F Beaujean's research while affiliated with Hôpital Henri Mondor (Hôpitaux Universitaires Henri Mondor) and other places

Publications (95)

Article
Rituximab induces clinical response in advanced B-cell lymphoma and is efficient in removing circulating B-cell from peripheral blood. We therefore postulated that rituximab might be a useful in vivo purging agent before high-dose therapy in this setting. Fourteen patients with relapsed follicular, marginal zone and mantle cell lymphomas (11, two a...
Article
Full-text available
In the previous LNH87-2 study, consolidative high-dose therapy followed by stem cell transplantation (HDT) improved disease-free survival, as well as survival for patients (pts) presenting with two or three factors of the age-adjusted international prognostic index (Aa-IPI) in first complete remission (CR). In order to improve further the outcome o...
Article
Previous studies demonstrated that ex vivo IL-2- activated PBSC could generate cytotoxic effectors without impairing haematopoietic reconstitution. Clinical experience and previous studies indicated that children with solid tumours could benefit from high-dose chemotherapy with immune modulation. We studied the generation of cytotoxic effectors fro...
Article
CD(34+)-enriched peripheral blood stem cells (PBSC) are increasingly being used as an autograft in patients with multiple myeloma (MM). The rationale for the use of the CD34+-enriched fraction in MM is the ability to obtain a graft with a significant reduction of contamination by plasma cells. However, the effect of such a manipulation on the proli...
Article
Conventional hematopoietic stem cell cryopreservation methods use a DMSO concentration of 10%. However, cells manipulated ex vivo may require more refined freezing protocols adapted to the specific cell suspension. In this retrospective study, we evaluated the results obtained with CD34+ cells purified from peripheral blood of 39 patients on the CE...
Article
Granulocyte-colony stimulating factor (G-CSF) has been successfully administered to healthy subjects to mobilize peripheral blood stem cells (PBSC) for allogeneic transplantation. Adverse events are moderate. We report the first case of apparent reactivation of an alloantibody to a blood cell antigen (Jk(a)) after G-CSF administration to a healthy...
Article
Full-text available
From 1987 to 1995, 22 children with refractory solid tumors entered a phase II study of high-dose thiotepa (HDT) (900 mg/m2) followed by stem cell transplantation (SCT) in the Pediatrics Department of the Institut Gustave Roussy. Tumor types were rhabdomyosarcoma (eight), osteosarcoma (seven), neuroblastoma (three), Ewing's sarcoma (three) and Burk...
Article
In an attempt to improve the poor prognosis of poor responders with stage IV neuroblastoma, a new combined high-dose chemotherapy conditioning regimen was tested. Event-free and overall survival, as well as the incidence of complications, were analysed. Twenty-five children aged 12-146 months at diagnosis entered this study. All were in complete re...
Article
Acute graft-versus-host disease (aGVHD) is still one of the main causes of morbidity and mortality after allogeneic bone marrow transplantation. Attempts to avoid GVHD are associated with an increased risk of relapse, probably because the graft-versus-leukemia effect is also abrogated. It was recently suggested that a high frequency of host-specifi...
Article
From July 1989 to December 1995, 16 children with metastatic Ewing's sarcoma or PNET were treated with high-dose chemotherapy (HDC) consisting of Busulfan (600 mg/m²) and Melphalan (140 mg/m²) followed by autologous SCT. Eight/16 were metastatic at diagnosis: 6/8 were consolidated in first intention and 2 after treatment of a metastatic relapse, 8/...
Article
Disease recurrence remains the major problem in autologous bone marrow transplantation (BMT) for hematologic malignancies. To improve the therapeutic efficiency of autologous BMT, we investigated the use of autologous marrow activated in vitro with interleukin 2 (IL-2) to generate killer cells for in vivo purging. A feasibility trial was initiated...
Article
Transfusion therapy for sickle cell anemia is limited by the development of antibodies to red cell antigens. The aim of this study was to evaluate whether transfusion of blood matched for antigens Rh and Kell would reduce the incidence of alloimmunization. We determined the transfusion history, red cell phenotype and development of alloantibodies i...
