Heedoo Lee's research while affiliated with Changwon National University and other places

Publications (35)

Chapter
Extracellular vesicles (EVs) are biological carriers, and EV-associated miRNAs (EV-miRNAs) are considered as a novel biomarker in multiple diseases. Currently, the column-based purification method is used to purify miRNAs from EVs. However, this method of purification is complex, time-consuming, and expensive. Therefore, a simple and cost-effective...
Article
Ozone is a ubiquitous air pollutant that causes lung damage and altered functioning. Evidence suggests that proinflammatory macrophages contribute to ozone toxicity. Herein, we analyzed the role of extracellular vesicles (EVs) and miRNA cargo in ozone-induced macrophage activation. Exposure of mice to ozone (0.8 ppm, 3 h) resulted in increases in B...
Chapter
Extracellular vesicles (EVs) are membranous nanoparticles secreted by nearly all cell types and play a critical role in cell-to-cell crosstalk. EVs can be categorized based on their size, surface markers, or the cell type from which they originate. EVs carry “cargo,” including but not limited to, RNA, DNA, proteins, and small signaling molecules. T...
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Extracellular vesicles are cell-derived membranous vesicles that are secreted into biofluids. Emerging evidence suggests that EVs play an essential role in the pathogenesis of many diseases by transferring proteins, genetic material, and small signaling molecules between cells. Among these molecules, microRNAs (miRNAs), a type of small noncoding RN...
Article
Extracellular vesicles (EVs) in bodily fluids play an essential role in cell-cell crosstalk and potentially serve as novel biomarkers in "liquid biopsy". It is crucial to have a consistent, efficient, and reliable method to separate EVs from bodily fluids. Currently, there is no universally accepted, "best" method to separate EVs. Besides different...
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Acute respiratory distress syndrome (ARDS) is a devastating syndrome responsible for significant morbidity and mortality. Diffuse alveolar epithelial cell death, including but not limited to apoptosis and necroptosis, is one of the hallmarks of ARDS. Currently, no detectable markers can reflect this feature of ARDS. Hyperoxia-induced lung injury is...
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Extracellular vesicles (EVs) present numerous biomedical ways of studying disease and pathology. They function as protective packaging for the delivery of controlled concentrations of miRNAs and effector molecules, including cytokines, chemokines, genetic material, and small signaling molecules. Previous studies of EVs have yielded valuable insight...
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Extracellular vesicles (EV) are secretory membranous elements used by cells to transport proteins, lipids, mRNAs, and microRNAs (miRNAs). While their existence has been known for many years, only recently has research begun to identify their function in intercellular communication and gene regulation. Importantly, cells have the ability to selectiv...
Article
Emerging evidence suggests that extracellular vesicle (EV) -associated microRNAs (miRNAs) are potential diagnostic tools for human diseases. However, the experimental procedure for detection of EV-associated miRNAs (EV-miRNAs) from body fluids is relatively complex and not cost-effective. Due to limited amount of EVs /EV-RNAs, column-based RNA puri...
Chapter
Extracellular vesicles (EVs) are naturally generated nanovesicles which potentially mediate the intercellular communication and interorgan crosstalk. EVs have recently gained significant interest as a promising material for delivery of therapeutics. Small RNAs, including small interfering RNA (siRNA) and microRNA (miRNA), provide a great therapeuti...
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Full-text available
Extracellular vesicles (EVs) are cell-derived membranous vesicles secreted by cells into the extracellular space, which play a role in cell to cell communication. EVs are categorized into 3 groups depending on their size, surface marker, and method of release from the host cell. Recently, EVs have become of interest in the study of multiple disease...
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Emerging evidence suggests that extracellular vesicle (EV)–containing miRNAs mediate intercellular communications in response to noxious stimuli. It remains unclear how a cell selectively sorts the cellular miRNAs into EVs. We report that caveolin-1 (cav-1) is essential for sorting of selected miRNAs into microvesicles (MVs), a main type of EVs gen...
Article
Background Uncontrolled lung inflammation is one of the prominent features in the pathogenesis of lung infection- associated acute lung injury (ALI). Microvesicles (MVs) are extracellular nanovesicles that are generated via direct membrane budding. Methods Bronchoalveolar lavage fluid (BALF) samples were collected from mice with or without intratr...
