Hsing-Yi Chung's research while affiliated with Tri-Service General Hospital and other places

Publications (19)

Article
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Purpose Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a major healthcare threat worldwide. Since it was first identified in November 2021, the Omicron (B.1.1.529) variant of SARS-CoV-2 has evolved into several lineages, including BA.1, BA.2–BA.4, and BA.5. SARS-CoV-2 variants might i...
Article
Full-text available
Objectives To rapid detect for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and immediately distinguish whether positive samples represent variants of concern (VOCs), we established a novel 5-in-1 VOC assay. Methods This assay could distinguish among five VOCs: Alpha, Beta, Gamma, Delta, and Omicron, in a single reaction tube....
Article
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Objectives Since April 2022, another wave of the Omicron epidemic stroke Taiwan society, and children with severe neurological complications have been reported frequently. A few cases even developed acute fulminant encephalitis. To investigate the possible causes of the increased incidence of such complications here in Taiwan, we reviewed several c...
Article
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Since the late 2020, the evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern has been characterized by the emergence of spike protein mutations, and these variants have become dominant worldwide. The gold standard SARS-CoV-2 diagnosis protocol requires two complex processes, namely, RNA extraction and real-...
Article
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COVID-19 has spread globally. SARS-CoV-2 variants of concern (VOCs) are leading the next waves of the COVID-19 pandemic.
Article
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Purpose: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent behind coronavirus disease-2019 (COVID-19). Single-plex reverse transcription-polymerase chain reaction (RT-PCR)-based assays are widely used for COVID-19 detection but exhibit decreased sensitivity and specificity in detecting the rapidly spreading SARS-C...
Article
Since the Coronavirus 19 (COVID-19) pandemic, several SARS-CoV-2 variants of concern (SARS-CoV-2 VOC) have been reported. The B.1.1.7 variant has been associated with increased mortality and transmission risk. Furthermore, cluster and possible co-infection cases could occur in the next influenza season or COVID-19 pandemic wave, warranting efficien...
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Objectives : With the emergence of the B.1.1.7 lineage in the ongoing COVID-19 pandemic, Taiwan confronted a COVID-19 flare up in May 2021. Large-scale, accurate, affordable, and rapid diagnostic tests such as the lateral flow assay can help prevent community transmission, but their performance characteristics in real-world conditions and relevant...
Article
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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic. Additionally, several SARS-CoV-2 variants have continuously challenged diagnostic testing due to viral diversity in spike and nucleocapsid (N) protein mutations. SARS-CoV-2-variant proliferation potentially affects N protein-targeted rapid antigen testing. We perfor...
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Background There is a global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Information on viral genomics is crucial for understanding global dispersion and for providing insight into viral pathogenicity and transmission. Here, we characterized the SARS-CoV-2 genomes isolated from five travelers who returned to Taiwan fro...
Article
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Background/Purpose Mass screening for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is important to prevent the spread of coronavirus disease 2019 (COVID-19). Pooling samples can increase the number of tests processed. LabTurbo AIO 48 is an automated platform that allows ribonucleic acid extraction and sample analysis on the same ins...
Article
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Purpose: Accurate molecular diagnostic assays for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, are needed for epidemiology studies and to support infection-control measures. We evaluated the analytical sensitivity and clinical performance of three sample-to-answer molecular-diagnostics sy...
Article
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The COVID-19 pandemic has caused a global public health crisis. Taiwan experienced two waves of imported cases of coronavirus disease 2019 (COVID-19), first from China in January to late February, 2020 then from other countries starting in early March. As of Dec 14, 2020, 733 cases have been reported in Taiwan, with cases of entire families being i...
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Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has spread rapidly, causing deaths worldwide. In this study, we evaluated the performance of the BD MAX Open System module for identifying viral pathogens, including SARS-CoV-2, in nasopharyngeal specimens from individuals with symptoms of upper respiratory tract infection. We developed a...
Article
Background and aims Immediate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for preventing the spread of coronavirus disease 2019 (COVID-19). The LabTurbo AIO 48 system is an automated platform that allows nucleic acid extraction and sample analysis on the same instrument, producing faster results without aff...
Article
Full-text available
Coronavirus disease 2019 has become a worldwide pandemic. By April 7, 2020, approximately 1,279,722 confirmed cases were reported worldwide including those in Asia, European Region, African Region and Region of the Americas. Rapid and accurate detection of Severe Acute Respiratory Syndrome Virus 2 (SARS-CoV-2) is critical for patient care and imple...
Article
Full-text available
Background: MDR Elizabethkingia anophelis strains are implicated in an increasing number of healthcare-associated infections worldwide, including a recent cluster of E. anophelis infections in the Midwestern USA associated with significant morbidity and mortality. However, there is minimal information on the antimicrobial susceptibilities of E. an...
Article
Full-text available
Elizabethkingia species are ubiquitous bacteria that uncommonly cause human infection. Elizabethkingia anophelis was first identified in 2011 from the mosquito Anopheles gambiae. The currently available bacterial typing systems vary greatly with respect to labour, cost, reliability, and ability to discriminate among bacterial strains. Polymerase ch...

