G. Radaelli's research while affiliated with University of Padova and other places

Publications (62)

Article
Full-text available
Background: The dietary supplementation of yeast cell wall extracts (YCW) has been found to reduce pathogenic bacteria load, promote immunoglobulin production, prevent diseases by pro-inflammatory responses, and alter gut microbiota composition. This study evaluated growth and slaughter results, health, gut morphology, immune status and gut transc...
Article
Environmental temperature is one of the critical factors affecting fish development. The aim of this study was to examine the impact of three different rearing temperatures (16°C, 19°C and 22°C) throughout the endogenous feeding phase of the Siberian sturgeon Acipenser baerii. This was performed by assessing i) larval survival and growth, ii) immun...
Article
Full-text available
During recent years, research on meat quality in poultry has aimed to evaluate the presence and consequences of breast myopathies as well as the factors which can affect their occurrence by modifying the growth rate. A total of 900 broiler chickens were reared until slaughter (48 D) to evaluate the effect of 2 genetic lines (A vs. B) and feeding pl...
Conference Paper
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Teaching4Learning@UNIPD started in 2016, and it representes the first step for the University of Padua to foster innovative teaching in response to European recommendations. It encourages faculty to experiment with new teaching strategies; involves students and promotes their active participation in educational activities; de-privatizes teaching; a...
Article
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To evaluate muscle fiber degeneration (MFD) associated with white striping and wooden breast, pectoralis major of 192 broilers differing for genotype (standard vs. high breast yield), gender, and feeding regime (ad libitum vs. restricted rate 80% from 13 to 21 d of age) were sampled at 14, 21, 28, 35, and 46 d of age for histological analyses by he...
Article
Full-text available
Due to their importance for the control of meat quality in broiler chickens, the present study aimed at identifying the factors associated with the occurrence of myopathies and characterizing the meat properties when affected by myopathies. To this aim, a total of 768 broiler chickens were reared until slaughter (46 d) to evaluate the effect of gen...
Article
A total of 282 crossbred rabbits of both genders were divided into six experimental groups and fed ad libitum six diets formulated in a 2×3 factorial arrangement based on three dietary energy levels (low, approximately 8.45 MJ/kg; medium, 9.9 MJ/kg: and high, 11.5 MJ/kg) and two crude protein levels (15% and 17%). The increase of dietary energy was...
Article
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The objective of this work was to investigate the expression of heat shock protein 70 (HSP70) by Western blot (WB) in swine liver. Subsequently, the study aimed to apply this method to two experimental groups of heavy pigs raised in different confinement systems: intensive/indoor (Group A) and extensive/outdoor (Group B). Thirty-six crossbred comme...
Article
From 29 d of age until slaughter (78 d), 246 hybrid rabbits were divided into six experimental groups and fed six diets ad libitum according to a bi-factorial arrangement with two soluble fibre-to-starch ratios (0.5 and 1.1), obtained by replacing starch (from 178 to 130 g/kg) with soluble fibre (from 84 to 141 g/kg), and three levels of crude prot...
Article
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The aim of the present work was to evaluate the expression of 8-OHdG (8-hydroxydeoxyguanosine) in the benthic fish Zosterisessor ophiocephalus collected in two differently polluted sites of the Venetian lagoon (Porto Marghera and Caroman). We compared our data on 8-OHdG with those of CYP1A (Cytochrome P450, family 1, subfamily A, polypeptide 1), wh...
Chapter
The temperate aquatic environment is affected by two primary seasonal components, temperature and photoperiod. Many organisms respond to seasonal change physiologically, behaviorally or both. The aim of this study was to investigate the effect of seasonality on cortisol, hematological, and innate immune parameters in European sea bass (Dicentrarchu...
Article
The enigmatic rodlet cells (RCs) are characterized by conspicuous inclusions named "rodlets". They were discovered over 100 years ago and were considered as parasites but shortly afterward interpreted as endogenous cells. The RCs have been described in different tissues of marine and freshwater teleosts, but their origin and function remain unknown...