Article
Despite emerging interest in the role of extracellular vesicle (EV)-containing microRNAs (EV-miRNAs), the existence of functional EV-miRNAs under patho-physiological conditions has been viewed with skepticism. Due to the heterogenicity of EVs, several barriers related to EV-miRNA research are to be explored before the in vivo function of EV-miRNAs...
Article
Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a highly complex process that can be triggered by both noninfectious (sterile) and infectious stimuli. Inflammatory lung responses are one of the key features in the pathogenesis of this devastating syndrome. How ALI/ARDS-associated inflammation develops remains incompletely unde...
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Full-text available
Exosomes (EXOs) are a type of extracellular nanovesicles released from living cells. Accumulating evidence suggests that EXOs are involved in the pathogenesis of human diseases, including lung conditions. In recent years, the potential of EXO-mediated drug delivery has gained increasing interest. In this report, we investigated whether inhaled EXOs...
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Inflammatory lung responses are one of the characterized features in the pathogenesis of many lung diseases, including acute respiratory distress syndrome (ARDS) and chronic obstructive pulmonary disease (COPD). Alveolar macrophages (AMs) and alveolar epithelial cells are the first line of host defense and innate immunity. Due to their central role...
Article
Robust lung inflammation is one of the prominent features in the pathogenesis of acute lung injury (ALI). Macrophage migration and recruitment are often seen at the early stage of lung inflammatory responses to noxious stimuli. Using an acid inhalation-induced lung injury model, we explored the mechanisms by which acid exposure initiates macrophage...
Article
The function of most human long noncoding RNAs (lncRNAs) remains unclear. Our studies identified a highly up-regulated mammalian lncRNA, FOXD3-AS1, known as linc1623 in mouse, in the setting of hyperoxia/reactive oxygen species (ROS)-induced lung injury. We found that ROS induced a robust expression of FOXD3-AS1 in mouse lung tissue. Functionally,...
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Bacterial pneumonia is a common and serious clinical entity. Alveolar epithelial cells and alveolar macrophages are the first line of defense in the innate immunity against bacterial pathogens. Epithelial cells are known to release chemokines/cytokines that recruit and activate phagocytic cells. However, the signals sent from alveolar macrophages b...
Article
Exosomes are nano vesicles secreted by cells, and contain various molecules including protein, lipid and DNA/RNA. They are crucial mediators of the intercellular communication and serve as promising vehicles for drug delivery and gene therapy. Recently, accumulating evidence suggest that microRNAs (miRNAs) may serve as new and potentially powerful...
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Full-text available
Intercellular communications between lung epithelial cells and alveolar macrophages play an essential role in host defense against acute lung injury. Hyperoxia-induced oxidative stress is an established model to mimic human lung injury. We show that after hyperoxia-associated oxidative stress, a large amount of extracellular vesicles (EVs) are dete...
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Full-text available
Extracellular vesicles (EVs) are a group of heterogeneous, nano-sized structures surrounded by lipid bilayer membranes that are released by cells. Depending on their size and mechanisms of formation, EVs are often referred to as exosomes, microvesicles (MVs) and apoptotic bodies (AB). EVs are evolutionally conserved vesicles that mediate intercellu...
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Caenorhabditis elegans (C. elegans), a member of the phylum Nematoda, carries the evolutionarily conserved genes comparing to mammals. Due to its short lifespan and completely sequenced genome, C. elegans becomes a potentially powerful model for mechanistic studies in human diseases. In this mini review, we will outline the current understandings o...
Article
Full-text available
Lung epithelial cell apoptosis is an important feature of hyperoxia-induced lung injury. Death receptor-associated extrinsic pathway and mitochondria-associated intrinsic pathway both mediate the development of lung epithelial cell apoptosis. Despite decades of research, molecular mechanisms of hyperoxia-induced epithelial cell apoptosis remain inc...
Article
Full-text available
Lung epithelial cell death is a prominent feature involved in the development of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). Hyperoxia-induced ALI (HALI) is an established animal model mimicking human ARDS. Small non-coding RNAs such as microRNAs (miRNAs) have potent physiological and pathological functions involving mul...

