Hou Ip Lao’s scientific contributions

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Publications (1)


Fig. 1. Western Blot analysis on different laccases. A) Western blot analysis on the expression of NSP4-Lac1326, BPUL Laccase, and NSP4-tvel5 laccase B) Relative expression level of laccases under 1 mM IPTG induction. Recombinant proteins containing C-terminal His-tagged residues were expressed in E. coli BL21 (DE3), followed by purification by HEPES solution. Anti-RNA polymerase beta was used as a loading control. Error Bar represented SD of the mean.
Fig. 2. Expression and secretion of laccases under different culturing conditions. A) Western blot analysis of Laccases purified from the lysed cells. B) Western blot analysis of Laccases purified from the cell-free medium.
Fig. 3. Decolorization of indigo carmine by different recombinant laccases at 37 • C. (A) Decolorization of the indigo carmine dye across 4 days as measured with OD (610 nm). The experiment was triplicated with mean and SD was shown in the error bar. (B) Enzyme activity of the three laccases, estimated with indigo carmine decolorization, under 37 • C incubation in a 96-h interval.
Fig. 4. LC-MS analysis of β-estradiol degradation by recombinant laccases. A) Chromatogram of β-estradiol by NSP4-Lac1326 and NSP4-tvel5 Laccase from cell lysates and cell-free medium extraction, with pET-11a as control B) LC-MS analysis of β-estradiol degradation by different laccases. Protein expression from cell lysed and cell free medium (Ni: Nickle Pulldown Assay) was from E. coli induced under the condition of OD 600 = 0.4, IPTG = 0.4 mM and 25 • C.
Plasmids used in this study.
Expression, secretion and functional characterization of three laccases in E. coli
  • Article
  • Full-text available

March 2022

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91 Reads

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10 Citations

Synthetic and Systems Biotechnology

Yating Mo

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Hou Ip Lao

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Sau Wa Au

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[...]

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Leo Chi U. Seak

Endocrine Disrupting Chemicals (EDCs) are a group of molecules that can influence hormonal balance, causing disturbance of the reproductive system and other health problems. Despite the efforts to eliminate EDC in the environment, all current approaches are inefficient and expensive. In previous research, studies revealed that laccase-producing microorganisms may be a potential candidate for EDC degradation, as laccases have been found to be able to degrade many kinds of EDCs effectively and steadily. Here, we created two recombinant laccases, each fused with secretion peptide, Novel Signal Peptide 4 (NSP4), and expressed them in Escherichia coli (E. coli, BL21), together with one laccase without secretion peptide. We first optimized the culture condition of expressing these laccases. Then, we test the activity of the recombinant laccases of decolorizing of a synthetic dye, indigo carmine. Finally, we confirmed the secreted can degrade one of the EDCs, β-estradiol, showing the potential of using the laccase secretion system to degrade toxic compounds.

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Citations (1)


... Recent research has revealed that laccase heterologous expression and secretion may be accomplished using modified E. coli. When expressing extracellular enzymes, however, E. coli easily forms inclusion bodies and preserves part of the produced enzyme in these inclusions, heterologous laccase expression in E. coli offers a possible strategy for PE biodegradation 87,88 . Microalgae as phototrophic microorganisms are so advantageous for use as microbial cell factories because of their high growth rates, scalability, easy and low-cost cultivation, and potentially suitable for genetic manipulation. ...

Reference:

The Role and Application of Microbial Enzymes in Microplastics’ Bioremediation: Available and Future Perspectives
Expression, secretion and functional characterization of three laccases in E. coli

Synthetic and Systems Biotechnology