Article
In the last decades there have been several evidences that traditionally used live preys like rotifers and Artemia salina have nutritional deficiencies that result in a general decrease of fish health, causing anomalies in the development, in growth and in pigmentation. In this study a partial of total replacement of traditional live preys with pre...
Poster
Monothematic conference on the endocannabinoid system “Endocannabinoids: From biology to clinics”
Article
The temperate aquatic environment is affected by two primary components of season, temperature and photoperiod, during the annual cycle. Many organisms respond to seasonal change physiologically, behaviorally or both. The aim of this study was to investigate the effect of seasonality on cortisol, hematological and innate immune parameters in Europe...
Article
From weaning (34 d of age) until slaughter (76 d), 216 hybrid rabbits were divided into six experimental groups and fed ad libitum six iso-protein (CP: 15.9% as-fed), iso-starch (9.1%) and iso-ADF (21.4%) diets formulated in a bi-factorial arrangement, with three soluble fibre levels (LS: 5.8% vs. MS: 7.4% vs. HS: 8.5%) and two protein sources (Soy...
Article
Full-text available
From weaning (34 d of age) until slaughter (76 d) 216 hybrid rabbits were divided into six experimental groups and fed ad hbitum six iso-protein (CP 15 9% as fed) iso starch (9 1%) and iso AIDE (21 4%) diets formulated in a bi factorial arrangement with three soluble fibre levels (LS 5 8% vs MS 7 4% vs HS 8 5%) and two protein sources (soy soybean...
Article
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The cellular localization of IGF-I, IGF-II and MSTN proteins was investigated during ontogenesis of triploid sea bass (Dicentrarchus labrax) by an immunohistochemical approach. The results were compared with those observed in diploids. IGF-I immunostaining was mainly observed in skin, skeletal muscle, intestine and gills of both diploids and triplo...
Article
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To evaluate the effect of digestible fibre (DF, hemicelluloses+pectins)/ADF ratio (1.0 and 1.3) and starch level (12, 15, and 18%) on health status, digestive physiology, growth performance, and carcass traits, 246 rabbits weaned at 27 d were fed until slaughter (76 d) with six diets formulated according to a bifactorial arrangement (2 DF/ADF ratio...
Article
The cellular localization of IGF-I, IGF-II and MSTN proteins was investigated during ontogenesis of triploid sea bass (Dicentrarchus labrax) by an immunohistochemical approach. The results were compared with those observed in diploids. IGF-I immunostaining was mainly observed in skin, skeletal muscle, intestine and gills of both diploids and triplo...
Article
Full-text available
The UE restrictions imposed on the antibiotic utilization in animal husbandry have increased the interest on alternative additives capable of improving animal digestive health. Among the numerous tested products, short chain fatty acids stimulated intestinal mucus production at different level and intestinal cells proliferation in rats (Meslin et a...
Article
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In the present work we investigated by immunohistochemistry the cellular localization of constitutive as well as inducible heat shock protein 70 in several tissues of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) exposed to transport stress.In carp, the constitutive form (HSC70) was detected only in red skeletal muscle of bo...
Article
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In aquaculture, fish are exposed to stressful conditions, which cause an increased synthesis of heat shock proteins (HSPs) at the cellular level. In this work we considered the expression of the constitutive and inducible forms of HSP70 as an indicator of stress caused by transport, during development of the sea bass (Dicentrarchus labrax), a teleo...
Article
In general little is known about hormones and the ontogeny of the stress response in the early developmental stages of chondrostean fishes and in particular of white sturgeon (Acipenser transmontanus, Richardson 1836). In this study, we measured for the first time cortisol and sex steroids (testosterone and estradiol) in eggs, larvae, post-larvae,...
Article
This work describes the cloning of follistatin (FST) cDNA from the gilthead sea bream Sparus aurata (saFST), the expression of FST gene during development and in various tissues including gonads. In addition, recombinant sea bream FST was expressed and purified from medium of CHO-K1 cells.
