Erik J Suuronen's research while affiliated with University of Ottawa and other places

Publications (204)

Article
Full-text available
Methylglyoxal (MG) production after myocardial infarction (MI) leads to advanced glycation end‐product formation, adverse remodeling, and loss of cardiac function. The extracellular matrix (ECM) is a main target for MG glycation. This suggests that ECM‐mimicking biomaterial therapies may protect the post‐MI environment by removing MG. In this study...
Article
Full-text available
Cardiomyocytes are the muscle cells that make the heart beat, pump oxygen, and deliver nutrient-rich blood throughout the human body. During a heart attack, the blood supply to the heart is interrupted. Cardiomyocytes then die and are replaced by scar tissue that can no longer contract. As a result, the heart is weakened and may beat abnormally. Fo...
Article
Full-text available
Bioprinting has rapidly progressed over the past decade. One branch of bioprinting known as in situ bioprinting has benefitted considerably from innovations in biofabrication. Unlike ex situ bioprinting, in situ bioprinting allows for biomaterials to be printed directly into or onto the target tissue/organ, eliminating the need to transfer pre-made...
Chapter
Biomaterials are scaffolds designed to mimic the extracellular matrix and stimulate tissue repair. Biomaterial therapies have shown promise for improving wound healing in cardiac tissue after ischemic injury. An unintentional consequence of biomaterial delivery may be the stimulation of inflammation through recruitment of circulating monocytes into...
Article
Full-text available
We report the development, as well as the in vitro and in vivo testing, of a sprayable nanotherapeutic that uses surface engineered custom-designed multiarmed peptide grafted nanogold for on-the-spot coating of an infarcted myocardial surface. When applied to mouse hearts, 1 week after infarction, the spray-on treatment resulted in an increase in c...
Article
Full-text available
Methylglyoxal (MG) is a highly reactive dicarbonyl and the main precursor of advanced glycation end‐products (AGEs). After myocardial infarction (MI), MG‐derived AGEs accumulate in the heart and contribute to adverse remodeling and loss of cardiac function. In this study, the flavonoid fisetin, a dicarbonyl scavenger, is used to reduce the negative...
Article
The goal of this study was to develop strategies to localize human collagen-based hydrogels within an infarcted mouse heart, as well as analyze its impact on endogenous extracellular matrix (ECM) remodeling. Collagen is a natural polymer that is abundantly used in bioengineered hydrogels because of its biocompatibility, cell permeability, and biode...
Article
Background: Wnt signaling plays a critical role in both embryonic cardiogenesis and cardiac remodeling in adult heart disease. We have previously demonstrated that the canonical Wnt/β-catenin pathway inhibits cardiac sodium current, but it remains unclear whether the noncanonical Wnt pathway affects cardiac electrophysiology. Methods and Results: W...
Article
Purpose of review: This review describes the latest advances in cell therapy, biomaterials and 3D bioprinting for the treatment of cardiovascular disease. Recent findings: Cell therapies offer the greatest benefit for patients suffering from chronic ischemic and nonischemic cardiomyopathy. Rather than replacing lost cardiomyocytes, the effects o...
Article
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Biofilm formation in living organisms is associated to tissue and implant infections and it has also been linked to the contribution of antibiotic resistance. Thus, understanding biofilm development and being able to mimic such processes is vital for the successful development of anti-biofilm treatments and therapies. Several decades of research ha...
Article
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Outflow tract abnormalities are the most frequent congenital heart defects. These are due to the absence or dysfunction of the two main cell types, i.e., neural crest cells and secondary heart field cells that migrate in opposite directions at the same stage of development. These cells directly govern aortic arch patterning and development, ascendi...
Article
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Rapid synthesis of nanomaterials in scalable quantities is critical for accelerating the discovery and commercial translation of nanoscale-based technologies. The synthesis of metal nanogold and silver in volumes larger than 100 mL is not automatized and might require of the use of harsh conditions that in most cases is detrimental for the producti...
Article
Cardiovascular diseases are the leading cause of mortality worldwide. Given the limited endogenous regenerative capabilities of cardiac tissue, patient-specific anatomy, challenges in treatment options, and shortage of donor tissues for transplantation, there is an urgent need for novel approaches in cardiac tissue repair. 3D bioprinting is a techn...
Preprint
Full-text available
Rapid synthesis of nanomaterials in scalable quantities is critical for accelerating discovery and commercial translation of nanoscale-based technologies. The synthesis of metal nanogold and silver in volumes larger than 100 mL is not automatized and might require of the use of harsh conditions that in most cases is detrimental for the production o...
Article
Injectable hydrogels are a promising method to enhance repair in the heart after myocardial infarction (MI). However, few studies have compared different strategies for the application of biomaterial treatments. In this study, we use a clinically relevant mouse MI model to assess the therapeutic efficacy of different treatment protocols for intramy...
Article
As cell therapies emerged, it was quickly realized that pro-regenerative cells directly injected into injured tissue struggled within the inflammatory microenvironment. By using microencapsulation, i.e. encapsulating cells within polymeric biomaterials, they are henceforth protected from the harmful extracellular cues, while still being able to rec...
