Enqin Li's research while affiliated with University of Macau and other places

Publications (27)

Article
We have previously demonstrated that mesenchymal stromal/stem cells (MSCs) in spheroids (MSCsp) tolerate ambient and hypoxic conditions for a prolonged time. Local administration of MSCsp, but not dissociated MSCs (MSCdiss), promotes wound healing and relieves multiple sclerosis and osteoarthritis in mice and monkeys. These findings indicate an adv...
Article
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Mesenchymal stem cells (MSCs) as a therapeutic promise are often quickly cleared by innate immune cells of the host including natural killer (NK) cells. Efforts have been made to generate immune-escaping human embryonic stem cells (hESCs) where T cell immunity is evaded by defecting β-2-microglobulin (B2M), a common unit for human leukocyte antigen...
Article
Mesenchymal stem cells (MSCs) derived from somatic tissues have been used to promote lipotransfer, a common practice in cosmetic surgery. However, the effect of lipotransfer varies, and the mechanism of action remains vague. To address these questions, we differentiated human embryonic stem cells, a stable and unlimited source, into MSCs (EMSCs). T...
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The outbreak of Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2), has thus far killed over 3,000 people and infected over 80,000 in China and elsewhere in the world, resulting in catastrophe for humans. Similar to its homologous virus, SARS-CoV, which caused SARS in thousands of peop...
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Mesenchymal stem cells (MSC) derived from adult tissues effectively promote wound healing. However, MSC quality varies, and the quantity of MSC is limited, as MSC are acquired through donations. Moreover, the survival and functioning of dissociated MSC delivered to an inflammatory lesion are subject to challenges. Methods: Here, spheres (EMSCSp) g...
Article
Despite of the long discrepancy over their definition, heterogeneity, and functions, mesenchymal stromal/stem cells (MSCs) have proved to be a key player in tissue repair and homeostasis. Generally, somatic tissue‐derived MSCs (st‐MSCs) are subject to quality variations related to donated samples and biosafety concern for transmission of potential...
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Nonhuman primate experimental autoimmune encephalomyelitis (EAE) is a valuable model for multiple sclerosis, an inflammatory demyelinating disease in the central nervous system (CNS). Human embryonic stem cell-derived mesenchymal stem cells (EMSC) are effective in treating murine EAE. Yet, it remains unknown whether the EMSC efficacy is translatabl...
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Mesenchymal stem cells (MSC) have been derived from a variety of tissues, and cultured either in animal serum-containing (SC) or serum-free (SF) media. We have previously derived MSC from human embryonic stem cells via an intermediate trophoblast step (named EMSC), which also have immunosuppressive and therapeutic effects on animal models of autoim...
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Human embryonic stem cell (hESC) derived mesenchymal stem cells (EMSC) are efficacious in treating a series of autoimmune, inflammatory, and degenerative diseases in animal models. However, all the EMSC derivation methods reported so far rely on two-dimensional (2D) culture systems, which are inefficient, costive and difficult for large-scale produ...
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Background: Multiple sclerosis (MS) is an autoimmune and demyelinating disease. Genome-wide association studies have shown that MS is associated with many genetic variants in some human leucocyte antigen genes and other immune-related genes, however, those studies were mostly specific to Caucasian populations. We attempt to address whether the same...
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Marfan syndrome (MFS) is a connective tissue disorder caused by mutations in FBN1 gene, which encodes a key extracellular matrix protein FIBRILLIN-1. The haplosufficiency of FBN1 has been implicated in pathogenesis of MFS with manifestations primarily in cardiovascular, muscular, and ocular tissues. Due to limitations in animal models to study the...
Article
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Human Stem cells are vulnerable to unfavorable conditions, and their transportation relies on costly and inconvenient cryopreservation. We report here that human mesenchymal stem cells (MSC) in spheroids survived ambient conditions (AC) many days longer than in monolayer. Under AC, the viability of MSC in spheroids remained >90% even after seven da...
Article
Full-text available
Mesenchymal stem/stromal cells (MSCs) have great clinical potential in modulating inflammation and promoting tissue repair. Human embryonic stem cells (hESCs) have recently emerged as a potentially superior cell source for MSCs. However, the generation methods reported so far vary greatly in quality and efficiency. Here we describe a novel method t...