Article
The cellular localization of IGF-II protein was investigated during larval and postlarval developmental stages of sea bass (Dicentrarchus labrax) by immunohistochemistry using antisera raised against Sparus aurata IGF-II. At hatching, IGF-II immunoreactivity was already present in the skin, developing intestine and skeletal muscle. During larval li...
Article
Stress indicators (plasma cortisol, glucose and lactate) were evaluated in sea bass Dicentrarchus labrax submitted to different pre-slaughter conditions, and cortisol levels in plasma were found to correlate with those detected in mucus, gut contents and muscle. The results demonstrate new possibilities for monitoring stress status in fishes in whi...
Article
The promoter region ( approximately 1400 bp) of myosin light chain 2 gene of fast skeletal muscle from the marine fish Sparus aurata was cloned, sequenced and characterized. It contains a consensus sequence for TATA box, six perfect E-boxes known as binding sites to myogenic basic helix-loop-helix transcription factors and four putative MEF2-bindin...
Article
Morphological, histochemical and immunohistochemical data are presented to demonstrate that the enteric nervous system of the sturgeon is in part composed and arranged differently from other fish. It is composed of neurons which distribute both to the tunica propria-submucosa and tunica muscularis. Nerve cell bodies are small and nerve terminals ru...
Article
The arrangement and innervation of lateral muscle and adductor mandibulae (AM) complex were examined histochemically and immunohistochemically in the sturgeon Acipenser transmontanus. Lateral muscle was arranged in myotomes in which the superficial slow-red muscle layer and the deep fast-white muscle layer were separated by an intermediate layer of...
Article
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Although there are mammalian myoblast cell lines, no fish myoblast cell line has been developed so far. The aim of this study was to develop a culture system of muscle explants for fish, as explants provide an approximation of the in vivo conditions for cell proliferation and differentiation, and enable a close comparison with events in muscle rege...
Article
An eight-week experiment was carried out to evaluate the effects of different protein sources (fish and haemoglobin meal, soybean meal and torula yeast), in practical diets, on growth, body composition and gut morphology of fingerling grey mullet (Mugil cephalus). Weight gain (%), SGR, FCR, N retention, PER, PGR, FDR and carcass composition of fish...
Article
At hatching the gut of sole Solea solea was a primitive epithelial tube which showed vigorous cellular proliferation but only initial regionalization, and no secretory cells. By contrast, at this stage lateral muscle already consisted of a clearly distinct superficial monolayer of parvalbumin-negative fibres, and a deeper layer of parvalbumin-posit...
Article
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The small and large intestine of adult horses were histochemically and immunohistochemically investigated in order to evidence components of the intramural nervous system. The general structural organization of the intramural nervous system was examined by using Nissl-thionin staining as well as the anti-neurofilament 200 (NF200) immunoreaction, wh...
Article
Full-text available
The spatial localization of IGF-II protein and mRNA was investigated during larval and postlarval developmental stages of the gilthead sea bream (Sparus aurata) by immunohistochemistry and in situ hybridization, using specific antisera and riboprobes. Steady-state levels of IGF-II mRNA in larvae were determined by Northern blot analysis and were fo...
Article
The spatial localization of IGF-II protein and mRNA was investigated during larval and postlarval developmental stages of the gilthead sea bream (Sparus aurata) by immunohistochemistry and in situ hybridization, using specific antisera and riboprobes. Steady-state levels of IGF-II mRNA in larvae were determined by Northern blot analysis and were fo...
Article
In this study, the distribution of IGF-I, IGF type I receptor (IGF-IR), and IGF-binding protein 2 (IGFBP-2) was investigated during larval and post-larval developmental stages of the shi drum (Umbrina cirrosa) by immunohistochemistry using antisera raised against Sparus aurata IGF-I and IGF-IR, and against mouse IGFBP-2. Immunoreactivity of the mit...
Article
The occurrence of runt sturgeons is not an unusual event in rearing Acipenser transmontanus. The aim of this study was to describe morphological and histochemical features of the alimentary canal in both feeding and runt fish in order to identify specific structural changes that characterize the differences resulting from the nutritional status of...
