Guangyin Zhen’s research while affiliated with East China Normal University and other places

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Publications (134)


Exploring the chemical behaviors of dissolved organic matter to thermal hydrolysis temperature at the molecular level and its fate in anaerobic membrane bioreactor
  • Article

April 2025

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7 Reads

Water Research

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Teng Cai

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Yizhi Zhang

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Guangyin Zhen






Removal of the soluble COD from the sludge under different pH conditions (a, b, c, d)
(a & b) Removal of sludge soluble COD by different dosages of Phanerochaete chrysosporium (c & d).
Changes in the soluble COD of sludge samples from experimental groups
UV254 changes in sludge samples from experimental groups
Infrared mapping of the sludge from different experimental groups

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Studies on the treatment of anaerobically digested sludge by white-rot fungi: evaluation of the effect of Phanerochaete chrysosporium and Trametes versicolor
  • Article
  • Full-text available

January 2025

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29 Reads

Microbial Cell Factories

Background The composition of anaerobically digested sludge is inherently complex, enriched with structurally complex organic compounds and nitrogenous constituents, which are refractory to biodegradation. These characteristics limit the subsequent rational utilization of resources from anaerobically digested sludge. White-rot fungi (WRF) have garnered significant research interest due to their exceptional capacity to degrade complex and recalcitrant organic pollutants. However, the exploration of WRF in the context of sludge treatment remains an under-investigated area within the scientific community. The present investigation explores the application of WRF in the treatment of anaerobically digested sludge, offering a novel approach for the valorization of sludge resources. Results In this study, WRF enzymes, manganese peroxidase (MnP) and lignin peroxidase (LiP), exhibited sustained high activities of approximately 102 U/L and 26 U/L, respectively, within the anaerobically digested sludge under a controlled pH of 5.5 within the growth system. These conditions were found to significantly enhance the treatment efficacy of the anaerobic sludge. The removal of soluble chemical oxygen demand (COD) and Total COD by Trametes versicolor powder was better than that of Phanerochaete chrysosporium powder. The treatment of sludge samples with WRF, specifically Phanerochaete chrysosporium powder, resulted in a significant reduction of ultraviolet radiation (UV254). Fourier-transform infrared spectroscopy (FTIR) analysis revealed that the application of Trametes versicolor powder exerted a notably pronounced impact on the functional groups present in sludge samples. Specifically, there was a significant decrease in the peak intensities corresponding to the C-O bonds, indicative of saccharide degradation, alongside an observable increase in the intensities of amide peaks, which is suggestive of protein synthesis enhancement. Microbial community analysis demonstrated that Phanerochaete chrysosporium was the predominant fungal species, exerting a significant regulatory role within the sludge ecosystem. Conclusion In conclusion, this research furnishes a robust scientific foundation for the utilization of WRF in the treatment of anaerobic digestion sludge. It elucidates the fungi’s capacity to ameliorate the physicochemical attributes and microbial community composition within the sludge. Furthermore, the study offers a certain reference for the subsequent use of WRF in the treatment of other types of sludge.

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Citations (43)


... Biological methods: Biological processes employ microbial metabolism to transform nitrogen and phosphorus in the liquid phase into microbial biomass. Typically, the aqueous product is mixed with a culture medium containing specific microorganisms under suitable conditions, enabling them to absorb and incorporate N and P into their biomass [174][175][176][177]. While biological methods are environmentally friendly and cost-effective, they require longer processing times and the careful cultivation and management of microorganisms. ...

Reference:

Advances in Hydrothermal Carbonization for Biomass Wastewater Valorization: Optimizing Nitrogen and Phosphorus Nutrient Management to Enhance Agricultural and Ecological Outcomes
Microalgae biofilm barrier to assist the simultaneous nitrogen and phosphorus removal in anammox: Molecular mechanism on sludge granulation and microbial metabolism
  • Citing Article
  • November 2024

Chemical Engineering Journal

... The ATP activity and cyt c activity were measured using commercial test kits (Beijing Solarbio, Beijing, China). Furthermore, EPS was extracted using a modified oscillation-ultrasound combined cationic resin extraction method and analyzed for the components (polysaccharides, proteins, lipids, humic acids, and nucleic acids) by the standard substance method [25]. In addition, enzyme activities were measured using seed sludge as a control group to comparatively analyze the changes in cell viability after microplastic exposure. ...

Sludge bound-EPS solubilization enhance CH4 bioconversion and membrane fouling mitigation in electrochemical anaerobic membrane bioreactor: Insights from continuous operation and interpretable machine learning algorithms
  • Citing Article
  • August 2024

Water Research

... With enterprises' current emphasis on their operations' costeffectiveness and low-carbon emissions, biological treatment technologies are increasingly favored for their low-carbon footprint Abbreviations: HSOW, hypersaline organic wastewater; AOPs, advanced oxidation processes; ZLD, zero liquid discharge; ROS, reactive oxygen species; DIET, direct interspecies electron transfer; PAC, powdered activated carbon; CNTs, carbon nanotubes; BES, bioelectrochemical system; SA, sodium alginate; PVA, polyvinyl alcohol; EPSs, extracellular polymeric substances; EMBRs, electric field-coupled membrane bioreactors; SMF, static magnetic field; PHA, polyhydroxyalkanoate; COD, chemical oxygen demand; TDSs, total dissolved solids; TN, total nitrogen; IOMNPs, iron oxide magnetic nanoparticles; EMCI, embedding microbial cell immobilization; UV, ultraviolet; WWTPs, wastewater treatment plants; KPIs, key performance indicators; IoT, Internet of Things; UF, ultrafiltration; RO, reverse osmosis. and minimal amounts of secondary pollution compared to those of alternatives [13,14]. However, the high salinity and toxicity of pharmaceutical and chemical wastewater can severely inhibit microbial activity [15]. ...

