Hong Wang’s research while affiliated with Ningxia University and other places

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Publications (7)


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Structural characterization and protective effect against myocardial fibrosis of polysaccharide from Stellariae Radix (Stellaria dichotoma L. var. lanceolata Bge.)
  • Preprint
  • File available

April 2024

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8 Reads

Hong Wang

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Pilian Niu

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Haishan Li

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[...]

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Li Peng

Cardiovascular disease is characterized by thedevelopment of myocardial fibrosis, Stellariae Radix is a traditional Chinese medicine. A novel polysaccharide named SRP-1 from Stellariae Radix was structurally identified and its effect against myocardial fibrosis was explored. SRP-1 was extracted from Stellariae Radix and purified by DEAE-52 cellulose chromatography. According to physicochemical methods and monosaccharide composition analysis, SRP-1 was found to be mainly composed of galactose, glucose, xylose, fructose, mannose, and rhamnose, and its weight average molecular weight (Mw) was 31,309 Da. Tests of activity against myocardial fibrosis indicated marked downregulation of the expression of Col I, Col III, α-SMA, and proteins related to the TGF-β/Smad signaling pathway, which were induced by TGF-β1, in mouse cardiac fibroblasts pretreated with SRP-1. In addition, SRP-1 restrained the abnormal growth of cardiac fibroblasts cells and the expression of Snail 1 , Snail 2 , Twist 1 , and Slug mRNA in vitro. SRP-1 can reduce the expression of TGF-β/Smad signaling pathway related proteins induced by TGF-β1. Taken together,SRP-1 can protect CFs from myocardial fibrosis induced by TGF-β1 by inhibiting TGF-β/Smad signaling. These results indicate that Stellariae Radix polysaccharide may warrant further analysis as a novel therapeutic agent for cardiovascular diseases.

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Supercritical CO2 fluid extract from Stellariae Radix ameliorates 2,4-dinitrochlorobenzene-induced atopic dermatitis by inhibit M1 macrophages polarization via AMPK activation

February 2024

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4 Reads

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3 Citations

Environmental Toxicology

Yin chai hu (Radix Stellariae) is a root medicine that is frequently used in Chinese traditional medicine to treat fever and malnutrition. In modern medicine, it has been discovered to have anti-inflammatory, anti-allergic, and anticancer properties. In a previous study, we were able to extract lipids from Stellariae Radix using supercritical CO2 extraction (SRE), and these sterol lipids accounted for up to 88.29% of the extract. However, the impact of SRE on the development of atopic dermatitis (AD) has not yet been investigated. This study investigates the inhibitory effects of SRE on AD development using a 2,4-dinitrochlorobenzene (DNCB)-induced AD mouse model. Treatment with SRE significantly reduced the dermatitis score and histopathological changes compared with the DNCB group. The study found that treatment with SRE resulted in a decrease of pro-inflammatory cytokines TNF-α, CXC-10, IL-12, and IL-1β in skin lesions. Additionally, immunohistochemical analysis revealed that SRE effectively suppressed M1 macrophage infiltration into the AD lesion. Furthermore, the anti-inflammatory effect of SRE was evaluated in LPS + INF-γ induced bone marrow-derived macrophages (BMDMs) M1 polarization, SRE inhibited the production of TNF-α, CXC-10, IL-12, and IL-1β and decreased the expression of NLRP3. Additionally, SRE was found to increase p-AMPKT172 , but had no effect on total AMPK expression, after administration of the AMPK inhibitor Compound C, the inhibitory effect of SRE on M1 macrophages was partially reversed. The results indicate that SRE has an inhibitory effect on AD, making it a potential therapeutic agent for this atopic disorder.


