Ghansham Dixit’s research while affiliated with Shivaji University and other places

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Publications (35)


Figure 1: A: Chromatogram of Standard 6-Gingerol; B: Chromatogram of control; C: Chromatogram of highest 6- Gingerol content in EMS treatments (0.30%); D: Chromatogram of lowest 6-Gingerol content in EMS treatments (0.60%). 
Figure 2. Correlation between biochemical parameters of EMS 8 hrs treatments:- A: Correlation between Flavonoid and Phenolics; B: Correlation between Flavonoid and Antioxidant (FRAP); C: Correlation between Phenolics and Antioxidant (FRAP); D: Correlation between Flavonoid and Antioxidant (DPPH); E: Correlation between Phenolics and Antioxidant (DPPH); F: Correlation between Antioxidant tests: FRAP and DPPH; G: Correlation between 6-gingerol and Flavonoid content; H: Correlation between 6-gingerol and Phenolic content; I: Correlation between 6-gingerol and Antioxidant activity (FRAP); J: Correlation between 6-gingerol and Antioxidant activity (DPPH). 
Figure 3: Correlation between biochemical parameters of EMS 4 hrs treatments:- A: Correlation between Flavonoid and Phenolics; B: Correlation between Flavonoid and Antioxidant (FRAP); C: Correlation between Phenolics and Antioxidant (FRAP); D: Correlation between Flavonoid and Antioxidant (DPPH); E: Correlation between Phenolics and Antioxidant (DPPH); F: Correlation between Antioxidant tests: FRAP and DPPH; G: Correlation between 6-gingerol and Flavonoid content; H: Correlation between 6-gingerol and Phenolic content; I: Correlation between 6-gingerol and Antioxidant activity (FRAP); J: Correlation between 6-gingerol and Antioxidant activity (DPPH). 
Table 4 . ANOVA and Correlation between biochemical parameters of EMS 8 hrs treatments.
Table 5 . ANOVA and Correlation between biochemical parameters of EMS 4 hrs treatments.
RP-HPLC analysis of 6-gingerol and assessment of antioxidant activities in EMS treated ginger
  • Article
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January 2010

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9 Citations

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Ghansham Dixit

Present investigation deals with RP-HPLC analysis of 6-gingerol from rhizome of ginger treated with EMS at different concentrations. The concentration levels were 0.10, 0.15, 0.20 and 0.25% for EMS 8 hour treatment and 0.30, 0.40, 0.50 and 0.60% for 4 hour treatment. The antioxidant activity of extracts were assessed by DPPH radical scavenging method and FRAP. Total phenolic content was determined using the Folin–Ciocalteu (F– C) method. The results showed that the 6-gingerol content increased with an increase in EMS dose initially (0.30%) and decreased at the higher dose (0.60%) in EMS 4 hrs treatments while there was no definite trend observed in EMS 8 hrs treatments. Antioxidant activity and the total phenolic content showed significant correlation with 6-gingerol content.

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Figure 2. Correlation in mutation % between VM1 and VM2 . 
Induction of chlorophyll mutants in Zingiber officinale Roscoe by Gamma Rays and EMS

January 2010

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285 Reads

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8 Citations

The present investigation comprises the data on frequency percentage of chlorophyll mutations in the different doses of gamma rays coupled with EMS in ginger. Different mutation frequencies and width of mutation spectra were induced under the action of different concentrations of the two applied mutagens. The spectrums of chlorophyll mutations (albino, xantha, and chlorina) were observed and grouped. The overall mutation spectrum for ginger showed that xantha occurred with the highest frequency, followed by chlorina and albino. The mutagenic effectiveness decreased with the increase in dose of mutagen for both the mutagen indicating that negative relationship between effectiveness and dose of mutagen. EMS provided more number of chlorophyll mutants than gamma rays in this investigation.


In vitro multiplication and conservation of an endemic and critically endangered plant Aponogeton bruggenii Yadav & Govekar

January 2010

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148 Reads

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2 Citations

National Academy Science Letters

In vitro technique has been used to rescue an endemic and critically endangered plant species Aponogeton bruggenii Yadav & Govekar from Maharashtra (India). Tubers of the plants were successfully cultured on MS medium. Multiple shoot were induced from tuber explants. Excised shoots on transfer to rooting medium produced roots. The plants on transfer to soil were acclimatized and were continued to grow, This approach promises to save this plant from extinction.


