J. B. Free’s research while affiliated with Central Tuber Crops Research Institute and other places

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Publications (124)


The behavior of queen honeybees and their attendants
  • Article

March 1992

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51 Reads

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34 Citations

Physiological Entomology

J. B. FREE

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J. R. SIMPKINS

The behaviour of queen and worker honeybees (Apis mellifera L.) was observed using small colonies in observation hives. Workers paid more attention to queens which had been mated for 2 months or more than to those which were newly mated; virgin queens received least attention. Queens received most attention when they were stationary and least when they were walking over the comb; virgin queens were most active. Queen cells had as many attendants as virgin queens and queen larvae were inspected almost continuously. The queen pheromone component 9–oxo‐trans‐2–decenoic acid stimulated ‘court’ behaviour when presented on small polyethylene blocks, but workers responded aggressively to complete extracts of queens' heads. Both the heads and abdomens of mated queens received much attention from court workers but the abdomens were palpated by more workers for longer and were licked much more. The queens' thoraces were least attended. Abdominal tergites posterior to tergite glands were licked for longer than those anterior to the glands. Only worker bees very near to the queen reacted to her and joined her ‘court’. No evidence was found of a diel periodicity in the behaviour of a queen or her ‘court’. During the winter the queen's court was smaller than in summer and she walked less and laid fewer eggs. When colonies were fed with sucrose syrup in winter, their queens laid more eggs and workers reared more brood but there was no change in the attention received by the queens. The implications of these findings for the secretion and distribution of queen pheromones are discussed.


Queen discrimination by honeybee (Apis mellifera L) workers

January 1990

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8 Reads

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5 Citations

Apidologie

Two queens were caged separately with groups of young sibling workers which were the daughters of another queen. The cages were exposed to the same environmental odours for 10 days. When placed in the test apparatus and given a choice of both queens, workers segregated towards the queen with which they had been caged. This provides further evidence that the distinctive odour of an individual queen is probably partly inherited and is learned by workers. -from Authors


The effect of different periods of brood isolation on subsequent brood-cell visits by worker honeybees (Apis mellifera l.)

January 1989

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5 Reads

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11 Citations

Cells containing honeybee larvae were isolated from contact with workers for periods from 15 min to 24 h. Those which had been isolated for up to 90 min, subsequently received more worker visits than did unisolated ones. Isolation for periods of 3 h or more led to increases in larval mortality. In one test, isolated cells which contained eggs were also visited more than unisolated controls.


Honeybee responses to chemical components from the worker sting apparatus and mandibular glands in field tests

January 1989

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3 Reads

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32 Citations

Twenty-four chemical components from the sung apparatus and 2-heptanone from the mandibular glands of the worker honeybee (Apis mellifera L.) were tested at the hive entrance for their effect on alerting, slinging, Nasonov gland exposure, and flight activity. They were further tested at experimental food sources for their effect on foraging activity. In each bioassay, 33–66% of the components appeared to have some effect. Only 2 components, iso-pentyl acetate and l-pentanol, elicited a response in all tests, and 4 of the less-volatile components elicited no response in any test in which they were presented. Some components elicited different responses when they were presented at different concentrations. Some components had an inhibitory effect on flight and foraging from the hive entrance, whereas others had a stimulatory effect. When presented at food sources, many components were repellent to foragers; a mixture of 2 components, n-ocryl acetate and benzyl acetate, had the maximum repellent effect. The roles of these pheromone components in the control of colony activities, and the opportunities for improving honeybee colony management by the use of synthetic pheromones, are discussed.



Using synthetic pheromone lures to attract honeybee colonies in Kenya

January 1986

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49 Reads

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8 Citations

Nasonov pheromone lures, either with or without (E)-9-oxo-2-decenoic acid (902), were hung inside empty Kenya top-bar hives in each of 4 apiaries in Kenya. Of the 48 hives with lures, 24 were occupied by honeybee swarms, although at one site all hives, with or without lures, remained unoccupied. At the other 3 sites, 56, 78 and 17% of hives with Nasonov lures, with Nasonov + 902 lures, and without lures, respectively, were occupied. The difference between hives with Nasonov lures and with Nasonov + 902 lures was not significant. It is concluded that lures should be placed in hives only at those times of year when migrating colonies usually settle in the area concerned.


