Ho-Sung Yoon's research while affiliated with Kyungpook National University and other places
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Publications (116)
The prokaryotic microalga Limnothrix redekei KNUA012 isolated from a freshwater bloom sample from Lake Hapcheon, Hapcheon-gun, South Korea, was investigated for its potential as a biofuel feedstock. Microalgae produce straight-chain alkanes/alkenes from acyl carrier protein-linked fatty acyls via aldehyde decarbonylase (AD; EC 1.2.1.3), which can c...
Various abiotic stress factors, such as high temperatures and salinity, have a significant impact on the development and growth of crop plants and ultimately impact crop yield. Previous studies have reported that overexpression of heat-shock-protein (HSP) genes in transgenic plants can enhance stress tolerance under controlled conditions in laborat...
Microalgae are versatile, profitable, and promising sources of bioenergy and high-value products, having various applications in the biotechnology industry. Herein, G. emersonii KNUA204 was isolated from Ulleungdo Island, South Korea, and exposed to stressors, i.e., MgCl2 (75 and 150 mM) and NaCl (200 and 400 mM), to investigate improvement in its...
Pharmaceutical and personal care products (PPCPs) are discharged into receiving water bodies mainly from sewage treatment plants. Due to the inefficient removal in conventional wastewater treatment facilities, PPCPs have become a major concern to aquatic ecosystems, water quality, and public health worldwide since they cause harmful effects on aqua...
Microbes are essential in biofloc technology for controlling nitrogen levels in water. The composition and function of microorganisms with biofloc systems were reported; however, data on microorganisms other than bacteria, such as algae (which are essential in the nitrogen cycle) and zooplankton (which are bacterial and algal predators), remain lim...
Abiotic stress induces reactive oxygen species (ROS) generation in plants, and high ROS levels can cause partial or severe oxidative damage to cellular components that regulate the redox status. Here, we developed salt-tolerant transgenic rice plants that overexpressed the dehydroascorbate reductase gene (OsDHAR1) under the control of a stress-indu...
In the past, biomass production using microalgae culture was dependent on inorganic carbon sources as microalgae are photosynthetic organisms. However, microalgae utilize both organic and inorganic carbon sources, such as glucose. Glucose is an excellent source of organic carbon that enhances biomass yield and the content of useful substances in mi...
In this study, we characterized the potential of colony-forming green algae, Botryosphaerella sudetica KNUA107, isolated from Ulleung Island, South Korea, as a bioresource and analyzed the effects of mixotrophic cultivation on its bioresource production efficiency. Internal transcribed spacer (ITS) (ITS1, 5.8S, and ITS2), ribulose bisphosphate carb...
The diversity indices of eukaryotic microalgal groups in the Jeonglyeongchi, Waegok, and Wangdeungjae marshes of Mount Jiri, Korea, were measured using Illumina MiSeq and culture-based analyses. Waegok marsh had the highest species richness, with a Chao1 value of 828.00, and the highest levels of species diversity, with Shannon and Simpson index va...
The reverse osmosis system is one of the most advanced membrane-based water treatment technologies that can convert a variety of wastewater into drinking and industrial water. However, it has major drawbacks, including the production of a large amount of reject stream that contains concentrated nutrients. Microalgae are well-known for their rapid g...
Key message
Enhanced glutathione content improves lateral root development by positively regulating the transcripts of root development genes responsive to glutathione treatment, thereby increasing the overall productivity of rice plants.
Abstract
Glutathione is primarily known as a cellular antioxidant molecule, but its role in lateral root devel...
There is growing interest in the production of microalgae-based high-value by-products as an emerging green biotechnology. However, a cultivation platform for Oocystis sp. has yet to be established. To overcome this limitation, we examined the effect of bacterial culture additions on the growth and production of valuable compounds the Oocystis sp....
A cold-tolerant unicellular green alga was isolated from a meltwater stream on King George Island, Antarctica. Morphological, molecular, and biochemical analyses revealed that the isolate belonged to the species Chlorella vulgaris. We tentatively named this algal strain C. vulgaris KNUA007 and investigated its growth and lipid composition. We found...
Metagenome studies have provided us with insights into the complex interactions of microorganisms with their environments and hosts. Few studies have focused on microalgae-associated metagenomes, and no study has addressed aquatic microalgae and their bacterial communities in open pond raceways (OPRs). This study explored the possibility of using m...
