Hsin-Tsung Ho's research while affiliated with Mackay Memorial Hospital and other places

Publications (39)

Article
This study investigates the development of membranes for leukocytes depletion from model hydrogel interfaces. We first designed hydrogels from 2-(dimethylamino)ethyl methacrylate (DMAEMA) and poly(ethylene glycol) methyl ether methacrylate (PEGMA) to form poly(DMAEMA-co-PEGMA), and [2-(methacryloyloxy)ethyl]trimethylammonium chloride (TMA) and PEGM...
Article
This work discusses the impact of the charge bias and the hydrophilicity on the human blood compatibility of pseudozwitterionic biomaterial gels. Four series of hydrogels were prepared, all containing negatively-charged 3-sulfopropyl methacrylate (SA), and either acrylamide, N-isopropylacrylamide, 2-dimethylaminoethyl methacrylate (DMAEMA) or [2-(m...
Article
Background and objective: Tuberculosis (TB) risk might be increased in patients with diabetes by factors other than hyperglycaemia, such as dyslipidaemia. Host lipids are essential energy sources used by mycobacteria to persist in a latent TB state. A potential therapy targeting cholesterol catabolism of mycobacteria has been proposed, but the pot...
Article
Statement of significance: The design of new zwitterionic copolymer material is proposed and demonstrated in this study. It was showed that hydrophobicoctadecyl acrylate segments can be introduced in the zwitterioniccarboxybetaine polymer chain with a well-controlled random sequence. Stable, efficient, and effective surface zwitterionization of hy...
Article
Blood transfusion is an important but complex and high-risky clinical procedure. Any error could cause serious injuries to patients. To better assure the procedure safety, we enhancing our home-made blood transfusion platform with new clinic decision support components to assure patient's identity and to inform clinicians of any event in time. So f...
Article
We report a novel biomacromolecular formula for the design of hemocompatible gel interfaces of N-isopropylacrylamide (NIPAAm) and mixed-charge pairs of [2-(Methacryloyloxy)ethyl] trimethylammonium (TMA) and 3-sulfopropyl methacrylate (SA) with overall electrical neutrality. The study stresses on how well-defined compositions of nonionic NIPAAm and...
Article
Full-text available
A surface plasmon resonance sensor for Mycobacterium tuberculosis (MTB) deoxyribonucleic acid (DNA) is developed using repeatable telecommunication wavelength modulation based on optical fiber communications laser wavelength and stability. MTB DNA concentrations of 1 μg/mL and 10 μg/mL were successfully demonstrated to have the same spectral half-w...
Article
Aminopeptidase N (CD13) is an ectoenzyme located in the outer membrane of a variety of cells. Proteomic profiling indicates an increased expression of CD13 in phagocytes during Mycobacterium tuberculosis (M. tuberculosis) infection. The purpose of this study was to investigate the role of CD13 on the internalization and intracellular survival of M....
Article
Full-text available
Background: Human cytomegalovirus (CMV) is a lifelong, often asymptomatic infection, with the virus maintained in latently infected myeloid cells. Patients receiving chemotherapy for cancer are at high risk of reactivation of latent CMV, which may further contribute to host immune exhaustion, ultimately leading to a poor antitumor response. Pre-emp...
Article
In this work, the hemocompatibility of zwitterionic polypropylene (PP) fibrous membranes with varying grafting coverage of poly(sulfobetaine methacrylate) (PSBMA) via plasma-induced surface polymerization were studied. Charge neutrality of PSBMA-grafted layers on PP membrane surfaces was controlled by the low-pressure and atmospheric plasma treatme...
Article
We assessed the characteristics of the new semi-quantitative test paper (Clinitek ATLAS Pro(12)) using random urine samples. Three hundred urine samples were analyzed using either the new test paper, conventional dipsticks, quantitative (P/C ratio), or immunological quantitative methods (A/C ratio). Our study showed that the new test paper is highl...
Article
Retention and accumulation of toxic hydrophobic bile salts within hepatocyte may cause hepatocyte toxicity by inducing apoptosis. This study was designed with the purpose of evaluating the possible effect of antithrombin-III on hepatocyte apoptosis in bile duct ligated rat. The rats were randomized to 3 groups: group 1 (control, C) underwent sham o...
Article
Lactic acidemia is commonly associated with severe diseases in pediatric patients. Quantitation of blood lactate and pyruvate is important for the diagnosis and clinical management. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method using dried blood spots (DBS) was developed and could be used for simultaneous quantification of bloo...