Article
Full-text available
While cell therapy is emerging as a promising option for patients with ischemic cardiomyopathy (ICM), the influence of advanced donor age and a history of ischemic injury on the reparative performance of these cells are not well defined. As such, intrinsic changes that result from advanced donor age and ischemia are explored in hopes of identifying...
Article
Full-text available
Despite regenerative medicine (RM) being one of the hottest topics in biotechnology for the past three decades, it is generally acknowledged that the field's performance at the bed‐side has been somewhat disappointing. This may be linked to the novelty of these technologies and their disruptive nature, which has brought an increasing level of compl...
Article
A novel strategy is needed for treating non-healing wounds that is able to simultaneously eradicate pathogenic bacteria and promote tissue regeneration. This would improve patient outcome and reduce the number of lower limb amputations. In this work, we present a multifunctional therapeutic approach able to control bacterial infection, provide a pr...
Article
Using chemically modified extracellular matrix proteins such as collagen, in combination with light for tissue bonding reduces inflammation and minimizes scarring. However, full length animal or recombinant human collagen proteins are difficult to isolate/produce. Thus, short biomimetic collagen peptides with properties equivalent to collagen at bo...
Chapter
Cardiovascular disease is a leading cause of worldwide mortality. Despite the success of current therapies for acute myocardial infarction (MI), many patients still suffer irreversible damage, and the prevalence of heart failure is growing. After MI, the extracellular matrix (ECM) of the damaged myocardium is modified to produce scar tissue. This r...
Article
Full-text available
Despite the success of current therapies for acute myocardial infarction (MI), many patients still develop adverse cardiac remodeling and heart failure. With the growing prevalence of heart failure, a new therapy is needed that can prevent remodeling and support tissue repair. Herein, we report on injectable recombinant human collagen type I (rHCI)...
Article
Full-text available
New antimicrobial textiles were prepared through direct chemical linkage of bioactive molecules eugenol and fluoroquinolone derivatives, onto the surface of cotton fabrics. The attachment through a triazine moiety minimizes the leaching of the antimicrobial molecule into the surroundings of the material. Bacterial efficacy against Staphylococcus au...
Article
Coronary artery disease is a leading cause of death in developed nations. As the disease progresses, myocardial infarction can occur leaving areas of dead tissue in the heart. To compensate, the body initiates its own repair/regenerative response in an attempt to restore function to the heart. These efforts serve as inspiration to researchers who a...
Chapter
The biomedical properties of nanoparticulated silver have been widely studied in the last decade; however, still there are concerns regarding its long-term toxicity. Recent developments in nanoparticle fabrication and surface manipulation, using different biomolecules, have allowed the preparation of nontoxic nanosilver. Thus, nanosilver has been s...
Article
In many diseases, tissue regeneration is compromised and/or insufficient to restore tissue/organ function. Therefore, novel regenerative therapies are being developed to enhance resident and transplanted cell proliferation and functional differentiation. Numerous biomaterials engineered to include nanocomponents have emerged as promising candidates...
Article
CD34+ cells are promising for revascularization therapy, but their clinical use is limited by low cell counts, poor engraftment, and reduced function after transplantation. In this study, a collagen type I biomaterial was used to expand and enhance the function of human peripheral blood CD34+ cells, and potential underlying mechanisms were examined...
Article
We have prepared and tested in vivo a novel nanoengineered hybrid electroconductive cardiac patch for treating the infarcted myocardium. Of the prepared and tested patches, only those containing spherical nanogold were able to increase connexin-43 expression in neonatal rat cardiomyocytes cultured under electrical stimulation. In vivo data indicate...
Article
Full-text available
In living organisms, biofilms are defined as complex communities of bacteria residing within an exopolysaccharide matrix that adheres to a surface. In the clinic, they are typically the cause of chronic, nosocomial, and medical device-related infections. Due to the antibiotic-resistant nature of biofilms, the use of antibiotics alone is ineffective...
Article
Background. Myocardial infarction (MI) stimulates production of the toxic metabolite methylglyoxal (MG), and its accumulation postMI contributes to reduced vascularization and cardiac dysfunction. We hypothesized that using a collagen hydrogel to deliver fisetin, a methylglyoxal scavenger, would improve post-MI vascularity, morphology and function....
Chapter
The currently available therapeutic strategies for end-stage congestive heart failure are all invasive surgical approaches, including implantable devices and, ultimately, heart transplantation. However, these surgical treatments are unable to restore damaged tissue or provide functional restoration due to the limited intrinsic self-regenerative cap...
Chapter
Age-associated diseases are becoming more prevalent as the worldwide population is getting older. This chapter reviews the current developments in nanotechnology directed at combating the rise of diseases and ailments stemming from ageing. The major complications that are discussed are cancers, cardiovascular disease, and neurodegeneration. Medical...
Article
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Human endothelial colony-forming cells (ECFCs) represent a promising source of adult stem cells for vascular repair, yet their regenerative capacity is limited. Here, we set out to understand the molecular mechanism restricting the repair function of ECFCs. We found that key pro-angiogenic pathways are repressed in ECFCs due to the presence of biva...
