Hanchun Chen's research while affiliated with The American Society for Biochemistry and Molecular Biology and other places

Publications (42)

Article
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BRCA1 and BRCA2 are the two most commonly mutated tumor suppressor genes associated with hereditary breast cancer (BC). Also, mutations in TP53, PIK3CA, PTEN and AKT1 were observed at a high frequency in BC with their mutation spectrum exhibiting a subgroup particularity with enormous clinical significance in the prevention, classification and trea...
Article
CTSL is expressed by cancerous tissues and encodes a lysosomal cysteine proteinase that regulates cancer progression and SARS-CoV-2 entry. Therefore, it is critical to predict the susceptibility of cancer patients for SARS-CoV-2 and evaluate the correlation between disease outcomes and the expression of CTSL in malignant cancer tissues. In the curr...
Article
Furin is a proprotein convertase that activates different kinds of regulatory proteins, including SARS-CoV-2 spike protein which contains an additional furin-specific cleavage site. It is essential in predicting cancer patients’ susceptibility to SARS-CoV-2 and the disease outcomes due to varying furin expressions in tumor tissues. In this study, w...
Article
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TMPRSS2 (OMIM: 602060) is a cellular protease involved in many physiological and pathological processes, and it facilitates entry of viruses such as SARS‐CoV‐2 into host cells. It is important to predict the prostate's susceptibility to SARS‐CoV‐2 infection in cancer patients and the disease outcome by assessing TMPRSS2 expression in cancer tissues...
Article
USP8 is a deubiquitinating enzyme in the family of ubiquitin-specific proteases (USPs) which can remove ubiquitin from the substrate and protect the substrate from degradation. The upregulated or mutated USP8 becomes hyperactivated and stabilizes numerous oncogenes or proto-oncogenes leading to cancer progression and survival by activating multiple...
Article
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The ACE2 gene is a receptor of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) for COVID-19 (coronavirus disease 2019). To analyze the expression profiles and clinical significances for this gene in humans, RNA-seq data representing 27 different tissues were analyzed using NCBI; total RNA was extracted from different tissues of mouse a...
Article
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Unfortunately, as for the second institute name of first author Baixu Zhou, "Department of Gynecology and Obstetrics, Guangzhou Women and Children's Hospital, Guangzhou, Guangdong, China", should be "Department of Gynecology and Obstetrics, Guangdong Women and Children Hospital, Guangzhou, Guangdong, China".
Article
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Breast cancer is the most frequently diagnosed cancer and the leading cause of cancer‐related deaths in women worldwide. In this study, a large Chinese pedigree with breast cancer including a proband and two female patients was recruited and a familial history of breast cancer was collected by questionnaire. Clinicopathological assessments and neoa...
Article
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The mutations in patients with X-linked retinitis pigmentosa (xlRP) have not been well described in the Chinese population. In the present study, a five-generation Chinese retinitis pigmentosa (RP) family was recruited; targeted next-generation sequencing (TGS) was used to identify causative genes and Sanger sequencing for co-segregation. RNA-seq d...
Article
Herein, a turn-on fluorescence assay was introduced for alkaline phosphatase (ALP) detection based on ThT/G-quadruplex system. The basis of the method is that chelation of guanine bases at the binding sites by Ag+ blocks G-quadruplex formation and decreases the fluorescence intensity sharply. In the presence of ALP, ascorbic acid 2-phosphate (AAP)...
Article
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This study aimed to clone and characterize novel isoforms of the human SPATA3 gene. The isoforms of SPATA3 gene was cloned into pGMT vector using human testis cDNA as template, and Sanger sequencing was performed. Their characterizations and tissue-specific expression profiles were analyzed. The two novel isoforms were successfully cloned and depos...
Article
Prostate cancer (PCa) is the most common cancer diagnosed and the second most common cause of cancer‐related death in men worldwide. The current androgen deprivation therapy for PCa cannot fully cure this disease. Moreover, androgen receptor gene amplification and mutation are associated with PCa to develop castration‐resistant prostate cancer (CRP...