Article
Peripheral blood is becoming widely used as the only source of hematopoietic stem cells to support marrow ablative therapy in advanced lymphoma. We report data from 23 patients with high risk non-Hodgkin's (n = 19) and Hodgkin's lymphoma (n = 4) who underwent high-dose therapy with mobilized peripheral blood stem cell (PBSC) autografting. Periphera...
Article
The efficiency of stored platelet transfusion was evaluated in terms of clinical status in 141 thrombocytopenic patients. In a paired prospective study in which fresh platelets were used as controls, clinical efficiency was assessed on the basis of the ability to increase platelet count (recovery) and the time to the next transfusion (D). In 48 cli...
Article
Transfusion therapy for sickle cell anemia is limited by the development of antibodies to red cell antigens. The aim of this study was to evaluate whether transfusion of blood matched for antigens Rh and Kell would reduce the incidence of alloimmunization. We determined the transfusion history, red cell phenotype and development of alloantibodies i...
Chapter
No significant progress has been achieved in the treatment of multiple myeloma. Indeed, the reference combination Melphalan-Prednisone, introduced in 1970’s by Alexanian affords a median survival of between 20 and 44 months and between 20 and 26 months, exclusively for stage III disease [1].
Article
Forty-three patients with malignant nonmyeloid diseases underwent peripheral blood stem cell collections on an apheresis system (Spectra, COBE BCT, Lakewood, CO). Collections took place during the white cell (WBC) recovery phase following conditioning chemotherapy. One hundred two procedures were done after chemotherapy alone, and 72 procedures aft...
Article
The efficiency of stored platelet transfusion was evaluated in terms of clinical status in 136 thrombocytopenic patients. In a paired prospective study in which fresh platelets were used as controls, clinical efficiency was assessed on the basis of the ability to increase platelet count (recovery) and the interval to the next transfusion (D). In 48...
Article
The efficiency of strored platelet transfusion was evaluated in terms of clinical status in 136 thrombocytopenic patients. In a paired prospective study in which fresh platelets were used as controls, clinical efficiency was assessed on the basis of the ability to increase platelet count (recovery) and the interval to the next transfusion (D). In 4...
Article
The purpose of the present study was to evaluate the impact of frozen red cell transfusion on the transmission of hepatitis C virus (HCV) before the introduction of blood donor screening. Anti-HCV antibodies were detected in 59 patients with sickle-cell disease who required chronic transfusions and had exclusively received frozen red blood cells (R...
Article
We have studied the effect of recombinant human granulocyte colony-stimulating factor (G-CSF) on the growth of three different human neuroblastoma (NB) cell lines. using a clonal cell culture and a short proliferation test. The results have shown a lack of effect of G-CSF on the three cell lines. These data promote the use, in clinical trials, of G...
Article
The purpose of the present study was to evaluate the impact of frozen red cell transfusion on the transmission of hepatitis C virus (HCV) before the introduction of blood donor screening. Anti-HCV antibodies were detected in 59 patients with sickle-cell disease who required chronic transfusions and had exclusively received frozen red blood cells (R...
Article
The increased performance of white cell (WBC) filters makes it difficult to count precisely the number of residual WBCs. Concentrations as low as 0.01 WBC per microL cannot be determined with electronic cell counters, conventional hemocytometers, or the flow cytometric techniques currently being used. This article describes a simple, manual method...
Chapter
Allogeneic bone marrow transplantation following intensive myeloablative antileukemic therapy has proven an effective treatment for leukemias, and its curative effect is largely dependent on the graft-versus-host reaction [1, 2]. A high relapse rate is observed after autologous bone marrow transplantation (ABMT), possibly because of the presence of...
Article
To evaluate accurately the current performance of filtration, the French Produits Sanguins Labiles study group, composed of 21 transfusion teams, conducted a large-scale 6-month study involving over 1400 filtrations and 3000 controls. Some 745 standard red cell concentrates (RBC concentrates) and 690 concentrates previously white cell (WBC)-reduced...
Article
We describe our experience with a washing procedure used on cryopreserved, thawed bone marrow (BM) and peripheral blood stem cell (PBSC) grafts prior to autologous transplantation in 50 and 12 patients respectively. The procedure consists of a stepwise dilution with 2% human serum albumin and centrifugation performed either manually or using a bloo...
