Gareth Fuller’s research while affiliated with Arizona State University and other places

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Publications (1)

Spectroscopic characterization of gold nanobipyramids (AuNBPs). (a) A representative absorption spectrum of the AuNBP seed solution. (b) Normalized AuNBP absorption spectra as a function of seed volume used in a 4 mL reaction (with 8-HQL as a reducing agent). (c) Normalized absorption spectra of AuNBPs for ascorbic acid or 8-HQL as reducing agents. (d) TEM image of high-purity AuNBPs indicates a uniform size distribution. A representative image from n = 3 independent TEM experiments is shown. Additional TEM images are included in Supplementary Figure S1.
Characterization of AuNBP-LASE films. (a) Representative digital photograph of an AuNBP-LASE film prepared by solvent evaporation. (b) AuNBP absorption spectra normalized with maximum absorption in each spectrum as a function of hydration with saline (average of n = 3). (c) A representative absorption spectrum of dry AuNR-LASE. (d) photothermal (temperature) response of AuNBP-LASE and e) AuNR-LASE irradiated with an 808 nm laser at different power densities. The laser was irradiated (“on”) for 30 s and turned off (“off”) for 30 s for three consecutive cycles. Representative data from n = 3 independent experiments. (f) Dynamic mechanical analysis (DMA) of AuNBP-LASE (Average of n = 3).
Skin sealing efficacy with AuNBP-LASE. (a) Digital photograph of an AuNBP-LASE used in laser skin sealing (left). Murine skin incisions sealed with sutures and AuNBP-LASE are shown on the right as indicated. (b) A cartoon showing vapometer and cutometer measurement sites in mice whose incisional wounds were laser-sealed with AuNBP-LASE. (c) Transepidermal water loss (TEWL) measurements of skin incisions sealed with sutures and AuNBP-LASE on day 2 post wounding d) Representative cutometer curves of the healing skin on day 2 post wounding using Mode 1 of the cutometer. (e) Comparison of dimensionless parameters obtained from cutometer measurements for unwounded skin, suture-approximated skin, and AuNBP-LASE-sealed skin. Descriptions of individual parameters are provided in Table 1. Representative images and data are shown from at least n = 3 independent experiments.
Histological analyses of mice incisions closed with sutures and AuNBP-LASE on day 2 post wounding. Representatives of n = 3 independent images are shown here; other images are shown in Supplementary Figure S4. (Top) Hematoxylin and eosin (H&E) staining (red lines indicate the dermal gap, black arrows indicate the epidermal edge, blue box shows the area of glue integration with the tissue, and green box illustrates the effect of laser-induced hyperthermia on dermal collagen) and (bottom) Masson’s trichrome staining. (Right) Quantification of epidermal gap for incisions closed using sutures and AuNBP-LASE.
Gold nanobipyramids-based laser-activated sealants for effective skin sealing and repair
  • Article
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February 2024

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64 Reads

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1 Citation

Shubham Pallod

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Gareth Fuller

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Trishita Chowdhury

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Kaushal Rege

Anisotropic gold nanostructures have gained increased attention for biomedical applications because of their remarkable optical properties. An emerging type of gold nanostructure—gold nanobipyramids (AuNBP)—has been shown to exhibit superior absorption properties compared to conventionally used gold nanoparticles, which makes them attractive for photothermal applications. We generated a high-shape-purity dispersion of AuNBP using a seed-mediated method and embedded them as photothermal conversion agents in a silk fibroin matrix to investigate their efficacy in photothermal sealing of incisional wounds in immunocompetent mice. These AuNBP-doped laser-activated sealants, or AuNBP-LASE were able to absorb near-infrared laser energy and convert it to heat, thereby inducing transient hyperthermia in the wound and the surrounding tissue. This photothermal conversion facilitated rapid sealing of the skin tissue by the AuNBP-LASE, which resulted in faster functional recovery of skin barrier function compared to nylon sutures at the early stages of repair. Further, the biomechanical properties of the healing skin closed with AuNBP-LASE those of intact skin more rapidly compared to incisions approximated with sutures. Histology studies indicated higher penetration of the LASE within the volume of the incision in skin tissue, lower scab formation, and a similar epidermal gap compared to conventional suturing. These results demonstrate that AuNBP-LASEs can be effective as wound approximation devices for photothermal sealing.

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