Isabelle Brunette's research while affiliated with Université du Québec à Montréal and other places

Publications (130)

Article
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Purpose: To evaluate long-term in vivo functionality of corneas regenerated using a cell-free, liquid hydrogel filler (LiQD Cornea) after deep corneal trauma in the feline model. Methods: Two healthy cats underwent 4 mm diameter stepwise 250/450 µm deep surgical corneal ablation with and without needle perforation. The filler comprising 10% (w/w) c...
Article
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SLC4A11 is a H⁺/NH3/water transport protein, of corneal endothelial cells. SLC4A11 mutations cause congenital hereditary endothelial dystrophy and some cases of Fuchs endothelial corneal dystrophy. To probe SLC4A11’s roles, we compared gene expression in RNA from corneas of 17-week-old slc4a11−/− (n = 3) and slc4a11+/+ mice (n = 3) and subjected to...
Conference Paper
Validation of a modified version of an NCCA (NCCAm) and comparison with the Cochet Bonnet Aesthesiometer (CBA) in subjects with normal corneas. The NCCA device described by Murphy et al (1996, 1998) to measure corneal sensitivity was modified as follows: The air reservoir was placed outside the enclosure and the fan inside, and a 3-way solenoid val...
Article
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The long-term survival of biomaterial implants is often hampered by surgery-induced inflammation that can lead to graft failure. Considering that most corneas receiving grafts are either pathological or inflamed before implantation, the risk of rejection is heightened. Here, we show that bioengineered, fully synthetic, and robust corneal implants c...
Article
Fuchs endothelial dystrophy (FECD) is characterized by a progressive loss of corneal endothelial cells (CECs) and an abnormal accumulation of extracellular matrix in Descemet's membrane leading to increased thickness and formation of excrescences called guttae. Extracellular matrix homeostasis is modulated by an equilibrium between matrix metallopr...
Article
Purpose: To compare the sealability of femtosecond laser (FSL) corneal incisions (CIs) with that of triplanar manual (M)-CIs, and to determine FSL wound parameters minimizing leakage. Setting: Private practice. Design: Phase IV, single-surgeon, retrospective cohort study including consecutive cataract surgery cases. Methods: One eye per patient wa...
Article
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Fuchs endothelial corneal dystrophy (FECD) is a degenerative eye disease characterized by corneal endothelial cell (CEC) death and the formation of guttae, an abnormal thickening of CEC’s basement membrane. At the tissue level, an oxidative stress causing mitochondrial damage and CEC death have been described to explain FECD pathogenesis. At the ce...
Article
Fuchs endothelial corneal dystrophy (FECD) is characterized by progressive loss of corneal endothelial cells (CECs) and excessive deposition of extracellular matrix, resulting in Descemet's membrane thickening and formation of excrescences (guttae) on its posterior surface. The role of the endothelium being to keep the cornea dehydrated and transpa...
Chapter
Herpes Simplex Virus-1 (HSV-1) infections in the eye often originate in the cornea before assuming a latent state in the trigeminal ganglion. During primary infection and upon injury or reactivation, HSV-1 can lead to significant corneal damage. Nanoparticles (NPs) are an emerging strategy for drug delivery to the cornea because they improve the lo...
Article
Purpose: To report outcomes of keratopigmentation (KP) with commercial black ink in the treatment of dysphotopsia secondary to laser peripheral iridotomies (LPI) using manual anterior stromal puncture (ASP) and manual lamellar pocket (LP). Methods: This is a retrospective case series of eyes that underwent KP for treatment of dysphotopsia second...
Article
This is a case report of post-laser in situ keratomileusis (LASIK) multidrug-resistant Mycobacterium abscessus keratitis managed with combined topical amikacin and linezolid, flap amputation, and corticosteroids. A 34-year-old woman presented with a corneal interface infiltrate 3 weeks after LASIK. Cultures isolated mycobacteria. The infiltrate did...
Article
Cells and tissues are influenced by environmental conditions. In vivo, the corneal endothelium is subjected to hydrostatic intraocular pressure (IOP) and to the hydrokinetic pressure of the moving aqueous humor in the anterior chamber. In this paper, we used a corneal bioreactor to recreate the IOP condition and investigated the effect of the in vi...