Article
We evaluated VITEK 2 cards (NGNC and AST-GN10) for the accuracy of identification (ID) and antimicrobial susceptibility testing (AST) of non-glucose-fermenting Gram-negative bacilli (NGF-GNB). In a total of 201 strains, 190 strains (94.5%) were correctly identified, seven strains (3.5%)showed low discrimination, four strains (2.0%) had discrepancie...
Article
Anti-"Mi(a)" is one of the most important irregular red blood cell antibodies found in Taiwan. The aim of this study was to investigate whether specific HLA-DRB1 alleles are associated with anti-"Mi(a)" production. A case-control retrospective study was performed on 68 patients showing presence of anti-"Mi(a)" and 219 unrelated control subjects fro...
Article
Autoimmune neutropenia in children is caused by granulocyte-specific autoantibodies. These antibodies react to the patient's own neutrophils but disappear when the neutropenia spontaneously remits. This study reviewed our experience with autoimmune neutropenia in children and investigated possible associations with HLA-DR and HLA-DQ alleles. From 1...
Article
Apoptosis is an important process in a wide variety of different biological systems. In addition to caspases, recently, calpains, another family of proteases, have been found to be involved in apoptosis of many cell systems. This study is designed with the aims to evaluate the possible effect of Z-LLY-FMK (a calpain inhibitor) on intestine apoptosi...
Article
The improvement of sensitivity and differentiation in rapidly identifying a small amount of mycobacteria in sputum has significant implications for reducing tuberculosis transmission. We previously applied the conventional PCR and capillary electrophoresis (CE) to establish the restriction fragment length polymorphism (RFLP) pattern of mycobacteria...
Article
Full-text available
We present a simple and novel assay—employing a universal molecular beacon (MB) in the presence of Hg2+—for the detection of single nucleotide polymorphisms (SNPs) based on Hg2+–DNA complexes inducing a conformational change in the MB. The MB (T7-MB) contains a 19-mer loop and a stem of a pair of seven thymidine (T) bases, a carboxyfluorescein (FAM...
Article
Cholestasis leading to retention and accumulation of toxic hydrophobic bile salts within hepatocytes may cause hepatocyte toxicity by inducing apoptosis. Calpains have been found to be involved in apoptosis of many cell systems. This study is designed with the aim of evaluating the possible effect of Z-LLY-FMK (a calpain inhibitor) on hepatocyte ap...
Article
To assess the role of leukapheresis and cranial irradiation in reducing the incidence of intracranial hemorrhage (ICH) and early death in patients with hyperleukocytic acute myeloid leukemia (AML) and the impact of such treatment on survival. This study retrospectively analyzed the records of 75 patients with hyperleukocytic AML who had a white cel...
Article
To rapidly identify Mycobacterium and Nocardia spp. without costly probes, we had implemented capillary electrophoresis (CE) in polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis to analyze their 65-kDa heat shock protein (hsp65) gene. The PCR-RFLP analysis with CE (PRACE) involved only one restriction enzyme,...
Article
A sample of 10 mM flurbiprofen in methanol (or ethanol) was photoirradiated with sixteen 8 W low-pressure quartz mercury lamps irradiated at 306 nm in a Panchum PR-2000 photochemical reactor. In total, four major photoproducts derived from each sample were observed from the HPLC chromatogram. The photoproducts were separated and their structures el...
Article
Obstructive jaundice may promote bacterial overgrowth and altered intestinal barrier function, with resultant increased translocation of endotoxin to the liver, amd thus may potentiate the phenomenon of hepatocyte apoptosis. Exogenous administration of insulin-like growth factor-I (IGF-I) has been shown to enhance mucosal adaptation after small bow...
Article
A case of tinea corporis with the unusual presentation of concentric erythematous rings on the abdomen is reported. The pathogen was identified as Microsporum gypseum. While a number of unusual lesions have been described in M. gypseum infections, we are unaware of any reports on concentric rings.
Article
Full-text available
To report the efficacy and findings of a large-scale preventive screening program for severe acute respiratory syndrome-associated coronavirus (SARS-CoV) using amplification of the virus from a nasopharyngeal swab (NPS) obtained from the health-care workers (HCWs). A prospective observational study. A medical center in Taiwan. Two hundred thirty HC...
Article
Full-text available
Retention and accumulation of toxic hydrophobic bile salts within hepatocyte may cause hepatocyte toxicity by inducing apoptosis. Apoptosis is a pathway of cell death orchestrated by a family of proteases called caspases. Z-Val-Ala-Asp (OMe)-fluoromethyl ketone (ZVAD-fmk) is a cell-permeable irreversible inhibitor of caspase. The purpose of this st...