Article
We report the characterisation of the gene coding for myostatin (or growth differentiation factor 8, GDF8) in the shi drum, Umbrina cirrosa. Because of its relevant role in muscle growth regulation, GDF8 is an important candidate locus for improvement of animal production.RT-PCR and RACE protocols were used to obtain the full-length GDF8 cDNA seque...
Article
Histochemical staining techniques, which differentiate the main categories of carbohydrates, were applied to sections from different segments of the alimentary canal of the shi drum Umbrina cirrosa L. to study patterns of distribution of epithelial glycoconjugates. In the oesophagus, mucous cells contained sulphomucins, together with a small amount...
Article
Full-text available
The gut of Pantex, a sparid hybrid fish (Pagrus major x Dentex dentex) with a great potential importance for the Italian aquaculture, was histochemically and immunohistochemically investigated in order to evidence components of the intramural nervous and diffuse endocrine systems. The general structural aspects of the intramural nervous system were...
Article
Electron-microscopic examinations of the sturgeon gut were performed. Oesophageal goblet cells were abundant in the stratified epithelium. The ultrastructural features of the secretory granules of the oesophageal and intestinal goblet cells were quite similar to those of other vertebrates. Lobules of multilocular adipose tissue were observed in the...
Article
The gut of silver eels (Anguilla anguilla L.) was investigated in order to describe both the cholinergic and adrenergic intramural innervations, and the localization of possible accessory neuromediators. Histochemical reactions for the demonstration of nicotinamide adenine dinucleotide phosphate, reduced form-(NADPH-)diaphorase and acetylcholineste...
Article
The gut of adult sturgeon was examined. The oesophageal mucosa contained numerous caliciform cells, synthesizing both neutral and acidic glycoconjugates, the latter of the sialylated type. The deep tunica propria-submucosa contained lobules of multilocular adipose tissue, specially abundant during the cold season. The oesophageal tunica muscularis...
Article
Growth of laterarl muscle in the teleost fish Sparus aurata (L.) was examined from hatching to juvenile by a basic morphofunctional approach that takes into account structural and ecophysiological aspects and combines in vivo observations and LM and TEM microscopic analysis. As shown in most teleost fishes, muscle growth proceeds by a double mechan...
Article
The presence of putative neuromodulators in the nerve fibres was investigated in white skeletal muscle of two teleost fish not taxonomically correlated and showing different patterns of innervation (multiple versus focal innervation). Cryostat sections of epaxial, hypaxial and adductor mandibulae (AM) muscles of Sparus aurata and Anguilla anguilla...
Article
Regeneration of skeletal muscle was studied in the sea bream Sparus aurata, in which extensive post-larval muscle hyperplasia contributes to its large adult size, and in the zebrafish Brachydanio rerio, which shows little post-larval hyperplasia and reaches only a small adult size. Small mechanical lesions of body wall muscle were made under genera...

Citations

... This latter result could explain the increase of CD45þ cells we observed in chickens fed the diet supplemented with 0.20% GSE compared to those fed the control diet and the diet with 0.40% GSE, which however was also associated to a decrease in villi height under our conditions. Finally, when age increased, the higher number of goblet and CD3þ cells we recorded was supposed to be associated, respectively, to a defence mechanism and to a triggered local gut immune response, consistently with previous results (R€ ohe et al. 2017;Kogut et al. 2018;Pascual et al. 2020a). ...
... The optimum growth status for Siberian sturgeon has been reported to be about 20 • C (Aidos et al., 2020), but the living temperature raising to 24 and 28 • C caused distinct stress response and thus effected the survival rate during sturgeon farming (Castellano et al., 2017;Aidos et al., 2020;Yang et al., 2021). After 2 weeks acclimatization under laboratory condition, 90 fishes were randomly divided into three groups, each group includes three tanks, 10 fishes per tank. ...
