Erik Odeblad's research while affiliated with KTH Royal Institute of Technology and other places
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Publications (93)
BS>The distribution and kinetics of Na²² in mice and rabbits were ; studied autoradiographically and by scintillation counting at various intervals ; after an intravenous injection of Na²² in an isotonic solution. The amount ; of Na²² after the steady state was reached was highest in teeth among the ; various organs and tissues studied and de...
RESULTS have recently been reported concerning the proton magnetic resonance line-width of the water phase of starch gels1. I have examined the solvent line-width of gelatin gels at 16.7 Mc./sec. in a field of 3,910 gauss with the spectrometer now in use at this laboratory. The samples were contained in soda glass tubes of 1.2 mm. inside diameter....
The uptake of Co60Cl2 in the ovary of the rat and the cat was observed. The uptake is largely localized in the follicular granulosa. The possible significance of this finding is discussed.
The effect of inhomogeneity on the transmission of beta rays was
studied, and a simplified theory is presented. Experimental observations
illustrate the theory. The effect of nuclear elastic scattering of the absorbing
nuaterial is considered, and an approximate method for evaluating the maximum
range of beta rays and the exponent of the binomi...
Methods are described for determining the proton magnetic resonance
spectra and proton magnetic spinlattice relaxation time of human milk at various
periods after childbirth. (C.H.)
The technique for the determination of the sodium and phosphorus in
human cervical mucus with activation analysis is described. The amount of Na was
found to be relatively constant but the amount of P was directly correlated to
the amount of dry substance. (auth)
Approximate and simplified theoretical considerations result in the ; binominal formula proposed earlier for describing tramsmission of beta rays. ; Experimental measurements on 18 radionuclides are presented and support the ; preference for the binominal formula rather than the experential formula. The ; dependence of the exponent in the binominal...
The 27Al (a, n) 30P reaction has been used to detect Al in different specimens. A210Po alpha source was used. The over-all method error in one determination was about 5 per cent. Binary systems containing Al are expected to be most favorable for analysisRésuméLa réaction nucléaire 27Al (a, n) 30P a été utilisée pour le dosage de l'aluminium dans di...
Excerpt
As a continuation of previous studies on ovarian arteriovenous difference measurements (Odeblad, 1951, Odeblad & Enhörning, 1953, Odeblad, Scrosoppi & Enhörning, 1955), we have performed some measurements on the absolute and relative circulation through the ovary of the rabbit. Since Kety (1949) and Walder (1954) have shown a certain relati...
. Subcutaneously injected Fe59Cl3 in mice accumulates to a fairly large extent in the kidneys. The first few days after injection several structures (except glomeruli) in the cortex contained much radioiron. Nine days after injection the radioiron was mainly localized in proximal convoluted tubules. The marrow contained small amounts of radioiron t...
Iron and cobalt are elements of considerable biologic and medical interest and their metabolism may be conveniently studied with the aid of the radioisotopes CoB0 and Fe59. If these isotopes are used together in 'double' tracer experiments, their individual determination is difficult due to the very similar energies of their beta and gamma radiatio...
Excerpt
In the present work we have studied the uptake of radiophosphate and radiosulphate in the mouse ovary at various ages and after the administration of pregnant mare serum gonadotrophin (SG).⁴ It has been shown previously in the rabbit that the follicular granulosa has a rather high ability to concentrate radioactive phosphate (Odeblad, 1952)...
Ignoring the complicated mechanisms governing the absorption of beta rays (reduction of the energy of single beta particles, complete arrest of low-energetic beta particles, elastic and inelastic scattering, shape of beta spectrum etc.) it is sometimes useful to describe the study of the phenomena in over-all transmission or absorption in simple ma...
The uptake of radioselenium after injection of Se ⁷⁵ ‐labelled sodium selenate in some tissues known to contain sulpho‐muco‐Polysaccharides was studied by means of autoradiography. When the results obtained were compared to what is known about the distribution of S ³⁵ after administration of labelled sodium sulphate, it was found that the selenate...
The unfertilized rabbit ovum takes up only small amounts of labeled sulfate in its cytoplasm, as demonstrated by autoradiography.
The ovaries of mice in various cyclic phases, of mice injected with P32 at different points of time before death and of mice given different amounts of P32 were studied as regards the possibility of obtaining quantitative methods of studying the irradiation effects on the follicles. The general appearance of the follicles in histological sections,...
Excerpt
The development of mammary glands at puberty and in pregnancy as well as experimentally suggests, that these glands have the ability to respond to oestrogen stimulation, though other factors may also be involved (for references see Burrows, 1949). Previous experimental work on mammary growth has not given any direct information on the prima...
Nine white albino rats were given S35 and autoradiographs were prepared from trachea. Controlled and standardized development of the film enabled quantitative measurement of S35 by autoradiography.The amount of S35 at different intervals after injection is given in Fig. 2. A sharp peak appeared 8 hr. after injection. After 24 hr. there was a slow d...