Article
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MicroRNA-34a (miR-34a), a tumor suppressor, has been reported to be dysregulated in various human cancers. MiR-34a is involves in certain epithelial-mesenchymal transition (EMT)-associated signal pathways to repress tumorigenesis, cancer progression, and metastasis. Due to the particularity of miR-34 family in tumor-associated EMT, the significance...
Article
Thioflavin T (ThT), a water-soluble fluorescence probe and the conventional dye for the detection of amyloid fibrils has recently been demonstrated to recognize and bind nucleic acids. It induces the formation of G-quadruplex (G4) structure and appears as a sensor by emitting enhanced fluorescence. Incorporation of G-rich sequences in the nucleic a...
Article
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As a tumor marker, carcinoembryonic antigen (CEA) is an effective indicator to evaluate the response to treatments of cancers. Therefore, it is necessary to find some sensitive and effective methods for CEA detection. Herein, we describe a facile method of CEA determination based on dsDNA-templated copper nanoparticles (CuNPs) coupled with an aptam...
Article
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Ochratoxin A (OTA) is one of the most common mycotoxins contaminating feed and foodstuffs. Therefore, a great deal of concern is associated with AFB1 toxicity. In this work, a fast and sensitive fluorescence aptamer biosensor has been proposed for the OTA assay. In the absence of OTA, the OTA aptamer can form a G-quadruplex structure with thioflavi...
Article
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Cervical cancer is one of the most common gynecological malignant tumors worldwide, for which chemotherapeutic strategies are limited due to their non-specific cytotoxicity and drug resistance. The natural product thymoquinone (TQ) has been reported to target a vast number of signaling pathways in carcinogenesis in different cancers, and hence is r...
Article
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In this work, we developed a facile fluorescence method for quantitative detection of human serum albumin (HSA) based on the inhibition of poly(thymine) (poly T)-templated copper nanoparticles (CuNPs) in the presence of HSA. Under normal circumstances, poly T-templated CuNPs can display strong fluorescence with excitation/emission peaks at 340/610...
Article
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Stargardt disease-4 (STGD4) is an autosomal dominant complex, genetically heterogeneous macular degeneration/dystrophy (MD) disorder. In this paper, we used targeted next generation sequencing and multiple molecular dynamics analyses to identify and characterize a disease-causing genetic variant in four generations of a Chinese family with STGD4-li...
Conference Paper
Cervical cancer is a major type of gynecological malignant tumor, of which the chemotherapeutic strategies are limited by its non-specific cytotoxicity and drug resistance. Thymoquinone (TQ), a natural product has been reported to target vast number of signaling pathways in carcinogenesis in different cancers, and hence regarded as a promising anti...
Article
The present work demonstrates a simple, rapid and label-free ATP detection method using a fluorescent aptasensor that is based on G-quadruplex formation. In the absence of ATP, the Thioflavin T (ThT) dye binds to the G-rich ATP aptamer and forms an ATP aptamer/ThT G-quadruplex complex, which results in high fluorescence intensity. Upon addition of...
Article
Background: PIK3CA gene encodes the p110 α catalytic subunit of the oncoprotein phosphatidylinositol 3-kinase (PI3 K) which regulates many biological processes such as cell proliferation, differentiation, migration and survival through the activation of various signaling pathways. Objective: In this study, we have investigated the possible somat...
Article
Here we report a new approach for label-free colorimetric assay of T4 polynucleotide kinase/phosphatase (PNKP) activity based on G-quadruplex/hemin DNAzyme. In the presence of T4 PNKP, the DNA primer with a 3’-phosphate can be dephosphorylated into a 3’-hydroxyl and initiate a primer elongation reaction to open the hairpin probe, and leading to rel...
Article
This work demonstrates a novel method for DNA methyltransferase (MTase) activity detection with a quencher-free molecular beacon (MB) probe based on exonuclease (Exo) III-assisted signal amplification. In the presence of Dam MTase and DpnI endonuclease, the elaborately designed hairpin substrate (MB1) was cleaved into two parts (part A and part B)....
