Herve Serieys's research while affiliated with French National Institute for Agriculture, Food, and Environment (INRAE) and other places

Publications (45)

Article
He amplified fragments with RAPD primers in 15 accessions belonging to 12 species pf Helianthus genus. Thirty four primers out of eighty primers assayed lead to fragments present in one or two sections hut not in 3 sections. These fragments enable us to construct dendrograms based upon either Nei or Jaccard distances and to propose a phylogram acco...
Article
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RÉSUMÉ Dans les régions arides ou semi-arides, l’eau est le facteur limitatif le plus important de la production agricole. La germination est une phase sensible au manque d’eau. Des expériences ont été faites dans le but d’examiner l’influence du stress dû au manque d’eau sur la germination des semences de trois génotypes de tournesol (Oro 9, Miras...
Article
Common sunflower (i.e., Helianthus annuus) is occasionally crossed with other Helianthus species to gain favourable agronomic traits. An interspecific cross between H. annuus and Helianthus mollis was used to examine events that occurred during the five first generations. First generation hybrid plants were verified using GISH, and F1 hybrid (#17)...
Article
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The genetic base of sunflower elite lines is very narrow, due to many years of selection and breeding. To broaden the genetic diversity of the cultivated sunflower, in 1995 73 wild sunflower populations were crossed with 3 cultivated lines (Testers), and 219 hybrid offspring’s were evaluated in the field. GCA and SCA effects were computed suggestin...
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INRA hosted the national Jerusalem artichoke collection in Rennes and Clermont-Ferrand until 2005. It now takes place in Montpellier. The list of cultivar-clones and of wild accessions is provided as well as main data on expected yield, earliness, female fertility, and disease susceptibility to viruses and Oidium. Descriptions of some traits (antho...
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Sunflower (2n=17) belongs to the Helianthus genus (Asteraceae). Wild Helianthus species display morphological variation for branching and stem number, for architecture and seed size, and for resistance to abiotic and biotic stresses due to which they thrive in different environments in North America. The genus is divided into botanical sections, tw...
Article
Seventy-seven wild sunflower accessions from USA were compared in France (Mauguio) for 13 quantitative characters using multivariate methods, including clustering, principal component and canonical discriminate analyzes to assess the patterns of morphological and climatic variation. Geographic and climatic data from their sampled sites such as lati...
Conference Paper
Full-text available
New sources of major gene resistance to sunflower downy mildew were compared with known resistance genes. All genes appear to come from crosses with wild Helianthus, and most frequently from wild H.annuus. The gene Pl6 has been found in many different ecotypes but resistances which segregate independently from this gene have also been obtained. Gen...
Conference Paper
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Des observations d'attaques naturelles, sous irrigation, de Mildiou dû à Plasmopara halstedii, sur une importante série de génotypes de Helianthus annuus cultivés, ont permis de mettre en évidence une très large gamme de comportement, même en absence de gène de résistance spécifique Pl efficace. Ce comportement est indépendant du pathotype présent...
Article
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The effect of osmotic and water stresses on various morphological traits was studied in eight experimental varieties of sunflower bred for Moroccan conditions or issued from interspecific hybridization with H. argophyllus or H. debilis. Seed yield of the same genotypes was also evaluated under rainfed and irrigated field conditions. Shoot and root...
Article
Full-text available
The effect of osmotic and water stresses on various morphological traits was studied in eight experimental varieties of sunflower bred for Moroccan conditions or issued from interspecific hybridization with H.argophyllus or H.debilis. Seed yield of the same genotypes was also evaluated under rainfed and irrigated field conditions. Shoot and root gr...
Article
The possibility of gene flow between cultivated and wild species of different crops was assessed, focussing on sunflower and its wild relatives or cultivated forms (annual Helianthus annuus, H. argophyllus, H. bolanderi, H. petiolaris, and perennial H. rigidus, H. tuberosus). Data were compiled from the literature and an attempt was made at determi...
Article
HA89, a sunflower line susceptible to Phomopsis, was crossed with a resistant line, LR4-17. Two hundred and forty-one F2/F3 progenies and 232 recombinant inbred (RI) lines were derived from this cross. F2/F3 progenies were tested in semi-natural infections in 1994. F7, F8 and F9 were tested with semi-natural infections in 1997, 1998 and 1999, respe...
Article
Hybridisation between the annual diploid sunflower ( Helianthus annuus)and the perennial diploid species Helianthus mollis and Helianthus orgyalis was obtained by means of a normal crossing procedure or embryo rescue. Hybridisation success was low. All plants examined cytologically appeared to be diploid. However, the phenotypes of these diploids w...
