January 2025
·
14 Reads
Science Bulletin
This page lists works of an author who doesn't have a ResearchGate profile or hasn't added the works to their profile yet. It is automatically generated from public (personal) data to further our legitimate goal of comprehensive and accurate scientific recordkeeping. If you are this author and want this page removed, please let us know.
January 2025
·
14 Reads
Science Bulletin
January 2025
·
6 Reads
Theranostics
Rationale: Fecal microbiota transplantation (FMT) is advantageous for treating intractable diseases via the microbiota-gut-organ axis. However, invasive administration of gut microbiota via nasal feeding tubes limits the widespread application of FMT. Here, we attempted to develop a novel strategy to deliver gut microbiota using nanocapsules. Methods: Single-cell nanocapsules were fabricated within 1 h by layer-by-layer assembly of silk fibroin and phosphatidylcholine to generate a protective nanoshell on the cell surface of complicated microbiota. The physical properties of the microbiota nanocapsules were analyzed. The protective effects of nanocapsules on the gastrointestinal tract were analyzed both in vitro and in vivo. The efficacy of FMT assisted by single-cell nanocapsules (NanoFMT) was evaluated using the inflammatory response, gut microbiota balance, and histopathological analysis in animal model. Results: The nanocapsules achieved a good coating ratio for a single type of microbe and complex microbiota, resulting in a remarkable increase in the survival rate of microbes in the gastrointestinal tract. NanoFMT improved the diversity and abundance of the gut microbiota better than common FMT in germ-free mice. Moreover, NanoFMT alleviated intestinal inflammation and positively reversed the microbiota balance in a mouse model of colitis compared with common FMT, assisted by the inherent anti-inflammatory effects of silk fibroin and phosphatidylcholine. Conclusions: Considering its rapid preparation, convenient delivery, and perfect therapeutic effect, we anticipate that NanoFMT may be a promising clinical candidate for next-generation FMT treatment.
January 2025
iScience
October 2024
·
14 Reads
Colorectal cancer is the second most common cause of cancer mortality worldwide, and liver metastasis is the major cause of death in patients with colorectal cancer. Dysfunctional E3 ligase activity has recently been shown to be associated with colorectal cancer. However, the key E3 ligase affecting colorectal cancer liver metastasis remains unknown. Therefore, we used an shRNA library targeting 156 E3 ubiquitin ligases to perform an in vivo loss-of-function screen of a human colorectal cancer cell line in a mouse model of colorectal cancer liver metastasis. The screen revealed that NEDD4L knockdown promoted colorectal cancer liver metastasis. Moreover, overexpression of NEDD4L prevented colorectal cancer liver metastasis. Mechanistic studies revealed that NEDD4L bound to the PPNAY motif in PRMT5 and ubiquitinated PRMT5 to promote its degradation. PRMT5 degradation attenuated the methylation of an arginine residue in AKT1 to inhibit the AKT/mTOR signaling pathway, consequently decreasing colorectal cancer cell proliferation. We are the first to show that PRMT5 is a substrate protein of the E3 ligase NEDD4L and reveals not only the metastasis-inhibiting function of NEDD4L but also a novel mechanism by which NEDD4L suppresses colorectal cancer liver metastasis. These findings may provide a new preventive strategy for liver metastasis.
October 2024
·
26 Reads
October 2024
·
5 Reads
Nano Letters
September 2024
·
1 Read
Medical Gas Research
September 2024
·
10 Reads
·
2 Citations
Chemical Engineering Journal
September 2024
·
81 Reads
·
6 Citations
Cancer Cell
March 2024
·
108 Reads
·
4 Citations
Disrupted gastrointestinal (GI) motility is highly prevalent in patients with inflammatory bowel disease (IBD), but its potential causative role remains unknown. Herein, the role and the mechanism of impaired GI motility in colitis pathogenesis are investigated. Increased colonic mucosal inflammation is found in patients with chronic constipation (CC). Mice with GI dysmotility induced by genetic mutation or chemical insult exhibit increased susceptibility to colitis, dependent on the gut microbiota. GI dysmotility markedly decreases the abundance of Lactobacillus animlalis and increases the abundance of Akkermansia muciniphila. The reduction in L. animlalis, leads to the accumulation of linoleic acid due to compromised conversion to conjugated linoleic acid. The accumulation of linoleic acid inhibits Treg cell differentiation and increases colitis susceptibility via inducing macrophage infiltration and proinflammatory cytokine expression in macrophage. Lactobacillus and A. muciniphila abnormalities are also observed in CC and IBD patients, and mice receiving fecal microbiota from CC patients displayed an increased susceptibility to colitis. These findings suggest that GI dysmotility predisposes host to colitis development by modulating the composition of microbiota and facilitating linoleic acid accumulation. Targeted modulation of microbiota and linoleic acid metabolism may be promising to protect patients with motility disorder from intestinal inflammation.