Article
Regulation of gene expression by TRIM28 is not only by transcriptional repression but also by post-translational regulation. Here we report that TRIM28 promotes breast cancer cell metastasis through TRIM28-TWIST1-EMT pathway. We find that TRIM28 is highly expressed in metastatic cancer cell lines and advanced breast cancer tissues. The expression o...
Article
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TRIM28 regulates its target genes at both transcriptional and posttranscriptional levels. Here we report that a TRIM28-TWIST1-EMT axis exists in breast cancer cells and TRIM28 promotes breast cancer metastasis by stabilizing TWIST1 and subsequently enhancing EMT. We find that TRIM28 is highly expressed in both cancer cell lines and advanced breast...
Article
We have developed a new methodology for fluorescence turn-on detection of DNA methyltransferase (MTase) activity based on terminal deoxynucleotidyl transferase (TdT) using a thioflavin T probe. This method is highly selective and sensitive. The fluorescence intensity was direct proportion to Dam MTase concentration in the range from 0.1 to 8.0 U/mL...
Article
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The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random amplified polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplified region (SCAR) markers. These markers have been d...
Article
Traditional methods to assay polynucleotide kinase (PNK) activity are discontinuous, time-consuming and laborious. Here we report a new quencher-free approach for real-time monitoring of PNK activity using a 2-aminopurine probe. When the 2-aminopurine probe was 5'-phosphorylated by PNK, it could be efficiently degraded by lambda exonuclease to rele...
Article
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Objective: To identify 14 bacteria by sequencing the 16S rRNA gene and establish the basis for clinical application in the future. Methods: DNA samples of the 14 bacteria were extracted. The 16S rRNA genes were amplified by PCR and sequenced with common primers. The sequences of the 16S rRNA genes were aligned by online software Blastn in nucleo...
Article
Phosphorylation and dephosphorylation of DNA by polynucleotide kinase (PNK) takes an important role in DNA damage repair, replication and recombination. Traditionally, it is assayed by denaturing gel electrophoresis and autoradiography, which are tedious and not sensitive. We report on the development of a sensitive and simple method for PNK assay...
Article
In this research, we investigated the allele frequencies and forensic parameters of 21 non-, CODIS short tandem repeat (STR) loci (D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435 and D5S2500) among 523 unrelated,...
Article
Full-text available
[Available here: http://www.hindawi.com/journals/bmri/aip/570909] Hepatocellular carcinoma (HCC) is one of the most prevalent tumors worldwide. Interferon-α (IFN-α) has been widely used in the treatment of HCC, but patients eventually develop resistance. Interferon stimulating gene 15 (ISG15) is an ubiquitin-like protein transcriptionally regulate...
Article
Huwentoxin-IV (HWTX-IV, also named Mu-theraphotoxin-Hh2a) is a typical inhibitor cystine knot peptide isolated from the venom of Chinese tarantula Ornithoctonus huwena and is found to inhibit tetrodotoxin-sensitive (TTX-S) sodium channels from mammalian sensory neurons. This peptide binds to neurotoxin receptor site 4 located at the extracellular S...
Article
A novel fluorescent probe for adenosine triphosphate (ATP) assay based on DNA ligation is proposed in this article. This approach uses a novel smart probe, T4 DNA ligase, and two short oligonucleotides. In the presence of ATP, the T4 DNA ligase catalyzes the ligation reaction and the ligation product restores the fluorescence of the smart probe. Th...
Article
We have developed a new methodology for label-free highly sensitive telomerase activity assay using chemiluminescence imaging. This method can detect the telomerase activity from as little as 10 cultured cancer cells without PCR. Furthermore, telomerase inhibition is shown, demonstrating the potential for screening of telomerase inhibitors as antic...

