I. O. Oliinyk’s research while affiliated with Institute of Pediatrics Ob&Gyn NAMS of Ukraine and other places

The article provides data on the impact of chronic stress and changes in the conditions of providing medical care under martial law on the occurrence of chronic diseases, including skin, namely pemphigus. Purpose:to show the peculiarities of the course of pemphigus vulgaris and the management of a patient with this pathology in the condition of martial law. Results.A clinical case of a patient with pemphigus vulgaris is given. Requirements for conditions of maintenance therapy for patients with severe chronic dermatoses.

The article provides data on the etiopathogenetic links of Mycosis fungoides of the skin. A clinical case of a patient with Mycosis fungoides of the skin, which developed under martial law, is presented. Recommendations for examination and treatment of such patients are presented.

The article presents experimental data on the study of the ability to form biofilms of clinical strains of bacteria isolated from combat wounds. The search results regarding the determination of MBС dioxidin in relation to clinical strains – representatives of different taxonomic groups are provided. the dynamics of formation of resistance to dioxidin was determined using the reference strains S. aureus ATCC25923, P. aeruginosa ATCC27853. The purpose evaluation of the ability to form biofilms of dominant representatives of the microbiocenosis of combat wounds and the effect of dioxidin on their expression level Materials and methods. In general, the ability to form biofilms of 20 clinical strains of pathogens was determined (S. haemolyticus – 8 samples; K. pneumoniae – 4 samples; E. coli, P. aeruginosa and S epidermidis – 2 samples each and C. amalonaticus and S.aureus – each one) and 3 reference cultures: E. сoli ATCC25922, S. aureus ATCC25923, P. aeruginosa ATCC27853 and the effect on their formation of dioxidin solution with determination of MBK against planktonic cells of these strains Research results: It was established that both reference and clinical strains of microorganisms were characterized by medium and high ability to form biofilms. According to the results of the conducted studies, it was shown that the drug dioxydin showed antimicrobial activity against the majority of planktonic cells of all gram-negative bacteria used in the work, with an MBC from 0.04 to 0.63 mg/ml. The lowest level of activity of the drug was noted against strains of staphylococci (MBC from 1.25 to >2.5 mg/ml), except for planktonic cells of strain S. aureus № . 2093, for which the MBC of dioxidin was 5 mg/ml. In the course of the study, an analysis of the dynamics of the formation of resistance to dioxidin in microorganisms of different taxonomic groups was carried out. S. aureus ATCC25923 strain formed a variant resistant to dioxidin (MBK ≥ 10 mg/ml), while P. aeruginosa ATCC27853 strains did not form such variants, which indicates the possibility of using dioxidin in the long-term therapy of wound infection. Conclusion. The obtained research results demonstrate the microbiological effectiveness of dioxidin, which can be used in the complex treatment of microbial complications of wound injuries

Considering the numerous clinical manifestations of herpes infection, the long recurrent course of the disease and the lifelong persistence of the virus in the body, its treatment is difficult and not always effective. Now none of the known antiviral drugs is able to eliminate the virus from the human body. The goal – to increase the effectiveness of therapy for patients with herpes virus infection by using an antiviral cream containing menthol. Materials and methods. 33 people suffering from herpes virus infection were under observation. Of these, 19 people had herpes zoster, 14 had HSV, 8 had herpes labialis, and 6 had herpes nasofacialis. The age of the patients ranged from 21 to 69 years. There were 20 women, 13 men. The patients were divided into clinical and therapeutic groups representative of age, gender, and nosological forms. The 1st experimental group included 19 patients, of which 1–9 had herpes simplex virus and 10 – herpes variocele zoster. The 2nd comparison group included 14 people, 5–5 had HSV-1 and 9 had herpes variocele zoster. Patients with herpes zoster received systemic antiviral drugs in complex therapy. Patients with HSV-1 in group I used lipster mint cream topically, patients in group II – cream with acyclovir. Creams were applied 5 times a day in a thin layer to the affected areas. Results. Subjective signs of itching, pain, and burning regressed after the first application of an antiviral cream containing menthol in patients of the 1st group. In the prodromal period 7 patients with HSV group I had an abortive form of herpes infection. In patients of the II group the decrease in subjective sensations was noted on the 2nd-3rd day of treatment. In patients with herpes zoster in both the I and II groups the dynamics of disease regression was equivalent. In patients of the 1st group the subjective feeling significantly decreased after applying the drug, which lasted for 30–40 minutes, and significantly stopped on the 3rd-4th day of treatment. Conclusions. Thus, the use of an antiviral cream containing menthol increases the effectiveness of the therapy of herpes virus infection caused by HSV and herpes zoster, due to the reduction of subjective manifestations: reduction of pain, itching, burning already at the first applications, which improves the quality of life. The use of this agent in the prodromal period prevents the appearance of extensive clinical manifestations of the disease, contributing to the development of abortive forms of herpes virus infection. The cream ensures rapid positive dynamics of the elements and prevents the development of secondary infection.

The work provides justification for the need to improve the diagnosing methods of the life activity of fungi in the sanogenesis process of onychomycosis. The proprietary technique based on the detection of complementary DNA by reverse transcription of the polymerase chain reaction is proposed. The algorithm for evaluating the elimination of onychomycosis pathogens has been given.