Citations

... In this way, microvesicles can provide intercellular and interorgan communication by delivering miRNAs to influence transcription and alter homeostatic processes. [26] Canonical biogenesis and processing of human miRNA ( Figure 1) initiate in the nucleus when RNA polymerase Ⅱ transcribes the miRNA gene as a long hairpin structure called primary miRNA transcript (pri-miRNA). Subsequently, a microprocessor complex integrated by Drosha and DGCR8 processes the pri-miRNA to produce precursor miRNA (pre-miRNA) with a shorter stem-loop structure. ...
... MiR-185-5p has been reported to participate in the process of cell death in response to various cell stresses, including hypoxia/ ischaemia injury. 27,28,44 A recent study indicated that inhibition of miR-185-5p in endothelial cells contributed to the recovery of heart function after MI. 29 However, the exact mechanism by which miR- 49 In the present study, we also found that lncRNA FAF could regulate caspase-7 activity under hypoxia/ischaemia in NRCM ( Figure S3). However, more efforts are needed to understand its mechanism. ...
... Extracellular miRNAs could act as signalling molecules at the paracrine and endocrine levels to regulate a broad array of physiological processes [17,18]. Their aberrant expression plays a significant role in the onset and development of pathological conditions, including pulmonary diseases [19,20]. ...
... Studies have shown that some ncRNAs, including hY4 RNA, are selectively released into EVs, and that RNAbinding proteins are necessary for this selective sorting [20][21][22]. Based on this research, we sought to identify the RNA-binding protein that interacts with hY4F. ...
... Most qPCR methods quantify the nucleotide sequences with delta-delta-Ct and report the differential expression of the target nucleotide sequences as fold difference in the expression related to the expressed housekeeping sequence. A more precise absolute concentration of the investigated nucleotide sequence molecules can be quantitatively assessed if the qPCR measurement includes standard dilutions of known concentration by which samples' Ct values can be translated to the nucleotide sequence concentration (Lee et al., 2020). Nevertheless, the absolute qPCR quantification approach is not used in practice. ...
... Extracellular vesicles (EVs) transfer functional mediators to neighboring and distant recipient cells (12)(13)(14)(15). EVs are broadly classified into two categories, small particles (also known as Exos) and large particles (or microvesicles), distinguished by their intra-cellular origin (16,17). ...
... In this context, extracellular vesicles (EVs) have also been targets of this approach, shedding light on their heterogeneity in terms of content, function, origin and potentiality [6]. EVs are phospholipid bilayer-enclosed vesicles released by all cell types, and they can be found in several biological fluids such as saliva [7], breast milk [8], tears [9], bronchoalveolar lavage fluid [10], blood [11], urine [12], cerebrospinal fluid [13,14] and others. They can be classified according to their size, biogenesis and function, however they are mainly distinguished into three classes: exosomes, which form by inward invagination of the endosomal membrane, then released into the extracellular space by fusion of the so-called "multivesicular body (MVB)" with the plasma membrane and range from 20 to 150 nm in size; microvesicles or ectosomes, which originate ...
... Because caveolin-1, a lipid raft protein, is a well-known ATI cell marker, miRNA-rich EVs derived by ATIs are also enriched in this protein. As a result, caveolin-1 may become a biomarker of inflammation in bacterial lung infection [92]. ...
... MicroRNAs (miRNAs) are a type of highly conserved single-stranded RNA with a length of approximately 22 nucleotides that are involved in the physiological and patho-logical functions of a variety of diseases, including tuberculosis [15], ALI/ARDS [16], pulmonary fibrosis [17], hepatitis [18], cardiovascular diseases [18], and cancer [18]. MiRNAs mainly bind to the 3 -untranslated region (3 -UTR) of mRNA to control multiple pathways and various cellular processes, such as inflammatory-immune responses and cell-cell interactions [19]. ...
... The discovery of extracellular miRs in the blood, despite the abundant presence of RNases, led to the proposal of a scenario in which miRs are encapsulated in EVs (30,33,34) or form circulating ribonucleoproteins (35,36). A growing number of reports have established that many, if not all, of the effects of EVs are mediated by miR cargos (34,(37)(38)(39)(40)(41)(42)(43), which remain functional to regulate cellular behaviors of the recipient cells (44). Exo-mediated functional transfer of miRs has been reported with a broad range of downstream effects on recipient cells (45). ...