Fernando Norambuena's research while affiliated with Deakin University and other places

Publications (26)

Article
Full-text available
Aquaculture, the fastest growing food production sector cannot continue to rely on finite stocks of marine fish as the primary source of the omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA), eicosapentaenoic acid (EPA; 20:5n3) and docosahexaenoic acid (DHA; 22:6n-3), for feeds. A four-month feeding trial was conducted to investigate t...
Article
Aim of study: The reproductive performance over a five year period in three different sole broodstocks: wild males with cultured females (WM), cultured males with wild females (CM), and a control of cultured males and females (C). Area of study: Cantabria, Northern Spain. Material and methods: The parental contribution was evaluated through the a...
Article
Full-text available
This study aimed to determine the nutritional composition, perceived quality and palatability of nine Australian seaweeds (wild-harvested species not yet exploited for human consumption; Laurencia filiformis, Codium galeatum, Cystophora polycystidea, Cystophora torulosa, Phyllotricha decipiens, Durvillaea potatorum, Hormosira banksii, Phyllospora c...
Article
Facing a bottleneck in the growth of aquaculture, and a gap in the supply and demand of the highly beneficial n -3 long-chain PUFA (LC-PUFA), sustainable alternatives to traditional marine-based feeds are required. Therefore, in the present trial, a novel oil obtained from a genetically engineered oilseed crop, Camelina sativa , that supplied over...
Article
Due to the scarcity of marine fish oil resources, the aquaculture industry is developing more efficient strategies for the utilization of dietary omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA). A better understanding of how fish utilize EPA and DHA, typically provided by fish oil, is needed. However, EPA and DHA have different physiol...
Article
Full-text available
Salmons raised in aquaculture farms around the world are increasingly subjected to sub-optimal environmental conditions, such as high water temperatures during summer seasons. Aerobic scope increases and lipid metabolism changes are known plasticity responses of fish for a better acclimation to high water temperature. The present study aimed at inv...
Article
Full-text available
Algae are at the base of the aquatic food chain, producing the food resources that fish are adapted to consume. Previous studies have proven that the inclusion of small amounts (<10% of the diet) of algae in fish feed (aquafeed) resulted in positive effects in growth performance and feed utilisation efficiency. Marine algae have also been shown to...
Article
Full-text available
Environmental and ecological conditions can shape the evolution of life history traits in many animals. Among such factors, food or nutrition availability can play an important evolutionary role in moderating an animal's life history traits, particularly sexually selected traits. Here, we test whether diet quantity and/or composition in the form of...
Conference Paper
Long chain polyunsaturated fatty acids are biologically active, with arachidonic acid (20:4n-6, ARA) and eicosapentaenoic acid (20:5n-3, EPA) being the two major omega-6 and omega-3 counterparts, respectively. The enzyme cyclooxygenase (COX-2) and 5-Lypooxygenase (ALOX-5) bioconvert ARA into the commonly termed "inflammatory eicosanoids", including...
Article
Full-text available
Teleost fish, as with all vertebrates, are capable of synthesizing cholesterol and as such have no dietary requirement for it. Thus, limited research has addressed the potential effects of dietary cholesterol in fish, even if fish meal and fish oil are increasingly replaced by vegetable alternatives in modern aquafeeds, resulting in progressively r...
Article
Previous studies on Senegalese sole (Solea senegalensis) indicated that cultured broodstock (first generation, G1) have lower tissue levels of arachidonic acid (20:4n-6, ARA) than wild counterparts. ARA is metabolized to form prostaglandins (PGs) that are involved in steroid production and follicle maturation in fish. In the present study the effec...
Article
The present study aimed to determine a non-invasive nocturnal lighting system for the behavioural observation of a highly light sensitive species, Senegalese sole (Solea senegalensis). Locomotor activity, four types of behaviour and plasma melatonin were analysed in groups of 12 adult Senegalese sole (Solea senegalensis) reared in captivity and hel...
