Gilbert Van Stappen's research while affiliated with Ghent University and other places

Publications (117)

Article
This research was done to investigate the application of halophilic bacteria in a biofloc production system to grow Artemia franciscana in the laboratory, in which part of a standard microalgae feeding regime was replaced by bioflocs. Bioflocs were produced according to a standard procedure, using rice bran and vinasse as carbon sources, a commerci...
Article
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Determining how and how often asexual lineages emerge within sexual species is central to our understanding of sex-asex transitions and the long-term maintenance of sex. Asexuality can arise “by transmission” from an existing asexual lineage to a new one, through different types of crosses. The occurrence of these crosses, cryptic sex, variation in...
Article
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Green fluorescent protein (GFP) has been used extensively for in situ animal studies that follow up bacterial infection under epifluorescence microscopy. It is assumed that GFP is acting as a “neutral” protein with no influence on the bacterial physiology. To verify this hypothesis, the virulence of Vibrio splendidus ME9, Vibrio anguillarum NB10, a...
Article
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Aquaculture has the potential to sustainably meet the growing demand for animal protein. The availability of water is essential for aquaculture development, but there is no knowledge about the potential inland water resources of the Rwenzori region of Uganda. Though remote sensing is popularly utilized during studies involving various aspects of su...
Article
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Background Brine shrimp Artemia have an unequalled ability to endure extreme salinity and complete anoxia. This study aims to elucidate its strategies to cope with these stressors. Results and discussion Here, we present the genome of an inbred A. franciscana Kellogg, 1906. We identified 21,828 genes of which, under high salinity, 674 genes and un...
Article
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As the immune system is not fully developed during the larval stage, hatchery culture of bivalve larvae is characterized by frequent mass mortality caused by bacterial pathogens, especially Vibrio spp. However, the knowledge is limited to the pathogenesis of vibriosis in oyster larvae, while the immune response to pathogenic microorganisms in this...
Technical Report
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inadequate training of personnel who can tackle fast-evolving production systems and technologies geared towards sustainable aquaculture development has hampered the growth of the sector. Currently, the general aquaculture training landscape in Kenya and curricula are fragmented, do not meet labour market needs and are partially duplicative and som...
Preprint
Full-text available
Determining how and how often asexual lineages emerge within sexual species is central to our understanding of sex-asex transitions and the long-term maintenance of sex. Asexuality can arise "by transmission" from an existing asexual lineage to a new one, through different types of crosses. The occurrence of these crosses, cryptic sex, variation in...
Technical Report
Full-text available
The framework aims to tackle some of the perennial challenges in the aquaculture educational landscape including; limited knowledge, skills and innovative capacity to operationalize and commercialize technological advancements in fish production such as culture systems, fish breeding and genetics; fish nutrition, health management and post-harvest...
Preprint
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The climate is currently warming fast, threatening biodiversity all over the globe. Adaptation is often rapid when the environment changes quickly, but for climate warming very little evidence is available. Here, we investigate the pattern of adaptation to an extreme +10°C climate change in the wild, following the introduction of brine shrimp Artem...
Article
A number of single-nucleotide polymorphisms (SNPs) in heat shock protein 70 (HSP70) family genes have been reported to be associated with stress tolerance. In this study, the presence of SNPs in either the ArHSP70 and ArHSC70 genes (heat shock cognate gene) was verified in 2 Artemia franciscana populations, either a control population (CF12) or a p...
Article
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A cross-sectional study was undertaken to examine the aquaculture extension service in Kenya. Primary data was collected using a semi-structured questionnaire administered to 292 randomly selected fish farmers and 56 extension officers in different counties in Kenya. Descriptive statistics mainly mean and frequencies were computed to determine key...
Article
The brine shrimp Artemia is a widely used cost-effective diet in aquaculture. In many Artemia studies, e.g., in a quality assessment of Artemia hatching, an automated method for detecting and counting the Artemia objects in images would be highly desired. However, there are few such works in literature. Moreover, it is very challenging to separate...
Article
Bacteria, especially Vibrio spp., are largely responsible for larval diseases in shellfish aquaculture. Despite the severe impact of bacterial diseases on larval health, only a few direct studies have characterized the pathogenesis of vibriosis in bivalve larvae. To clarify the onset and advancement of vibriosis in blue mussel Mytilus edulis larvae...
Article
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The organogenesis of the digestive system was described in the African lungfish (Protopterus aethiopicus) from 6 days post‐hatching (6 DPH) to 17 days post‐hatching (17 DPH) reared at 27°C. To elucidate the position of the gastrointestinal tract in relation to the neural tube, notochord and yolk sac at 6 DPH, and to the vertebral column, lungs and...