Article
Full-text available
Between April 1984 and May 1985, 17 heavily pretreated patients with relapsing or refractory germ cell tumours were treated with cisplatin 40 mg/m2/day, days 1-5; etoposide 350 mg/m2/day, days 1-5; cyclophosphamide 1600 mg/m2/day, days 2-5 and autologous bone marrow transplantation on day 8 as consolidation of conventional salvage chemotherapy. Non...
Article
The glutathione inhibitor drugs, 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and buthionine sulfoximine (BSO), were tested in vitro in order to assess their cytotoxic effectiveness when combined with an enzyme immunotoxin (eIT) composed of a T-cell reactive monoclonal antibody (mAb) 097 coupled to the reactive oxygen-generating enzyme, glucose oxid...
Article
In recent years, the prognosis of non-seminomatous germ-cell tumors has been greatly improved by the use of novel chemotherapeutic regimens including platinum derivatives. However, the prognosis remains poor for a certain proportion of these patients. We have therefore developed an intensive chemotherapy protocol (PEC) followed by bone marrow autog...
Article
Thirty-two children were treated for metastatic Ewing sarcoma by high-dose chemotherapy followed by autologous bone marrow transplantation. Fourteen patients entered phase II studies of high-dose alkylating agents. The response rate observed was 61%. High-dose chemotherapy was used as consolidation in complete remission of 18 patients. Disease free...
Chapter
Increasing evidence indicates that natural killer (NK) cells may play a role in defense against leukemia [1, 2]: low NK responding individuals have an increased susceptibility to leukemia; patients with acute leukemia (AL), even in complete remission (CR), have a defect in NK cell function [3,4]; and NK clones with antileukemia activity have been o...
Chapter
Autologous bone marrow transplantation (AMBT) is widely used to circumvent the myelotoxicity of intensive therapeutic regimens. Numerous clinical results provide evidence that autografting limits myelosuppression [1] and/or reconstitutes the host’s hemopoietic system after lethal marrow injury [2]. Studies in recent years have demonstrated that rec...
Article
Twenty-nine children (median age: 41 months) with advanced solid tumors received, as consolidation therapy, two consecutive courses of high-dose chemotherapy (HDC) followed by mafosfamide-purged autologous marrow transplantation (ABMT) with a 3- to 4-month interval between each course. The malignancies were neuroblastoma (n = 22), Ewing's sarcoma (...
Article
CD18 antibodies react with the common beta chain of the human leukocyte function antigen (LFA1)* and thus block the functions mediated by the three identified molecules in humans. A murine CD18 monoclonal antibody was infused in 8 leukemic patients receiving allogeneic T-depleted bone marrow transplantation in order to prevent graft rejection. This...
Article
Between February 1979 and May 1986, 165 children were treated with autologous bone marrow transplantation after high-dose chemotherapy without total-body irradiation for a hematologic malignancy or a solid tumor. Nonbacterial nonfungal interstitial pneumonitis was observed in 24 children and was the cause of death in 11 cases. Of the 24 cases of in...
Article
Among 62 children over 1 year of age at diagnosis who were treated for stage IV neuroblastoma, 33 entered remission after conventional therapy. They received high-dose chemotherapy and in vitro purged bone marrow transplantation as consolidation therapy. Fifteen patients received one course and 18 two courses. At present, 16/33 grafted patients are...
Article
The efficacy of bone marrow transplant (BMT) T-cell depletion for the prevention of acute graft-versus-host disease (GVHD) has been demonstrated in animal models and in clinical studies. The importance of T-cell depletion has to be evaluated with standardized methods suitable for routine purposes. We report herein an in vitro mature T-cell depletio...
Article
The morphologic and functional properties of platelets after irradiation with 2500 rads and storage, in first-generation containers, for 48 h in a liquid phase at 20 degrees C with continuous horizontal agitation have been analyzed and compared with a control group of the same platelets which were not irradiated. The preservation technique induced...
Article
Among 62 children over 1 year of age at diagnosis, who were treated for stage IV neuroblastoma, 33 entered complete remission (CR) or good partial remission (GPR) after conventional therapy and received high-dose chemotherapy (HDC) with in vitro purged autologous bone marrow transplantation (ABMT) as consolidation therapy. The HDC was a combination...
