. revoluta Wilmot-Dear; (k) M. thailandica Niyomdham & Wilmot-Dear; (l) M. warburgii Lauterb. & K. Schum.
Fig. 2. HPLC fingerprint of the L-dopa reference standard and seven Mucuna seeds: (a) M. pruriens, (b) M. bracteata, (c) M. gigantea, (d) M. interrupta, (e) M. macrocarpa, (f) M. monosperma, and (g) M. revoluta. The scale bar in each figure indicates a length of 1 cm. The arrows indicate the Ldopa peak.
Fig. 3. Relative distribution of the K2P pairwise distance of DNA barcode regions, including ITS, ITS2, matK, rbcL and trnH-psbA, among twelve Mucuna species.
Fig. 4. Relationship of the ML and MP phylogenies of twelve Mucuna species and Pueraria candollei. (A) The maximum likelihood tree of the combined nucleotide sequence data matrix. The best ML tree was retrieved with a score likelihood of ln = 7067.519. Numbers along branches are the 1000-replicate bootstrap supporting values of each branch. (B) The most parsimonious tree of the combined nucleotide sequence data matrix. The MP tree was found by the branch-and-bound search method with a tree length of 618. Numbers above and under the branches are the evolutionary step changes and bootstrap supporting values of each branch, respectively.
Fig. 5. The aphrodisiac herbal products (PP1-10) and HPLC fingerprint. (A) Aphrodisiac herbal products collected from the e-commerce platform. (B) HPLC fingerprint of ten aphrodisiac herbal products compared with the L-dopa reference standard.

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HPLC and DNA barcoding profiles for identification of the selected twelve Mucuna species and its application for detecting prohibited aphrodisiac Mucuna products
  • Article
  • Full-text available

February 2023

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533 Reads

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4 Citations

Heliyon

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Jessada Denduangboripant

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[...]

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Suchada Sukrong

Aphrodisiac herbal products originated from various plants including Mucuna species. In Thai folklore, Mucuna macrocarpa Wall. and M. pruriens (L.) DC. have long been consumed and utilized for their aphrodisiac properties. Consumption of these plants can lead to serious adverse effects caused by l-dopa. The plants have been legally banned for use as foods, dietary supplements, or nutraceuticals by the FDA of several countries. To protect consumers, methods for the identification of illicit plants or herbal products are needed. This study aimed to identify the selected twelve Mucuna species and examine the aphrodisiac herbal products containing M. macrocarpa and M. pruriens by using HPLC analysis of l-dopa coupled with DNA barcoding profiles of ITS, matK, rbcL, and trnH-psbA. The results showed that l-dopa could be found not only in the seeds of M. macrocarpa and M. pruriens but also in associated allied Mucuna species. Then, a DNA barcode was introduced to support in HPLC profiling to identify the plants. DNA barcodes of twelve Mucuna species found in Thailand were established and used to reconstruct a phylogenetic tree. In this study, ITS2 sequences showed the highest interspecific variability and could be used to differentiate all Mucuna species. The results of ITS2 sequence coupled with HPLC analysis revealed that all the purchased aphrodisiac products originated from M. pruriens only. Therefore, the integration of HPLC analysis and DNA barcoding profile was an efficient method for the identification of prohibited Mucuna species for safety monitoring of herbal supplements and protecting customer safety. Regulatory agencies should raise awareness and restrain the use of these commercial products.

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Phylogeny of Globba section Nudae and taxonomic revision of the new Globba subsection Pelecantherae

February 2022

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780 Reads

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5 Citations

Plant Systematics and Evolution

A molecular systematic study of Globba section Nudae (Zingiberaceae) using ITS and matK sequences identifies three major clades, Globba subsection Nudae, G. subsection Mediocalcaratae and a new subsection, Globba subsection Pelecantherae, which is described here. The two species belonging in this subsection, Globba pelecanthera and Globba securifer, which are both new, are described. Rectangular anther appendages are reported in Globba for the first time. Evidence of hybridisation is given. The morphological characters of the flowers, which are likely to be important in pollination, are discussed.