Article
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To obtain introgressed sunflower lines with improved disease resistance, interspecific crosses were performed with foreign perennial species. We report on several unusual features displayed by these hybrid plants. The methods used to produce the kernels affected yield and genotypes of progeny. Phenotypic traits and DNA markers were investigated in...
Article
. Eighty-four plants obtained from the pollination of the annual diploid cultivated sunflower, Helianthus annuus, by the perennial diploid species H. mollis were obtained naturally from mature seeds (62 plants) or following embryo rescue procedures (22 plants) and subsequently studied for phenotype traits and DNA markers. Twenty-two plants were obt...
Article
Orobanche cumana (broomrape) poses a serious threat to sunflower cultivation in many countries since there is no effective method of controlling the pathogen. It is known that wild species of the genus Helianthus show resistance to different pathogens. Our goal was to identify resistant wild species and derived interspecific hybrids &lpar...
Article
Fourteen recombinant inbred lines of sunflower (Helianthus annuus L.) and their parents (PAC-2 and RHA-266) were tested for their organogenesis ability. Seeds were surface sterilized and germinated on hormone free half strength MS basal medium containing 10 g l-1 sucrose solidified with five different gelling agents: Phytagar (Gibco laboratoires) 3...
Article
Protoplasts of 6 alloplasmic and 2 euplasmic sunflower inbred lines were isolated from dark grown seedling hypocotyls with a density of 2×104 protoplasts/ml. The protoplast suspension was mixed with a solution of 0.5% agarose (sigma – type 1), then pipetted in droplets of about 1000 protoplasts. Droplets were surrounded by two different liquid medi...
Article
 Forty taxa belonging to 36 species and four unclassified accessions of Helianthus were studied using RAPD technology. Single ten-mer primers were screened for those amplifying fragments common to several species. We found that when several species shared a common fragment, they belong to the same section of the genus. Moreover, we also found that...
Article
Molecular variation at atpA gene loci of mitochondrial genome was analised and characterized in 21 CMS lines of sunflower. Southern blot was applied to genomic DNA and mtDNA using a fragment containing partial atpA gene and orf H522 as a probe (4.1kb). After Southern analysis and mtDNA restriction pattern comparison, four kinds of DNA sequences wer...
Article
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A method based upon targetting of introgressed markers in a Phomopsis-resistant line (R) of cultivated sunflower, issuing from a H. argophyllus cross was used to mark the Phomopsis resistance regions. Our study was based upon 203 F(3) families derived from a cross between an inbred line susceptible to Phomopsis (S1) and the introgressed resistant l...
Article
Crosses were made between three cytoplasmic male sterile and six restorer sunflower inbred lines. F1 hybrids and their parents were studied for their organogenesis ability in a randomized blocks design with three replications. Seeds were surface sterilized, and subsequently germinated on hormone free half strength MS medium. Regeneration medium con...
Article
Restoration of cytoplasmic male sterility (CMS) in sunflower was demonstrated to be controlled by polygenes by analysing 982 effective crosses among 109 self-crossed lines and 16 CMS lines. Two self-crossed lines and one CMS line with distinct genotypes were applied to creation of segregating populations for DNA bulks of the target gene Rf1. Bulked...
Article
Segregation of 48 genetic markers, including one CMS restorer gene, one morphological character gene, six isozymes and 40 RAPD loci, was scored in a backcross progeny of an interspecific hybrid H. argophyllusxH. annuus cv RHA274. A linkage map was generated taking into account segregation distortions for 11 of the 48 loci in the frame of two differ...
Article
Full-text available
Fifteen sunflower (Helianthus annuus L.) cytoplasmic male-sterile, and a single male-fertile, cytotypes were studied by both mtDNA (mitochondrial DNA) restriction fragment length polymorphism (RFLP) and genetical analysis of male-fertility restoration patterns. It was found by multivariate analysis that the two methods of identification of cytoplas...
Conference Paper
Full-text available
We were able, with the study of a wild sunflower species (Helianthus resinosus Small) resistant to Sclerotinia sclerotiorum (Lib.) de Bary, associated with analysis of cultivated sunflower (H. annuus L.) not resistant towards this pathogen, to bring out the specific features of H. resinosus both from the anatomical point of vue and the chemical one...
Article
Full-text available
The genetics of male fertility restoration and the RFLP of mitochondrial DNA were studied for 16 sunflower cytoplasms (15 male-sterile and a male-fertile). Male fertility restoration/male sterility maintenance patterns distinguished 12 cytotypes. Four cytoplasms were completely unrestored so they were not distinguished genetically. The sunflower l...