Figure 4. Cont.
α-diversity index of gut microbiota in each group.
Effects of CSRPs on PI values of probiotics.
Evaluation of Prebiotic Activity of Stellariae Radix Polysaccharides and Its Effects on Gut Microbiota

November 2023

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26 Reads

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2 Citations

This study aims to evaluate the prebiotic potential of polysaccharides derived from Stellariae Radix (SRPs) and explore their influence on the gut microbiota composition in mice. Lactobacillus acidophilus and Bifidobacterium longum were cultivated in an MRS medium, while their growth kinetics, clumping behavior, sugar utilization, pH variation, growth density, and probiotic index were meticulously monitored. Additionally, the impact of crude Stellariae Radix polysaccharides (CSRP) on the richness and diversity of gut microbiota in mice was assessed via 16S rDNA sequencing. The results demonstrated the remarkable ability of CSRPs to stimulate the proliferation of Lactobacillus acidophilus and Bifidobacterium longum. Moreover, the oral administration of CSRPs to mice led to a noticeable increase in beneficial bacterial populations and a concurrent decrease in detrimental bacterial populations within the intestinal flora. These findings provided an initial validation of CSRPs as a promising agent in maintaining the equilibrium of gut microbiota in mice, thereby offering a substantial theoretical foundation for developing Stellariae Radix as a prebiotic ingredient in various applications, including food, healthcare products, and animal feed. Furthermore, this study presented novel insights for the exploration and utilization of Stellariae Radix resources.


Integrative metabolome and transcriptome analyses reveal the differences in flavonoid and terpenoid synthesis between Glycyrrhiza uralensis (licorice) leaves and roots

November 2023

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24 Reads

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2 Citations

Food Science and Biotechnology

Licorice from Glycyrrhiza uralensis roots is used in foods and medicines. Although we are aware that licorice roots and leaves have distinct material compositions, the specific reasons for these differences remain unknown. Comparison of the metabolomes and transcriptomes between the leaves and roots revealed flavonoids and triterpenoid saponins were significantly different. Isoflavones were enriched in roots because of upregulation of genes encoding chalcone isomerase and flavone synthase, which are involved in isoflavone synthesis. Six triterpenoid saponins were significantly enriched only in the roots. The leaves did not accumulate glycyrrhetinic acid because of low expression levels of genes involved in its synthesis. A gene encoding a UDP glycosyltransferase, which likely catalyzes the key step in the transformation of glycyrrhetinic acid to glycyrrhizin, was screened. Our results provide information about the differences in flavonoid and triterpenoid synthesis between roots and leaves, and highlight targets for genetic engineering.


Figure 9. Venn diagram analysis of SDMs in different comparison groups. Figure 9. Venn diagram analysis of SDMs in different comparison groups.
The Impact of Growth Years on the Medicinal Material Characteristics and Metabolites of Stellaria dichotoma L. var. lanceolata Bge. Reveals the Optimal Harvest Age

June 2023

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20 Reads

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5 Citations

Plants

The original plant of Chinese medicine Stellariae Radix (Yin Chai Hu) is Stellaria dichotoma L. var. lanceolata Bge (abbreviated as SDL). SDL is a perennial herbaceous plant and a characteristic crop in Ningxia. Growth years are vital factors that affect the quality of perennial medicinal materials. This study aims to investigate the impact of growth years on SDL and screen for the optimal harvest age by comparing the medicinal material characteristics of SDL with different growth years. Additionally, metabolomics analysis using UHPLC-Q-TOF MS was employed to investigate the impact of growth years on the accumulation of metabolites in SDL. The results show that the characteristics of medicinal materials and the drying rate of SDL gradually increase with the increase in growth years. The fastest development period of SDL occurred during the first 3 years, after which the development slowed down. Medicinal materials characteristics of 3-year-old SDL exhibited mature qualities with a high drying rate, methanol extract content, and the highest content of total sterols and total flavonoids. A total of 1586 metabolites were identified, which were classified into 13 major classes with more than 50 sub-classes. Multivariate statistical analysis indicated significant differences in the diversity of metabolites of SDL in different growth years, with greater differences observed in metabolites as the growth years increased. Moreover, different highly expressed metabolites in SDL at different growth years were observed: 1–2 years old was beneficial to the accumulation of more lipids, while 3–5 years old was conducive to accumulating more alkaloids, benzenoids, etc. Furthermore, 12 metabolites accumulating with growth years and 20 metabolites decreasing with growth years were screened, and 17 significantly different metabolites were noted in 3-year-old SDL. In conclusion, growth years not only influenced medicinal material characteristics, drying rate, content of methanol extract, and total sterol and flavonoid contents, but also had a considerable effect on SDL metabolites and metabolic pathways. SDL planted for 3 years presented the optimum harvest time. The screened significantly different metabolites with biological activity, such as rutin, cucurbitacin e, isorhamnetin-3-o-glucoside, etc., can be utilized as potential quality markers of SDL. This research provides references for studying the growth and development of SDL medicinal materials, the accumulation of metabolites, and the selection of optimal harvest time.