Conference Paper
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This study investigates the possible existence of different natural corneal shape categories. This is important to better describe cornea for both diagnostic and therapeutic assessments. We started by describing corneal shape of different populations as a function of influencing clinical data i.e. age, ametropia and gender. This was done by averagi...
Article
Purpose : Gold-nanoparticle (AuNP) mediated femtosecond laser optoporation transiently permeabilizes the cellular membrane, allowing the introduction of membrane-impermeable molecules, such as DNA plasmids, into targeted cells. Maximizing the specificity of AuNP towards target cells is necessary to enhance the safety of the technique for clinical u...
Article
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Fuchs endothelial corneal dystrophy (FECD) is a corneal pathology that affects the endothelial cell ability to maintain deturgescence, resulting in a progressive loss of corneal transparency. In this study, we investigated the expression of function-related proteins in corneal endothelial cells using FECD or healthy corneal endothelial cells, eithe...
Chapter
Currently, full-thickness transplantation with human donor corneas is the most widely accepted treatment for corneal blindness. However, due to a severe shortage of human donor corneas as well as problems associated with the storage, screening, and immune response to allogeneic tissues, there has been a push to develop alternative therapies and mat...
Conference Paper
L’étude qui sert ici d’exemple en est une dont le but était de comparer la forme 3D de la cornée de patients atteints d’une dystrophie endothéliale de Fuchs opérés selon deux techniques de greffe: Descemet Membrane Endothelial Keratoplasty (DMEK) vs. Descemet Stripping Automated Endothelial Keratoplasty (DSAEK). Cette étude rétrospective visait les...
Chapter
The growing global shortage of corneal tissue for transplantation, in part exacerbated by population aging, has prompted the search for alternatives to native human corneas. Tissue engineering of a corneal endothelium appears to be a quite interesting option. The attractiveness of this approach resides in the possibility of expanding the number of...
Article
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Primary corneal endothelial cell cultures and 3D engineered tissue models were used to study the aberrant deposition of extracellular matrix (ECM) in a vision impairing pathology known as Fuchs endothelial corneal dystrophy (FECD). Corneal endothelial cells (CEC) were isolated from excised Descemet membranes of patients with end-stage FECD. CEC iso...
Article
Corneal blindness is a major cause of blindness in the world and corneal transplantation is the only widely accepted treatment to restore sight in these eyes. However, it is becoming increasingly difficult for eye banks to meet the increasing demand for transplantable tissue, which is in part due to population aging. Donor tissue shortage is theref...
Article
Ocular biometrics refers to the use of features of the eye for person recognition. For instance, the unique and stable texture of the iris has been recognised as a powerful ocular biometric characteristic. In this study, the authors propose to improve biometric authentication with a multimodal ocular biometric system based on the iris pattern and t...
Article
Purpose: Spectral-domain optical coherence tomography (SD-OCT) is widely used in clinical ophthalmology and recently gained popularity in laboratory research involving small rodents. Its noninvasive nature allows repeated measurements, thereby decreasing the number of animals required. However, when used at a conventional dosage, xylazine (an α2-a...
Chapter
The economic evaluation of healthcare interventions is now a prerequisite in many jurisdictions. Adoption of new healthcare interventions cannot only be based on their efficacy and safety. In the context of limited healthcare resources we are facing, their economic impact should also be considered. To estimate the economic impact of health interven...
Article
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Purpose: Fuchs' endothelial corneal dystrophy (FECD), a degenerative disease of the corneal endothelium that leads to vision loss, is a leading cause of corneal transplantation. The cause of this disease is still unknown, but the implication of oxidative stress is strongly suggested. In this study, we analyzed the impact of FECD on mitochondrial D...
Article
In this study, the authors present two new techniques with their own particular advantages dedicated to the authentication of a person based on the three-dimensional geometry of the cornea. A device known as corneal topographer is used for capturing the shape of each cornea. Until now only a few studies on corneal biometry have been conducted and t...
Article
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Aims To determine the influence of prostaglandin analogues (PGAs) on corneal biomechanical properties in patients undergoing chronic treatment for primary open-angle glaucoma (POAG). Methods Prospective, interventional case–control study. 70 eyes from 35 patients with POAG on chronic PGA therapy were recruited. One eye per patient underwent PGA ces...
Article
Purpose: The current and projected shortage of transplantable human donor corneas has prompted the development of long-term alternatives to human donor tissue for corneal replacement. The biosynthetic stromal substitutes (BSS) characterized herein represent a potentially safe alternative to donor organ transplantation for anterior corneal stromal...
