J Amara’s research while affiliated with University of Milan and other places

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Publications (3)


Fig. 1 Human basophil degranulation induced either by anti-IgE anti-serum (•) diluted tenfold from 1 x 10
Fig. 2 Electrophoresis (polyacrylamide 7-15%. bands revealed by silver staining): samples numbered 1 to 5 are standards for the blind experiments a. c. e, h, m, p. Lane 1. Molecular weight standards for electrophoresis; lane 2, monoclonal IgG added with human serum albumin; lane 3. Tyrode's buffer without human serum albumin; lane 4.1 x 10 2 anti-IgE dilution; lane 5.1 x 10 3 dilution. Samples tested Wind: a and c, buffer; e, 1 x 10 36 anti-IgE dilution: h. 1 x 10 2 anti-IgE dilution; m, 1 x 10 3 anti-IgE dilution; p. 1 x 10 35 anti-IgE dilution.
Human basophil degranulation triggered by very dilute antiserum against IgE
  • Article
  • Full-text available

June 1988

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420 Reads

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240 Citations

Nature

E Davenas

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J Amara

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[...]

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J Benveniste

When human polymorphonuclear basophils, a type of white blood cell with antibodies of the immunoglobulin E (IgE) type on its surface, are exposed to anti-IgE antibodies, they release histamine from their intracellular granules and change their staining properties. The latter can be demonstrated at dilutions of anti-IgE that range from 1 x 10(2) to 1 x 10(120); over that range, there are successive peaks of degranulation from 40 to 60% of the basophils, despite the calculated absence of any anti-IgE molecules at the highest dilutions. Since dilutions need to be accompanied by vigorous shaking for the effects to be observed, transmission of the biological information could be related to the molecular organization of water.

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Human basophil degranulation triggered by very dilute antiserum against IgE

June 1988

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4,510 Reads

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150 Citations

Nature

When human polymorphonuclear basophils, a type of white blood cell with antibodies of the immunoglobulin E (IgE) type on its surface, are exposed to anti-IgE antibodies, they release histamine from their intracellular granules and change their staining properties. The latter can be demonstrated at dilutions of anti-IgE that range from 1 x 10(2) to 1 x 10(120); over that range, there are successive peaks of degranulation from 40 to 60% of the basophils, despite the calculated absence of any anti-IgE molecules at the highest dilutions. Since dilutions need to be accompanied by vigorous shaking for the effects to be observed, transmission of the biological information could be related to the molecular organization of water


Fig. 1 Human basophil degranulation induced either by anti-IgE anti-serum (•) diluted tenfold from 1 x 10 2 down to 1 x l0 60 (a) or hundredfold down to 1 x 10 120 (b) or by anti-IgG antiserum (O) diluted hundredfold from 1 x 10 2 down to 1 x 10 120 (representatives of at least 10 experiments for anti-IgE and 4 experiments for anti-IgG). The significant (P < 0.05) percentage of degranulation was 15% (a) and 20% (b). (....) relation to the number of counted basophils from control wells'''. Methods Goat anti-human IgE (Fc) antiserum or as a control, goat anti-human IgG (Fc) antiserum (Nordic Immunology, The Netherlands) was serially diluted as indicated above in HEPESbuffered Tyrode's solution (in g 1-1 : NaCl, 8; KCl. 0.195;HEPES,2.6;EDTANa 4. 1.040; glucose, 1 human serum albumin (HSA), 1.0; heparin. 5000 U per 1; pH 7.4). Between each dilution, the solution was thoroughly mixed for 10 s using a Vortex. Given the molecular weight of IgG molecules (150,000). the 1 x 10 60 and 1 x 10 120 dilutions correspond in the assay to 2.2 x 10-66 M(th)and 2.2x l0-126 
Fig. 2 Electrophoresis (polyacrylamide 7-15%. bands revealed by silver staining): samples numbered 1 to 5 are standards for the blind experiments a. c. e, h, m, p. Lane 1. Molecular weight standards for electrophoresis; lane 2, monoclonal IgG added with human serum albumin; lane 3. Tyrode's buffer without human serum albumin; lane 4.1 x 10 2 anti-IgE dilution; lane 5.1 x 10 3 dilution. Samples tested Wind: a and c, buffer; e, 1 x 10 36 anti-IgE dilution: h. 1 x 10 2 anti-IgE dilution; m, 1 x 10 3 anti-IgE dilution; p. 1 x 10 35 anti-IgE dilution. 
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Human basophil degranulation triggered by very dilute antiserum against IgE

June 1988

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5,770 Reads

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488 Citations

Nature

When human polymorphonuclear basophils, a type of white blood cell with antibodies of the immunoglobulin E (IgE) type on its surface, are exposed to anti-IgE antibodies, they release histamine from their intracellular granules and change their staining properties. The latter can be demonstrated at dilutions of anti-IgE that range from 1 x 10(2) to 1 x 10(120); over that range, there are successive peaks of degranulation from 40 to 60% of the basophils, despite the calculated absence of any anti-IgE molecules at the highest dilutions. Since dilutions need to be accompanied by vigorous shaking for the effects to be observed, transmission of the biological information could be related to the molecular organization of water.

Citations (3)


... As indicated by the author Father of homeopathy, Samuel Hahnemann (1755-1843), the course of progression actuates the "essential energy" of the weakened substance, and that progressive weakening's increment the "strength" of the arrangement. Be that as it may, the solutions to the inquiries why and how it happens have not been explained up to this point [21,22] . This is on the grounds that, at such a low weakening, no atom or Nano-particles of the first restorative material is probably going to be available. ...

Reference:

A review on concept of potentization in homoeopathy with physiochemical properties and other molecular structure’
Human basophil degranulation triggered by very dilute antiserum against IgE

Nature

... Important information is hidden in space-time oscillations, and by revealing it we discover that the degree of complexity of such waves is parallel to the system's ability to adapt to perturbations, so that deterministic chaos is welcome in physiology, it promotes efficacy, efficiency, optimality, minimum energy expenditure in the behavior of biosystems, e.g. in microcirculation [2], while complexity is decreasing in pre-pathological and pathological states. This degenerative process of complexity can be observed mathematically and geometrically in the disintegration of the strange attractor [moving toward simpler and more banal equilibria] both from a topological and functional point of view, and similarly also in real observations, for instance of water clusters in the function of the nuclear and mitochondrial genome, and in the interactions between aqueous solutions [3][4][5][6], fields see Montagnier et al, [7]. This degenerative process can be observed both from mathematical-geometric-topological and functional anatomical point of view, and it should be correlated with the structure and function of information fields. ...

Human basophil degranulation triggered by very dilute antiserum against IgE

Nature

... The second direction (discrete dilution/sequential dilution) existing from approximately the end of the 18th century and aimed at obtaining aqueous solutions of ultra-low concentrations caused heated discussions about the reality of the declared effects. Perhaps, at the end of the 20th century, the most resonant research was studying by natural science methods of verification of changes in the physicochemical properties of aqueous solutions with a substance in an ultra-low concentration, the results of which were published in a Nature journal article in 1988 (Davenas et al., 1988). A supposition was made that the observed effects may be caused by the peculiarities of the preparation of aqueous solutions of ultra-low concentrations, involving vigorous shaking, thus leading to the appearance of long-lived molecular structures of water. ...

Human basophil degranulation triggered by very dilute antiserum against IgE

Nature