Article
The morphologic and functional properties of platelets after irradiation with 2500 rads and storage, infirst-generation containers, for 48h in a liquid phase at 20°C with continuous horizontal agitation have been analyzed and compared with a control group of the same platelets which were not irradiated. The preservation technique induced changes in...
Article
In order to evaluate the effectiveness and reproducibility of T cell depletion in human leukocyte antigen (HLA)-matched bone marrow graft to prevent graft-v-host disease (GVHD), our multicentric study (nine different centers) investigated 62 consecutive patients with poor prognosis leukemia or hematosarcoma from June 1984 to November 1985. The data...
Article
Nineteen patients were treated with high dose chemotherapy followed by an autologous bone marrow transplantation. The chemotherapy regimen was an association of BCNU, procarbazine, etoposide, melphalan. Six patients had a progressive disease; 4 short-term complete remissions were observed. In 13 responding patients, 6 maintained complete remissions...
Article
Current studies suggest that the depletion of T-lymphocytes from donor marrow is an effective method for preventing acute graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation in man. To deplete the T-lymphocytes from bone marrow cells we use either monoclonal anti-T-cell antibodies and complement or T101 ricin A-chain immun...
Article
Twenty children with advanced, nonleukemic malignancies entered a phase II study of high-dose busulfan-cyclophosphamide followed by bone marrow transplantation (BMT). All had disease refractory to conventional and/or high-dose chemotherapy (HDC). There were ten neuroblastoma patients, six non-Hodgkin's lymphoma, three Ewing's sarcoma, and one rhabd...
Article
Twenty-four poor-risk patients with acute lymphoblastic leukemia received a modified regimen of carmustine, cytarabine, cyclophosphamide, and 6-thioguanine (BACT) followed by autologous bone marrow transplantation (ABMT). Nineteen patients were in second or subsequent complete remission (CR) when treated with this regimen; 3 died early, 2 died of p...
Article
Dose-effect relationships of high-dose melphalan were evaluated in 37 patients with measurable relapsed or refractory acute leukemias. Thirteen patients (Group 1) received 70-100 mg/m2 of melphalan without marrow rescue and 24 patients (Group 2) received 140-180 mg/m2 of melphalan followed by marrow transplantation. Patients in both groups were com...
Article
Fifteen children with advanced neuroblastoma according to Evans' classification (1 with stage III and 14 with stage IV) were treated with high-dose melphalan (HDM) followed by autologous bone marrow transplantation. Before HDM, all patients had been extensively treated with multimodality therapy for a median duration of 9 months. At the time of HDM...
Article
Twenty-nine patients were treated with single or combined high-dose melphalan therapy followed by autologous bone marrow transplantation. Hematopoietic recovery from these treatments was studied. No correlation was found between the number of GM-CFC infused and the time required for hematopoietic recovery. It is suggested that this correlation is o...
Article
Sixteen children with non-Hodgkin's lymphoma (NHL) who had relapsed were treated with high-dose chemotherapy with BCNU, cyclophosphamide, cytarabine, 6-thioguanine (high-dose chemotherapy [HDC]) and autologous bone marrow transplantation (ABMT). Eleven complete responses were obtained and five patients remain in prolonged complete unmaintained remi...
Article
Cryopreservation of human bone marrow may be helpful to use supralethal chemoradiotherapy in order to cure malignant diseases. We report here a cryopreservation procedure from large volumes of human bone marrow which can be applied in routinal use. Whole bone marrow in 10% Dimethylsulfoxide (ME2SO) was frozen at an 1 degree C/min. controlled rate a...
Article
A method of red cell preservation by freezing at -25 degrees C is described. Glycerol is added to the red blood cells in the primary polyvinyl chloride plastic collection bag to achieve a concentration of 28 pr cent (W/V). The blood cells are concentrated by centrifugation and the supernatant glycerol is discarded. Glycerolized red cells are frozen...
Article
A method of red cell preservation by freezing at −25°C is described. Glycerol is added to the red blood cells in the primary polyvinyl chloride plastic collection bag to achieve a concentration of 28 pr cent (W/V). The blood cells are concentrated by centrifugation and the supernatant glycerol is discarded. Glycerolized red cells are frozen and sto...