Citations

... New discoveries in mechanisms of immune homeostasis also provide new avenues for SCDI engineering. The ubiquitin editing enzyme A20, encoded by TNFAIP3, and FasL have been shown to play a dominant role in protecting islet allografts (45,46) together with a short course of rapamycin. A20 overexpression inhibits the expression of inflammatory mediators and raises inflammatory signaling thresholds which promotes the development of antigen specific Tregs supporting immune tolerance and islet survival (45). ...
... MSCs have been implicated as an excellent cell type to alleviate dysregulated immunity through the secretion of anti-inflammatory cytokines and the expression of immunomodulatory surface proteins [119,120]. MSCs are easy to culture, scale up, and characterize in vitro and can be easily tested in animal models [121][122][123][124]. Furthermore, MSCs are low in immunogenicity in the allogeneic engraftment [125,126]. Pre-conditioning of MSCs via hypoxic culture or treatment with an inflammatory stimulus such as lipopolysaccharide enhances their immunomodulatory effects due to increased secretion of anti-inflammatory cytokines, including NO, IL-10, and TGFβ, and decreased secretion of inflammatory cytokines, including IL-1, IL-6, and TNFα [127]. Finally, MSCs can directly modulate T cell activity by suppressing effector T cell proliferation and increasing regulatory T cell expansion through elevated expression of immune-modulatory ligands such as programmed cell death ligand-1 [123,128]. ...
... The SARS-CoV-2 pandemic has had a massive impact worldwide over the past two years, with over 620,000,000 infections and over 6,500,000 deaths as of October 2022 [1]. Patients with end-stage renal disease (ESRD) in renal replacement therapy, such as renal transplantation, peritoneal dialysis, and hemodialysis, are a population with higher infection rates and adverse outcomes, including hospitalizations, requirements of mechanical ventilation and deaths, compared to the general population [2][3][4][5]. ...
... In the pilot study discussed above, no serious adverse effect or death directly related to the implantation of stem cells. Concerns about the biosafety of stem cell transplantation have been reported in many other studies, and the behavior of transplanted stem cells can be guaranteed in many ways [131]. Nonetheless, many factors still challenge the establishment of stem cell based therapy in treating OD, such as the source, density and quality of stem cells, the administration route, dosage and frequency. ...
... These morphological traits are comparable to human MSCs isolated from adult tissues and cultured atop TCPS substrates. The in vitro expansion rate (Fig. 1e, left) was consistent across all iMSC lines and iMSCs divided faster than adult MSCs in accordance with previously published data 22,23 . Interestingly, in vitro expansion over ten passages also results in a reproducible and progressive increase in average cellular length, which is a phenomenon also observed in tissue derived MSCs (Fig. 1e, right). ...
... Moreover, the numbers of harvested MSCs and their in vitro expansion were a challenge [18,19]. The successful isolation and culture of embryonic stem cells (ESCs) in vitro [20], which are capable of unlimited selfrenewal and differentiation into all cell lineages, opened a new avenue for MSC derivation [21][22][23]. ...
... The scientific community continues to debate the distinction between zygote, embryo, and fetus, as well as the moment when life begins [189][190][191]. Though increasingly popular, little research has been done on the ethical issues raised by using stem cells to treat neurological diseases [83,81]. ...
... In addition to hearing loss, rare missense mutations in TRIOBP-4 (and longer splice variants) have also been detected in a patient with multiple sclerosis (A322S mutation) [63] and in a patient with developmental delay, visual impairment, muscle weakness, hypotonia, clinodactyly, and mild hearing impairment (R1078C mutation) [64]. ...
... MSCs have been implicated as an excellent cell type to alleviate dysregulated immunity through the secretion of anti-inflammatory cytokines and the expression of immunomodulatory surface proteins [119,120]. MSCs are easy to culture, scale up, and characterize in vitro and can be easily tested in animal models [121][122][123][124]. Furthermore, MSCs are low in immunogenicity in the allogeneic engraftment [125,126]. ...
... Genetics and epigenetics could also affect the target tissues of the cellular therapies. For example, some individuals with Marfan's Syndrome or the spectrum of Ehlers-Danlos Syndrome [194] may appear to have mutations in some of their ECM proteins that impair function and increase risk for injury or tissue failure reviewed in [193,[195][196][197]. Thus, the outcome of the cellular therapy may not be optimal when using autologous cells and allogeneic cells may be preferred [198] or correcting the MSC via in vitro alterations [195]. ...