Article
In this article, we present a simple and cost-effective method for the determination of amino acids in cerebrospinal fluids (CSF) by using capillary electrophoresis-light-emitting diode-induced fluorescence detection. When using a deactivated capillary filled with 0.6% poly(ethylene oxide) (PEO, M r 6.0 × 10 5) prepared in 10 mM tetraborate (pH 9.3...
Article
Full-text available
PCR-restriction fragment length polymorphism (RFLP) analysis is a nonprobe method for the rapid identification of Mycobacterium species. We demonstrate the separation of DNA or restriction fragments digested from the mycobacterial gene encoding the 65-kDa heat shock protein (hsp65) by capillary electrophoresis (CE). By using a pair of unlabeled pri...
Article
Apoptosis is an important process in a wide variety of biologic systems. Cholestasis, or impaired bile formation, occurs in a wide variety of human liver diseases. Retention and accumulation of toxic hydrophobic bile salts in hepatocytes may cause hepatocyte toxicity by inducing apoptosis. In addition, the translocation of bacteria and endotoxin, w...
Article
Translocation of bacteria and endotoxin has long been documented in obstructive jaundice, and altered intestinal barrier function is considered to be one of the important mechanisms for this phenomenon. Proliferating cell nuclear antigen (PCNA), also known as cyclin, is an auxiliary protein of DNA polymerase-delta, and the level of synthesis correl...
Article
Translocation of bacteria and endotoxtin has long been documented in obstructive jaundice, and altered intestinal barrier function is considered to be one of the important mechanisms for this phenomenon. The regulation of gastrointestinal mucosal response to injury is thus of important clinical as well as biological relevance. Integrins play a crit...
Article
There is a high incidence of perioperative morbidity and mortality in patients with obstructive jaundice due to sepsis. Tumor necrosis factor-a (TNF-alpha) is considered a crucial mediator in inducing and processing the inflammatory cascade. We hypothesize that obstructive jaundice leads to an increased endotoxin-induced TNF-alpha production and th...
Article
Hypothesis: CD44 is an adhesion molecule expressed by neutrophils, lymphocytes and epithelial cells. CD44 is involved in cell-cell and cell-matrix binding. In addition to lymphocyte trafficking, CD44 also plays a crucial role in the maintenance of intestinal villus integrity. We hypothesize that obstructive jaundice may alter CD44 expression in th...
Article
Altered gallbladder motility by progesterone has been recognized as an important factor in the development of gallstones. There are two types of hepatolithiasis, that occurs de novo in the intrahepatic ducts with an intact gallbladder (primary hepatolithiasis) and that which originates in the gallbladder and the stones migrate into the intrahepatic...
Article
In clinical trials, appropriate designs are often chosen to address scientific/medical questions of particular interest to the investigator. For a chosen statistical design, however, standard statistical procedures may not be applicable owing to the nature of the design. In this paper we examine statistical methods for analysis of data collected fr...
Article
In recent years, multinational multicenter trials have become increasingly popular for evaluation of the generalizability of clinical results across different countries. With the implementation of the International Conference on Harmonization (ICH) Good Clinical Practice (GCP), multinational trials have become widely accepted by most regulatory age...

Citations

... Such interrelated adhesion of platelets and WBCs has also been reported to occur on membrane oxygenators and filter membranes [40,41]. Even certain leukodepletion filter membranes developed from hydrogel interfaces having different charge densities have shown a charge biased WBC to platelet adhesion, nevertheless their excellent blood compatibility in vitro [42]. So as a result of these adhesions, it is expected that pores of the membranes might be clogged with cells and proteins. ...
... In addition, CuS NPs generally do not possess the ability to initiatively capture bacteria. Although the strategy via surface modification of CuS NPs by a polymer of quaternary ammonium can realize active adsorption of bacteria [13], the existence of abundant positive charges on the NP surface may lead to the apoptosis and hemolysis of normal cells [18]. Studies have shown that quaternary ammonium salt in water can affect the normal development of animal embryos [19][20][21]. ...
... Therefore, reduction of plasma lipids may not be fully effective at controlling latent TB. In fact, our previous study showed that another lipid-lowering agent, ezetimibe, known to inhibit cholesterol uptake into cells, was associated with a lower risk of latent TB in patients with diabetes [29][30][31]. Our current study shows that fenofibrate not only sustains the availability of intracellular lipids but also enhances dormant genes known to involved in lipid metabolism. ...
... Some of these medical devices have been approved by organizations and are used clinically to improve the quality of life of patients. The results are summarized in Table 1 [75,[122][123][124][125][126][127][128][129][130][131][132][133][134][135][136], and several examples of these studies are described in this section. ...