Excerpt
The presence of mucus or mucoid substances in different parts of the female genital organs, particularly in the ovarian cysts and in the uterus is well known. If mucopolysaccharides are esterified with sulfuric acid, their presence can be demonstrated by means of autoradiography after the introduction of radioactive sulfur.
In previous inve...
By means of autoradiography, permitting a resolution of about 25 μ, it is indicated that acid- and lipid-insoluble P32 is present only in small amounts in unfertilized, nearly mature rabbit ova. No changes are indicated in treatment with human chorionic gonadotrophin or in pregnancy.
Citations
... 24 Additionally, autoradiographic methods have been used. 25 All of these techniques have been applied to describe the morphologic aspects of dentin PG. [26][27][28][29] Recently an immunocytochemical approach has been proposed at the light microscopy level. 9 The aim of this research project was to identify the human dentin PG following different application times of a solution of 35% PA (and, as control specimen, after conditioning with EDTA at pH 7.0) by means of an immunocytochemical technique with high-resolution SEM. ...
... A transfer of radiosulphate from the maternal rat to the foetus was first reported by Layton et al. (1950). Bostrom & Odeblad (1953) briefly reported the incorporation of radiosulphate 1 A preliminary report on this subject appeared in Experientia, 10, (1954), 67. by rabbit embryos as revealed by autoradiography. The uptake of S 35 varied markedly in different tissues, being highest in those presumably containing sulpho-mucopolysaccharides. ...
... They hypothesized secretion of iron-rich materials from the glands into the uterine lumen from where the yolk sac endoderm absorbed the compounds. Subsequent physiological studies have indicated the yolk sac has at least some role in maternal-fetal transfer of iron (Nylander, 1953;Magnusson et al, 1955;Glasser et al, 1968;Kaufman and Wyllie, 1970;Garrett et al, 1972Garrett et al, , 1973. The relative importance of the yolk sac in iron transfer compared with the chorioallantoic placenta is the subject of some disagreement in the above-mentioned studies, perhaps due in part to variations in gestational age and to the form in which iron was administered. ...
... Information as to the penetration of chemicals into the granulosa cells and into the oocyte is scanty, although there is some evidence that these structures are also permeable to foreign chemicals (18)(19)(20). Moreover, Glass (16) has shown that albumin and a-globulin penetrate the oocyte, but that,8and y-globulins do not. ...
... Radioautography of rat mast cells has been utilized to show that sulfate is incorporated into heparin and then slowly lost. Approximately 75% of the heparin was found to be present after 18 days [50]. When ['4C]hyaluronic acid was injected into guinea pigs [51], most of the label was found to be excreted as hyaluronic acid with less being metabolized. ...
... 2) Apposition techniques with separate processing of tissue-containing slide and photographic emulsion-containing slide (Hoecker and Roofe, 1949;Williams, 1951;Magnusson and Odeblad, 1954;Oster et al, 1955;Sterling and Chichester, 1956;Huang, 1960;Novek, 1962;Werner et al, 1966). ...
... A number of applications have been proposed for scatter images. Because the intensity of the image is proportional to electron density, researchers proposed (Odeblad and Norhagen 1956) and showed (Lale 1959, Farmer and Collins 1971, Clarke et al 1976, Harding and Tischler 1986) that scatter images may be used to create volumetric electron density maps of 3D objects and tissues, although steps must be taken to reduce the contribution from multiple scattering (Farmer and Collins 1971) and correct for attenuation (Lale 1959, Clarke and Van Dyk 1973, Battista et al 1977. The high fluence (and resulting dose) required for these measurements and the development of kilovoltage computed tomography (CT) made scatter electron density mapping obsolete (Battista and Bronskill 1981). ...
... The latter takes into account the absorption of radiations, primarily aimed at the crystal, and the scattering toward the crystal of radiations primarily directed elsewhere. 20 As shown in figure 1, -(v) decreases as the longitudinal distance between the source and the counter increases. Lateral displacement of the source from the axis of the counter also decreases q (v). ...
... In 1956, Scandinavian gynaecologist Odeblad was the first physician to utilize NMR spectroscopy for the analysis of a reproductive fluid. He retrieved cervical mucus from apparently healthy women and using 1 H-NMR spectroscopy demonstrated changes in an unknown signal at different stages of the menstrual cycle (Odeblad and Bryhn, 1957). The authors postulated that this change was in response to estrogenic changes (Odeblad and Bryhn, 1957). ...
... Nuclear magnetic resonance (NMR) spectroscopy has proven to be a powerful tool for the analysis of milk and dairy products, given its unique ability to simultaneously detect and identify small molecules within complex matrices, such as milk [1]. NMR was initially employed by Odeblad and Westing in the 1950s to investigate milk properties and allowed the identification of distinct components of human milk: lactose from milk plasma and milk fat [2]. Since then, NMR spectroscopy has been extensively utilized in various studies, providing valuable insights into the composition, structure, and properties of milk, including the content of key components such as fat, protein, lactose, and minerals. ...