Fig. 1. Bar-HRM analysis of species of Bacopa. (A) Multiple sequence alignments of PCR amplicons obtained from species of Bacopa using the trnL-F MP primer pair. (B-D) TrnL-F barcoding coupled with HRM analysis to differentiate three species of Bacopa, B. monnieri, B. caroliniana, and B. floribunda, shown in terms of (B) melting peak, (C) normalized plot, and (D) difference plot.
Table 2 . Primers used in this study.
Table 3 . GenBank search results and sequence analysis of the six selected DNA barcodes from the plants of the genus Bacopa. DNA marker
DNA barcoding of Bacopa species coupled with high-resolution melting analysis

November 2018

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1,127 Reads

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7 Citations

In Thailand, there are three species of Bacopa, namely, B. monnieri, B. caroliniana, and B. floribunda. Among these species of Bacopa, B. monnieri is the only medicinal species, used for the treatment of cognitive impairment and improvement of cognitive abilities because of its bioactive constituents, bacoside A and B. However, because of the similar characteristics of these species, it is difficult to differentiate among related species, resulting in confusion during identification. For this reason, and to ensure therapeutic quality for consumers, authentication is important. In this study, the three abovementioned species of Bacopa were evaluated using barcoding coupled with high-resolution melting (Bar-HRM) analysis based on primers designed for the trnL-F sequences of the three species. The melting profiles of the trnL-F amplicons of B. caroliniana and B. floribunda were clearly different from the melting profile of the trnL-F amplicon from B. monnieri; thus, the species could be discriminated by Bar-HRM analysis. Bar-HRM was then used to authenticate commercial products in various forms. The melting curves of the six commercial samples indicated that all the tested products contained genuine B. monnieri species. This method provides an efficient and reliable authentication system for future commercial herbal products and offers a reference system for quality control.


DNA barcoding of Aristolochia plants and development of species-specific multiplex PCR to aid HPTLC in ascertainment of Aristolochia herbal materials

August 2018

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481 Reads

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29 Citations

The anecdotal evidence is outstanding on the uses of Aristolochia plants as traditional medicines and dietary supplements in many regions of the world. However, herbal materials derived from Aristolochia species have been identified as potent human carcinogens since the first case of severe renal disease after ingesting these herbal preparations. Any products containing Aristolochia species have thus been banned on many continents, including Europe, America and Asia. Therefore, the development of a method to identify these herbs is critically needed for customer safety. The present study evaluated DNA barcoding of the rbcL, matK, ITS2 and trnH-psbA regions among eleven Aristolochia species collected in Thailand. Polymorphic sites were observed in all four DNA loci. Among those eleven Aristolochia species, three species (A. pierrei, A. tagala and A. pothieri) are used as herbal materials in Thai folk medicine, namely, in Thai “Krai-Krue”. “Krai-Krue” herbs are interchangeably used as an admixture in Thai traditional remedies without specific knowledge of their identities. A species-specific multiplex PCR based on nucleotide polymorphisms in the ITS2 region was developed as an identification tool to differentiate these three Aristolochia species and to supplement the HPTLC pattern in clarifying the origins of herbal materials. The combination of multiplex PCR and HPTLC profiling achieves accurate herbal identification with the goal of protecting consumers from the health risks associated with product substitution and contamination.


S1 Fig

August 2018

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20 Reads

Sequence alignment of full length rbcL genes of eleven Aristolochia plants. The numbers on the top line represent the base numbers in sequence alignment. The altered bases indicate the sequence differences. ‘.’ represents the base being identical to the first sequence. The first and the last three nucleotides are start and stop codon, respectively. (PDF)


S2 Fig

August 2018

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20 Reads

Sequence alignment of full length matK genes of eleven Aristolochia plants. The numbers on the top line represent the base numbers in sequence alignment. The altered bases indicate the sequence differences. ‘.’ represents the base being identical to the first sequence. The first three nucleotides are start codon and the last three nucleotides are stop codon. (PDF)





DNA Barcoding of Aristolochia Plants and Development of Species-Specific Multiplex PCR to Aid HPTLC in Ascertainment of Aristolochia Herbal Materials v2