Article
Full-text available
Previous studies have shown higher levels of arachidonic acid (20:4n-6, ARA) in testis, liver, and muscle of wild Senegalese sole (Solea senegalensis) compared to fish reared in captivity (first generation, G1). The present study was conducted to establish the optimal level of dietary ARA for G1 Senegalese sole broodstock, using as a reference the...
Article
Full-text available
Senegalese sole (Solea senegalensis) failed to reproduce and spawn viable eggs when bred and reared in cap-tivity (first generation G1 cultured broodstock). Cultured sole were also shown to have significantly lower levels of arachidonic fatty acid (20:4n−6, ARA) in reproductive tissues compared to wild counterparts. How-ever, to date no information...
Article
Prostaglandin levels in different tissues and cyclooxygenase (COX-2) gene expression were compared between wild and cultured Senegalese sole (Solea senegalensis) broodstock in which a significantly different fatty acid profile, particularly lower tissue levels of arachidonic acid (ARA, 20:4n-6) and higher levels of eicosapentaenoic acid (EPA, 20:5n...
Article
A complete reproductive failure to spawn viable eggs from G1 cultured Senegalese sole (Solea senegalensis) broodstock compared to successful spawning from wild caught captive broodstock has been described previously. The aim of the present study was to compare the proximate and fatty acid composition of recently caught mature wild versus mature G1...
Article
The objective of the study was to acclimatise wild-caught meagre (Argyrosomus regius) to captivity to produce viable eggs for aquaculture production. Twelve meagre (3 males and 9 females, mean weight = 20 ± 7 kg) were caught and transported to a land-based facility on 26 October 2006. During, March to June 2007, all three males were spermiating and...
Article
Twelve meagre (Argyrosomus regius) of mean weight 20 ± 7 kg were captured and acclimatized to captivity using environmental control and de-parasitizing protocols. An active feeding response and the occurrence of sexual maturation to advanced stages indicated that the fish adapted well to captivity. Spawning was induced with hormonal treatments, two...

Citations

... Animal feed Fish (Salmon) (Betancor et al., 2015a(Betancor et al., ,b, 2016b(Betancor et al., , 2017(Betancor et al., , 2018Hixson et al., 2014b;Wei et al., 2020;Xue et al., 2015) Fish (Trout) (Fraser et al., 2017;Hixson et al., 2014a;Koskela et al., 2021;Osmond et al., 2021;Wei et al., 2020) Fish (Cod) (Booman et al., 2014;Hixson et al., 2013Hixson et al., , 2016Morais et al., 2012;Tuziak et al., 2014) Fish (Tilapia) (Toyes-Vargas et al., 2020) Fish (Gilt-head bream) (Betancor et al., 2016;Huyben et al., 2020) Fish (European sea bass) (Betancor et al., 2021) Fish (European whitefish) (Suomela et al., 2017) Cows (Colombini et al., 2014;Hurtaud and Peyraud, 2007 Hernando et al., 2017) products that are a source of protein and best imitate the texture and taste of meat products, the food industry is also exploring other raw materials, such as gluten, pea proteins, and mycoproteins produced by filamentous fungi (Ismail et al., 2020). However, these raw materials carry potential nutritional threats of allergen or antinutritional ingredients, which the food industry must mitigate. ...
... The reproduction of Senegalese sole is extremely complex due to three singularities: the courtship behavior, the dominance and fidelity of highly successful spawners and the low production of sperm [15,23]. Taking into account these reproductive limitations, this study produced families by mass-spawning using a wild broodstock distributed in nine tanks after spawning synchronization by thermoperiod control [14,23]. ...
... Total ash was determined by incineration of samples in a muffle furnace at 550°C for 18 h (Atkinson et al., 1984). Carbohydrate content was included in the Nitrogen-free extract (NFE), and calculated by subtraction (Skrzypczyk et al., 2019;Eqn. 2): ...