Article
The cornea is the transparent front part of the eye that transmits light to the back of the eye to generate vision. Loss of corneal transparency, if irreversible, leads to severe vision loss or blindness. For decades, corneal transplantation using human donor corneas has been the only option for treating corneal blindness. Despite recent improvemen...
Article
Full-text available
Purpose: To evaluate the functionality of a corneal endothelium reconstituted by injection of corneal endothelial cells (CEC) in the anterior chamber of a feline model. Methods: We operated the right eyes of 16 animals. Eight underwent central endothelial scraping and injection with 2 × 105 (n = 4) or 1 × 106 (n = 4) feline CEC supplemented with...
Article
In medical imaging, it is now common to create 3D models of organs by ‘averaging’ several specimens obtained from different subjects. This requires a registration step to align the organs before averaging their shapes. In this paper, we present the difficult case of a quasi-spherical organ: the cornea. To cope with the lack of anatomical anchor poi...
Article
To characterize the three-dimensional (3D) shape, volume distribution, and mirror symmetry of the right and left corneas at the scale of a large population, based on the integrated analysis of 3D corneal shape average maps and topography parameters. A total of 7670 Orbscan II corneal topographies from 3835 consenting subjects with no history of ocu...
Article
The goal of this study was to determine the changes in surgical techniques and leading indications for corneal transplantations performed in the last decade. The impact of administrative changes of corneal banking in Quebec was also evaluated. The records of all corneal transplantations performed between January 2000 and December 2011 in the territ...
Article
Three-dimensional tissues, such as the cornea, are now being engineered as substitutes for the rehabilitation of vision in patients with blinding corneal diseases. Engineering of tissues for translational purposes requires a non-invasive monitoring to control the quality of the resulting biomaterial. Unfortunately, most current methods still imply...
Article
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The purpose of this study is to authenticate a surface from a set of surfaces based on 3D acquisitions in the context of corneal biometric application. The key idea is to quantify the difference between surfaces, and to define a threshold to determine if compared surfaces belong to the same person. This threshold depends on the normal variation bet...
Article
There is a need for an alternative to human donor corneas as the availability of good-quality tissues remains limited, with this situation potentially worsening as the population in many countries is progressively ageing. There have been numerous attempts to develop corneal equivalent as alternatives to donated human corneas as well as prostheses....
Article
Purpose: Corneal tissue shortage has become a major concern worldwide, which has motivated the search for alternative solutions to eye bank human eyes for corneal transplantation. Minimally invasive lamellar transplantation and tissue engineering may offer new opportunities for the rehabilitation of diseased corneas. The aim of this study was to e...
Conference Paper
The only currently available treatment for irreversible corneal endothelial failure is allo-transplantation, which is overshadowed by optical distortion of the posterior surface, postoperative donor corneal endothelial cells attrition and risk of rejection. Assessment of the in vivo functionality of a corneal endothelium reconstituted by the inject...
Article
In this study we present a method to build 3D corneal atlases of different populations using a registration step based on inter-surface volume minimization. First the construction method is presented. It is based on a global factor computation in order to minimize the volume between several surfaces. Then the significance of the choice of the match...
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The goal of this study was to assess the effect of corneal hydration on the quality of the femtosecond laser (FSL) anterior lamellar cut. The Visumax FSL was used to dissect an 8-mm-diameter corneal flap in 22 eye bank corneas showing various levels of hydration. The intended ablation depth was 220 µm in all eyes, which corresponded to the maximal...
Conference Paper
Purpose: To assess the in vivo functionality of a corneal endothelium reconstituted by injection of corneal endothelial cells (CEC) in the anterior chamber (AC) of a feline endothelial deficiency model. Methods: The central corneal endothelium of one eye was scraped off and human (2 x 106/0.2 mL; n = 1 animal) or feline (1 x 107/0.2 mL; n = 2) cul...
Article
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We developed cell-free implants, comprising carbodiimide crosslinked recombinant human collagen (RHC), to enable corneal regeneration by endogenous cell recruitment, to address the worldwide shortage of donor corneas. Patients were grafted with RHC implants. Over four years, the regenerated neo-corneas were stably integrated without rejection, with...