July 2018

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3 Reads

The anecdotal evidence is outstanding on the uses of Aristolochia plants as traditional medicines and dietary supplements in many regions of the world. However, herbal materials derived from Aristolochia species have been identified as potent human carcinogens since the first case of severe renal disease after ingesting these herbal preparations. Any products containing Aristolochia species have thus been banned on many continents, including Europe, America and Asia. Therefore, the development of a method to identify these herbs is critically needed for customer safety. The present study evaluated DNA barcoding of the rbcL, matK, ITS2 and trnH-psbA regions among eleven Aristolochia species collected in Thailand. Polymorphic sites were observed in all four DNA loci. Among those eleven Aristolochia species, three species (A. pierrei, A. tagala and A. pothieri) are used as herbal materials in Thai folk medicine, namely, in Thai “Krai-Krue”. “Krai-Krue” herbs are interchangeably used as an admixture in Thai traditional remedies without specific knowledge of their identities. A species-specific multiplex PCR based on nucleotide polymorphisms in the ITS2 region was developed as an identification tool to differentiate these three Aristolochia species and to supplement the HPTLC pattern in clarifying the origins of herbal materials. The combination of multiplex PCR and HPTLC profiling achieves accurate herbal identification with the goal of protecting consumers from the health risks associated with product substitution and contamination.


Citations (20)


... DNA barcoding profiles of ITS, matK, rbcL, and trnH-psbA are used to support HPLC profiling to identify M. pruriens (L.) DC plants. It is an efficient method for the identification of the herbal for safe commercial products (Intharuksa et al., 2023). ...

Reference:

COMPARISON IN DNA BARCODING, GENE EXPRESSION AND CHEMICAL OF PUERARIA MIRIFICA PLANTS
HPLC and DNA barcoding profiles for identification of the selected twelve Mucuna species and its application for detecting prohibited aphrodisiac Mucuna products

Heliyon

... Thailand boasts one of the richest diversities of Zingiberaceae plants, with the family being notably abundant and diverse in the country's flora. Thailand is home to approximately 29 accepted genera and over 400 species of Zingiberaceae (Larsen and Larsen, 2006;Saensouk et al., 2007;Maknoi et al., 2011;Saensouk and Saensouk, 2014;Saensouk et al., 2016;Škorničková et al., 2017;Saensouk et al., 2018;Boonma and Saensouk, 2019;Saensouk and Saensouk, 2019a, b;Sangvirotjanapat et al., 2019a, b;Boonma and Saensouk, 2020;Boonma et al., 2020a, b;Saensouk and Saensouk, 2020a, b;Boonma et al., 2021;Saensouk and Saensouk, 2021a, b, c, d;Saensouk et al., 2021a, b, c, d, e;Sangvirotjanapat et al., 2021;Ye et al., 2021;Boonma et al., 2022;Ragsasilp et al., 2022;Rakarcha et al., 2022;Saensouk et al., 2022a, b, c, d, e, f;Boonma et al., 2023;POWO, 2023). The tropical climate and diverse ecosystems found in Thailand create favorable conditions for the growth and proliferation of Zingiberaceae species. ...

Phylogeny of Globba section Nudae and taxonomic revision of the new Globba subsection Pelecantherae

Plant Systematics and Evolution

... DNA regions were amplified and sequenced for the nuclear ribosomal RNA internal transcribed spacer (ITS) region using the primers p5F,p3F, p2R, and p4R (Baldwin 1992;Cheng et al. 2016), for the plastid ndhF gene using primers 972F, 1318F, 1318R, 1603F, 1603R, and 1955R (Olmstead and Sweere 1994, and for the plastid trnL intron and trnL-trnF intergenic spacer (collectively identified herein as the 'trnL-trnF region') using the primers c, d, e, and f (Taberlet et al. 1991), using methods described by Tippery et al. (2020). Newly obtained sequences were aligned manually to one another and to sequences that were retrieved from GenBank (Olmstead et al. 2001;Fritsch et al. 2007;Estes and Small 2008;Kuzmina et al. 2017;Scatigna et al. 2018Scatigna et al. , 2022Tungphatthong et al. 2018) in the program Mesquite ver. 3.81 (Maddison and Maddison 2023). ...

DNA barcoding of Bacopa species coupled with high-resolution melting analysis

... Thus, other methods for differentiating these plants are needed, such as chemical profiling, molecular cytogenetics, and molecular marker analysis. However, chemical patterns also have limitations, as the chemical contents can be influenced by factors such as cultivation, weather conditions, and harvesting time [20]. A few molecular cytogenetics studies have reported that C. comosa cultivars have chromosome numbers 2n = 63 and 42 [21,22], whereas C. latifolia has chromosome numbers 2n = 63 and 84 [9]. ...