Citations

... 46 The cerebrospinal fluid samples from COVID-19 patients with severe neurological symptoms were generally tested for SARS-CoV-2 RNA negative. 47,57 The above observations suggest that neurological symptoms are unlikely to be driven by direct neural invasion. Certain genetic variants of antioxidant enzymes, including GST family and NRF2, were identified to affect the development of long COVID neurological sequelae. ...
... Therefore, RT-PCR detection technology needs to be constantly optimized to cope with the continuous mutating SARS-Cov-2. The Chung et al. group developed a multiplex RT-PCR by using specific primers or probes targeting nine mutations (ΔHV 69/70, K417T, K417N, L452R, E484K, E484Q, N501Y, P681H, and P681R) on the S gene, and they found that this method exhibited better sensitivity in 250 clinically positive samples than those of the commercial VirSNiP Covid-19 variant kit [11]. Another study showed a multiplex, quantitative, RT-PCR-based molecular beacon technology, the sensitivity and specificity of this technology can achieve 100% in 26 confirmed positive patients [12]. ...
... Viral genomic mutations leading to new variants of SARS-CoV-2 are a real challenge in tackling the global COVID-19 pandemic [6][7][8] . Viruses that are mutated have a high propensity for replication errors, providing them with an advantage; several mutations further occur with each replication cycle, making such viruses more virulent and transmissible [9] . ...
... Due to the similarity of the symptoms of these diseases, with significant differences in further medical management, To perform such a test, a small sample volume is required, a minimum amount of time is spent, and the results there are available in approximately 1 h. However, this requires appropriate equipment dedicated to the reagent cassettes and is generally costly [25]. ...
... While we have modelled hypothetical diagnostic tests with performance consistent with the WHO target product profiles (Organization WH., 2020b), we expect these results to remain qualitatively unchanged among the currently available tests. Indeed, a recent Cochrane review and other empirical studies highlighted that the sensitivity of these tests is lower amongst asymptomatic cases than amongst those with symptoms (Dinnes et al., 2021;Wagenhäuser et al., 2021;Jian et al., 2022;Fernandez-Montero et al., 2021;Döhla et al., 2020), suggesting that LFAs for rapid screening combined with RT-PCR as a confirmatory tool, sequential LFA testing, or our approach of outpatient-based deployment would be favored even more strongly than community-based deployment than modelled here. While our analysis has focused on a population consistent with a South-Asian city, additional analysis showed similar results for a population with age structure consistent with a city in sub-Saharan Africa (see Fig. S5). ...
... The increased use and availability of genomic surveillance tools have linked each COVID-19 wave as originating in different geographical regions caused by new sequence variants. [3][4][5][6][7][8][9][10][11] A designation of a new SARS-CoV-2 variant requires at least one mutation to differentiate it from other related viruses of the same species. New variants are classified as a variant of concern (VOC) if they are associated with demonstrated changes in transmissibility, virulence, or control measures and as a variant of interest (VOI) based on their likelihood to cause changes that can significantly negatively impact public health. ...
... With the development of laboratory technology, pooling samples for the PCR assay is a cost-and time-saving approach. 37 Many endoscopy units could increase their laboratory capacity, improve the feasibility of testing, decrease patient burden, and avoid delays in time-sensitive procedures. 37 Rapid antigen tests can contribute to the overall COVID-19 testing capacity, offering advantages in terms of shorter turnaround times and reduced costs, particularly in situations in which PCR testing capacity is limited. ...
... There is a need for fast and simple detection strategies of SARS-CoV-2 that can be utilized worldwide, especially in developing countries [15,16]. Here, we developed a first-line screening method for SARS-CoV-2 that does not require RNA extraction and can be applied to different variants. ...
... On the other hand, the Cepheid Xpert Xpress SARS-CoV-2 (Xpert Xpress; Cepheid, Inc., Sunnyvale, CA, USA) is a sensitive and specific random access, cartridge-based RT-qPCR, which targets two genes, the N2 region of the nucleocapsid (N) and envelope (E) genes [7]. As of December 2022, comparative evaluations of BioFire RP2.1 and high-throughput SARS-CoV-2 assay platforms or other sample-to-answer SARS-CoV-2 RT-PCR assay [8][9][10][11] using serial dilutions of high-titer positive samples, or reference SARS-CoV-2 RT-qPCR assay [12], have been reported [13,14]. ...
... Ovation RNA-Seq System V2 (Nugen Technologies, San Carlos, CA, USA) was used to synthesize cDNA, which was then processed into a library as described previously [26]. WGS was performed as described previously [27]. Briefly, whole-genome sequences of the SARS-CoV-2 isolates (TSGH-42 and TSGH-43) were obtained following the protocol of the Illumina TruSeq Stranded mRNA Library Prep Kit to enrich SARS-CoV-2 cDNA using multiplex RT-PCR amplicons. ...