Gabriel Rossato’s research while affiliated with University of the West of Santa Catarina and other places

A composição químico-bromatológica da silagem de milho produzida por produtores de Faxinal dos Guedes é parcialmente satisfatória. Além disso, este trabalho elucubra a hipótese de que a fertilidade do solo onde a silagem é feita pode ser limitada.

Introduction: Alternanthera brasiliana, commonly known as Penicillin, belongs to the Amaranthaceae family and is popularly used in the treatment of various pathologies due to its anti-inflammatory, antibacterial and analgesic properties and its inhibitory activity against the herpes simplex virus. Despite its empirical use, pharmaceutical industry investigations into the biological characteristics of this species have been limited. Objective: Carry out a systematic literature review on the biological activities, types of extraction and chemical composition of Alternanthera brasiliana, aiming to provide an update on the state of the art of knowledge regarding the species, contributing to the scientific prospection of new drugs. Contribute to the development of drugs and expand understanding of the pharmacological properties of Alternanthera brasiliana. Materials and methods: Therefore, the guiding question of this research was the following: Do the chemical components and biological properties identified in Alternanthera brasiliana have pharmacological actions? The adopted methodology comprises a systematic literature review, searching for articles in the PubMed, SciELO, Web of Science, EBSCO, SCOPUS and Science Direct databases, covering the period from 2003 to 2023. Studies that address the chemical composition and the biological properties of Alternanthera brasiliana, using analytical methods such as LC-MS and GC-MS. Results: The main constituents identified were phenolic compounds, flavonoids, tannins, glycosides, saponins, alkaloids and terpenes. These compounds were mainly extracted from leaves using hydroalcoholic, aqueous, ethanolic and methanolic extracts. Its highlighted biological activities include anti-inflammatory, analgesic, antioxidant, wound healing and antimicrobial effects. Conclusion: Alternanthera brasiliana shows promise as a source for the development of new medicines due to its chemical composition and biological properties. Originality: This study offers perspectives for application in the development of pharmaceuticals and nutraceutical products.

Microencapsulation is an alternative to increase the survival capacity of microorganisms, including Yarrowia lipolytica, a widely studied yeast that produces high-value metabolites, such as lipids, aromatic compounds, biomass, lipases, and organic acids. Thus, the present study sought to investigate the effectiveness of different wall materials and the influence of the addition of salts on the microencapsulation of Y. lipolytica, evaluating yield, relationship with cell stability, ability to survive during storage, and in vitro application of ruminant diets. The spray drying process was performed via atomization, testing 11 different compositions using maltodextrin (MD), modified starch (MS) and whey protein concentrate (WPC), Y. lipolytica (Y. lipo) cells, tripolyphosphate (TPP), and sodium erythorbate (SE). The data show a reduction in the water activity value in all treatments. The highest encapsulation yield was found in treatments using MD + TPP + Y. lipo (84.0%) and WPC + TPP + Y. lipo (81.6%). Microencapsulated particles showed a survival rate ranging from 71.61 to 99.83% after 24 h. The treatments WPC + Y. lipo, WPC + SE + Y. lipo, WPC + TPP + Y. lipo, and MD + SE + Y. lipo remained stable for up to 105 days under storage conditions. The treatment WPC + SE + Y. lipo (microencapsulated yeast) was applied in the diet of ruminants due to the greater stability of cell survival. The comparison between the WPC + SE + Y. lipo treatment, wall materials, and the non-microencapsulated yeast showed that the microencapsulated yeast obtained a higher soluble fraction, degradability potential, and release of nutrients.

Nosso objetivo foi determinar se a suplementação de microalgas (Schizochytrium limacinum) afeta a produção diária, composição e perfil de ácidos graxos do leite orgânico. Oito vacas em lactação foram mantidas a pasto e divididas em dois grupos: as alimentadas com sabugo de milho como suplemento duas vezes ao dia durante a ordenha (CTL) e as alimentadas com sabugo de milho misturado com 100 g de microalgas (ALG) por vaca diariamente. As microalgas não afetaram a produção e composição diária do leite, mas foi observada uma tendência de redução da gordura do leite. O nível de ácido esteárico no leite de vacas alimentadas com ALG foi significativamente reduzido em 2,46 vezes, enquanto os níveis de ácido elaídico e ácido linoléico conjugado foram significativamente elevados em 3,3 vezes e 1,8 vezes, respectivamente. Uma proporção significativamente maior de PUFA: MUFA foi observada no tratamento com ALG, enquanto a proporção de MUFA: ácido graxo saturado mostrou uma tendência a aumentar (P = 0,073). Microalgas ricas em ácidos graxos ômega-3 enriquecem com sucesso o leite orgânico sem afetar negativamente a produtividade ou composição. Os consumidores podem ser atraídos para aumentar a ingestão de gordura polinsaturada ômega 3 do leite orgânico. Esses resultados podem apoiar a decisão de nutricionistas e agricultores de alimentar o gado leiteiro com microalgas, uma vez que é economicamente viável.