Eric Lee's scientific contributions
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Publications (2)
The initial step in Next Generation Sequencing is to construct a library from genomic DNA. To gain the optimum result, extracted DNA must be of high molecular weight with limited degradation. High-throughput sequencing projects, such as the 100K Pathogen Genome Project, require methods to rapidly assess the quantity and quality of genomic DNA extra...
Shearing of bacterial gDNA within a specific size range prior to sequencing library construction is a critical step in Next Generation Sequencing workflows. The quality control of the sheared bacterial gDNA is required in large multiplexed formats for large volume workflows, such as those used in the 100K Pathogen Genome Sequencing Project. Using t...
Citations
... High-molecular-weight DNA was extracted using the QIAamp DNA minikit (Qiagen, Valencia, CA). The integrity of high-molecular-weight DNA was determined using a 2200 TapeStation with genomic DNA ScreenTape (Agilent Technologies, Santa Clara, CA) as previously described (52). ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/459552303915013-1486577215732_Q64/Nguyet-Kong.jpg)
... Cultures were grown on 1.5% Luria-Bertani agar (Difco, Franklin Lakes, NJ, USA), with 10 g/mL of chloramphenicol at 37°C, and then lysed (8). Genomic DNA was extracted (9), checked for quality (10), and fragmented (11). The 350-to 500-bp libraries (12,13) were indexed (96 genomes/lane) and sequenced (Illumina HiSeq 3000; 150-bp paired-end) (14)(15)(16) at the UC Davis DNA Technologies Core. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/515605791232000-1499941408963_Q64/Narine-Arabyan.jpg)