Article
The effectiveness of transfusions of frozen red cells has presently been clearly demonstrated. The authors present their technique of preparation of these cells and their experience of this material in 51 cases of ECC. The results observed plead in favour of the notion that frozen-defrosted blood, combines the advantages of washed blood, freed from...
Article
The freezing of blood permits preservation of red cells over long periods of time, several months or years. Leucocyte and platelet contamination of red cell concentrates to be frozen is negligible. The amount of the various red cell metabolites (2.3 D.P.G., A.T.P., etc.) is maintained. Washing of thawed red cells removes the remaining plasma protei...
Article
The freezing of blood permits preservation of red cells over long periods of time, several months or years. Leucocyte and platelet contamination of red cell concentrates to be frozen is negligible. The amount of the various red cell metabolites (2.3 D.P.G., A.T.P., etc.) is maintained. Washing of thawed red cells removes the remaining plasma protei...
Article
A successfull bone marrow transplant was achieved in a case of post hepatitic aplastic anemia after cyclophosphamide immunosuppression. Caryotype analysis, erythrocytic phenotype and IgG Gm allotype demonstrated evidence of complete chimerism. Anti-thymocyte serum undoubtly was able to suppress a life threatening episode of graft versus host reacti...
Article
Disseminated intravascular coagulation (DIVC) in acute leukemia may be abolished by chemotherapy alone. However, in some cases the symptoms of DIVC can be temporarily exacerbated. Death often occurs from brain hemorrhage. With the combined use of the heparin and chemotherapy, biological symptoms of DIVC disappear more rapidly but cerebral hemorrhag...

Citations

... The value of ovarian tissue grafting for cancer patients is debatable. Viruses, including HIV353637 and hepatitis virus, [38,39]and cancers [40]can be transmitted by grafts, and cancers have been known to recur in patients in remission after the replacement of autologous cryopreserved bone marrow.414243 It is therefore possible that bloodborne cancers such as leukaemia, systemic cancers such as lymphoma and metastasising cancers could be transmitted by ovarian tissue grafts. ...
... Studies comparing the mobilization of PBPC in patients with different malignancies have indicated that PBPC harvesting might be more difficult in MM patients than in patients with other malignancies (Haas et al, 1994;Norol et al, 1994;Bensinger et al, 1996). Several modalities for the mobilization of progenitor cells are under investigation. ...
... So far the DMSO method has shown to be the most promising, with freezing and storage in the vapor phase of liquid nitrogen. 44,45 Clinical use is largely indicated for malignancies during the aplastic phase using pre-collected autologous platelets. ...
... The minimum detection limit for PLTs was 1x10 9 /l with the automated blood count and 0.5x10 9 /l with microscopic counting. Microscopic counting of the leukocytes (Nageotte chamber [13]) was performed. ...
... 18 Hematopoietic support SC transplantation was performed 24-48 h after the last dose of HDC. Hematopoietic support was initially performed with purged (ASTA Z) or unpurged autologous BM. 19 Since 1994, PBSC have been collected by leukapheresis either after mobilization with conventional chemotherapy and G-CSF at 5 μg/kg or in steady state with G-CSF at 10 μg/kg. A total of 3 × 10 6 CD34+ cells/kg had to be collected and cryopreserved to allow the administration of HDC. ...
... 29,30,31,32,33,34 In France, the rate was about 30%. 35,36 Despite the mandatory donor and recipient Rh-Kell antigens matching before transfusion in these developed countries, alloimmunisation rates in those settings are higher than ours. 34,37,38 This serves as a reminder that the risk of alloimmunisation is multiparametric, depending on the population's subgrouping or prevalent diseases. ...
... 24,25 High-dose chemotherapy has been demonstrated to improve the prognosis of these patients. 26 The phase II study of HDT requires a larger accrual to determine whether this drug is effective in Ewing's sarcomas. ...
... Priming is usually performed with concentrated red blood cells (RBC) but combinations of RBC, human serum albumin (HSA) and NaCl are also used by some teams. 18,43,44,52,53 Brault et al 53 reported satisfactory priming of the extracorporeal volume with fluid gelatin alone. Obviously, RBC units must be cross-matched, irradiated and leukocyte-depleted. ...