Gregory C. Gibbons's research while affiliated with Carlsberg Research Centre and other places

Publications (4)

Article
Full-text available
The N-terminal amino acid sequence of the small subunit of ribulose-1,5=disphosphate carboxylase from the amphidiploidNicotiana tabacum contains two polymorphisms. From examination of the equivalent sequences in the putative parent speciesNicotiana sylvestris andtomentosiformis it is concluded that the amphidiploidNicotiana tabacum has inherited tw...
Article
Full-text available
This paper presents a simple and rapid procedure for the isolation of ribulose-1,5-diphosphate carboxylase from leaves. By column chromatography on Sephadex G-25 and Sepharose 6B combined with concentration by ultrafiltration high yields of undenatured protein are obtainable in less than one working day. RudP carboxylase purified from barley in thi...
Article
Summary The N-terminal sequence of the small subunit of Fraction I protein isolated from tobacco was investigated, using an automatic protein sequencer. The amino acid sequence of the first 21 residues is presented.

Citations

... The amino acid sequences obtained are summarized in Figure 5. All sequences were known for tobacco and allowed the unambiguous identification of single subunits: the PsaH protein of Photosystem I ( Obokata et al. 1993), the small subunit of RubisCO ( Gibbons et al. 1975), the-subunit of the ATP synthase ( Deno et al. 1983), cytochrome f, the FeS protein, cytochrome b 6 and the subunit IV of the b 6 f complex ( Madueno et al. 1992;Shinozaki et al. 1986) and the cytochrome b-559 subunit of Photosystem II ( Carrillo et al. 1986). ...
... Nicotiana tabacum which is likely to be the result of a fusion of Nicotiana sylvestris and Nicotiana tomentosiformis (Strobaek et al. 1976). This type of duplication however, can give rise only to dispersed multi-gene families. ...