Natural defence mechanisms of electrochemically active biofilms: From the perspective of microbial adaptation, survival strategies and antibiotic resistance
  • Citing Article
  • July 2024

Water Research

... Our results revealed that in addition to abiotic factors, biotic factors-particularly the diversity of the bacterial communityexert an extremely significant and strong influence on the complexity of its network. This implies that the presence or absence of specific bacterial community members not only affects their interactions but also plays a pivotal role in the formation of the entire network Yu et al., 2024). Although the diversity of fungal communities exhibited significant differences across tree ages, there was no notable effect on the complexity of their networks. ...

Elevational dynamics in soil microbial co-occurrence: Disentangling biotic and abiotic influences on bacterial and fungal networks on Mt. Seorak
  • Citing Article
  • May 2024

Soil Ecology Letters

... Microbes can utilize various electron donors (energy-provided OM) and acceptors (i.e., As(V) and Fe(III)) for metabolism, enhancing their survival in anoxic environments (Liu et al., 2024;Lu et al., 2021). The surface properties of microbes, such as charge properties and hydrophobicity of cell membranes, can affect their interactions with electron acceptors, improving electron transfer efficiency (Cai et al., 2024). The formation of biofilms also enhances electron transfer efficiency, allowing them to effectively participate in the electron shuttling process (Dhar et al., 2017). ...

Engineering hybrid conductive electrochemically active biofilms enable efficient interfacial electron transfer and syntrophic carbon metabolism
  • Citing Article
  • March 2024

Chemical Engineering Journal

... Many investigations on DF by anaerobic fermentative bacteria, such as Escherichia coli [36][37][38], Clostridium [39][40][41][42][43][44][45], and Enterobacter [46][47][48][49], have been conducted. Numerous authors have documented H2 production from mixed microflora derived from various sources, including heat-treated anaerobic sludge [50][51][52] and cow dung [53][54][55][56][57], has been documented by numerous authors. Several elements, such as the kind of organism, the metabolic pathway taken, the type of substrate utilized, and the final products generated, influence how much H 2 is produced during DF. ...

Hydrogen production promotion and energy saving in anaerobic co-fermentation of heat-treated sludge and food waste

Environmental Science and Pollution Research

... The drivers of HA production in composting are therefore of interest to the industry [16,21,22]. As straw is a lignocellulosic material that is difficult to chemically degrade, many pre-treatments are required to modify the structure of the plant cell walls to enable microbial utilization of the material [13,18]. ...

Effect of municipal sludge-based biochar produced at different pyrolysis temperatures on humification and oxytetracycline degradation of pig manure composting
  • Citing Article
  • October 2023

The Science of The Total Environment

... The substrate characteristics, such as the moisture content, pH, chemical composition and particle size, also affect biomethane production through methanogenesis [8,9]. A wide range of microbial communities are responsible for the methanogenesis process during anaerobic treatment [10], including fermentative and syntrophic acetogenic bacteria and methanogenic archaea [11]. These microorganisms contribute to the degradation of complex molecules into simpler compounds during the fermentative, acidogenic, acetogenic, and methanogenic stages [12,13]. ...

Bioelectrochemical anaerobic membrane bioreactor enables high methane production from methanolic wastewater: Roles of microbial ecology and microstructural integrity of anaerobic biomass
  • Citing Article
  • July 2023

Chemosphere

... High removal efficiencies of COD were consistently achieved in both AnMBR-MEC and AnMBR systems (> 90%) even with long operation periods, and in most cases the electrochemical-AnMBR outperformed the conventional system reaching removal rates with COD > 95% [36][37][38]44,52,62,66]. Even in pilot-scale studies with high-strength pesticides wastewater influents, AnMBR-MEC maintained stable average COD removal rates ranging from over 70% to around 81% [42,43]. ...

Impact of sandwich-type composite anodic membrane on membrane fouling and methane recovery from sewage sludge and food waste via electrochemical anaerobic membrane bioreactor
  • Citing Article
  • May 2023

Bioresource Technology

... hydrolysis, electron transfer, and electromethanogenesis(Zhao et al. 2021;Zhen et al. 2023).Shorter HRTs may be favorable for electrochemical enhancement with AnEMBRs more owing to a higher organic loading rate. CH4 production in AnEMBRs can occur primarily through hydrogenotrophic methanogenesis because the detection of acetoclastic methanogens in solution or on the electrode is limited). ...

Enhanced co-digestion of sewage sludge and food waste using novel electrochemical anaerobic membrane bioreactor (EC-AnMBR)
  • Citing Article
  • March 2023

Bioresource Technology