The microalgal strain Scenedesmus rubescens KNUA042 was identified in freshwater in Korea and characterized by evaluating its stress responses in an effort to increase lipid and carotenoid production. Under a two-stage cultivation process, the algal strain that generally exhibits optimal growth at a nitrate (source of nitrogen) concentration of 0.2...
This study aims to characterize the potential of three strains of microalgal species (Chlorella sorokiniana KNUA114 and KNUA122; C. vulgaris KNUA104) for use as feedstock, based on their fatty acid compositions. Each strain was molecularly identified using four marker genes (ITS, SSU, rbcL, and tufA) and phylogenetically characterized. C. sorokinia...
Oryza sativa vacuolar H⁺-pyrophosphatase 1 (OVP1) regulates plant development and osmotic stress response through its function as an electrogenic proton (H⁺) pump in the vacuolar membrane. Here, we show that OVP1-overexpressing transgenic (OTG) rice plants display greater resistance to salt stress and root growth than wild-type (WT) rice plants. Th...
Cerastium arcticum is one of the few flowering plants that thrives in the Arctic, suggesting that it possesses mechanisms for overcoming this extreme environment. To verify the functionality of C. arcticum dehydrin (CaDHN), known to play a protective role during cellular dehydration, the gene was introduced to Arabidopsis and Escherichia coli. Tran...
An excess of reactive oxygen species (ROS) can cause severe oxidative damage to cellular components in photosynthetic cells. Antioxidant systems, such as the glutathione (GSH) pools, regulate redox status in cells to guard against such damage. Dehydroascorbate reductase (DHAR, EC 1.8.5.1) catalyzes the glutathione-dependent reduction of oxidized as...
Abscisic acid-, stress-, and ripening-induced (ASR) genes are involved in responding to abiotic stresses, but their precise roles in enhancing grain yield under stress conditions remain to be determined. We cloned a rice (Oryza sativa) ASR gene, OsASR1, and characterized its function in rice plants. OsASR1 expression was induced by abscisic acid (A...
The chloroplast genome of Micractinium pusillum CCAP 231/1 was completely sequenced. This chloroplast genome has 115,638 bp in length and consists of 111 genes including 81 protein-coding, 4 rRNA, and 26 tRNA genes. The overall GC content of the genome is 35.3%.
The mitochondrial genome of Micractinium pusillum CCAP 231/1 was completely sequenced. This mitogenome has 70,061 bp in length and consists of 62 genes including 32 protein-coding, 3 rRNA, and 27 tRNA genes. The overall GC content of the genome is 31.3%.
The chloroplast genome of Micractinium singularis MM0003 was completely sequenced. This plastome has 139,597 bp in length and consists of 106 genes including 77 protein-coding, 3 rRNA, and 26 tRNA genes. The overall GC content of the genome is 34.0%.
The mitochondrial genome of Micractinium singularis MM0003 was completely sequenced. This mitogenome has 75,931 bp in length and consists of 62 genes including 32 protein-coding, 3 rRNA, and 27 tRNA genes. The overall GC content of the genome is 27.5%.
Ulleungdo and Dokdo are volcanic islands with an oceanic climate located off the eastern coast of South Korea. In the present study, we used barcoded Illumina MiSeq to analyze eukaryotic microalgal genera collected from Seonginbong, the highest peak on Ulleungdo, and from groundwater sites on Dongdo and Seodo Islands, which are part of Dokdo. Speci...
Water scarcity and emerging demands for renewable energy have increased concerns about energy security and advanced wastewater treatment, and microalgae have emerged as promising candidates to solve these problems. This study assesses the feasibility of microalgal wastewater treatment, and the utilization of the resulting microalgal biomass, as a r...
Ascorbic acid (AsA) is an abundant component of plants and acts as a strong and active antioxidant. In order to maintain the antioxidative capacity of AsA, the rapid regeneration of AsA is regulated by dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR). To understand how MDHAR functions under extreme temperature conditions...
Green algae produce fatty acid components that can be used in biofuel production without the need for additional nutrients. We aimed to elucidate the contribution of the T. obliquus KNUA019 through the control of the optimal culture (solvent, culture time, nitrogen, and phosphorus) conditions and thermal analysis (DTA and TGA curves) that affects f...