The case of generalized local scleroderma with the positive effect as a result of the therapy is described. The goal is to evaluate the therapeutic effectiveness of complex treatment using antifibrotic agents in a clinical case of generalized focal scleroderma.

The results of preliminary observations indicate that the SARS-COV-2 virus can also affect the skin and affect the course of chronic diseases, including dermatoses. The cause and pathogenesis of skin manifestations have not yet been established and are under study, but they are most likely caused by infectious and allergic damage to blood vessels, as well as a high level of pro-inflammatory cytokines. The subject is to increase the effectiveness of the therapy of patients with psoriasis after suffering from the coronavirus disease by using drugs that affect the state of the endothelium of blood vessels and the blood coagulation system. Materials and methods. 69 patients with psoriasis associated with COVID-19 infection were under observation. The patients were divided into two clinical and therapeutic groups, which were representative in terms of age, sex, and severity of the disease: The first experimental group (36 patients) in complex treatment received drugs that had an effect on the normalization of endothelial dysfunction and the blood coagulation system. Patients of the II group (33 persons) received treatment according to the protocols. The control group consisted of 20 practically healthy persons, representative in terms of age and sex. Control of the treatment was carried out on the basis of clinical and laboratory data. Subjective and objective signs of the disease were evaluated, clinical and biochemical indicators were determined, including a general blood test, the state of the coagulation system using modern standardized laboratory tests. The degree of inflammatory reaction was assessed by the level of C-reactive protein and IL-6. Statistical processing of data was carried out using methods of variational statistics. Significance of differences was assessed using Student’s test for independent samples. The results. When using drugs that had an effect on the normalization of endothelial dysfunction and the blood coagulation system, in the complex treatment of patients of the 1st group, a significant improvement in the general condition was noted, a decrease in the subjective symptoms of the disease: itching, dryness, soreness in the foci of inflammation, which decreased 3–4 days earlier, than in patients from the comparison group who received traditional therapy. Clinical remission in patients of the I group occurred in (22.3 ± 2.7) days, while in patients in the II group – in (29.2 ± 3.5) days, that is, 6.9 days earlier (p < 0, 05). The use of the proposed treatment-prophylactic approach improved the parameters of the blood coagulation system in patients with psoriasis who suffered a coronavirus infection, which was manifested by the normalization of AChT, PTI, fibrinogen content and a significant decrease in D-dimer, which indicates a probable reduction in the risk of thrombotic complications. At the same time, there was a significant decrease in inflammation indicators – PSA and IL-6. Conclusions. The use of the proposed treatment-prophylactic approach contributed to an earlier onset of clinical remission: the subjective and objective signs of the disease stopped almost 7 days earlier than in patients treated only by traditional methods, normalization of indicators of the state of the coagulation system, endothelial dysfunction, which prevented development of complications from the cardiovascular system.

The article provides data on the study of blood circulation disorders and microcirculation of the limbs in patients with onychomycosis by the method of thermal imaging, which is non-invasive and allows non-contact monitoring and treatment at all stages of the disease’s sanogenesis. The method of infrared thermography made it possible to confirm the presence of concomitant pathology and reveal the degree of blood circulation and microcirculation disorders in patients with onychomycosis, which was the basis for prescribing vascular and anti-inflammatory therapy to these patients.

The objective. Raise physician awareness to prevent the development of photosensitization in patients with COVID-19. Materials and methods. A review of the literature and analysis of clinical cases of photosensitization associated with SARS-CoV-2- infection and its treatment carried out. Results. Based on the literature and clinical experience, the article presents the possible causes of manifestations and exacerbations of photoallergic contact dermatitis, presents clinical cases of photosensitization associated with coronavirus infection. Conclusions. The issue of protection against ultraviolet radiation is relevant in the summer, especially during a pandemic associated with the emergence of a new coronavirus infection.

The objective. Decrease the number of diagnostic mistakes of pemphigus vulgaris and late forms of syphilis to improve quality of life and prevent premature death in patients with pemphigus vulgaris. Raise doctors’ awareness of the use of treponemal tests to clarify the diagnosis of late forms of syphilis. Materials and methods. The case of pemphigus vulgaris determined on the basis of clinical picture, anamnesis, cytological examination results is described. A concomitant pathology was diagnosed, i.e., late syphilis due to the complex of serological tests: nontreponemal and treponemal (Venereal Disease Research Laboratory test (VDRL), Enzyme-Linked Immuno Sorbent Assay (ELISA), T. pallidum Haemagglutination Assay (TP-HA), Fluorescent Treponemal Antibody Absorption test (FTA abs), ELISA-Different). Results. At dynamic supervision and inspection of the patient on pemphigus vulgaris and late syphilis on the basis of positive treponemal tests was revealed: ELISA sums. (IgG+IgM) – positive, high-quality TP-HA «4+» – positive; semi-quantitative TP-HA 1: 8 «2+» – positive; FTA abs «3+» – positive. To verify the diagnosis, a serum sample was examined by ELISA-Different method. Conclusions. Due to a wide range of serological research and consultation of related specialists, late syphilis was diagnosed in a pemphigus vulgaris patient.