... Regarding to qualitative FR, Taylor, Sakkas, and Kyriazakis (2021) demonstrated that modern broiler genotypes are able to cope with low-density diets by increasing FI when pellets are used. Early FR in broiler chickens was firstly studied by Plavnik and Hurwitz (1985), who documented that it induced compensatory growth during the re-alimentation period (Tůmová et al. , 2022a(Tůmová et al. , 2022b, improved feed efficiency (Gratta et al. 2019), stimulated efficiency of feed utilisation (Metzler-Zebeli et al. 2019) and reduced mortality rate (Mohammadalipour et al. 2017). In Pekin ducks, FR had no negative effect on growth performance but reduced gait abnormalities (Bentley et al. 2020) and improved economic efficiency (Kokoszyński et al. 2019). ...
... In other teleost species, several studies are available on the effect of temperature on osmoregulatory mechanisms Vargas-Chacoff et al., 2009;Radaelli et al., 2010;. Na + /K + -ATPase activity can depend on acclimation temperature, but the effect of temperature depends on the range of temperatures that have been tested and the species considered Zydlewski and Zydlewski, 2012). ...
... Increased yield of carcass and parts is one of the main objectives of the poultry industry because this factor is responsible for higher profitability of the activity (Nortey et al., 2019). The difference in protein deposition at different ages of broilers is mainly due to changes in metabolism, because the difference between anabolic and catabolic processes decreases with advancing age (Radaelli et al., 2017). When the animal reaches its peak growth, the tendency is for fat deposition to increase and protein deposition to decrease. ...
... The effects of seasonality during April and August on the mackerel were observed for blood parameters such as MCH, WBC count, total protein, globulin, glucose, and triglycerides. Seasonal variation in hematological parameters has been found in tench (Tinca tinca), sea bass (Dicentrarchus labrax), Indian carp, spirlin, pejerrey (Odontesthes bonariensis), snow trout (Schizothorax plagiostomusto), and Indian hill (Collazos et al. 1998, Pascoli et al. 2011, Pradhan et al. 2012, Kohanestani et al. 2013, Vigliano et al. 2014, Sheikh & Ahmed 2016, Sharma et al. 2017. Variations in hematologic parameters are due to seasonal water temperature and dissolved oxygen variations (Kohanestani et al. 2013). ...
... a Falcão e Cunha and Lebas (1986), Gidenne (1987Gidenne ( , 1992, Fraga et al. (1991), Gidenne et al. (1991Gidenne et al. ( , 1998Gidenne et al. ( , 2002Gidenne et al. ( , 2004a, García et al. (1992García et al. ( , 1993García et al. ( , 1999García et al. ( , 2002b, Gidenne and Perez (1993, Perez (1994), Bellier and Gidenne (1996), Gidenne and Jehl (1996), Motta-Ferreira et al. (1996), Carabaño et al. (1997), Falcão e Cunha et al. (1998,2000,2004), Gidenne and Bellier (2000), Nicodemus et al. (2002), Xiccato et al. (2006, 2008), Volek et al. (2005, Carraro et al. (2007), Tazzoli et al. (2009Tazzoli et al. ( , 2013Tazzoli et al. ( , 2015, Trocino et al. (2010Trocino et al. ( , 2011Trocino et al. ( , 2013b, Castillo b n = number of diets. c values corrected for intestinal mucins (Fig. 5.4c). ...
Reference: Fiber Digestion
... Males were already significantly heavier (+17.99%) on fattening day 31 compared to females. This corresponds to Trocino et al. [6], who observed significant differences in the live weight between male and female broilers on day 22 and day 46, using a standard-breastyield genotype and a high breast-yield genotype for their investigations. In accordance with these results, Müller Fernandes et al. [11] also discovered a high impact of the sex on live weight for broilers of the strain Ross 308 and other commercial strains. ...
... Besides, it is expressed in theca and granulosa cells and previtellogenic oocytes of the tilapia ovary (Berishvili et al., 2006;Schmid et al., 1999). On the other hand, the igf2 was expressed in theca and granulosa cells of ovaries in tilapia and sea bream (Sparus aurata) (Radaelli et al., 2003;Schmid et al., 1999). ...