... Animal feed Fish (Salmon) (Betancor et al., 2015a(Betancor et al., ,b, 2016b(Betancor et al., , 2017(Betancor et al., , 2018Hixson et al., 2014b;Wei et al., 2020;Xue et al., 2015) Fish (Trout) (Fraser et al., 2017;Hixson et al., 2014a;Koskela et al., 2021;Osmond et al., 2021;Wei et al., 2020) Fish (Cod) (Booman et al., 2014;Hixson et al., 2013Hixson et al., , 2016Morais et al., 2012;Tuziak et al., 2014) Fish (Tilapia) (Toyes-Vargas et al., 2020) Fish (Gilt-head bream) (Betancor et al., 2016;Huyben et al., 2020) Fish (European sea bass) (Betancor et al., 2021) Fish (European whitefish) (Suomela et al., 2017) Cows (Colombini et al., 2014;Hurtaud and Peyraud, 2007 Hernando et al., 2017) products that are a source of protein and best imitate the texture and taste of meat products, the food industry is also exploring other raw materials, such as gluten, pea proteins, and mycoproteins produced by filamentous fungi (Ismail et al., 2020). However, these raw materials carry potential nutritional threats of allergen or antinutritional ingredients, which the food industry must mitigate. ...
... Some studies have shown that Atlantic salmon have a more defined requirement for DHA than for EPA (Bou et al., 2017a;Emery et al., 2016;Glencross et al., 2014;Thomassen et al., 2012). In addition, there is evidence that salmon can be net producers of LC-PUFAs if they are deficient in the diet (Bou et al., 2017a;Ruyter et al., 2000a;Sanden et al., 2011). ...
... Reproductive failure to spawn viable eggs of Senegalese (Solea senegalensis) sole bred and reared in captivity (first generation G1, cultured broodstock) has been shown [5,7]. Wild counterparts, held in captivity, spawn regularly producing eggs of sufficient quality and quantity. ...
... Furthermore, low sperm membrane resistance to the seawater hyperosmotic effect is counteracted by S. senegalensis reproductive behavior. During spawning male and female swim in synchrony with the genital ducts held closely together (Duncan et al., 2008 ), increasing the probability of achieving fertilization by diminishing sperm scattering and the contact period with seawater. In the case of S. senegalensis, sensitiv-ity to hyperosmolarity is lower when a non-ionic solution is used, as reported by Martínez-Pastor et al. (2008) using a 1100 mOsm/kg sucrose solution, supporting the hypothesis of the importance of the above-mentioned reproductive behavior and the close releasing of both male and female gametes. ...
... The results indicate higher immune activity and possibly inflammation in the HDHA group and a difference in energy utilization between the two groups at the transcriptome level. While some studies have suggested a that inclusion of DHA alone is enough to satisfy the Atlantic salmon HUFA demand (Emery et al., 2016;Turchini et al., 2014) this study highlights the need for more knowledge of the individual role of the FAs, and that inclusion of dietary EPA can have several beneficial effects when Atlantic salmon are exposed to a challenging environment. ...
... In teleost species, the eicosanoid syntheses occur mainly in the liver [1], and several studies with marine fish species showed that n-6 and n-3 LC-PUFA compete for the same enzymes to synthesize eicosanoids [1,5,44,51]. These previous studies supported that the syntheses of these important lipid mediators are commonly related to the proportion of its precursors (EPA or ARA) in the diet [5,52]. ...
... In fish, ARA is mainly stored in polar lipids and is a minor component of fish cell membranes compared to DHA and EPA (Fountoulaki et al., 2003; Tocher, 2010). Recent research has focused on evaluating the importance of ARA in fish nutrition , with gradual elucidation of the physiological functions of the bioactive eicosanoids, fish performance and fatty acid digestibility (Alves Martins et al., 2012; Martinez-Rubio et al., 2013; Norambuena et al., 2012a; Norambuena et al., 2014; Trullàs et al., 2015 ). A growthpromoting activity of dietary ARA in different fish species, particularly during juvenile stages, has been reported. ...