Citations

... Crop ferality can play an important role in gene flow between crops and their wild relatives and introgression from artificially selected sources can have significant evolutionary consequences (Ashiq Rabbani et al., 1998;Berville et al., 2005;Snow and Campbell, 2005;Devaux et al., 2007;Gering et al., 2019). Furthermore, with the growing adoption of genetically modified (GM) crops worldwide, transgenic flow turned into a widely discussed topic and ferality an issue of concern amongst biosafety specialists. ...
... The most effective and simplest molecular methods for assessing genetic polymorphism are PCR-based techniques. Among such techniques, the Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeats (SSR) markers are widely used, allowing rapid detection of the variability of a large number of genome loci (Sivolap and Solodenko, 1998;Sossey-Alaoui et al., 1998;1999;Markin et al., 2016;Suresha et al., 2017;Yang et al., 2018;Uma et al., 2018). The spectra of DNA fragments, obtained as a result of their amplification, can be used as genetic markers for species identification and barcoding, as well as for determining taxonomic differences between species. ...
... Interestingly, although in most plant species ferulic acid is the major bluegreen fluorophore (Morales et al., 1996), in the case of sunflower there is no ferulic acid bound to cell walls. Small amounts of caffeic acid and trichome secretions (e.g., sesquiterpenes lactones and coumarins) have been described as blue-green fluorophores in sunflower (Spring and Schilling, 1989;Olson and Roseland, 1991;Morales et al., 1996;Tourvieille de Labrouhe et al., 1997;Lichtenthaler and Schweiger, 1998;Rowe et al., 2012). These compounds could be responsible for BGF emission in our experiments. ...
... Obviously, such lines cannot be involved in commercial hybrids due to their heterozygosity and some instability since they lead to nonhomogeneous progenies. Such materials are of interest for breeding purposes when they display any useful trait for breeding, which is probably located in the introgression (Serieys, 1997;Pinochet et al., 2001Serieys, 2009. They are also of interest to map traits and to evaluate the introgression fragment content for useful and flawed traits to ensure that there is no linkage between the useful and flawed traits. ...
... This might be related to changes occurring in tissues in response to the pathogen, such as cell collapse and changes in cell wall composition. Cosson et al. (1993) and Pratts-Pérez et al. (2000) studied wild and cultivated genotypes of sunflower and identified that necrotized tissues synthesize compounds which appear to be phytoalexins. and water-soaking in advancing margins (Hancock, 1972;Lumsden and Dow, 1973). ...
... Breeding for resistance to DM usually concentrates on the search for and incorporation of major genes designated Pl into elite sunflower lines. Simple inheritance of DM genes was discovered rather early (Vranceanu and Stoenescu, 1970;Zimmer, 1974), with different race-specific single dominant resistance genes used worldwide by sunflower breeders (Vear et al., 2008a). This causes the DM pathogen to evolve new races (Ahmed et al., 2012). ...
... Partial resistance to Downy mildew has been studied in France since 2003, in a collaboration programme between INRA, CETIOM and breeders (Oléosem). First results were presented at Fargo (Tourvieille et al., 2004). This paper presents the research carried out in the last 3 years, particularly concerning large-scale trials, and discusses programmes in progress and possible use of this type of resistance in breeding. ...
... Notably, it was previously demonstrated that Ol-associated markers are located on chromosome 14 (Pérez-Vich et al. 2002;Schuppert et al. 2006;Lacombe et al. 2009;Premnath et al. 2016). Previously translocations were discussed for Heliathus genus in terms of divergence between the species, namely H. annuus and H. argophyllus were hypothesized to differ by two reciprocal translocations (Chandler et al. 1986;Quillet et al. 1995), with ve non-reciprocal translocations between the chromosomes of H. annuus and H. argophyllus having been additionally reported (Heesacker et al. 2009). Eight translocations were discussed among the differences between H. annuus and H. petiolaris (Burke et al. 2004). ...
... Similarly, Widuri et al. [52] observed this reduction effect in Phaseolus lunatus only after a prolonged water deficit period. In this sense, other authors demonstrated that root length was positively correlated with drought resistance [52][53][54]. Khalil et al. [55] proved the role of soil texture on tolerance to water stress related to the decrease in fresh weight in roots and shoots. Similar results have been reported on other growth parameters, such as the reduction in vegetative growth or the number of leaves in Phaseolus vulgaris [56,57]. ...
... Bohorova and Atanassov [9] showed that anther cultures of hybrid sunflower are more efficient in forming callus tissue in terms of both the intensity and the rate of the process compared with sunflower lines and cultivars. The dependence of morphogenetic responses on the specific genetic features of individual sunflower genotypes has been described in several research articles [62][63][64][65]. When studying the genetic control of organogenesis in sunflower, Sarrafi et al. [62] observed that both the general and specific combining ability were significant for shoot regeneration and rhizogenesis. ...