Citations

... It was reported that SARS-CoV-2 can directly infect and injure many extrapulmonary organs, like the heart, kidney, liver, pancreas, and gastrointestinal tract [18][19][20] , usually with high CTSL expression levels. Besides, the mRNA expression level of CTSL is higher than angiotensin-converting enzyme 2 (ACE2), FURIN, and TMPRSS2 in human lung tissues 21 . Recently, we found that in SARS-CoV-2-infected patients, the circulating blood level of CTSL is highly correlated with the severity and course of COVID-19 symptoms. ...
... The brain is one of the organs that showed the highest furin expression levels [28], particularly in the regions of the cerebral cortex, hippocampus, and cerebellum, as high as that in the salivary gland [28]. Brain-derived neurotrophic factor (BDNF), one of the main substrates proteolytically activated by furin [29], is a key regulator of synaptic plasticity and cognitive function [30][31][32][33]. ...
... To facilitate risk-stratification and management of coronavirus 2019 (COVID-19), a better understanding of the factors associated with severe disease is necessary. In particular, several studies have focused on the androgen pathway to explain differences between men and women (5,6). Indeed, it has been recognized that SARS-CoV-2 binds to ACE2 to enter epithelial cells of the upper and lower respiratory tracts (7). ...
... Loss of USP8 leads to the subsequent suppression of proliferation, migration, and invasion, while inducing apoptosis of NSCLC cells [23,24]. In addition, as a deubiquitinase, USP8 improves the stability of oncoproteins by preventing ubiquitin-dependent degradation [25,26]. Yet, whether USP8 plays a regulatory role in PD-L1 expression and immune escape in NSCLC remains unclear and requires further investigation. ...
... ACE2 gene expression is also found in other respiratory disorders such as SARS, Middle East respiratory syndrome (MERS), and H1N1 influenza [54]. (Figure 3) [4,44,48]. This attack occurs through a series of mechanisms such as proteolysis, viral fusion with membrane, and entry mediated by ACE2 and transmembrane serine protease 2 (TMPRSS2) in some parts of the brain, CNS, PNS, and cerebrospinal fluid described through animal studies [see 55-58 for more details]. ...
... RAMP markers were, therefore, developed taking cognizance of the merits and demerits of the two marker systems. RAMP markers resolve to a large extent, the major challenges posed by microsatellite and RAPD markers (Liu et al., 2020). The RAMP technique uses a microsatellite matching primer which amplifies a section of isolated DNA with or without the activity of RAPD primers. ...
... In order to determine tissue-specificity, the ACE2 gene expression profiles were analyzed from RNA-sequencing (RNA-seq) data, performed on tissue samples from 95 human individuals representing 27 different tissues, using the NCBI program (https ://www.ncbi.nlm.nih.gov/ gene/59272 ) [21]. These data were collected from RNAseq normal tissues (BioProject: PRJEB4337; Publication: PMID 24309898). ...
... 22,23 In this context, evidences are accumulating about BRCA1/2 screening in specific populations, highlighting the differences between distinct geographical regions. [24][25][26][27] While reports exist about BC cohorts from western Sicily, fewer data are available about BRCA1/2 screening among eastern Sicily population. 28,29 We describe here the results of germline BRCA1/2 screening in BC patients from eastern Sicily, further correlating the presence of a BRCA1 or BRCA2 mutations with the main clinico-pathological features of these tumors. ...
... Novel CDH23 mutations in patients with USH1D and their relationships with genotypes/phenotypes are not well-documented in the Chinese population. Next-generation sequencing (NGS) provides a useful genetic diagnosis approach (Adams and Eng, 2018;Fu et al., 2019;Rahbaran et al., 2019). In the present study, novel missense compound heterozygous mutations of CDH23 are revealed in Chinese pedigree links with USH1D. ...
... (1) Target molecules directly affect the properties of AgNCs. (2) Target molecules alter the structure of DNA template to indirectly modulate the properties of AgNCs, which are often accomplished by constructing a aptamer-functionalized fluorescent AgNCs [85,86]. ...