Article
The brine shrimp Artemia is of considerable economic importance in fish and shellfish larviculture. The quality of the Artemia product, differs, in terms of hatching and biometric characteristics, from strain to strain and from location to location. The same applies for their nutritional value which is not constant but varies among strains and with...
Article
Aquafeeds contain protein ingredients such as fishmeal and soybean meal, yet their production puts pressure on the environment. Finding novel protein sources such as dried microbial biomass produced on recovered or renewable resources, so-called single-cell protein or microbial protein, can contribute to a more sustainable aquaculture industry. New...
Article
Archaea, the third domain of life, are usually overlooked in aquaculture. Among them, the genus Haloferax is characterized by its tolerance for a wide salinity range and flexibility in using different organic substrates for cell growth. In order to clarify if Archaea can be predominant in bioflocs under certain conditions, Haloferax sp. was inocula...
Article
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The genus Artemia Leach, 1819 is a cosmopolitan halophilic crustacean, consisting of bisexual species and obligate parthenogenetic populations. Asia is rich in Artemia biodiversity. More than 530 Artemia sites have been recorded from this area and more than 20 species/subspecies/variety names have been used for them. There exist various problems in...
Preprint
Full-text available
Purple non-sulfur bacteria (PNSB) biomass is an emerging alternative protein source, yet research of PNSB as added-value protein ingredient is limited. This research aimed to study the use of PNSB as protein source for shrimp and investigate the shrimp's tolerance against Vibrio and ammonia stress. A 28-day shrimp feeding trial was performed with R...
Article
The brine shrimp Artemia, a small crustacean zooplankton organism, is universally used as live prey for larval fish and shrimps in aquaculture. In Artemia studies, it would be highly desired to have access to automated techniques to obtain the length information from Artemia images. However, this problem has so far not been addressed in literature....
Article
Archaea have been the most overlooked and enigmatic of the three domains of life for decades. Knowledge of key ecological interactions such as trophic links between this domain and higher level organisms remains extremely limited. The co-occurrence of halophilic Archaea (haloarchaea) and the non-selective filter feeder, brine shrimp Artemia under t...
Article
The brine shrimp Artemia is a highly required, convenient and cost‐effective live food used in fish and shellfish larviculture. Its cysts, originating from a limited number of inland salt lakes, are traded worldwide. Over the past decades, Artemia pond production in solar saltworks, integrated with salt production, has emerged as an alternative to...
Article
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Urmia Lake, the largest natural habitat of the brine shrimp Artemia urmiana, has progressively desiccated over the last two decades, resulting in a loss of 80% of its surface area and producing thousands of hectares of arid salty land. This ecological crisis has seriously affected the lake's native biodiversity. Artemia urmiana has lost more than 9...
Article
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Thus far, only one gene from the heat shock protein 70 (HSP70) family has been identified in Artemia franciscana. Here, we used the draft Artemia transcriptome database to search for other genes in the HSP70 family. Four novel HSP70 genes were identified and designated heat shock cognate 70 (HSC70), heat shock 70 kDa cognate 5 (HSC70-5), Immunoglob...
Article
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The use of stable isotope (SI) labelling and tracing of live diets is currently considered one of the most comprehensive tools to detect their uptake and assimilation by aquatic organisms. These techniques are indeed widely used in nutritional studies to follow the fate of specific microbial dietary components, unraveling trophic interactions. Neve...
Article
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Highly variable environmental conditions can put the survival of inhabitants of temporary pools at high risk, as they may not complete their life cycle. Different evolutionary responses can prevent the extinction of the inhabitants of ephemeral wetland habitats. One specific mode of response to environmental changes is bet-hedging as risk-spreading...
Article
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Cellular quiescence, a reversible state in which growth, proliferation, and other cellular activities are arrested, is important for self-renewal, differentiation, development, regeneration, and stress resistance. However, the physiological mechanisms underlying cellular quiescence remain largely unknown. In the present study, we used embryos of th...
Article
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The brine shrimp Artemia is an interesting experimental system for studies of developmental processes. Hatching of dormant cysts gives rise to shrimp larvae called nauplii, characterized by numerous naupliar stages representing the first forms of brine shrimp life cycle. Here combined Thin Layer Chromatography (TLC) and Matrix-Assisted Laser Desorp...
Article
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The complete mitochondrial genome of Artemia sinica was obtained using the next-generation sequencing (NGS) method. The mitochondrial genome is a circular molecule of 15,689 bp in length, with the typical structure of 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs) and 2 ribosomal RNA genes, and a non-coding control region (CR). The b...