Somatic Embryogenesis and Plant Regeneration of a Rare and Multipurpose Medicinal Plant Hemidesmus Indicus (L.) Schult. Plant Cell Biotechnology and Molecular Biology.

January 2008

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138 Reads

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4 Citations

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Hemidesmus indicus (L.) Schult. is an important, multipurpose and a rare medicinal plant. It is locally known as 'Anantmul' and is quite familiar as Indian Sarsaparilla. Protocol for in vitro plant regeneration from callus via somatic embryogenesis has been developed for this plant using leaf explants. Nodular embryogenic callus was initiated from leaf explants when inoculated onto MS medium supplemented with 2,4-D (1 mg/l). These calli when sub-cultured on hormone free N,N-basal medium developed into somatic embryos within 8-weeks in dark condition. Subsequently, embryos germinated into complete plantlets on the same medium with well developed shoot and root system. The in vitro derived plants were hardened in the soil, sand and compost mixture (2:1:1).


Karyomorphological Analysis of Delphinium malabaricum (Huth) Munz: A Rare Endemic Potential Ornamental Plant from Peninsular India

July 2007

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65 Reads

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11 Citations

CYTOLOGIA

Genus Delphinium L. with about 320 species, distributed in North temperate regions of the world is represented by about 24 species in India, mainly found in Himalayan regions. Delphinium malabaricum (Huth) Munz is the only rare endemic species of the genus Delphinium restricted to Northern Western Ghats of peninsular India. It has medium-sized violet–blue–metallic blue spurred flowers of great ornamental value. The present paper describes distribution, morphology, ornamental potential and karyotype of the species. The chromosome number 2n=16 and n=8 is reported for the first time in this species and the karyotype is found to be moderately symmetrical (2b). Its utilization as an ornamental plant after its domestication seems to be better strategy for conservation and utilization of this rare endemic of Northern Western Ghats.


Citations (30)


... Lotus mengandung flavonoid dan tanin dengan aktivitas antioksidan tinggi, senyawa fenol, dan asam galat (18.607%) serta senyawa kimia lainnya seperti tanin, terpenoid, dan polifenol (Kolar et al., 2011). Lotus biasanya hidup di rawa sebagai tanaman air. ...

Reference:

Pelatihan Pemanfaatan Batang Lotus Sebagai Bahan Pangan Fungsional Pada Produk Tonjong Chips (Tonjchips) di Desa Bontolangkasa Selatan Kabupaten Gowa
Induced chlorophyll mutations in Delphinium malabaricum (Huth) Munz.
  • Citing Article
  • June 2011

Journal of Applied Horticulture

... In particular, in vitro techniques are of critical importance for species with small or endangered populations (Gonçalves et al. 2010;Mallón et al. 2010). The transplantation of in vitro raised plantlets into natural habitats is a viable approach for plant conservation and restoration (Chavan et al. 2018;Adsul et al. 2019). These in situ conservation efforts involve the expansion of the area of occupancy of the species (Peixoto et al. 2023). ...

In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad

Journal of Genetic Engineering and Biotechnology

... In ornamental species, mutated selections include changes in flower shape, flower color, and leaf chlorophyll variegation (Datta and Teixeira da Silva 2006). New flower colors have been isolated from gamma-irradiated Catharanthus roseus (Kannabiran et al. 2017), Chrysanthemum (Setia et al. 2020), and Delphinium malabaricum (Kolar et al. 2015). ...

Mutational Changes In Delphinium malabaricum (Huth.) Munz.: A Potential Ornamental Plant

Journal of Horticultural Research

... The proposed system could be useful to standardize the protocol for generation of this endangered medicinal plant species for its ex-situ conservation. Aponogeton bruggenii Yadav & Govekar has been also successively conserved through the standardization of protocol with multiple shoot induction method using tuber as explant(Jagtap et al. in 2010). ...

