April 2022
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5 Reads
We used the Kaneka Simple DNA Extraction Kit version 2 (Kaneka) for on-site DNA extraction from Sterivex. We injected 500µl of Solution A of the kit into the Sterivex and shook it by hand for 1min. We collected the Solution A buffer in the Sterivex by connecting the syringe with the connecting tube. We added 70µl of Solution B into the collected Solution A buffer in a 1.5-ml microtube and centrifuged (~2,000g) with a portable centrifuge for 3min. We collected 200µl of the supernatant solution.