Ototoxicity, or adverse pharmacological effects on the inner ear or auditory nerve, is a common side effect of cisplatin, a platinum-based drug widely used in anticancer chemotherapy. Although the incidence of ototoxicity is high among patients that receive cisplatin therapy, there is currently no effective treatment for it. The generation of exces...
Cyanobacterial 2-Cys peroxiredoxin (thioredoxin peroxidase, TPX) comprises a family of thiol antioxidant enzymes critically involved in cell survival under oxidative stress. In our previous study, a putative TPX was identified using a proteomics analysis of rice (Oryza sativa L. japonica, OsTPX) seedlings exposed to oxidative stress. This OsTPX gen...
Objectives:
To improve the oxidative stress tolerance, biomass yield, and ascorbate/dehydroascorbate (AsA/DHA) ratio of Synechococcus elongatus PCC 7942 in the presence of H2O2, by heterologous expression of the dehydroascorbate reductase (DHAR) gene from Brassica juncea (BrDHAR).
Results:
Under H2O2 stress, overexpression of BrDHAR in the trans...
Monodehydroascorbate reductase (MDHAR), which is responsible for growth, development and stress response in plants, is a key enzyme in the maintenance of the ascorbate (AsA) pool through the AsA–glutathione (AsA–GSH) cycle and is induced by abiotic stresses. It has highly conserved regions containing FAD- and NAD(P)H-binding domains. In particular,...
[This corrects the article DOI: 10.1371/journal.pone.0158841.].
Ascorbic acid (AsA) maintains redox homeostasis by scavenging reactive oxygen species from prokaryotes to eukaryotes, especially plants. The enzyme monodehydroascorbate reductase (MDHAR) regenerates AsA by catalysing the reduction of monodehydroascorbate, using NADH or NADPH as an electron donor. The detailed recycling mechanism of MDHAR remains un...
A unicellular green alga, Chlorella vulgaris KNUA027, was isolated from the roots of Panax ginseng seedlings and its biotechnological potential was investigated. The results of GC/MS analysis showed that C. vul-garis KNUA027 was rich in nutritionally important polyunsaturated fatty acids (PUFAs) such as alpha-linolenic acid (C18:3 ω3, 45.8%, 50.8 m...
Exogenous effect of ascorbate and its analogue on the stress sensitivity of ara2Δ yeast cells.
(DOCX)
Cellular response and redox state in por1Δ yeast cells under oxidative stress.
(DOCX)
Stress sensitivity and redox state of por1△ yeast cells under oxidative stress.
(DOCX)
Cellular response and redox state in yeast under oxidative stress.
(DOCX)
Response of OsMDHAR-expressing yeast cells to abiotic stressors.
(DOCX)
Monodehydroascorbate reductase (MDHAR; EC 1.6.5.4) is an important enzyme for ascorbate recycling. To examine whether heterologous expression of MDHAR from Oryza sativa (OsMDHAR) can prevent the deleterious effects of unfavorable growth conditions, we constructed a transgenic yeast strain harboring a recombinant plasmid carrying OsMDHAR (p426GPD::O...
Strains and plasmids used in this study.
(DOCX)
Cellular response and redox state in ara2△ yeast cells under oxidative stress and exogenous effect of AsA and its analogue.
(DOCX)
Oligonucleotide sequence used in this study.
(DOCX)
The cyanobacterial aldehyde deformylating oxygenase (cADO) is a key enzyme that catalyzes the unusual deformylation of aliphatic aldehydes for alkane biosynthesis and can be applied to the production of biofuel in vitro and in vivo. In this study, we determined crystal structures of two ADOs from Limnothrix sp. KNUA012 (LiADO) and Oscillatoria sp....
A filamentous cyanobacterium, Limnothrix sp. KNUA012, was axenically isolated from a freshwater bloom sample in Lake Hapcheon, Hapcheon-gun, Gyeongsangnam-do, Korea. Its morphological and molecular characteristics led to identification of the isolate as a member of the genus Limnothrix. Maximal growth was attained when the culture was incubated at...
Dehydroascorbate reductase (DHAR) is a key enzyme involved in the recycling of ascorbate, which catalyses the glutathione (GSH)-dependent reduction of oxidized ascorbate (dehydroascorbate, DHA). As a result, DHAR regenerates a pool of reduced ascorbate and detoxifies reactive oxygen species (ROS). In previous experiments involving transgenic rice,...