Article
Artemia cysts are highly demanded by the aquaculture industry to be hatched into nauplii and used as live food for larvae of most farmed fish and shellfish species. In recent years, pond production of Artemia cysts has been proposed as an effective measure to reduce the high dependence of the industry on cysts harvested from wild populations. Nonet...
Article
Habitat salinity is a major abiotic factor governing the activity, physiology, biology and distribution of aquatic animals. Salinity changes cause salt stress, affecting crustaceans reared in aquaculture both on an ecological and economic level. Current salt stress research in aquatic animals is mainly focused on salt stress in the gills at relativ...
Article
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Seasonal Artemia pond production has been practiced for several decades by rural salt producing communities in the Mekong Delta, Vietnam, with more success than elsewhere in the world. In spite of this success, yields have been stagnating for a long time due to suboptimal feeding conditions. As feeding is one of the most important factors affecting...
Article
Brine shrimp Artemia, the most common live food organism used in larviculture, can reproduce either oviparously (production of dormant cysts) or ovoviviparously (direct production of nauplii), depending on environmental conditions. Ovoviviparous Artemia nauplii have seldom been considered as a source of live food in aquaculture, partly due to the c...
Data
https://www.researchgate.net/publication/325660897; Belgium, Uganda, publication of Sergei has new citation, 2018: Basics of the molecular-ecological mechanism of water quality formation and ; Cited by scientists from Belgium (Gent University) and Uganda; https://5bio5.blogspot.com/2018/06/citat.html This publication of Sergei has a new citation, 2...
Article
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Colorimetric analysis is commonly used to quantify P availability in aquatic environments. However, it may not be adequate to quantify the bioavailability of dissolved organic P compounds. This study aimed to investigate to what extent the colorimetrically measurable P fractions can be utilized by algae, with a particular emphasis on organic P comp...
Article
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Aquaculture production is predicted to increase sharply. In this regard, live feed plays a crucial role in the larval phase of many aquaculture organisms. Hence, a persistent concern in aquaculture is to find low‐cost and eco‐friendly feed sources to culture live feed organisms. Branchinecta orientalis (G. O. Sars 1901), a fresh/brackish water fair...
Article
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Ugandan aquaculture is in the process of development; however, it requires access to an affordable live food source, such as brine shrimp Artemia. This study fits within a broader feasibility study of domestic Artemia production in salt lakes. Since Uganda is a landlocked country, the only opportunity for live water food sources lies in the salt la...
Article
Effects of live and dry feeds on intestinal morphology and growth of African catfish, Clarias gariepinus, larvae (Burchell, 1822) were investigated from 2 days post hatching (dph) to 6 dph and 11 dph, respectively. Feeding trials were carried out at Fleuren and Nooijen hatchery, Netherlands, in glass tanks connected to a RAS system. Five test diets...
Article
The variable quality and high price of Artemia (Leach 1819) cyst products, used worldwide as live food, motivate aquaculturists to find an appropriate alternative, especially for fresh/brackish water organisms. In this study, Branchinecta orientalis (G. O. Sars 1901), a common fairy shrimp in north-western Iran, was reared for 15 days using effluen...
Article
The sex of relatively primitive animals such as invertebrates is mostly determined by environmental factors and chromosome ploidy. Heteromorphic chromosomes may also play an important role, as in the ZW system in lepidopterans. However, the mechanisms of these various sex determination systems are still largely undefined. In the present study, a Ma...
Article
In arthropods, mature females under certain conditions produce and release encysted gastrula embryos that enter diapause, a state of obligate dormancy. The process is presumably regulated by diapause hormone (DH) and diapause hormone receptor (DHR) that were identified in the silkworm, Bombyx mori and other insects. However, the molecular structure...
Article
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Microbial community management in aquaculture creates benefits at the nutritional as well as at health level for cultured species. In addition, in case of biofloc application, it allows to link species at different trophic levels, making bioflocs the potential link in integrated multispecies aquaculture.
Article
Bacterioplankton is an important component of marine as well as fresh waters and accounts for a large fraction of the production of particulate matter in those ecosystems. The concept of bacteria as providers of essential nutrients in aquatic food webs has been raised most recently in relation to the utilization of bacteria as a food source in aqua...
Article
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Branchinecta orientalis is a fairy shrimp endemic to the Palearctic region, from Mongolia to Spain. The patchy nature of its habitat is thought to result in a high degree of subdivision among populations, potentially promoting speciation. We combined morphometric characteristics with molecular phylogeny of cytochrome c oxidase I (COI) to test wheth...
