G. Paprocka’s research while affiliated with National Veterinary Research Institute and other places

... The serotype O, A, and C viruses have had the widest distribution and have been responsible for outbreaks in Europe, America, Asia, and Africa. The FMDV particle is roughly spherical in shape, and, about 25-30 nm in diameter (20,25). It consists of the RNA genome surrounded by a protein shell or capsid (15,23). ...

Reference:

Toxicity level of the tulathromycin on BHK cell culture and the effect on infective titers of foot and mouth disease viruses

... To detect SVDV in suspected material, the conventional diagnostic methods such as virus isolation (VI) and indirect-sandwich ELISA are routinely used in our laboratory. In 1995, we successfully applied RT-PCR for the detection of the SVDV genome in an infected cell culture (24). The period of SVDV persistence in clinical and tissue samples (nasal swabs, blood, faeces, epithelial tissue from vesicles) from experimentally infected pigs was investigated by conventional and nested PCR (RT-nPCR) assays (18). ...

Reference:

Occurrence and diagnosis of Swine vesicular disease: Past and present status

... These strains are now presented in most of the Middle Eastern countries and has reached since the beginning of 2009 Bahrain, Kuwait, Lebanon, Libya and Iraq. The new epidemic sub-lineage reported in the above countries and circulating (11) According to the FAO report 1999 the common serotypes isolated in Iraq from 1952 to 1998 were A, O Sat1 and Asia 1 (19) In 1988 Virus was isolation, serotyping and characterization of FMD disease in Iraqi Native Gazella (17). In 1998-2000 outbreak of the disease occur in Iraq, and the virus was isolated from Holstien cattle and characterized as O serotype. ...

Reference:

Diagnostic study of FMD virus in different area in Iraq

... The methods involving the use of sensitive cells are among those with the highest sensitivity and specificity (7,8). In the case of viral infections, studies involving cell cultures are considered the gold standard in diagnosis. ...

Reference:

Real-time replication of swine vesicular disease virus (SVDV) in cell culture systems in vitro

... The results observed in the present study are consistent with previously published case series where it is stated that the demyelinating form (AIDP) is the most prevalent in the country. 3,4,8,9 The results obtained in our study documented that 69% of cases correspond to demyelinating forms, 17% to axonal variants and 13% to other forms such as Miller-Fisher syndrome. These findings are notable because in the analyzed population the Miller-Fisher syndrome was a frequent variant (above the Colombian population average) that was lower than 1%. ...

Reference:

Clinical and neurophysiological characteristics of patients with Guillain ‐ Barré syndrome at Hospital Universitario San Ignacio, Bogotá, Colombia between 2009 and 2017

... In the scenario of overall distribution pattern of FMD virus types, serotype 'O' (60.00%) was the predominant type, followed by serotype 'A' (40.00%), (Table 3). specimens negative in sandwich ELISA test were found to be positive in RT-PCR.It indicates that the RT-PCR is more sensitive than and sandwich ELISA test (Donn et al., 1996;Reid et al., 1999;Alexandersen et al., 2000;Paprocka and Kesy, 2001;Paprocka et al., 2002;Clavijo et al., 2003;King et al., 2006;Verma et al., 2010Verma et al., , 2012). The higher sensitivity of RT-PCR may be because of its ability to detect very small number of virus as well as detection of RNA of non-viable FMD virus. ...

Reference:

Studies on Diagnosis of Foot and Mouth Disease by ELISA and Reverse Transcription Polymerase Chain Reaction in Bovines

... In the scenario of overall distribution pattern of FMD virus types, serotype 'O' (60.00%) was the predominant type, followed by serotype 'A' (40.00%), (Table 3). specimens negative in sandwich ELISA test were found to be positive in RT-PCR.It indicates that the RT-PCR is more sensitive than and sandwich ELISA test (Donn et al., 1996;Reid et al., 1999;Alexandersen et al., 2000;Paprocka and Kesy, 2001;Paprocka et al., 2002;Clavijo et al., 2003;King et al., 2006;Verma et al., 2010Verma et al., , 2012). The higher sensitivity of RT-PCR may be because of its ability to detect very small number of virus as well as detection of RNA of non-viable FMD virus. ...

Reference:

Studies on Diagnosis of Foot and Mouth Disease by ELISA and Reverse Transcription Polymerase Chain Reaction in Bovines

... For virological purpose, different ELISAs with specific monoclonal or polyclonal antibodies, haemagglutination assay (HA), direct immunoflurescence, and immune electron microscopy are used (1). In addition, several RT-PCR assays were developed and evaluated for the detection of RHDV based on nucleotide sequences of different genome fragments (3,7,11,13,14). ...

Reference:

Detection of RHD virus by a real-time reverse transcription PCR

... It consists of the RNA genome surrounded by a protein shell or capsid (15,23). It causes heavy economic losses to the livestock industry, such as a high morbidity in adult animals, treatment costs, reduced milk production, loss of working ability in draught animals in developing countries, reproductive disorders, and high mortality in young ones (13,19,24). The control of FMD is a national and regional responsibility, and, in many countries, the vaccine may be used only under the control of a veterinary authority (20,35). ...

Reference:

Toxicity level of the tulathromycin on BHK cell culture and the effect on infective titers of foot and mouth disease viruses

... Swine vesicular disease (SVD) is important due to its clinical resemblance to foot-and-mouth disease (16,17). Differential diagnosis of these two diseases is based on the detection of SVD antigen or on the demonstration of a specific antibody response and should be performed immediately after the appearance of vesicular changes (18). The virus neutralization test (VN) (11) and the monoclonal antibodybased ELISAs: competitive ELISA (MAC-ELISA) (1) and Ceditest ELISA (5) are routinely used for the examination of sera for SVDV antibodies. ...

Reference:

Diagnostic value of the isotype-specific ELISA for the detection of antibodies against swine vesicular disease virus (SVDV)