Article
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Corneal grafts for Descemet's Stripping Automated Endothelial Keratoplasty are commonly prepared using mechanical microkeratomes. However, the cuts produced in such way render corneal lenticules that are thinner centrally than peripherally, thus inducing a hyperopic shift. Here we describe a novel device for preparing donor corneal grafts, in which...
Conference Paper
In this study, we present an algorithm to build a mean surface, applied to the human cornea, for the study of variability within a population. Due to the smoothness of the corneal surface, there is no anatomical anchor. The main challenge is to match several surfaces from different subjects to build the mean cornea. The key idea is to use a registr...
Article
PURPOSE: To assess the accuracy of the scanning electron microscopy (SEM) and present alternative approaches to quantify surface roughness based on numerical analysis. SETTING: Department of Ophthalmology, Maisonneuve-Rosemont Hospital, University of Montreal, Montreal, Quebec, Canada. DESIGN: Experimental study. METHODS: Lamellar stromal cuts were...
Article
Purpose: We evaluated the in vivo functionality of a corneal endothelium tissue-engineered using corneal endothelial cells from human patients with Fuchs endothelial corneal dystrophy (FECD). Methods: A total of 15 healthy cats underwent full-thickness corneal transplantation. All transplants were of xenogeneic human origin and all grafts but tw...
Article
This paper describes a methodology to build and compare 3D models (or atlases) of the cornea for specific populations. Using topography data of the anterior and posterior corneal surfaces, average and statistical variation maps are computed after registration of individual corneas on a reference sphere. With this methodology, a normal population mo...
Article
Scanning Electron Microscopy (SEM) is widely used in science to characterize the surface roughness of materials. Three-dimensional information can be obtained with SEM based on stereovision techniques. A stereo pair is typically obtained by tilting the sample by a few degrees. In this paper we present a fully automated method for 3D reconstruction...
Conference Paper
Purpose: : To assess the feasibility of culturing, without viral transduction, human corneal endothelial cells from patients with Fuchs endothelial dystrophy (FECD). We also evaluated which conditions yielded the best results in culture. Methods: : Descemet’s membranes (DM) excised from 29 consenting patients undergoing Descemet stripping automate...
Article
The purpose of this study was to assess the feasibility of initiating primary cultures of corneal endothelial cells from patients suffering from Fuchs endothelial corneal dystrophy (FECD; MIM# 1036800). We also evaluated which conditions yielded the best results for culture. Twenty-nine patients undergoing Descemet stripping automated endothelial k...
Article
Corneal transplantation is the only available treatment to restore vision in patients suffering from endotheliopathies. Tissue engineering offers new alternatives for the replacement of dysfunctional corneal endothelial cells. This review reports the current knowledge on the reconstruction and transplantation of a tissue-engineered corneal endothel...
Article
The goal of this study was to report on the advantages and limitations of the pig and feline models for experimental in vivo corneal transplantation. Ten healthy domestic pigs and ten healthy cats were used. Full thickness penetrating keratoplasty was performed using autologous (eight cases), allogeneic (seven cases) or human xenogeneic (three case...
Article
To characterize the 3-D corneal shape deformation incurred by Fuchs corneal dystrophy and pseudophakic bullous keratopathy by using the integrated analysis of Orbscan (Bausch & Lomb Surgical, Rochester, NY) topographic maps of affected and normal corneas. One hundred thirty-seven patients with Fuchs dystrophy or pseudophakic keratopathy were divide...
Article
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We report measurements of crater diameter and surface ablation threshold as a function of laser fluence in porcine corneal stroma and fused silica with pulse durations of 7 fs (2.7 optical cycles), 30 fs and 100 fs at 800 nm. For laser pulses with Gaussian radial intensity profile, we show experimentally that the square of the crater diameter is a...
Article
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The authors conducted in vivo assessment of corneal endothelial toxicity of air and SF6 in the feline model. This research was motivated by the increased use of air in anterior segment surgery in human subjects. This was a prospective masked study. The eyes of 16 healthy adult cats were randomly assigned for the injection of 0.7 mL air into the ant...
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To assess the cost-utility of posterior lamellar keratoplasty (PLK) techniques, including deep lamellar endothelial keratoplasty, Descemet stripping endothelial keratoplasty, and Descemet stripping automated endothelial keratoplasty, in the treatment of corneal endothelial diseases. Cost-utility analysis based on a Markov model. Cohort of 100 patie...