Chapter
The biomedical properties of nanoparticulated silver have been widely studied in the last decade; however, still there are concerns regarding its long-term toxicity. Recent developments in nanoparticle fabrication and surface manipulation, using different biomolecules, have allowed the preparation of nontoxic nanosilver. Thus, nanosilver has been s...
Article
Full-text available
Advanced glycation end-products (AGEs) have been associated with poorer outcomes after myocardial infarction (MI), and linked with heart failure. Methylglyoxal (MG) is considered the most important AGE precursor, but its role in MI is unknown. In this study, we investigated the involvement of MG-derived AGEs (MG-AGEs) in MI using transgenic mice th...
Article
We report for the first time the preparation of a fibrous material composed of surface grafted spherical nanosilver and collagen using one-step electrospinning. The resulting composite showed comparable morphology to the control without nanosilver, but had improved electrical conductivity. Under electrical stimulation, fibrous materials containing...
Article
Using a combination of methacrylated collagen and the photosensitizer rose Bengal, a new light-activated biomimetic material for tissue sutureless bonding was developed. This formulation was cross-linked using green light. In vivo tests in mice demonstrate the suitability of the material for sutureless wound closure.
Chapter
Cardiac regenerative therapy has received attention as a potentially revolutionary approach for treating the damaged heart. The mouse model of myocardial infarction (MI) remains one of the most common tools for the evaluation of such new therapies. Typically, intramyocardial administration of cells or biomaterials in mice is performed by an open-ch...
Article
Introduction: The diseased host milieu, such as endothelial dysfunction (ED), decreased NO bioavailability, and ischemic/inflammatory post-MI environment, hamper the clinical success of existing cardiac regenerative therapies. Area covered: In this article, current strategies including pharmacological and nonpharmacological approaches for improving...
Article
Sprayable formulations of AgNPs were prepared by exchanging citrate capping agents with LL37-SH peptides. The AgNP@LL37 material was then combined with type I collagen to form a stable film once sprayed. The AgNP@LL37 spray prevented Pseudomonas aeruginosa (P. aeruginosa) proliferation and eradicated a P. aeruginosa biofilm, while being non-toxic f...
Article
Introduction: The feasibility and safety of bone marrow cell (BMC) therapy for cardiac repair following myocardial infarction has been demonstrated in clinical studies, albeit with relatively modest structural and functional benefits. In response to the shortcomings of BMC therapy, the use of biomaterials to enhance cell transplantation is being i...
Article
Full-text available
The healthy heart comprises many different cell types that work together to preserve optimal function. However, in a diseased heart the function of one or more cell types is compromised which can lead to many adverse events, one of which is myocardial infarction (MI). Immediately after MI, the cardiac environment is characterized by excessive cardi...
Article
Nanomaterials have attracted the interest of tissue engineers for the last two decades. Their unique properties make them promising for de-novo fabrication of bio-inspired hybrid/composite materials with improved regenerative properties, including the capacity for electric conductivity and the provision of antimicrobial properties, for example. How...
Article
First generation cardiac stem cell products provide indirect cardiac repair but variably produce key cardioprotective cytokines, such as stromal-cell derived factor 1α, which opens the prospect of maximizing up-front paracrine-mediated repair. The mesenchymal sub-population within explant derived human cardiac stem cells underwent lentiviral mediat...
Article
Full-text available
The mechanisms for the development of diabetic cardiomyopathy remain largely unknown. Methylglyoxal (MG) can accumulate and promote inflammation and vascular damage in diabetes. We examined if overexpression of glyoxalase-1 (GLO1; MG-metabolizing enzyme) in macrophages and the vasculature could reduce MG-induced inflammation and prevent ventricular...
Article
Despite the broad antimicrobial and anti-inflammatory properties of silver nanoparticles (AgNPs), their use in bioengineered corneal replacements or bandage contact lenses has been hindered due to their intense yellow coloration. In this communication, we report the development of a new strategy to pre-stabilize and incorporate AgNPs with different...
Conference Paper
Background: While cardiac stem cells are emerging as a promising therapy for ischemic cardiomyopathy (ICM), the influence of advanced donor age and ICM on the regenerative performance of these cells are unknown. Hypothesis: Advanced donor age and a history of ICM combine to limit the regenerative performance of explant-derived cells (EDCs) through...
Article
Background: Matricellular proteins are non-structural ECM proteins with important wound healing effects. They regulate cell function through interactions with cell surface receptors, enzymes and structural proteins such as collagen. The aim of this study was to examine the effects of matricellular protein CCN1 delivery in a mouse myocardial infarct...
Article
Full-text available
The increasing number of multidrug resistant bacteria has revitalized interest in seeking alternative sources for controlling bacterial infection. Silver nanoparticles (AgNPs), are amongst the most promising candidates due to their wide microbial spectrum of action. In this work, we report on the safety and efficacy of the incorporation of collagen...
Chapter
Full-text available
Cardiac disease is one of the most common causes of death in the world. Currently, surgically invasive therapies, such as implantable devices and, ultimately, heart transplantation, are the only remedies for end-stage congestive heart disease, which constitutes the final common pathway for all cardiac disorders. However, these surgical treatments a...