Repelling foraging honeybees with alarm pheromones
  • Article
  • Full-text available

October 1985

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420 Reads

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33 Citations

The Journal of Agricultural Science

J. B. Free

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[...]

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M. C. Smith

The honeybee alarm pheromones 2·heptanone and isopentyl acetate were repellent to honeybee foragers when applied to plots of oil-seed rape and field beans and to sunflower heads. Their possible use to repel bees from crops before insecticide application is discussed.

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Influence of virgin queen honeybees (Apis mellifera) on queen rearing and foraging

March 1985

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43 Reads

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13 Citations

Physiological Entomology

Virgin queens are as effective as mated laying queens at inhibiting colonies from rearing queens but not from producing queen cell cups. Colonies without brood produce fewer queen cell cups than similar colonies that have brood. Colonies without queens forage much less and collect less pollen than with either a mated or virgin queen. Colonies with virgin queens forage as much as those with mated queens but collected less pollen.


Influence of immature queen honeybees (Apis mellifera L.) on queen rearing and foraging

March 1984

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19 Reads

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11 Citations

Physiological Entomology

Providing queenless colonies with five queen cells containing larvae or pupae diminished the number of queen cells and queen cell cups subsequently produced, but not as effectively as the provision of a mated laying queen. Immature queens were more effective than the mature queens in stimulating pollen collection, but were less effective in stimulating nectar collection.



Citations (90)


... What is needed is a BSF fly cage that functions as a place for BSF to mate and produce eggs until hatching (Čičková et al., 2015). The cage is covered with wire or gauze and put in a place exposed to sunlight (Free & Williams, 1979). For the place of laying eggs for female BSF flies, it is necessary to prepare cardboard, wood, or boards that have gaps (Chia et al., 2018). ...

Reference:

COMMUNITY SERVICE VILLAGE DEVELOPMENT PARTNER PKM- PDM WASTE INDEPENDENT VILLAGE IN KELING JEPARA VILLAGE
Communication by Pheromones and other Means in Apis Florea Colonies
  • Citing Article
  • January 1979

... Natural Nasonov pheromone is a mixture of six monoterpenes: (E) citral, (Z) citral, geraniol, nerol, geranic acid, nerolic acid, plus the sesquiterpene (E,E)-farnesol, with the approximate ratio of 1:1:1:100:75:12.5:50. The synthetic Nasonov pheromone used in this study consists of a 1:1:1 mixture of citral, geraniol, and nerol [24][25][26]30]. The most important components for clustering are reported to be (E) citral, geraniol, and nerolic acid [31], though other studies have suggested that all seven terpenoids are required to achieve a full attractive effect [32]. ...

Use of Unpurified Nasonov Pheromone Components to Attract Clustering Honeybees
  • Citing Article
  • January 1982

... Both modes of pollination (self or cross) have been reported in mangoes; however, the results of pollination studies vary greatly due to cultivar differences in modes of pollination (Dutta et al., 2013;Huda et al., 2015;Ramírez and Davenport, 2016). Several studies indicate great benefits of insect pollination (Dag et al., 2009;Ramírez and Davenport, 2016), whilst others indicate wind as an effective pollination vector for mango (Free and Williams, 1976;Sousa et al., 2010). However, insignificant pollen flow in air currents has also been observed (Singh, 1961). ...

Insect pollination of Anacardium occidentale L., Mangifera indica L., Blighia sapida Koenig and Persea americana Mill
  • Citing Article
  • January 1976

Tropical Agriculture

... These distinct plant morphs are planted in rows or bays of rows, and pollinators travelling directly from male flowers deposit much higher canola pollen loads (32.19 grains) on female stigmas than pollinators travelling from female to female flowers (1.73 grains) (Waytes 2017). However, honey bees have been known to orient themselves to the rows and forage up and down rather than crossing between rows or bays (Free 1966, Thorp 1979, and the relative contribution of nectar and pollen foragers to hybrid seed pollination is unclear. ...

