Filip Siska’s research while affiliated with Masaryk University and other places

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Publications (4)


Safety of tartrazine in the food industry and potential protective factors
  • Literature Review
  • Full-text available

September 2024

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61 Reads

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3 Citations

Heliyon

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Filip Siska

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Tartrazine belongs to the colors raising significant concerns regarding consumer safety at low doses relevant for real-life human exposure. Scientific literature continues to grow after the European Food Safety Authority (EFSA) re-evaluation in 2009 and the Joint FAO/WHO Expert Committee on Food Additives (JECFA) in 2016. Therefore, this review aims to collect recent knowledge on the toxicity issues of tartrazine, namely its genotoxicity, cytotoxicity, carcinogenicity, reproductive, developmental, and neurotoxicity, alterations of blood biochemical parameters, and hematotoxicity. The second part of the review covers the potential protective factors against the toxic effects of tartrazine based on the hypothesis of mitigation of oxidative stress induced by the color. The reviewed protective factors are crocin, royal jelly, fish oil, honey, acetylsalicylic acid, black caraway, blackthorn, turmeric, vitamin E, and riboflavin. This review concludes that tartrazine seems safe under the current acceptable daily intake (ADI) and the evidence on the potential protective factors is insufficient to reach any conclusion regarding their use.

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Description of colors and their regulatory evaluations.
Food Safety and Health Concerns of Synthetic Food Colors: An Update

June 2024

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173 Reads

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7 Citations

Toxics

The toxicity of food additives is widely studied and concerns many consumers worldwide. Synthetic food colors are often considered an unnecessary risk to consumer health. Since the European Food Safety Authority’s (EFSA) re-evaluation between 2009 and 2014, the body of scientific literature on food colors has grown, and new evaluations are being published by the Joint FAO/WHO Expert Committee on Food Additives (JECFA). Therefore, this narrative review aims to review the toxicological data that have become available since 2014. The reviewed colors are Quinoline Yellow, Sunset Yellow, Azorubine, Amaranth, Ponceau 4R, Erythrosine, Allura Red, Patent Blue, Indigo Carmine, Brilliant Blue FCF, Green S, Brilliant Black, Brown HT, and Lithol Rubine BK. Tartrazine was not included in this paper; the overwhelming amount of recent data on Tartrazine toxicity requires more space than this review can provide. The issues regarding the toxicity of synthetic food colors and real population exposures are being regularly examined and reviewed by relevant authorities, such as the EFSA and JECFA. The current ADI limits set by the authorities are mostly in agreement, and they seem safe. However, the EFSA and JECFA assessments of some of the colors are more than a decade old, and new evidence will soon be required.


Effects of low-dose alcohol exposure in adolescence on subsequent alcohol drinking in adulthood in a rat model of depression

April 2021

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42 Reads

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9 Citations

Filip Siska

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Objective Adolescence drinking and subsequent development of alcohol use disorder (AUD) is a worldwide health concern. In particular, mood dysregulation or early alcohol exposure can be the cause of heavy drinking in some individuals or a consequence of heavy drinking in others. Methods This study investigated the effects of voluntary alcohol intake during adolescence, i.e. continuous 10% alcohol access between postnatal days (PND) 29 to 43 and olfactory bulbectomy (OBX) model of depression (performed on PND 59) on alcohol drinking in Wistar rats during adulthood (PND 80-120, intermittent 20% alcohol access). In addition, the effect of NBQX, an AMPA/kainate receptor antagonist (5 mg/kg, IP) on spontaneous alcohol consumption was examined. Results Rats exposed to 10% alcohol during adolescence exhibited a lower 20% alcohol intake in the intermittent paradigm during adulthood, while the OBX-induced phenotype did not exert a significant effect on the drinking behavior. NBQX exerted a transient reduction on alcohol intake in the OBX rats. Conclusions Our results indicate that exposure to alcohol during adolescence can affect alcohol drinking in adulthood and that further exploration of AMPA and/or kainate receptor antagonists in co-morbid alcoholism-depression is warranted.


NBQX attenuates relapse of nicotine seeking but not nicotine and methamphetamine self-administration in rats

March 2021

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23 Reads

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6 Citations

Objective Pharmacological manipulations of glutamatergic ionotropic receptors have been suggested as a promising target for addiction treatment. Antagonists of AMPA/kainate receptors were shown to reduce alcohol intake or alcohol-seeking in various animal models. In this study, we evaluated the effect of NBQX, an AMPA/kainate receptor antagonist, on methamphetamine (METH) and nicotine self-administration in rats. Methods Male Wistar rats were trained to self-administer METH (0.08 mg/kg per infusion, session of 90 minutes) and nicotine (0.03 mg/kg per infusion, session of 60 minutes) under the fixed ratio 1 schedule of reinforcement. The maintenance training was two weeks. During the second week, NBQX was injected subcutaneously at doses of 5 or 10 mg/kg 20 minutes before the session or intravenously (IV) at doses of 1 and 5 mg/kg 10 minutes before the session. Following the maintenance training, rats were subjected to forced abstinence for two weeks and one day of the drug-free relapse-like session with IV NBQX treatment performed as before. Results Although NBQX did not affect nicotine maintenance, it significantly suppressed the drug-paired responding in the relapse session. Regarding METH, NBQX did not exert a significant effect at either phase of the study. Conclusions These findings suggest selective involvement of AMPA/kainate receptors in the relapse of nicotine seeking after a period of forced abstinence.

Citations (4)


... In the control group treated with physiological saline, normal cardiac architecture with intact intercalated discs and centrally located nuclei was observed. Similarly, no histological alterations were detected in groups treated with Tartrazine alone or in combination with N. sativa nanoparticles, as well as in the group treated solely with the nanoparticles (15). This suggests that, at the doses administered, neither Tartrazine no N. sativa nanoparticles induce cardiotoxic effects or alter the structural integrity of cardiac tissues in male albino mice (16,17). ...

Reference:

N. sativa seed Extract
Safety of tartrazine in the food industry and potential protective factors

Heliyon

... Discover Food (2025) 5:4 | https://doi.org/10.1007/s44187-025-00275-z of attention deficit hyperactivity disorder in children [3][4][5][6][7]. Consequently, the food industry is increasingly adopting for natural colorants as alternative to the synthetic colorant used in foods [2,8]. Natural colorants used in food are obtained from renewable resources such as plants, microbes and insects [9]. ...

Food Safety and Health Concerns of Synthetic Food Colors: An Update

Toxics

... Bilateral ablation of the olfactory bulbs under isoflurane anaesthesia was performed as previously described and repeatedly validated in the literature [22,[29][30][31][32][33][34]. In brief, two burr holes, 2 mm in diameter, were drilled in the frontal bone 7 mm anterior from the bregma and 2 mm lateral from the bregma. ...

Effects of low-dose alcohol exposure in adolescence on subsequent alcohol drinking in adulthood in a rat model of depression
  • Citing Article
  • April 2021

... Nicotine was purchased from Alomone Labs Ltd. (Jerusalem, Israel) as (−)-nicotine ditartrate and dissolved in saline to obtain a dose of 0.03 mg/kg in 0.1 mL (calculated as a free base). This dose is routinely used in nicotine self-administration studies (Fattore et al., 2009;Pushparaj et al., 2015;Boutros et al., 2016;Ruda-Kucerova et al., 2021). The maximum number of infusions in one session was not limited. ...

NBQX attenuates relapse of nicotine seeking but not nicotine and methamphetamine self-administration in rats
  • Citing Article
  • March 2021