Article
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The microbiota in solar salterns plays an important role in salt production quantitatively and qualitatively. Bohai Bay coast is the major sea salt producing area in China. However, few ecological characterization studies of the Bohai Bay salt ponds, particularly of their microbial diversity, have been conducted. This study investigated the structu...
Article
Two Artemia culture experiments were conducted in a zero water exchange system at salinity 80 g L-1. In Experiment I, different carbon sources including molasses, glucose, sucrose and corn flour were given to supplement suboptimal feeding with the microalgae Dunaliella viridis, whereas the control was fed only D. viridis. In Experiment II, molasses...
Article
The brine shrimp Artemia franciscana is an important live feed for fish and shellfish larviculture. Cysts of Artemia are naturally found in a wide variety of harsh environments worldwide where they are exposed to different changing environmental conditions. Artemia cysts are also exposed to different hydration/dehydration (H/D) conditions during th...
Article
The brine shrimp Artemia represents a widespread genus of microcrustaceans adapted to hypersaline environments. The species of this genus have been the subject of numerous phylogenetic studies, but many open questions remain, especially for Eurasian Artemia lineages. Artemia sinica Cai, 1989 and Artemia tibetiana have a restricted geographical dist...
Article
Nauplii hatching from Artemia cysts are crucial in larviculture nutrition. Artemia cysts may be exposed to repeated hydration/dehydration (H/D) cycles pre-harvesting or during processing and storage. To observe the effect of these cycles on cyst quality, Artemia franciscana cysts were exposed to a comprehensive set of various H/D treatments, differ...
Article
Full-text available
Asia harbors a diverse group of sexual and asexual Artemia species, including the invasive Artemia franciscana, which is native to the Americas. The phylogeny of Asian Artemia species and the phylogeography of the introduced A. franciscana from 81 sampling localities in Eurasia, Africa and America were elucidated using mitochondrial (COI) and nucle...
Article
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The nature of genetic divergence between the Artemia population native to San Francisco Bay, (SFB) USA and those from the introductions of SFB material in the Kenyan coast two decades ago were investigated using the mitochondrial DNA (mtDNA) and heat shock protein 70 (Hsp70) gene molecular markers. The DNA was extracted from 80 single Artemia cysts...
Article
The notion that phenotypic traits emerging from environmental experiences are heritable remains under debate. However, the recent report of nonmendelian transgenerational epigenetic inheritance, i.e., the inheritance of traits not determined by the DNA sequence, might make such a phenomenon plausible. In our study, by carrying out common garden exp...
Chapter
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This chapter provides an update on the most common zooplankton live feed species used in hatchery rearing of fish and shellfish larvae, namely rotifers, Artemia and copepods. Each section starts with a summary of the biology and ecology of these species. An overview of the most common techniques to culture, feed, harvest, disinfect and preserve the...
Article
We studied the effect of pH (pH 5, 6, 7 and 8) on the hatching percentage, survival and reproduction of Artemia strains in Bohai Bay salt ponds. Strains included parthenogenetic Artemia from Bohai Bay (BHB), Artemia franciscana from San Francisco Bay, and A. franciscana artificially produced in salt ponds in Vietnam. The latter was included as a po...
Article
This paper studied the effects of increased carbon (C) and nitrogen (N) supplementation in pond production of Artemia franciscana cysts in southern Vietnam. Pig manure and tapioca were used to supplement the natural food of Artemia in the ponds at a fixed C/N ratio 10. Addition of pig manure and tapioca at a standard rate (0.210 kg ww and 0.319 kg...
Data
List of the 65 primer combinations used for AFLP analysis1 E: Eco RI primer with three selective bases; M: Mse I primer with three selective bases (1, 2, 3, 4 correspond to A, C, G, T). (DOC)
Article
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We report on the construction of sex-specific linkage maps, the identification of sex-linked markers and the genome size estimation for the brine shrimp . Overall, from the analysis of 433 AFLP markers segregating in a 112 full-sib family we identified 21 male and 22 female linkage groups (2 = 42), covering 1,041 and 1,313 cM respectively. Fifteen...
Article
Great Salt Lake (GSL), USA is the main source of the commercially important Artemia franciscanaKellogg (1906) cysts used in larviculture. Our objective was to document the presence of parthenogenetic Artemia in GSL analysing a series of non-commercial samples harvested over the period 1997–2005. Laboratory cultures suggested that sex ratios were sk...
Article
The effect of carbon/nitrogen (C/N) ratio manipulation in feed supplements on Artemia production and water quality was investigated in solar salt ponds in the Mekong Delta, Vietnam. It was assumed that development of bacterial bioflocs through C/N manipulation would improve Artemia production and water quality as demonstrated in freshwater and mari...