Metabolomic Analysis Reveals the Metabolic Diversity of Wild and Cultivated Stellaria Radix (Stellaria dichotoma L. var. lanceolata Bge.)

February 2023

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50 Reads

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8 Citations

Plants

Stellaria Radix, called Yinchaihu in Chinese, is a traditional Chinese medicine, which is obtained from the dried roots of Stellaria dichotoma L. var. lanceolata Bge. Cultivated yinchaihu (YCH) has become a main source of production to alleviate the shortage of wild plant resources, but it is not clear whether the metabolites of YCH change with the mode of production. In this study, the contents of methanol extracts, total sterols and total flavonoids in wild and cultivated YCH are compared. The metabolites were analyzed by ultra-high performance liquid chromatography–tandem time-of-flight mass spectrometry. The content of methanol extracts of the wild and cultivated YCH all exceeded the standard content of the Chinese Pharmacopoeia. However, the contents of total sterols and total flavonoids in the wild YCH were significantly higher than those in the cultivated YCH. In total, 1586 metabolites were identified by mass spectrometry, and 97 were significantly different between the wild and cultivated sources, including β-sitosterol, quercetin derivatives as well as many newly discovered potential active components, such as trigonelline, arctiin and loganic acid. The results confirm that there is a rich diversity of metabolites in the wild and cultivated YCH, and provide a useful theoretical guidance for the evaluation of quality in the production of YCH.


Comparative metabolomics provides novel insights into correlation between dominant habitat factors and constituents of Stellaria Radix (Stellaria dichotoma L. var. lanceolata Bge.)

November 2022

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29 Reads

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10 Citations

Stellaria dichotoma L. var. lanceolata Bge. (SDL) is the original plant of the traditional Chinese medicine Yinchaihu (Stellaria Radix). It is mainly distributed in the arid desert areas of northwest China, which is the genuine medicinal material and characteristic cultivated crop in Ningxia. This study aims to analyze the effects of different origins on SDL metabolites and quality, as well as to screen the dominant habitat factors affecting SDL in different origins. In this study, metabolites of SDL from nine different production areas were analyzed by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF MS) based metabolomics. And field investigations were conducted to record thirteen habitat-related indicators. Results showed that 1586 metabolites were identified in different origins, which were classified as thirteen categories including lipids, organic acids and organic heterocyclic compounds derivatives. Multivariate statistical analysis showed that the metabonomic spectra of SDL from different origins had various characteristics. What’s more, co-expression network correlation analysis revealed that three metabolites modules (MEturquoise, MEbrown and MEblue) were more closely with the habitat factors and 104 hub metabolites were further screened out as the habitat-induced metabolite indicators. Besides, soil texture, soil pH value and soil total salt content were found as the dominant habitat factors which affect SDL metabolites. In conclusion, the study showed different habitat factors had various effects on SDL’s quality and established relationship between them, which provide reference for revealing SDL’s genuineness formation mechanism and guiding industrial crops practical production by habitat factors selection.

Citations (6)


... Stellariae Radix (SR) is the dried root of Stellaria dichotoma L.var. lanceolata Bge, family Caryophyllaceae (Wu et al., 2024). It is efficient in clearing deficiency heat and removing chancre heat and is commonly used clinically for yin deficiency fever, bone vapor, and labor heat (Wu et al., 2024;Li et al., 2017). ...