THE FORAGING BEHAVIOUR OF BEES AND ITS EFFECT ON THE ISOLATION AND SPECIATION OF PLANTS
  • Citing Chapter
  • December 1966

... However, when crosspollination takes place, fruit quality -in terms of fruit size and symmetry-significantly improves (Sedgley and Griffin 1989;Bellini 1993;. In Belgium, the variety Doyenne do Comice is commonly planted in the same orchard as pollinizer given its compatibility and bloom synchrony (Free and Spencer-Booth 1964). In practice, plant growth hormones such as gibberellins are also commonly used to improve fruit set artificially, resulting in fruits that are entirely or partially seedless (Richard et al. 2001;Tromp and Wertheim 2005). ...

The Effect of Distance From Pollinizer Varieties on the Fruit Set of Apple, Pear and Sweet-Cherry Trees
  • Citing Article
  • January 1964

Journal of Horticultural Science

... vulgaris L.), P. coccineus requires cross-pollination, which is performed mainly by bees, bumblebees and hummingbirds, the majority of which are wild (Burquez and Sarukhán, 1980;Labuda, 2010). Studies of pollination in this crop have focused on the characterization of floral visitors (Burquez and Sarukhán, 1980) and the mechanisms of pollination (self-pollination and cross-pollination) in the first days of flowering (Williams and Free, 1975), as well as the efficiency of different pollinator species in terms of reproductive success (Free, 1966;Kendall and Smith, 1976;Pando et al., 2011;Tchuenguem et al., 2014). However, no studies have been directed towards determining whether the community of floral visitors of P. coccineus and its activity vary as a function of the type of agricultural management system and flower color. ...

The Pollination and Set of the Early Flowers of Runner Bean (Phaseolus Coccineus L.)
  • Citing Article
  • January 1975

Journal of Horticultural Science

... In addition to distance between cultivars, the arrangement of cultivars can influence pollen flow and cross-pollination rates (Fig. 3). For example, mixing cultivars within the same row may enhance cross-pollination by bees given that many bees including honey bees Apis mellifera frequently forage in a linear pattern known as traplining (Free and Spencer-Booth 1963;Jackson and Clarke 1991;Cresswell et al. 1995;Cresswell 1997;Lihoreau et al. 2013). Such linear foraging behaviors, combined with preferences and floral constancy to certain cultivars, have been shown to limit cross-pollination in crops like blueberries (Estravis- Barcala et al. 2021) and almonds (Jackson 1996). ...

The Foraging Areas of Honey-Bee Colonies in Fruit Orchards
  • Citing Article
  • January 1963

Journal of Horticultural Science

... Pseudogermination occurs when the pollen grain initiates a germination sequence, but is interrupted prior to full pollen tube formation. A significant body of work shows that pollen collected and stored by honey bees has reduced capacity to pollinate female flowers, in part due to loss of germination potential (Singh and Boynton 1949;Griggs and Vansell 1950;Griggs et al. 1953;Johansen 1956;Kraai 1962;Free and Durrant 1966;Mesquida and Renard 1989;Vaissiere et al. 1996). Post collection, further processing and storage in the comb may change germination potential as well. ...

The Transport of Pollen by Honey-Bees from one Foraging Trip to the Next
  • Citing Article
  • January 1966

Journal of Horticultural Science

... First, it was demonstrated that bees adapt to their own alarm pheromones. When a dispenser containing synthetic alarm substances is placed into a hive, within 1 h the bees become less inclined to sting and do not differentiate between scented and control targets (Al-Sa'ad et al., 1985;Free, 1988). Under natural conditions, however, the high volatility of IAA makes adaptation very unlikely. ...

Adapting honeybees (Apis mellifera l.) to synthetic alarm pheromones to reduce aggression
  • Citing Article
  • January 1988

... Globally, various communities have developed different attractant baits for bee swarms. To date, the most researched and effective attractants reported are the synthetic blends of queen mandibular pheromone (QMP) and Nasonov gland pheromone (NGP) from workers (Bortolotti & Costa, 2014;Kigatiira et al., 1986;Schmidt, 1999;Winston et al., 1993). The QMP is naturally produced in the mandibular glands of queen honey bees to regulate several colony behaviours, including swarming (Bortolotti & Costa, 2014;Grozinger et al., 2014). ...

Using synthetic pheromone lures to attract honeybee colonies in Kenya
  • Citing Article
  • January 1986