Francine Joly's research while affiliated with Centre de Recherche Pierre Fabre and other places

... Action de l'eau d'Avène sur les polynucléaires neutrophiles Au cours de la réaction allergique, les polynucléaires neutrophiles s'infiltrent dans la peau, sur le lieu de la réaction allergique cutanée"; ils sont alors prêts à interagir avec les kératinocytes, en libérant la substance P : celle-ci va provoquer l'adhésion des polynucléaires neutrophiles avec les kératinocytes en vue de propager la réaction allergique. Pour étudier l'effet de l'eau d'Avène sur la substance P, Beauvais et al. 29 Des mastocytes humains, ainsi que des mastocytes de rats, ont été stimulés par différentes substances allergisantes (Fig.17). En présence d'eau d'Avène dans le milieu, et avec une stimulation par des extraits allergisants, la dégranulation est significativement inhibée, par rapport à un milieu contenant simplement de l'eau distillée. ...

... Other authors have demonstrated that epinastine [43] and ketotifen [44] suppressed rabbit platelet aggregation induced by PAF at higher concentrations. Ketotifen also inhibited beta-hexosaminidase release from mouse mast cells derived from bone marrow [45]. ...

... Injections occurred daily when administration was delayed until 7 days following allogeneic BMT. The results demonstrated that post-treatment of nifuroxazide reduced the incidence and severity of mice with aGvHD, whereas a previous article reported that post-treatment using JAK2 inhibition ameliorated GvHD syndromes (32). Consequently, the histopathology The authors then investigated whether the immunosuppressive effects of nifuroxazide on GvHD, which blocked STAT3 activation, stemmed from the decrease of alloreactive T-cells. ...

... of PAF synthesis is the remodelling route; this pathway inkolves the activation of acetyltransferase, which acetylates lyso-PAF (l-O-alkyl-sn-glycero-3-phosphocholine), converting it to PAF (reviewed in reference [3]). A significant fraction of cellular PAF is secreted and transported in the circulation in association with albumin, although a minor fraction is associated with plasma lipoproteins [4]. ...

... It should also be noted that our previous studies have indicated that PMA-induced IL-6 and IL-8 production is similar in KBM and KBP cells, indicating that PMA effects are independent of the PAF-R (16). These findings fit with previous reports that phorbol esters do not induce PAF biosynthesis (25). The present studies indicate that activation of the NF-B pathway is responsible for PAF-R augmentation of chemotherapeutic agent-induced cytokine production (Fig. 8). ...

... All these events are prevented by specific PAF receptor antagonists, strengthening the presence of functional PAF receptors on these lymphoblastoid cells. Finally, PM amounts of PAF have been reported to induce superoxide anion generation in a human B-cell line (GLl) in vitro (Leca et al., 1990) and to increase by %-fold the expression of fibrinogen receptors on Raji cells (Levesque et al., 1986). Thus, in vitro, PAF appears to profoundly affect B-cell physiology, i.e. activation, proliferation and several functions. ...

... Our findings here and a number of earlier findings (35,36) indicate that PMA has little measurable impact on acetyltransferase activity. These observations disagree with those of Leyravaud et al. (37), who showed that stimulation with PMA not only increased neutrophil acetyltransferase activity but also raised PAF production. These differences may reflect varying assay conditions including different concentrations and times of incubations with PMA. ...

... Moreover, treatments of animals using MC stabilizers such as ketotifen and cromolyn are effective at limiting DENV-induced vascular leakage as measured by Evans blue and hematocrit assays (24). Mechanistically, ketotifen and cromolyn work by preventing the release of MC granules as well as other soluble mediators such as leukotrienes and plateletactivating factor (26)(27)(28)(29)(30)(31). A human clinical trial is under way to evaluate the effectiveness of ketotifen in inhibiting vascular leakage during DENV disease. ...

... Higher concentrations of lyso-PAF resulted in a drop of enzyme activity. This is consistent with previous data from our laboratory and others and probably due to detergent effect of lysophospholipids [20,37]. ...

... No subsequent experiments with purified preparations of the enzyme have been described, although solubilization of the enzyme with glycerol has been reported [23]. Lyso-PAF AT seems to be activated/inactivated by a phosphorylation/dephosphorylation mechanism [24,25,26,27,28,29]. AMP-dependent protein kinase, calcium calmodulindependent protein kinase, protein kinase C, and kinase p38 seem to be involved in the phosphorylation of the enzyme. ...