Guangjie Han's research while affiliated with Jiangsu Academy of Agricultural Sciences and other places

Publications (13)

Article
Full-text available
Intestinal bacterial flora plays an important role in the nutrition, physiology, and behavior of herbivorous insects. The composition of gut microbiota may also be affected by the food consumed. Cnaphalocrocis medinalis is an oligophagous pest, feeds on rice leaves almost exclusively and causes serious damage to rice in Asian countries. Using antib...
Article
Bacillus thuringiensis subsp. israelensis (Bti) has been proven to efficiently control mosquitoes, of which many species are important vectors of human disease. The larvicidal action is attributed to the parasporal crystals formed in the sporulating cells, and released upon cell autolysis. In this study, a sporulation‐specific cwlC gene that encode...
Article
Full-text available
Background Short interspersed nuclear elements (SINEs) belong to non-long terminal repeat (non-LTR) retrotransposons, which can mobilize dependent on the help of counterpart long interspersed nuclear elements (LINEs). Although 234 SINEs have been identified so far, only 23 are from insect species (SINEbase: http://sines.eimb.ru/ ). Results Here, f...
Article
Cnaphalocrocis medinalis granulovirus (CnmeGV) is a potential microbial agent against the rice leaffolder. Innate immunity is essential for insects to survive pathogenic infection. Therefore, to clarify the immune response of Cnaphalocrocis medinalis to the viral colonization, the gene expression profile of C. medinalis infected with CnmeGV was con...
Article
Full-text available
All major insecticidal genes of Bacillus thuringiensis var. israelensis (Bti) are controlled by the sporulation-specific sigma factor Sigma E (sigE), while sigE is negatively regulated by Sigma K (sigK). Therefore, knocking out sigK plays an important role in regulating the expression of insecticidal genes in Bti. A sigK deletion mutant of B. thuri...
Article
Full-text available
Background: Helitrons play an important role in shaping eukaryotic genomes due to their ability to transfer horizontally between distantly related species and capture gene fragments during the transposition. However, the mechanisms of horizontal transfer (HT) and the process of gene fragment capturing of Helitrons still remain to be further clarif...
Article
Objective: To explore the feasibility of using enhancin as synergist to Bacillus thuringiensis (Bt), the truncated fragments of enhancin gene from Pseudaletia unipuncta granulovirus (PuGV-Ps) were optimized and the enhancing effects were studied. Methods: Based on bioinformational analysis of the function domain of PuGV-Ps enhancin, the prokaryo...
Article
Control of . Bemisia tabaci has depended primarily and heavily on insecticides, especially neonicotinoids. The novel sulfoximine insecticide sulfoxaflor exhibits high potency against a broad range of sap-feeding insect species, including those resistant to neonicotinoids. The resistance levels of Q-biotype . B. tabaci field strains collected from 8...
Article
Background: The rice stem borer, Chilo suppressalis (Walker), is one of the most damaging rice pests in the world. The sublethal effects of chlorantraniliprole on development,reproduction, and mRNA expression levels of vitellogenin gene (CsVg) in C. suppressalis were investigated. Results: Exposure of sublethal concentrations of chlorantranilipr...
Data
Phylogenetic analysis using the predicted amino acid sequences of the partial polh/gran, lef-8 and lef-9 genes. The NJ tree is shown. Numbers above or below the nodes are bootstrap values showing the statistical reliability of bootstrapping with 1,000 replicates. (DOCX)
Article
Full-text available
Cnaphalocrocis medinalis is a major pest of rice in South and South-East Asia. Insecticides are the major means farmers use for management. A naturally occurring baculovirus, C. medinalis granulovirus (CnmeGV), has been isolated from the larvae and this has the potential for use as microbial agent. Here, we described the complete genome sequence of...
Data
Analysis of putative CnmeGV ORFs with homologous ORFs from databases of NCBI. ♦Nucleotide position of putative ORFs and the orientation of transcription are shown in arrows. The gene names are shown in the second column and italicized. The symbols represent the following; *Calculation of amino acid identities (%) in homologous ORFs was based on Bla...
Article
Plutella xylostella granulovirus (PxGV) and Bacillus thuringiensis (Bt) are both entomo-pathogens to the diamondback moth, Plutella xylostella (L.). The purpose of the present study was to measure the effect of the combination of PxGV and Bt at sublethal dosages on the development and mortality of diamondback moth in a laboratory setting. Bt and Px...

Citations

... While SINEs depend on other TEs for their retrotransposition, evidence of SINE HT is present in the literature. The relatively young 5S RNA-derived SINE PxSE4 shows evidence of HT in lepidopteran insects [51], and novel HaSE SINEs show signatures of HT in the cotton bollworm, Helicoverpa armigera [52]. Indicators of HT have also been found for a SINE shared by reptiles and mammals and the SmaI SINE family in salmonid fishes [53,54]. ...
... The deletion of cwlC or sigK in the Bacillus thuringiensis var. israelensis (Bti) strain did not block mother cell lysis (Xu et al., 2020;Huang et al., 2022), because other cell wall hydrolases, like CwlE, are involved in mother cell lysis in Bti (Huang et al., 2022). However, the deletion of the mclX homolog gene in Bti also did not block mother cell lysis (Supplementary Figure 6). ...
... However, many studies including the recent study of Tc1/Mariner TEs in the genomes of nematodes reported significant inconsistency of TEs with their molecular phylogeny as compared to mitochondrial and other single non-transposable genes from the same genome, which indicates MLEs had evolved independently of host speciation event 19,[39][40][41][42][43][44] . We also observed patchy distribution of MLEs in our studied species irrespective of the host phylogenies, which could indicate the independent evolution of MLEs to some degree, also reported by previous studies [45][46][47][48] . For instance, MLEs from Irritans subfamily were identified in four species but absent in three of the studied species, i.e., Floraphis choui, Kaburagia rhusicola and Melaphis rhois (see Table 1), while the distribution of other MLEs among the species were very irregular (see Fig. 1B), irrespective of Rhus gall aphid's phylogeny 22 . ...
... [19][20][21][22] Although these pests showed crossresistance to other neonicotinoids, DTF has been reported to be effective against IMI-resistant strains. [23][24][25][26][27][28] 2.2. Binding affinity of DTF to cytochrome P450s that confer IMI resistance Homology models of CYP6CM1 and CYP6ER1 were built based on human CYP3A4 (PDB entry code 4D75). ...
... As a result, the female body shuts down less vital processes during infection to save energy for more important tasks. This trade-off strategy is common in insects encountering various stressful situations (Kodrík, 2008;Zhang et al., 2014;Huang et al., 2016). ...
... Besides, the lef2 gene-based phylogeny in HearNPV was demonstrated as a useful parameter to interpret the ancestral relationship and evolutionary history of the baculovirus [69]. The phylogenetic tree represented in our study based on the core genes of the SpobNPV-Manipur isolate provided a clear evolutionary classification between the four genera of the baculovirus (Alphabaculovirus, Betabaculovirus, Gammabaculovirus and Deltabaculovirus) likewise the phylogeny of CapoNPV [65] and CnmeGV [70]. The species of the alphabaculovirus genera were further subdivided into two groups (group I and group II). ...
... As an alternative, PlxyGV has been tested for the control of the pest [16,19,20,23]. A registered PlxyGV biopesticide has been commercialized and used in large scale for the control of diamondback moth in China and Malaysia since 2008 [24][25][26][27][28][29]. Laboratory experiments have also been done to characterize PlxyGV morphology, histopathology, in vitro replication in cell culture, and molecular biology [17,[30][31][32][33][34][35][36]. ...