Fuyuko Yoshida's research while affiliated with National Institute of Health Sciences, Japan and other places

... Microcystis aeruginosa in natural water from India were also identified [42,43]. Srivastava et al. and Singh et al. [40,41] found concentrations of microcystins reaching a maximum of 1μgL -1 in lakes, ponds, and rivers of different geographical locations in central India, with reports from Germany and Portugal showing maximum toxin concentrations of 3μgL -1 [44] and water samples from Japan reaching 1μgL -1 in a number of different studies [45]. Studies report LC-MS for cyanotoxins in natural waters across Europe, including France, Italy, Ireland, and Germany, and describe microcystin concentrations <3 μgL -1 in tropic environmental conditions [47]. ...

... A part of this experiment was reported in a preliminary form (Tsutsumi et al., 1998b). ...

... More than 80 variants have been discovered with different structure and toxicity, where Microcystin-LR (MC-LR) is the most frequent and most lethal toxicants having LD 50 by intraperitoneal and oral route of approximately 25-150 µg/kg and 5 mg/kg, respectively [3]. It is a potent hepatotoxins and tumor-promoters which pose a serious threat to human health in the form of chronic expo-sure through drinking water [1,4]. MC-LR binds with protein phosphatase type 2A (PP2A) in an irreversible approach and inhibiting their enzymatic activity, results in tumor promotions and liver cancer [5,6]. ...

... MC-treated female and male fish in this study show the characteristic hepatocellular changes and the depletion of glycogen/ glycoprotein reserve that have been observed previously in fish Marie et al., 2012;Mezhoud et al., 2008) and mice (Guzman and Solter, 2002;Yoshida et al., 2001Yoshida et al., , 1998 acutely administered with MCs or MCcontaining cyanobacteria extracts. In fact, the observed liver damage in the present study is also similar to some of those described in the fish after chronic intoxication with MCs (Acuña et al., 2012;Qiao et al., 2016a;Trinchet et al., 2011). ...

... In this study, we observed a similar immune cell infiltration and aggregation in which CDHFD mice that were exposed to MC-LR showed significantly higher number of inflammatory foci as compared to Vehicle whereas those treated with the antioxidants showed significant reduction in inflammatory foci. Studies have shown the infiltration of immune cells such as macrophages, neutrophils and activation of the Kupffer cells in the liver tissues of mice and rats that were exposed to MC-LR [50,51]. He et al. has described that apart from inhibition of β-oxidation pathway, immune cell infiltration, inflammation, and fibrosis due to prolonged exposure to MC-LR are prominent processes observed in mouse hepatocytes [49]. ...

... The pathogenesis of MCLR hepatic injury begins with the uptake MCLR to hepatocyte probably mediated by bile acid transport system, which has been studied in isolated rat hepatocytes by using radiolabeled dehydro-MCLR 18,19 . Recently we reported the localization of MCLR in hepatocytes but not in non-parenchymal cells using a preembedding immunoelectron microscopy 20 . Relatively little, however, is known about how MCLR is distributed over the hepatic lobule. ...

... In risk assessment, the exact values and induced adverse effects should be elucidated before reporting the doses as PoD since none of the studies noticed water consumption. Ueno, inconsistent with their findings, did not report any lung effects in mice [42] receiving a low dose of MC-LR for 1.5 years via drinking water; in addition, the major organs did not show any alterations. Further studies are required to obtain a more conclusive result. ...

... As the interaction is primarily based on the binding of the two antibodies, anti-id-Abs have been found to be especially useful in the detection of toxic compounds, such as mycotoxins [7] and cyanotoxins [8]. In this study, deoxynivalenol (DON) was used as a model antigen for the generation of anti-Id-Abs with phage display selections. ...

... Microcystin concentrations reported here are well above average values reported in the majority of previous studies. Codd et al. found concentrations of microcystins reaching a maximum of 131 µg/L in water from the UK following analysis by HPLC [21], with reports from Germany and Portugal showing maximum toxin concentrations of 36 µg/L and 37 µg/L respectively [41] and water samples from Japan reaching 480, 1300, 15,600 and 19,500 µg/L in a number of different studies [41][42][43]. A recent report describing the application of a LC-MS/MS method for cyanotoxins in natural waters across Europe including France, Italy, Ireland, Germany, described microcystin concentrations <3 µg/L [44]. ...

... However, recent evidence suggests a substantial potential for inhalation exposure to MC-LR (May et al., 2018;Olson et al., 2020). In experimental animal models, intratracheal MC-LR exposure was found to have an LD50 approximately 140 times lower than oral exposure, indicating that the inhalation route of exposure to MC-LR may lead to drastically different events and adverse outcomes (Ito et al., 2001;Yoshida et al., 1997). In addition, there have been case reports and surveys demonstrating airway irritation after human exposure to MC-LR during recreational lake activities (Codd et al., 1999;Turner et al., 1990). ...