Geysiane Moreira Gerotti’s research while affiliated with Universidade Paranaense and other places

The present study evaluated the efficiency of a protocol for micropropagation of stem apexes and nodal segments of basil ‘Grecco a Palla’ in various concentrations of 6-benzylaminopurine (BAP) and 3-indole butyric acid (IBA). A completely randomized design was used with six treatments distributed in five replications. A medium without growth regulators favored the survival of Ocimumbasilicum stem apexes inoculated in vitro, and thereby promoted the sprouting of explants, whereas, for nodal segments, it was necessary to use regulators, and the concentration of 0.5 mg.L−1 BAP 0.0 mg.L−1 of IBA was more beneficial for the species.

In vitro, shoot apices cultures provide an alternative means for producing secondary metabolites of medicinal and pharmaceutical importance. In this study, the major objective was to investigate the effect of Copper (Cu) and Methyl Jasmonate elicitors on basil 'Grecco a Palla' shoot apex growth, on the volatile chemical composition, and biological and enzymatic activities. Stem apex, from seedlings grown in vitro, were used as explant for plant propagation. Murashige—Skoog medium, supplemented with five elicitors concentrations (T1: 0 µM, 0 µM; T2: 25 µM, 0 µM; T3: 0 µM, 5 µM, T4: 25 µM, 5 µM; T5: 75 µM, 5 µM de Cu and MeJa, respectively) was used for the inoculation. Cooper in combination with MeJa (T3 and T4) reduced the occurrence of oxidation (20%) and hyperhydricity (77%) in the shoot when compared to the control treatment. 25 µM Cu and 5 µM MeJa increased the antioxidant activity by 72.5% (DPPH), the enzymatic activity of Phenylalanine ammonia-lyase (PAL) by 95%, the total phenolic compounds by 33,33%, and superoxide dismutase (SOD) enzymatic activity by 37%. In the 75 µM Cu and 5 µM MeJa treatment resulted in an increase of 20% in the ascorbate peroxidase enzyme activity. Those treatments promoted a higher number of volatile compounds to T4 (20) and T5 (28), of which α-bergamotene, methyl eugenol, and linalool were found to be the majority. β-Pinene, Myrcene, γ-Cadinene, and δ-Cadinene were only identified in T5. Therefore, the above-mentioned elicitors might be used in combination with the described concentrations, in order to improve the compound synthesis and antioxidant activity.

A Salmonelose é uma doença de grande impacto na saúde única, ou seja, saúde humana, animal e ambiental. Por ser uma zoonose, é necessário controlar a sua disseminação devido a sua associação aos casos de Doenças transmitidas por Alimentos (DTA’s). O Brasil é o maior exportador da carne de frango no mundo, sendo assim, a identificação da presença de carne contaminada com Salmonella spp. pode trazer grande prejuízos à agroindústria. Sendo assim, o objetivo deste trabalho foi realizar um levantamento de artigos publicados na base de dados da Scielo relacionados ao controle de Salmonella spp. em frangos de corte. Como estratégia de pesquisa dos dados, utilizou-se a seguinte frase no item palavras-chave: Controle de Salmonella em frangos de corte. No total, 14 artigos oriundos do Brasil foram encontrados. No entanto, apenas 12 artigos foram analisados, visto que dois artigos não abordavam tratamentos contra a Salmonela spp. Verificaram-se resultados positivos para o controle de Salmonella spp. com probióticos, ácidos orgânicos, flagelina recombinante, óleos essenciais e prebióticos, no entanto, variações nos resultados podem estar relacionados com o produto utilizado, tempo de tratamento e cepa bacteriana utilizada no desafio.

Milk and its derivatives are highly consumed foods worldwide, with recognized nutritional importance. The search for the production of products with superior quality is constant. For the present work, 26 milk-producing properties were selected, with a total of 506 milk samples collected during the period from October 2019 to May 2020 being evaluated. The objective of this study was to evaluate the quality of milk produced in dairy properties in the region west Paraná, classified as good or bad based on the results of the Somatic Cell Count (SCC) and through sampling (n = 10) to evaluate the resistance profile of enterobacteria and Staphylococcus spp. isolated from milk samples, in addition to the presence of the mecA gene in strains of Staphylococcus spp. resistant to oxacillin. There were significant differences between the good and bad properties for the levels of lactose, SCC (cell/mL), and Standard Plate Count (SPC) (CFU/mL). The strains of Staphylococcus spp. showed differences in the percentage of resistance in relation to the good and bad properties for antibiotics: tetracycline, ciprofloxacin, oxacillin, amikacin, clindamycin, gentamycin, and erythromycin. The mecA gene was not detected in any of the coagulase-negative Staphylococcus isolates that showed resistance to oxacillin. For enterobacteria, the isolated species differed in relation to the classification of properties, with predominance for Escherichia coli (40%) for properties classified as bad and Hafnia alvei (40%) for those classified as good. The percentage of antibiotic resistance compared to enterobacteria isolates was higher in properties classified as good. Monitoring through microbial culture and antibiogram is extremely important, favoring the correct choice for the treatment of animals with a reduced selection of resistant strains.

The in vitro development of a plant is controlled by factors that promote a series of plant responses, which interfere with tissue organogenesis and morphology. For plants of the family Lamiaceae, these factors remain unknown or poorly understood, hindering in vitro cultivation of these plants. The basil cultivar ‘Grecco a palla’ has attractive chemical properties for medicinal, pharmaceutical, and cosmetic industries; however, its production is limited due to the lack of appropriate cultivation conditions. Two types of explants of this species (nodal segments and stem apexes) were grown in culture media with auxin and cytokinin, and their development was followed for 60 days. During in vitro cultivation, both explants were subjected to higher concentrations of plant growth regulators (PGRs) produced only calluses, without induction of shoots. Small amounts of regulators favored hyperhydricity as nodal segments or stem apexes in the absence of PGRs produced plants with disturbances, including brittle, light green, and thick leaves. In this case, there was an increase in the cell layers of palisade parenchyma, which had large cell spaces and larger cells. This tissue also advanced to spongy parenchyma and compressed it. The stomatal density was low; however, the stomata were larger with additions mainly in the guard cells and the stomatic opening. Therefore, stem apexes in the absence of PGRs produced more vigorous plants, whereas nodal segments with low amounts of cytokinins and auxins developed a well-branched and abundant root system.