Guillaume Barnoin's research while affiliated with Université Côte d'Azur and other places

... Keeping these requirements in mind, the 6-EMT label was proposed, which is composed of a 3-methoxychromone (3MC) core unit branched by two moieties, namely, an ethynyl group at position 6 and a five-membered ring (thiophene) at the 2-position ( Figure 1B and Scheme 2). Besides blocking the ESIPT reaction [68][69][70][71], the presence of the methyl group was previously shown to affect the planarity of the fluorochrome and thus quenches the fluorescence of 2-thienyl-3MC [25]. As the terminal alkyne has little impact on photophysics-because it is not electronically coupled with the acceptor group (4-ketone)-its role is exclusively related to the subsequent labeling of NAs by the post-synthetic click reaction. ...

... In a previous study from our laboratories, a novel xanthene-based reagent for the fluorogenic derivatization of primary amines was designed, synthesized and characterized, and then its application to the derivatization of cyclopropylamine was examined [40], with the latter acting a model compound. The reagent is selective for primary amines, as the reaction products with secondary amines exhibit no shift in UV absorption and fluorescence. ...

... 17 MLCT-emissive phosphorescent RPCs hold significant potential as FRET donors, where the low energy and large Stokes shifts of MLCT phosphorescence (typically >150 nm) would be advantageous in addressing current limitations arising from the use of two organic fluorophores, which include poor signal-to-noise and spectral bleed through. 24 Despite this, few studies have examined RPCs in FRET bioassays. Work to date has included a [Ru(bpy) 3 ] 2+ -based enzyme-cleavable sensor 25 and DNA sequences covalently labeled with RPCs to examine DNA− DNA assembly. ...

... These agents can promote ROS generation and inhibit the Nrf2-ARE pathway. Downregulation of the Nrf2 pathway in the MCF-7 Breast cancer cell line induces sensitivity to chemotherapy drugs [171]. Micro-ribonucleic acids (miRNAs), a collection of non-coding RNAs, exhibit a fundamental role in the regulatory mechanism in organisms. ...

... A tetrazolium-based MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was used to determine cell viability, as previously described [90]. After 72 h of treatment at 37 • C and 5% CO 2 , cells were incubated with 25 µL MTT solution (5 mg/mL; Sigma-Aldrich) for 4 h. ...

... This represents the case of 2-aminopurine, which minimizes the structural perturbation of the nucleic acid [13] yet suffers from a low fluorescence quantum yield. This strategy has also been used for the design of the dye shown in Fig. 1 i.e. 2-thienyl-3-hydroxychromone (3HC) [14], referred as M hereafter. ...