Cryotolerant eukaryotic microalgae were isolated from meltwater streams on Ardley Island and King George Island in Antarctica, and their morphological, molecular, and physiological characteristics were investigated. Owing to their simple morphology, distinctive characters were not observed with neither light microscopy nor transmission electron mic...
In the present study, a novel technique, which involves numerical computation of the mixing length of algae particles in raceway ponds, was used to evaluate the mixing process. A value of mixing length that is higher than the maximum streamwise distance of algae cells indicates that the cells experienced an adequate turbulent mixing in the pond. A...
The thermal properties affected by particle size of microbial cell were investigated by the simultaneous thermogravimetry (TG) and differential thermal analysis (DTA). The particle size, which could affect the heat evolved, occurred from the combustion of the microbial cells. The model microorganism used in this study is Nostoc, a genus of flamento...
Monodehydroascorbate reductase (MDHAR; EC 1.6.5.4) is a key enzyme in the reactive oxygen species (ROS) detoxification system of plants. The participation of MDHAR in ascorbate (AsA) recycling in the ascorbate–glutathione cycle is important in the acquired tolerance of crop plants to abiotic environmental stresses. Thus, MDHAR represents a strategi...
Background
In contrast with wild species, cultivated crop genomes consist of reshuffled recombination blocks, which occurred by crossing and selection processes. Accordingly, recombination block-based genomics analysis can be an effective approach for the screening of target loci for agricultural traits.
Results
We propose the variation block meth...
This review covers recent research and development (R&D) activities in the field of algae-based biofuels in Korea. As South Korea's energy policy paradigm has focused on the development of green energies, the government has funded several algae biofuel R&D consortia and pilot projects. Three major programs have been launched since 2009, and signifi...
The purpose of this study is to investigate the frequency of the subclinical synovitis in hand or wrist joints of the SLE patients using ultrasonography (US) and to correlate them with clinical parameters. Forty-eight systemic lupus erythematosus (SLE) patients without musculoskeletal (MS) involvement were enrolled and underwent clinical and labora...
Plants are exposed to various environmental stresses and have therefore developed antioxidant enzymes and molecules to protect their cellular components against toxicity derived from reactive oxygen species (ROS). Ascorbate is a very important antioxidant molecule in plants, and monodehydroascorbate reductase (MDHAR; EC 1.6.5.4) and dehydroascorbat...
STN8 kinase is involved in photosystem II (PSII) core protein phosphorylation (PCPP). To examine the role of PCPP in PSII repair during high light (HL) illumination, we characterized a T-DNA insertional knock-out mutant of the rice (Oryza sativa) STN8 gene, osstn8. In this mutant, PCPP was significantly suppressed and the grana were thin and elonga...
STN8 kinase is involved in photosystem II (PSII) core protein phosphorylation (PCPP). To examine the role of PCPP in PSII repair during high light (HL) illumination, we characterized a T-DNA insertional knock-out mutant of the rice (Oryza sativa) STN8 gene, osstn8. In this mutant, PCPP was significantly suppressed and the grana were thin and elonga...
Ascorbate (vitamin C) plays an important role in detoxification of reactive oxygen species (ROS) in most living organisms. Monodehydroascorbate reductase (MDHAR; EC 1.6.5.4) is crucial to regeneration of the oxidized form of ascorbate (monodehydroascorbate) so that it can be recycled to maintain ROS scavenging ability. The MDHAR gene from Brassica...
A previously uncultured cyanobacterium, strain KNUA009, was axenically isolated from a meltwater stream on Barton Peninsula, King George Island, South Shetland Islands, Antarctica. Molecular evidences showed that the isolate belongs to groups of globally distributed cryosphere cyanobacterial clones and this new isolate represents the first laborato...
Objective:
Dyslipidemia, a risk factor for cardiovascular diseases, is more prevalent in patients with rheumatoid arthritis (RA) than in the general population. We investigated whether single-nucleotide polymorphisms (SNP) modulating low-density lipoprotein (LDL) cholesterol affect susceptibility, severity, and progression of RA.
Methods:
We enr...
Glutathione (GSH), a low-molecular-weight tripeptide molecule that plays an important role in cell function and metabolism as an antioxidant, is synthesized by γ-glutamylcysteine synthetase a