... In addition to affecting chemotherapy response, F. nucleatum also plays a role in modulating the response to immunotherapy [2]. F. nucleatum promotes an immunosuppressive milieu by interacting with both tumor cells and immune cells, hindering the effectiveness of immunotherapies such as checkpoint inhibitors (e.g., PD-1/PD-L1 inhibitors), CAR T-cell therapies, and other immune modulators [96][97][98][99][100]. As mentioned earlier, F. nucleatum's role in immune modulation is multifaceted. ...
September 2024
Cancer Cell
... This study showed that succinate supplementation upregulated the levels of 13(S)-HPODE and 5(S)-HETE in fatty acyls. 13(S)-HPODE is involved in linoleic acid metabolism and leads to a reduction in intestinal inflammation and changes in the body weight of mice (Zhang, et al., 2024). Moreover, 13(S)-HPODE is a natural activator of the cancer therapeutic target PPARg, which can effectively promote apoptosis of cancer cells in the body (Biswas, et al., 2023). ...
March 2024
... Given the serious threat cancer poses to human health, exploring effective cancer treatment technologies has gained significant interest. Among the many advances made in the past decade, photodynamic therapy (PDT) has emerged as a promising treatment approach due to its ability to generate cytotoxic reactive oxygen species (ROS) by photosensitizers (PSs) under light irradiation [1][2][3][4][5][6]. PDT offers advantages such as minimal invasiveness, high spatiotemporal controllability, and reduced drug resistance, making it a highly effective strategy for cancer treatment with significant clinical potential [7][8][9][10][11]. ...
February 2024
Science Bulletin
... Some known bacteria species with pro-tumoral properties are Fusobacterium nucleatum, Helicobacter pylori, Escherichia coli (certain strains), Salmonella Typhimurium (certain strains), and Enterococcus faecalis. [32][33][34] Fusobacterium nucleatum demonstrates a close association with colorectal cancer (CRC), and it promotes cancer by several mechanisms. The bacterial protein Fap2 interacts with the immune checkpoint receptor TIGIT on NK cells and T cells, inhibiting their antitumor activity. ...
February 2024
... Nutritional support is a critical aspect of perioperative care for patients undergoing major surgeries, such as those for cancer. The early achievement of energy targets after major abdominal surgery has proven to be crucial for patients' quicker postoperative recovery, for example by lowering the risk of infectious complications [29]. Boelens et al. (2014) demonstrated that early postoperative enteral nutrition significantly reduces the duration of postoperative ileus, anastomotic leakage, and hospital stay length [ Figure 3]. ...
February 2024
American Journal of Clinical Nutrition
... In this context, the gut microbiota has emerged as an invaluable source of inspiration for the development of biomimetic solutions in relevant fields such as medicine or biotechnology, among others. Biological principles derived from the gut microbiota, such as resilience, self-regulation, and microbial communication, offer innovative perspectives for the creation of advanced technologies that mimic these natural mechanisms [86][87][88]. ...
January 2024
Bioactive Materials
... Future research should focus on enhancing outcomes for patients with ACM by modulating GM through interventions such as probiotics or dietary modifications. This strategy represents a novel therapeutic approach with significant potential for clinical applications (186,187). ...
Reference:
Gut microbiota and atrial cardiomyopathy
February 2024
hLife
... G6PDH supports GPX4's antioxidant function by generating NADPH, thereby protecting cells from oxidative stress and ferroptosis. Ferroptosis plays a significant role in various diseases, including cancer, neurodegenerative diseases, and ischemia-reperfusion injury [60][61][62]. Numerous studies have proposed new therapeutic strategies targeting the mechanisms of ferroptosis, such as inhibiting transferrin receptors or promoting the expression and function of ferritin to reduce intracellular iron levels, thereby decreasing ferroptosis [63]. Using lipid peroxidase inhibitors or antioxidants can reduce the generation of lipid peroxides and protect the integrity of cell membranes [64]. ...
January 2024
Nature
... This interaction may explain the higher metabolic rate observed in Kv1.3 KO mice(73). Interestingly, the Kv1.3 channel C-terminus also interacts with LAMTOR1, an activator of the mTOR pathway(74). In physiological conditions, it would be interesting to explore how these interactions are balanced within the cell to ensure proper cellular function. ...
January 2024
... The resulting hypoxia can induce CAF-like features in normal fibroblasts [96], and the resulting upregulation of HIF-1α in cancer cells contributes to cancer cell survival following radiotherapy [97,98]. As radiation-induced ECM changes by CAFs also impair the permeation of possible neoadjuvant chemoand/or immunotherapies, CAFs could not only contribute to radioresistance, but also to resistance against possible adjuvant therapy [99,100]. ...
December 2023