... 17 In addition to positively and negatively charged surfaces, we have evaluated the synergistic effect of zwitterionic monomers known as antifouling and even antibacterial in combination with the antifouling PVP polymer chains. 39,40 We focused on the use of two different kind of zwitterions that either have the positive charge exposed to the external part of the monomer unit, that is, 2-methacryloyloxyethyl phosphorylcholine (M- Preliminary tests on the bacterial adhesion at short incubation times were further completed. The surfaces resulting from this initial adhesion were incubated into culture media at longer periods (up to 72 h) in order to analyze the effect of the surfaces on bacteria growth. ...
... Ethynamide, refabutin, the amino acid salicylic acid, capreomycin, rifampin, hydrochloride, streptomycin, and ethambutol with; [122] the motivation that these systems are becoming more advanced. [142] DNA genome M.tb 22-mer: 5′-CCA ACT TTG TTG TCA TGC ACC C-3′ [132] DNA genome M.tb 24-mer: 5′-CGGTGGCGTGTTCTTTGTGCAATA-3′ [143] Not in reference 20-mer: 5′-CTCGTCCAGCGCCGCTTCGG-3′ [144] sequence IS-6110 5′-SH-[CH2]6-TTT TTT GTG GCC ATC GTG GAA GCG A-3′ [120] sequence IS-6110 15-mer: 5′-ATCCGGCCACAGCCC-3′ [145] Rpo-B 15-mer: 5′-GATACTTCTATCACC-3′ [101] Not in reference 21-mer: 5′-GGTCTTCGTGGCCGGCGTTCA-3′ [100] DNA genome M.tb 20-mer: 5′-CTCGTCCAGCGCCGCTTCGG-3′ [146] DNA genome M.tb 20-mer: 5′-CTC GTC CAG CGC CGC TTC GG-3′ [147] Not in reference 21-mer: 5′-IACIIICAATCCAIIIC-3′ [148] Not in reference 21-mer: 5′-GGTCTTCGTGGCCGGCGTTCA-3′ [149] Rpo-B 21-mer: 5′-ACCCACAAGCGCCGACTGTTG-3′ [133] ESAT-6 antigen' Genomic region [150,151] Not in reference 21-mer: 5′-GGTCTTCGTGGCCGGCGTTCA-3′ [152] sequence IS-6110 18-mer: 5′-GAGCGTAGGCGTCGGTGA-3′ [98] IFN-γ 37-bp aptamer: 5′-GGG GTT GGT TGT GTT GGG TGT TGT GTC CAA CCC CCC C-3′ [153] DNA genome M.tb 25-mer: 5′-biotin GAC CAA ATA GGT ATC GGC GTG TTC A-3′ [154] Not in reference 15-mer: 5′--GATACTTCTATCACC-3′ [102] ...
... APN serves as an important receptor for several pathogens via its extracellular regions, resulting in their attachment or internalization. These include coronaviruses from several species, the human cytomegalovirus (HCMV) and the bacteria M. tuberculosis and F4 + ETEC [76,[204][205][206][207][208]. What makes this receptor so interesting for targeted vaccine delivery is that upon binding of F4 fimbriae to APN, these fimbriae were transported across the epithelium and induced strong immune responses after oral administration [75][76][77]. ...
... Data quality assurance is of high priority in any clinical research because the quality of the data is a major determinant of the validity of the conclusions drawn. High quality data can be ensured by adherence to Good Clinical Data Management Practice (GCDMP) [1] although there is no consensus on what should be regarded as guidelines for GCDMP for all the fields of healthcare research [2]. Generally, it is believed that the information on regulations and guidelines for GCDMP can be obtained from the International Conference on Harmonisation (ICH), Food Drug Agency (FDA) and Society for Good Clinical Data Management [3][4][5] to inform formulation of Standard Operating Procedures (SOPs) for data collection. ...
... It has been suggested that GCV may alleviate T-cell exhaustion in vitro. 36 However, incubation of isolated T cells with GCV did not change levels of PD-1 (Supplementary Figure S2A, B), and treatment of GL261-bearing mice with GCV alone had no effect on animal survival; combination with anti-PD-1 led to 1/6 long-term survivors, compared with anti-PD-1 alone, which led to 2/6 long-term survivors (Supplementary Figure S2C). Thus there was no independent effect of GCV in our models. ...
... Almost any gas can be brought into a plasma state if it is given enough energy Plasma is a combination of electrons, ions, and exciting species, including free radicals Plasma modification is a versatile surface treatment that is widely employed to add chem ically reactive functional groups to polymer surfaces in order to promote hydrophilicity and achieve low-fouling membrane surfaces [202]. Low-temperature plasma-induced grafting has been reported to increase the permeance and antifouling property of the PVDF and PES/PSF membranes by Z. Zhao et al. [203]. ...