In vitro multiplication and conservation of an endemic and critically endangered plant Aponogeton bruggenii Yadav & Govekar
  • Citing Article
  • January 2010

National Academy Science Letters

... Several studies revealed the biological activities of 6-gingerol, including antioxidant, anticancer, anti-inflammatory, antimicrobial, antiapoptotic, and many more. The study by Pawar et al. (2016) revealed that there is a strong and significant correlation between 6-gingerol and free radical scavenging activities. Thus, LC-QTOF/MS and correlation analysis (R) were performed to quantify the concentration of 6-gingerols in BG extracts affected by different drying methods and fresh ginger extracts and to assess the interaction between the 6-gingerol concentration and DPPH free radical scavenging activity, respectively. ...

Corrigendum to “RP-HPLC analysis of phenolic antioxidant compound 6-gingerol from different ginger cultivars” [Food Chemistry 126 (2011) 1330–1336]

Food Chemistry

... Consolida ajacis, C. ambigua, D. ambiguum), a popular winter seasonal in gardens, being widely used as ornamental for its attractive spike like raceme. Several efforts have been made to create plants with large-sized blossoms of shades not existing naturally in Delphinium by induction of polyploidy (Singh 1991), hybridization (Royal Horticultural Society 1949;Legro 1961), mutation breeding and tissue culture (Honda and Tsutsui 1997;Honda et al. 2003;Kolar et al. 2015). The cytological studies carried out until date in D. ajacis have been restricted to the study of X-ray induced chromosomal rearrangements (Jain et al. 1963), karyotypes, meiotic behaviour of chromosomes in pollen mother cells (PMCs) (Singh and Roy 1983;Mehra and Remanandan 1972;Subramanian 1985;Kaur and Sidhu 2014) and study of chromosomes in endosperm (Mandal and Basu 1978) and colchitetraploids (Singh 1991). ...

RP-HPLC Analysis of an Alkaloid – Mythyllycaconitine from Mutagenic Delphinium Malabaricum (Huth) Munz.

... In comparison to aspirin, this plant is also used to enhance appetite and cure asthma, and it is thought to be a possible anti-platelet agent. It has anti-inflammatory and analgesic qualities similar to non-steroid anti-inflammatory medications, but without the gastrointestinal bleeding and ulcer development adverse effects (Pawar et al. 2015). The rhizome extract of Zingiber officinale is utilized here as a reducing and stabilizing agent. ...

RP-HPLC analysis of phenolic antioxidant compound 6-gingerol from in vitro cultures of Zingiber officinale Roscoe
  • Citing Article
  • January 2015

Plant Science Today

... A Rubia cordifolia hairy root culture was treated with various concentrations of chitosan (50 mg l −1 to 200 mg l −1 ) for alizarin production. Hairy roots treated with 150 mg l −1 chitosan accumulated tenfold (4.65 ppm) higher amounts of alizarin than the normal hairy roots (0.48 ppm) (Kudale et al. 2014). Existing reports revealed that the Agrobacterium strains, explant, co-culturing method, light, sucrose, and elicitor concentrations undoubtedly play vital roles in anthraquinone production under in vitro conditions. ...

Effect of Chitosan Elicitation on Alizarin Production in Hairy Root Cultures of Rubia cordifolia L.

Journal of Chitin and Chitosan Science

... Different in vitro culture systems support the enhanced production of secondary metabolites in numerous plants (Kshirsagar et al. 2015;Dey et al. 2019;Shahnawaz et al. 2021;Pandey et al. 2022;Manokari et al. 2023). The first attempt at the extraction of industrially important phytoconstituents from Z. zerumbet was based on in vitro micro-rhizome development, especially for the production of zerumbone by Idris et al. (2007). ...

Highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Swertia lawii Burkill

Biotechnology Reports

... Chemical mutagenesis has been extensively recognized as an effective approach for inducing a broad spectrum of morphological and genetic variations in plant species Kolar et al. 2013;Jahan et al. 2020;Shaikh et al. 2021;Singh et al. 2021). Compared to physical mutagens, chemical agents generally yield a higher frequency of mutations, offering increased effectiveness and more specific targeting of genetic alterations (Kharkwal 1999;Bhat et al. 2005). ...

EMS, sodium azide and gamma rays induced meiotic anomalies in Delphinium malabaricum (Huth) Munz

Israel Journal of Plant Sciences