Reference:

Digital identification and adulteration analysis of Codonopsis Radix and Stellariae Radix based on the “digital identity” of chemical compositions
Supercritical CO2 fluid extract from Stellariae Radix ameliorates 2,4-dinitrochlorobenzene-induced atopic dermatitis by inhibit M1 macrophages polarization via AMPK activation
  • Citing Article
  • February 2024

Environmental Toxicology

... changes in gene expression with the accumulation of stress-related metabolites [46]. Similarly, in Glycyrrhiza uralensis, integrated approaches have been employed to explore the biosynthesis of flavonoids and triterpenoids, highlighting key regulatory genes that contribute to the accumulation of bioactive compounds [47]. These methodologies have also been applied in crops like Zea mays [48], and other crops such as tea [7], watermelon [49], and Solanum nigrum L. [50], where researchers have investigated the interplay between genetic variation and metabolite profiles in response to environmental factors. ...

Integrative metabolome and transcriptome analyses reveal the differences in flavonoid and terpenoid synthesis between Glycyrrhiza uralensis (licorice) leaves and roots
  • Citing Article
  • November 2023

Food Science and Biotechnology

... Interestingly, a number of studies have demonstrated the various health promoting properties of L. sulphureus polysaccharides (LSP) including anti-inflammatory (Lu et al., 2023), anti-cancer (Jen et al., 2024), hepatoprotective, antioxidant activity (Zhao et al., 2019), and hypoglycemic activity (Hwang and Yun, 2010). Many reports have shown that modeling experimental data using kinetic models is one of the effective ways to study the effects of prebiotic compounds on probiotics (Altieri et al., 2016;Bernal-Castro et al., 2019;Montes et al., 2024;Wang et al., 2023). However, there was limited investigation into the impact of LSP on gut microbiota or application of any mathematical model. ...

Evaluation of Prebiotic Activity of Stellariae Radix Polysaccharides and Its Effects on Gut Microbiota

... On the other hand, the duration of planting can alter soil physicochemical properties (such as pH, organic matter, nitrogen, phosphorus, and potassium levels) as well as the microbial environment, which in turn affects the content of active components [46,47]. More critically, autotoxicity may occur, leading to continuous cropping obstacles and other negative effects [48].This study also revealed that fungal diversity in rhizosphere soil of high planting years S. lanceolata was more abundant, and with increasing planting years, the abundance of detrimental bacteria such as Aspergillus, Sarocladium, and Paraphoma increased, while beneficial ones including Arthrobacter, Nocardioides, Mesorhizobium, and Chaetomium decreased. ...

The Impact of Growth Years on the Medicinal Material Characteristics and Metabolites of Stellaria dichotoma L. var. lanceolata Bge. Reveals the Optimal Harvest Age

Plants

... As of now, S. lanceolata has developed into a distinctive superior medicinal plant variety and important crop in Ningxia province, particularly in Tongxin County, which has become the largest planting area nationwide, supplying over 80% of S. lanceolata in the market. However, compared to wild S. lanceolata, the natural habitat of cultivated S. lanceolata has undergone significant changes, resulting in alterations in its characteristics, quality, composition, and efficacy [11]. In recent years, particularly with a fivefold increase in the price of S. lanceolata, its cultivation range has significantly expanded. ...

Metabolomic Analysis Reveals the Metabolic Diversity of Wild and Cultivated Stellaria Radix (Stellaria dichotoma L. var. lanceolata Bge.)

Plants

... lanceolata Bge. (S. lanceolata) is a psammophytic plant species that thrives in the arid and semi-arid regions of Northwest China [1]. Due to its characteristics of being drought-tolerant, resilient to poor soil conditions, and possessing strong vitality, it plays a crucial role in maintaining the ecological balance of the desert steppe [2]. ...

Comparative metabolomics provides novel insights into correlation between dominant habitat factors and constituents of Stellaria Radix (Stellaria dichotoma L. var. lanceolata Bge.)