DNA barcoding of Aristolochia plants and development of species-specific multiplex PCR to aid HPTLC in ascertainment of Aristolochia herbal materials

... Compared to RFLP, these PCR based markers are preferred because of the relative ease with which PCR assays can be carried out. Both RAPD (Xu et al. 1998;Del Piano et al. 2000;Evanno et al. 2005;Zhang et al. 2005Zhang et al. , 2008Arslan and Okumus 2006;Sarala and Rao 2008;Sivaraju et al. 2008;Denduangboripant et al. 2010;D'hoop et al. 2010) and AFLP (Huang et al. 2008;Zhang et al. 2008;Chuanyin et al. 2009;Liu et al. 2009) were used to analyze the genetic diversity and varietal identification in tobacco. ...

Genetic Polymorphism between Tobacco Cultivar-groups Revealed by Amplified Fragment Length Polymorphism Analysis

... Disparities in scyllarid body forms have often been attributed to environmental factors and behavioral adaptations for habitat exploitation (Jones, 2007;Spanier et al., 2010). It would be informative to see how the species differ now, since studies indicate different preferences in water depth and sediment composition for each species (Jones, 1993(Jones, , 2007Iamsuwansuk et al., 2012). Phenotypic variation between sites is analyzed to identify discrete populations within the region. ...

Molecular and Morphological Investigations of Shovel-Nosed Lobsters Thenus spp. (Crustacea: Decapoda: Scyllaridae) in Thailand

Zoological Studies

... To date, numerous studies have identified and analyzed tobacco cultivars at the molecular level using markers such as AFLP (Ren andTimko 2001, Sarcevic et al. 2013), RAPD (Zhang et al. 2005, Sun et al. 2020, SSR (Moon et al. 2008, Gholizadeh et al. 2012, Zhang et al. 2013, ISSR (Li et al. 2008, Denduangboripant et al. 2012, Qi et al. 2012, DArT (Maryan et al. 2012, Lu et al. 2013, and SNPs (Zhang et al. 2017), or combinations thereof, to perform genetic diversity assessments and fingerprint mapping. Additionally, limited study has reported the rapid identification of tobacco cultivars using cured tobacco leaves with SSR, SCAR, and RAPD markers (Sun et al. 2020). ...

Determination of Local Tobacco Cultivars Using ISSR Molecular Marker
  • Citing Article
  • May 2010

Chiang Mai Journal of Science

... The first phylogenetic study of Goniothalamus (Yuyen et al., 2007) was based on sequences of two chloroplast DNA markers (trnL and trnG introns), with taxon sampling limited to only 20 accessions (13 described species) from Thailand. One of the aims of the study was to assess the congruence of the phylogeny with Boerlage's (1899) and Bân's (1974) infrageneric classifications, although this was not convincingly addressed as most clades lacked adequate support. ...

A study of Goniothalamus (Annonaceae) in Thailand based on chloroplast trnL and trnG intron sequences

... Long-term high concentrations of heavy metal damage microorganisms in the soil to different degrees and affect the distribution of the microbial population [23]. Additionally, to cope with stress caused by heavy metals, microorganisms have evolved mechanisms to overcome toxicity, including metal reduction, cell permeability reduction, and extracellular isolation [24]. Therefore, heavy metal-immobilizing bacteria screened from in situ heavy metal-contaminated plant rhizosphere soil show better environmental adaptability and higher ability for preventing crops from absorbing heavy metals [25]. ...

Erratum to “Heavy metal tolerant Metalliresistens boonkerdii gen. nov., sp. nov., a new genus in the family Bradyrhizobiaceae isolated from soil in Thailand” [Syst. Appl. Microbiol. 33 (2010) 374–382]. Proposal of Rhodopseudomonas boonkerdii sp. nov., a new heavy metal tolerant bacterium isolated from Thailand
  • Citing Article
  • April 2011

Systematic and Applied Microbiology

... The minor nucleolus/NOR chromosome configurations found for the plants here will likely represent artifacts of the squash preparations of the PMCs (Fig. 5). The findings confirm previous studies for S. rexii using fluorescent in situ hybridization with 45S-nrDNA probes, where one large satellite signal and one small nrDNA signal were found (Denduangboripant et al. 2007, Möller et al. 2008. This strongly suggests that the chromosomes associated with the nucleoli were NOR chromosomes and that S. grandis has one terminal NOR locus per genome. ...

Variation and Inheritance of Nuclear Ribosomal DNA Clusters in Streptocarpus (Gesneriaceae) and Their Biological and Phylogenetic Implications

International Journal of Plant Sciences